Abstract:
:The slime polysaccharides produced by Pseudomonas aeruginosa isolated from a variety of human infections were investigated. Slime production in culture seemed optimal when adequate amounts of carbohydrate were present and under conditions of either high osmotic pressure or inadequate protein supply. The polysaccharides produced by the organisms were similar to each other, to the slime of Azotobacter vinelandii, and to seaweed alginic acids. They were composed of beta-1,4-linked d-mannuronic acid residues and variable amounts of its 5-epimer l-guluronic acid. All bacterial polymers contained o-acetyl groups which are absent in the alginates. The polysaccharides differed considerably in the ratio of mannuronic to guluronic acid content and in the number of o-acetyl groups. The particular composition of the slime was not found to be characteristic for the disease process from which the mucoid variants of P. aeruginosa were obtained.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Evans LR,Linker Adoi
10.1128/JB.116.2.915-924.1973subject
Has Abstractpub_date
1973-11-01 00:00:00pages
915-24issue
2eissn
0021-9193issn
1098-5530journal_volume
116pub_type
杂志文章abstract::Forty-five strains, representing 18 species of the genus Bacillus, were surveyed for production of hemolysin against rabbit erythrocytes. Broth cultures of B. cereus, B. alvei, and B. laterosporus contained lysins that closely resembled streptolysin O. B. subtilis and a single strain of B. cereus may produce lysins ha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.5.1541-1543.1967
更新日期:1967-05-01 00:00:00
abstract::The lasA gene (whose product is involved in the production of extracellular elastolytic activity) was isolated from a genomic bank containing DNA from Pseudomonas aeruginosa FRD1. Recombinant plasmid pELA1, containing the lasA gene, complemented the temperature-sensitive elastase mutation (lasA1) in P. aeruginosa PAO-...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.3.1349-1351.1987
更新日期:1987-03-01 00:00:00
abstract::The CO dehydrogenase structural genes (cox) and orf4 are clustered in the transcriptional order coxM--> coxS--> coxL--> orf4 on the 128-kb megaplasmid pHCG3 of the carboxidotroph Oligotropha carboxidovorans OM5. Sequence analysis suggested association of molybdopterin cytosine dinucleotide and flavin adenine dinucleot...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.8.2197-2203.1995
更新日期:1995-04-01 00:00:00
abstract::Kimler, Alexander (National Institutes of Health, Bethesda, Md.). Fluorescein amine media for rapid differentiation of staphylococci. J. Bacteriol. 82:106-109. 1961.-By modifying conventional media to include blood, mannitol, and fluorescein amine, staphylococci may be isolated and identified directly. Staphylococcus ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.82.1.106-109.1961
更新日期:1961-07-01 00:00:00
abstract::The high-potential 4Fe-4S* center ofAzotobacter vinelandii ferredoxin I has been titrated potentiometrically by a reductive procedure. The absorbance decrease at 510 nm accompanying the reduction of the high-potential center titrated with an Em of 320 mV (n = 1). The low-potential 4Fe-4S* center was titrated by using ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.2.822-824.1978
更新日期:1978-11-01 00:00:00
abstract::The flavodoxins constitute a highly conserved family of small, acidic electron transfer proteins with flavin mononucleotide prosthetic groups. They are found in prokaryotes and in red and green algae, where they provide electrons at low potentials for the reduction of nitrogen by nitrogenase, for the light-dependent r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.5.1729-1737.1991
更新日期:1991-03-01 00:00:00
abstract::The P(styA) promoter of Pseudomonas sp. strain Y2 controls expression of the styABCD genes, which are required for the conversion of styrene to phenylacetate, which is further catabolized by the products of two paa gene clusters. Two PaaX repressor proteins (PaaX1 and PaaX2) regulate transcription of the paa gene clus...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00176-06
更新日期:2006-07-01 00:00:00
abstract::Salmonella enterica encodes a type III secretion system within a pathogenicity island located at centisome 63 that is essential for virulence. All type III secretion systems require the function of a family of low-molecular-weight proteins that aid the secretion process by acting as partitioning factors and/or secreti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.8.2262-2268.2000
更新日期:2000-04-01 00:00:00
