Isolation and characterization of a Saccharomyces cerevisiae mutant deficient in pyruvate kinase activity.

abstract：:The gene tyrP, which codes for a component of the tyrosine-specific transport system, has been localized on the Escherichia coli K-12 chromosome at min 42. A tyrP-lac operon fusion was constructed and used to isolate mutants that have altered expression from the tyrP promoter. All putative tyrP operator mutations were...

journal_title：Journal of bacteriology

authors： Kasian PA,Pittard J

更新日期：1984-10-01 00:00:00

abstract：:The molecular mechanisms of regulation of the genes involved in the biosynthesis of cysteine are poorly characterized in Bacillus subtilis and other gram-positive bacteria. In this study we describe the expression pattern of the B. subtilis cysH operon in response to sulfur starvation. A 6.1-kb polycistronic transcrip...

journal_title：Journal of bacteriology

authors： Mansilla MC,Albanesi D,de Mendoza D

更新日期：2000-10-01 00:00:00

abstract：:Accumulation of certain nonmetabolizable sugar-phosphates (including α-methyl glucoside-6-phosphate) in Escherichia coli is growth inhibitory and elicits the glucose-phosphate stress response. The transcription factor SgrR activates transcription of the small RNA SgrS under stress conditions. SgrS represses translatio...

journal_title：Journal of bacteriology

authors： Sun Y,Vanderpool CK

更新日期：2011-01-01 00:00:00

abstract：:Spontaneous mutants that were resistant to zinc were isolated from Alcaligenes eutrophus CH34 containing either the native plasmid pMOL28 or a derivative derepressed for its self-transfer, pMOL50. With the cured plasmid-free derivative of CH34, strain AE104, such mutants were not detected. The mutations, which were sh...

journal_title：Journal of bacteriology

authors： Collard JM,Provoost A,Taghavi S,Mergeay M

更新日期：1993-02-01 00:00:00

abstract：:Nitrate reductase extracted from the membrane of Escherichia coli by alkaline heat treatment was purified to homogeneity and used to prepare specific antibody. Nitrate reductase, precipitated by this antibody from Triton extracts of the membrane, contained a third subunit not present in the purified enzyme used to pre...

journal_title：Journal of bacteriology

authors： MacGregor CH

更新日期：1975-03-01 00:00:00

abstract：:Evidence is reported here that alkalinization of Clostridium acetobutylicum cytoplasm involves hydrogenase activity. A decrease of in vivo hydrogenase activity is accompanied by intracellular accumulation of protons leading to a negative (interior acidic) pH gradient. However, the organism is able to maintain a consta...

journal_title：Journal of bacteriology

authors： Girbal L,Vasconcelos I,Soucaille P

更新日期：1994-10-01 00:00:00

abstract：:The gene for beta-amylase was isolated from Bacillus polymyxa by molecular cloning in B. subtilis. B. subtilis cells containing this gene express and secrete an amylase which resembles the B. polymyxa beta-amylase and barley beta-amylase in terms of the products it generates during carbohydrate hydrolysis. Starch hydr...

journal_title：Journal of bacteriology

authors： Friedberg F,Rhodes C

更新日期：1986-03-01 00:00:00

abstract：:We report on the cloning and characterization of the rfb gene cluster of the O75 lipopolysaccharide from a urinary tract isolate of Escherichia coli. Deletion cloning defined the minimum region of DNA that expressed the O75 antigen in E. coli host strains to be on a 12.4-kb insert. However, the E. coli strain expressi...

journal_title：Journal of bacteriology

authors： Batchelor RA,Haraguchi GE,Hull RA,Hull SI

更新日期：1991-09-01 00:00:00

abstract：:Common histidine-to-aspartate (His-to-Asp) phosphorelay signaling systems involve three types of signaling components: a sensor His kinase, a response regulator, and a histidine-containing phosphotransfer (HPt) protein. In the fission yeast Schizosaccharomyces pombe, two response regulators, Mcs4 and Prr1, have been i...

journal_title：Journal of bacteriology

authors： Aoyama K,Mitsubayashi Y,Aiba H,Mizuno T

更新日期：2000-09-01 00:00:00

abstract：:The ccd mechanism specified by the ccdA and ccdB genes of the mini-F plasmid determines fate of plasmid-free segregants in Escherichia coli (Jaffé et al., J. Bacteriol. 163:841-849, 1985). The killing function in plasmid-free segregants by the ccd mechanism did not affect cell growth of coexisting cells in the same cu...

