Cloning and characterization of a gene (mspA) encoding the major sheath protein of Treponema maltophilum ATCC 51939(T).

Abstract:

:The major sheath protein-encoding gene (mspA) of the oral spirochete Treponema maltophilum ATCC 51939(T) was cloned by screening a genomic library with an anti-outer membrane fraction antibody. The mspA gene encodes a precursor protein of 575 amino acids with a predicted molecular mass of 62.3 kDa, including a signal peptide of 19 amino acids. The native MspA formed a heat-modifiable, detergent- and trypsin-stable complex which is associated with the outer membrane. Hybridization with an mspA-specific probe showed no cross-reactivity with the msp gene from Treponema denticola.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Heuner K,Choi BK,Schade R,Moter A,Otto A,Göbel UB

doi

10.1128/JB.181.3.1025-1029.1999

subject

Has Abstract

pub_date

1999-02-01 00:00:00

pages

1025-9

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

181

pub_type

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