Gene transfer in magnetic bacteria: transposon mutagenesis and cloning of genomic DNA fragments required for magnetosome synthesis.

abstract：:The chromatophores of Chromatium vinosum, as well as six other photosynthetic bacteria, contained two or more proteins which were insoluble when heated in the presence of sodium dodecyl sulfate (SDS) and 2-mercaptoethanol (beta-ME). When the chromatophores were dissolved at room temperature in SDS-beta-ME, these prote...

journal_title：Journal of bacteriology

authors： Hui KM,Hurlbert RE

更新日期：1979-04-01 00:00:00

abstract：:A new insertion sequence, IS1634, has been identified in Mycoplasma mycoides subsp. mycoides small-colony type (SC). IS1634 shows structural and functional similarities to IS1549 of Mycobacterium smegmatis and with it seems to form a new class or family of insertion sequences. IS1634 has a size of 1,872 bp, including ...

journal_title：Journal of bacteriology

authors： Vilei EM,Nicolet J,Frey J

更新日期：1999-02-01 00:00:00

abstract：:Lipopolysaccharide (LPS) is an essential component of the outer membrane (OM) in most gram-negative bacteria, and its structure and biosynthetic pathway are well known. Nevertheless, the mechanisms of transport and assembly of this molecule at the cell surface are poorly understood. The inner membrane (IM) transport p...

journal_title：Journal of bacteriology

authors： Sperandeo P,Lau FK,Carpentieri A,De Castro C,Molinaro A,Dehò G,Silhavy TJ,Polissi A

更新日期：2008-07-01 00:00:00

abstract：:An enzyme which liberates Pi from myo-inositol hexaphosphate (phytic acid) was shown to be present in culture filtrates of Bacillus subtilis. It was purified until it was homogeneous by ultracentrifugation, but it still showed two isozymes on polyacrylamide gel electrophoresis. The enzyme differed from other previousl...

journal_title：Journal of bacteriology

authors： Powar VK,Jagannathan V

更新日期：1982-09-01 00:00:00

abstract：:The 50S ribosomal subunits obtained from exponential-phase and valine-limited cultures of Streptococcus faecalis 9790 differed in protein content. Approximately 35% of the valine-limited subunits showed protein losses when analyzed by CsCl density centrifugation after formaldehyde fixation. Results of density gradient...

journal_title：Journal of bacteriology

authors： Ziegler RJ,Daneo-Moore L

更新日期：1971-01-01 00:00:00

abstract：:The transforming activity of fractionated complementary strands of Diplococcus pneumoniae deoxyribonucleic acid (DNA) bands at the position of fully denatured DNA in CsCl at pH 11.0, and is completely (> 99.8%) destroyed by digestion with exonuclease-I. These results prove that pure single strands transform the normal...

journal_title：Journal of bacteriology

authors： Miao R,Guild WR

更新日期：1970-02-01 00:00:00

abstract：:Disruption of gene HI0894 or HI0895 in Haemophilus influenzae Rd, homologs of Escherichia coli acrAB multidrug efflux genes, caused hypersusceptibility to erythromycin, rifampin, novobiocin, and dyes such as ethidium bromide and crystal violet and increased accumulation of radioactive erythromycin, showing that these ...

journal_title：Journal of bacteriology

authors： Sánchez L,Pan W,Viñas M,Nikaido H

更新日期：1997-11-01 00:00:00

abstract：:Group B streptococcus (GBS) pili may enhance colonization and infection by mediating bacterial adhesion to host cells, invasion across endothelial and epithelial barriers, and resistance to bacterial ingestion and killing by host phagocytes. However, it remains unclear how pilus expression is regulated and how modulat...

journal_title：Journal of bacteriology

authors： Jiang S,Park SE,Yadav P,Paoletti LC,Wessels MR

更新日期：2012-05-01 00:00:00

abstract：:A-factor (2-isocapryloyl-3R-hydroxymethyl-gamma-butyrolactone) is essential for the initiation of aerial mycelium formation in Streptomyces griseus. amfR is one of the genes which, when cloned on a low-copy-number plasmid, suppresses the aerial mycelium-negative phenotype of an A-factor-deficient mutant of S. griseus....