journal_title：Journal of bacteriology

authors： Hiraga S,Jaffé A,Ogura T,Mori H,Takahashi H

更新日期：1986-04-01 00:00:00

abstract：:The F-plasmid-encoded TraI protein, also known as DNA helicase I, is a bifunctional protein required for conjugative DNA transfer. The enzyme catalyzes two distinct but functionally related reactions required for the DNA processing events associated with conjugation: the site- and strand-specific transesterification (...

journal_title：Journal of bacteriology

authors： Matson SW,Ragonese H

更新日期：2005-01-01 00:00:00

abstract：:Pseudomonas sp. strain PCL1171 displays colony phase variation between opaque phase I and translucent phase II colonies, thereby regulating the production of secondary metabolites and exoenzymes. Complementation and sequence analysis of 26 phase II mutants and of 13 wild-type phase II sectors growing out of phase I co...

journal_title：Journal of bacteriology

authors： van den Broek D,Chin-A-Woeng TF,Bloemberg GV,Lugtenberg BJ

更新日期：2005-01-01 00:00:00

abstract：:The fate of 35-S during anaerobic metabolism of [35-S]sulfate, [35-S]thiosulfate, and [35-S]sulfate plus unlabeled thiosulfate by washed cell suspensions of Desulfovibrio spp, and of [35-S]thiosulfate by growing D. desulfuricans was examined. The results appear to be inconsistent with the hypothesis that thiosulfate i...

journal_title：Journal of bacteriology

authors： Chambers LA,Trudinger PA

更新日期：1975-07-01 00:00:00

abstract：:In a search for genes that produce hypersensitivity to cadmium salts in Escherichia coli, random transposon mutagenesis with TnphoA was used. One of the mutant strains obtained was sensitive to Cd2+ and Zn2+. Sequence analysis showed that the TnphoA insertion was located in the dsbA gene coding for a periplasmic prote...

journal_title：Journal of bacteriology

authors： Rensing C,Mitra B,Rosen BP

更新日期：1997-04-01 00:00:00

abstract：:Acid-soluble spore proteins (ASSPs) comprise about 5% of the total protein of mature spores of different Bacillus subtilis strains. They consist of three abundant species, alpha, beta, and gamma, four less abundant species, and several minor species, alpha, beta, and gamma make up about 18, 18 and 36%, respectively, o...

journal_title：Journal of bacteriology

authors： Johnson WC,Tipper DJ

更新日期：1981-06-01 00:00:00

abstract：:Mycoplasma pneumoniae exhibits a novel form of gliding motility that is mediated by the terminal organelle, a differentiated polar structure. Given that genes known to be involved in gliding in other organisms are absent in M. pneumoniae, random transposon mutagenesis was employed to generate mutants with gliding-defi...

journal_title：Journal of bacteriology

authors： Page CA,Krause DC

更新日期：2013-04-01 00:00:00

abstract：:Entry into sporulation in Bacillus subtilis is characterized by the formation of a polar septum, which asymmetrically divides the developing cell into forespore (the smaller cell) and mother cell compartments, and by migration of replication origin regions to extreme opposite poles of the cell. Here we show that polar...

journal_title：Journal of bacteriology

authors： Graumann PL,Losick R

更新日期：2001-07-01 00:00:00

abstract：:A widely distributed family of small regulators, called C proteins, controls a subset of restriction-modification systems. The C proteins studied to date activate transcription of their own genes and that of downstream endonuclease genes; this arrangement appears to delay endonuclease expression relative to that of th...

journal_title：Journal of bacteriology

authors： Knowle D,Lintner RE,Touma YM,Blumenthal RM

更新日期：2005-01-01 00:00:00

abstract：:Mycoplasma virus L2 is subject to host-specific restriction and modification in Acholeplasma laidlawii strains JA1 and K2. We have examined the DNAs from both host cells and viruses propagated on these strains with respect to susceptibility to cleavage by restriction endonucleases and for DNA base modifications. We sh...

journal_title：Journal of bacteriology

authors： Dybvig K,Swinton D,Maniloff J,Hattman S

更新日期：1982-09-01 00:00:00

abstract：:Double-stranded, 1.9-kilobase-pair (kbp) DNA molecules were found in 18 strains representing three pathogenic races of Fusarium oxysporum f. sp. conglutinans. The DNA element (pFOXC1) from a race 1 strain and the DNA element (pFOXC2) from a race 2 strain were shown by restriction endonuclease mapping to be linear. pFO...

journal_title：Journal of bacteriology

authors： Kistler HC,Leong SA

更新日期：1986-08-01 00:00:00

abstract：:Deoxyribonucleic acid (DNA)-dependent ribonucleic acid (RNA) polymerase (EC 2.7.7.6) from Acinetobacter calcoaceticus was purified to apparent homogeneity and its properties were compared with those of the Escherichia coli B enzyme. The molecular weights of the two native active enzymes as well as their alpha and beta...