abstract::We have constructed a promoter-probe expression vector for Bacillus subtilis. This plasmid, pCED6, can be used to fuse various DNA sequences to the structural gene of Escherichia coli beta-galactosidase, permitting analysis of the promoter activity of such sequences. pCED6 replicates and confers drug resistances in bo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.157.3.965-967.1984
更新日期:1984-03-01 00:00:00
abstract::We identified Mg2+-responsive promoters of the phoPQ, mgtA, and mgrB genes of Escherichia coli K-12 by S1 nuclease analysis. Expression of these genes was induced by magnesium limitation and depended on PhoP and PhoQ. The transcription start sites were also determined, which allowed us to find a (T/G)GTTTA direct repe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.17.5516-5520.1999
更新日期:1999-09-01 00:00:00
abstract::Radiorespirometric and enzymatic analyses reveal that glucose-grown cells of Rhizobium japonicum isolates I-110 and L1-110, both derivatives of R. japonicum strain 3I1b110, possess an active tricarboxylic acid cycle and metabolize glucose by simultaneous operation of the Embden-Meyerhof-Parnas and Entner-Doudoroff pat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.131.1.179-187.1977
更新日期:1977-07-01 00:00:00
abstract::Two proteins that bind penicillin were observed in Escherichia coli infected with lambda phages 141, 142, 650, and 651 from the Kohara genomic library. These phages carry chromosomal DNA fragments that do not contain any known penicillin binding protein (PBP) genes, indicating that unrecognized gene products were exhi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.19.6112-6121.1997
更新日期:1997-10-01 00:00:00
abstract::A noncomposite transposon, designated Tn5541, was isolated from strain Fd33 of the filamentous cyanobacterium Fremyella diplosiphon UTEX 481. Sequence analysis showed that Tn5541 is structurally and genetically very similar to Tn5469, which is also endogenous to F. diplosiphon. Both Tn5469 and Tn5541 encode homologous...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.22.6059-6063.1998
更新日期:1998-11-01 00:00:00
abstract::A segment of Escherichia coli DNA that contained a discontinuity of homology with Salmonella typhimurium DNA was isolated. The segment, 1,430 base pairs long, was derived from one end of the lac "loop," a region of about 12 kilobase pairs of E. coli DNA, including the lac operon which has no detectable homology with S...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.159.2.618-623.1984
更新日期:1984-08-01 00:00:00
abstract::Rhodopseudomonas capsulata required potassium (or rubidium or cesium as analogs of potassium) for growth. These cations were actively accumulated by the cells by a process following Michaelis-Menten saturation kinetics. The monovalent cation transport system had Km's of 0.2 mM K+, 0.5 mM Rb+, and 2.6 mM Cs+. The rates...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.3.1314-1322.1978
更新日期:1978-03-01 00:00:00
abstract::Cell-free extract (crude extract) of Agrobacterium tumefaciens grown on d-glucuronate or d-glucarate converts d-glucarate and galactarate to a mixture of 2-keto-3-deoxy- and 4-deoxy-5-keto-d-glucarate. These compounds are then converted by partially purified crude extract to an intermediate tentatively identified as 2...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.102.1.85-96.1970
更新日期:1970-04-01 00:00:00
abstract::The 3.1-Mb genome of an outbreak methicillin-resistant Staphylococcus aureus (MRSA) strain (TW20) contains evidence of recently acquired DNA, including two large regions (635 kb and 127 kb). The strain is resistant to a wide range of antibiotics, antiseptics, and heavy metals due to resistance genes encoded on mobile ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01255-09
更新日期:2010-02-01 00:00:00
abstract::The 5 to 10 peritrichously inserted complex flagella of Rhizobium meliloti MVII-1 were found to form right-handed flagellar bundles. Bacteria swam at speeds up to 60 microns/s, their random three-dimensional walk consisting of straight runs and quick directional changes (turns) without the vigorous angular motion (tum...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.7.3146-3150.1987
更新日期:1987-07-01 00:00:00
abstract::SwmB is required for swimming motility in Synechococcus sp. strain WH8102. This highly repetitive 1.12-MDa polypeptide is associated with the cell surface, where it is arranged in a punctate manner. Inactivation of swmB does not affect the localization of SwmA, an S-layer protein also required for swimming. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01500-06