journal_title：Journal of bacteriology

authors： Kleppe RK,Kleppe K

更新日期：1976-02-01 00:00:00

abstract：:The pattern of peptidoglycan (murein) segregation in cells of Escherichia coli with impaired activity of the morphogenetic proteins penicillin-binding protein 2 and RodA has been investigated by the D-cysteine-biotin immunolabeling technique (M. A. de Pedro, J. C. Quintela, J.-V. Höltje, and H. Schwarz, J. Bacteriol. ...

journal_title：Journal of bacteriology

authors： de Pedro MA,Donachie WD,Höltje JV,Schwarz H

更新日期：2001-07-01 00:00:00

abstract：:The enterohepatic Helicobacter species Helicobacter hepaticus colonizes the murine intestinal and hepatobiliary tract and is associated with chronic intestinal inflammation, gall stone formation, hepatitis, and hepatocellular carcinoma. Thus far, the role of H. hepaticus motility and flagella in intestinal colonizatio...

journal_title：Journal of bacteriology

authors： Sterzenbach T,Bartonickova L,Behrens W,Brenneke B,Schulze J,Kops F,Chin EY,Katzowitsch E,Schauer DB,Fox JG,Suerbaum S,Josenhans C

更新日期：2008-10-01 00:00:00

abstract：:In Bacillus subtilis the trpX locus specifies a glutamine-binding protein designated subunit X, which forms a complex with subunit E to constitute the anthranilate synthase enzyme aggregate (EX) and subunit A to constitute the p-aminobenzoate synthase enzyme aggregate (AX). Subunit X confers upon these enzyme complexe...

journal_title：Journal of bacteriology

authors： Kane JF

更新日期：1977-11-01 00:00:00

abstract：:Flavobacterium johnsoniae cells move rapidly over surfaces by gliding motility. Gliding results from the movement of adhesins such as SprB and RemA along the cell surface. These adhesins are delivered to the cell surface by a Bacteroidetes-specific secretion system referred to as the type IX secretion system (T9SS). G...

journal_title：Journal of bacteriology

authors： Shrivastava A,Johnston JJ,van Baaren JM,McBride MJ

更新日期：2013-07-01 00:00:00

abstract：:Glycerol is a primary energy source for heterotrophic haloarchaea and a major component of "salty" biodiesel waste. Glycerol is catabolized solely by glycerol kinase (encoded by glpK) to glycerol-3-phosphate (G3P) in Haloferax volcanii. Here we characterized the next critical step of this metabolic pathway: the conver...

journal_title：Journal of bacteriology

authors： Rawls KS,Martin JH,Maupin-Furlow JA

更新日期：2011-09-01 00:00:00

abstract：:The stator of the sodium-driven flagellar motor of Vibrio alginolyticus is a membrane protein complex composed of four PomA and two PomB subunits. PomB has a peptidoglycan-binding motif in the C-terminal region. In this study, four kinds of PomB deletions in the C terminus were constructed. None of the deletion protei...

journal_title：Journal of bacteriology

authors： Yakushi T,Hattori N,Homma M

更新日期：2005-01-01 00:00:00

abstract：:We isolated five new temperature-sensitive alleles of the essential cell division gene ftsZ in Escherichia coli, using P1-mediated, localized mutagenesis. The five resulting single amino acid changes (Gly109-->Ser109 for ftsZ6460, Ala129-->Thr129 for ftsZ972, Val157-->Met157 for ftsZ2066, Pro203-->Leu203 for ftsZ9124,...

journal_title：Journal of bacteriology

authors： Addinall SG,Small E,Whitaker D,Sturrock S,Donachie WD,Khattar MM

更新日期：2005-01-01 00:00:00

abstract：:Membrane vesicles capable of energy-dependent calcium uptake have been prepared from Bacillus megaterium cells in log-phase growth or when undergoing sporulation. The uptake is dependent on the calcium concentration and appears saturable in vesicles from cells in log-phase growth. Both ascorbate and phenazine methosul...

journal_title：Journal of bacteriology

authors： Golub EE,Bronner F

更新日期：1974-09-01 00:00:00

abstract：:Pseudomonas aeruginosa isolates from chronic lung infections often overproduce alginate, giving rise to the mucoid phenotype. Isolation of mucoid strains from chronic lung infections correlates with a poor patient outcome. The most common mutation that causes the mucoid phenotype is called mucA22 and results in a trun...

journal_title：Journal of bacteriology

authors： Cross AR,Raghuram V,Wang Z,Dey D,Goldberg JB

更新日期：2020-09-23 00:00:00