更新日期:2007-02-01 00:00:00
abstract::The host factor (HF-I) for phage Q beta RNA replication is a small protein of 102 amino acid residues encoded by the hfq gene at 94.8 min on the Escherichia coli chromosome. The synthesis rate of HF-I at the exponential-growth phase is higher than at the stationary phase, and it increases concomitantly with the increa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.2.531-534.1994
更新日期:1994-01-01 00:00:00
abstract::Spores were isolated from the filamentous cyanobacterium Anabaena cylindrica, and their deoxyribonucleic acid, ribonucleic acid, and protein compositions were determined. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.2.1154-1155.1977
更新日期:1977-02-01 00:00:00
abstract::The SsuDAT1I restriction-modification (R-M) system, which contains two methyltransferases and two restriction endonucleases with recognition sequence 5'-GATC-3', was first found in a field isolate of Streptococcus suis serotype 2. Isoschizomers of the R-M system were found in the same locus between purH and purD in a ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.183.18.5436-5440.2001
更新日期:2001-09-01 00:00:00
abstract::Enterococcus faecalis ATCC 29212, a vancomycin-sensitive strain, has been extensively used as a representative control strain for clinical and laboratory experiments. Here we report the draft genome and annotation of this strain, containing 3,027,060 bp, with a G+C content of 37.2% in 126 contigs (≥ 500 bp). ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01423-12
更新日期:2012-11-01 00:00:00
abstract::The manganese-containing isozyme of superoxide dismutase (MnSOD) is synthesized by Escherichia coli only during aerobiosis, in accordance with the fact that superoxide can be formed only in aerobic environments. In contrast, E. coli continues to synthesize the iron-containing isozyme (FeSOD) even in the absence of oxy...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.24.7653-7658.1994
更新日期:1994-12-01 00:00:00
abstract::In Escherichia coli, the following genes are involved in motility and chemotaxis. The H gene is the structural gene for flagellin. Mutation in the mot gene results in paralysis of the flagella, and mutation in the fla genes leads to an absence of flagella. The cheA, cheB, and cheC genes are required for chemotaxis. Th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.1.156-161.1969
更新日期:1969-01-01 00:00:00
abstract::A Pediococcus cerevisiae mutant that actively accumulated folate (PteGlu), in contrast to the wild-type, was also found to exhibit changes in the pattern of uptake of 5-methyl-tetrahydrofolate (5-CH3-H4PteGlu) and amethopterin. Most of the 5-CH3-H4PteGlue accumulated through a glucose- and temperature-dependent proces...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.123.2.400-406.1975
更新日期:1975-08-01 00:00:00
abstract::Bacterial Gre factors associate with RNA polymerase (RNAP) and stimulate intrinsic cleavage of the nascent transcript at the active site of RNAP. Biochemical and genetic studies to date have shown that Escherichia coli Gre factors prevent transcriptional arrest during elongation and enhance transcription fidelity. Fur...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00086-11
更新日期:2011-06-01 00:00:00
abstract::Pseudomonas aeruginosa produced alginate and elevated algD (encoding GDPmannose 6-dehydrogenase) transcription under strict anaerobic conditions, especially when using nitrate as a terminal electron acceptor. Purified alginate added to bacterial suspensions caused a decrease in growth, suggesting that alginate contrib...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.24.7322-7325.1996
更新日期:1996-12-01 00:00:00
abstract::gldA, the structural gene for the NAD(+)-dependent glycerol dehydrogenase, was mapped at 89.2 min on the Escherichia coli linkage map, cotransducible with, but not adjacent to, the glpFKX operon encoding the proteins for the uptake and phosphorylation of glycerol. gldA was cloned, and its position on the physical map ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.6.1796-1800.1994
更新日期:1994-03-01 00:00:00
abstract::The spores of Bacillus subtilis show remarkable resistance to many environmental stresses, due in part to the presence of an outer proteinaceous structure known as the spore coat. GerQ is a spore coat protein essential for the presence of CwlJ, an enzyme involved in the hydrolysis of the cortex during spore germinatio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.17.5567-5575.2004
更新日期:2004-09-01 00:00:00