journal_title：Journal of bacteriology

authors： Ueda K,Hsheh CW,Tosaki T,Shinkawa H,Beppu T,Horinouchi S

更新日期：1998-10-01 00:00:00

abstract：:Mycoplasma pneumoniae exhibits a novel form of gliding motility that is mediated by the terminal organelle, a differentiated polar structure. Given that genes known to be involved in gliding in other organisms are absent in M. pneumoniae, random transposon mutagenesis was employed to generate mutants with gliding-defi...

journal_title：Journal of bacteriology

authors： Page CA,Krause DC

更新日期：2013-04-01 00:00:00

abstract：:Slein, Milton W. (Fort Detrick, Frederick, Md.) and Gerald F. Logan, Jr. Mechanism of action of the toxin of Bacillus anthracis. II. Alkaline phosphatasemia produced by culture filtrates of various bacilli. J. Bacteriol. 83:359-369. 1962.-A factor which produces hyperphosphatasemia after intravenous injection into ani...

journal_title：Journal of bacteriology

authors： Slein MW,Logan GF

更新日期：1962-02-01 00:00:00

abstract：:Aspergillus fumigatus, an important opportunistic pathogen which commonly affects neutropenic patients, produces conidia with a bluish-green color. We identified a gene, alb1, which is required for conidial pigmentation. The alb1 gene encodes a putative polyketide synthase, and disruption of alb1 resulted in an albino...

journal_title：Journal of bacteriology

authors： Tsai HF,Chang YC,Washburn RG,Wheeler MH,Kwon-Chung KJ

更新日期：1998-06-01 00:00:00

abstract：:McFadden, B. A. (Washington State University, Pullman), and H. R. Homann. Characteristics and intermediates of short-term C(14)O(2) incorporation during ribose oxidation by Hydrogenomonas facilis. J. Bacteriol. 89:839-847. 1965.-Ribose-grown cells of Hydrogenomonas facilis, which had been suspended in growth medium an...

journal_title：Journal of bacteriology

authors： MCFADDEN BA,HOMANN HR

更新日期：1965-03-01 00:00:00

abstract：:Integration host factor (IHF) is a small, basic protein that is needed for efficient recombination of bacteriophage lambda, as well as for other host and viral functions. We have constructed strains in which the two subunits of IHF, encoded by the himA and hip genes of Escherichia coli, are expressed under the control...

journal_title：Journal of bacteriology

authors： Nash HA,Robertson CA,Flamm E,Weisberg RA,Miller HI

更新日期：1987-09-01 00:00:00

abstract：:Using representational difference analysis, we isolated novel meningococcal restriction-modification (R-M) systems. NmeBI, which is a homologue of the R-M system HgaI of Pasteurella volantium, was present in meningococci of the ET-5 complex and of lineage III. NmeAI was found in serogroup A, ET-37 complex, and cluster...

journal_title：Journal of bacteriology

authors： Claus H,Friedrich A,Frosch M,Vogel U

更新日期：2000-03-01 00:00:00

abstract：:Gill lamellae from the young fruiting bodies of the basidiomycete Coprinus micaceus possess cytoplasmic particles with cytochemically demonstrable acid phosphatase activity; they are presumed to be lysosomes. ...

journal_title：Journal of bacteriology

authors： Nehemiah JL

更新日期：1973-07-01 00:00:00

abstract：:We have previously used the bacteriophage transposon Mu d1 (which encodes the lacZY structural genes but without their promoter) to construct strains that have lacZY fused to phosphate-regulated promoters in Escherichia coli K-12. Among 18 identified phosphate-starvation-inducible (psi) genes, three (the phoA and two ...

journal_title：Journal of bacteriology

authors： Wanner BL,Wieder S,McSharry R

更新日期：1981-04-01 00:00:00

abstract：:Expression plasmids containing various portions of araFGH operon sequences were assayed for their ability to facilitate the high-affinity L-arabinose transport process in a strain lacking the chromosomal copy of this operon. Accumulation studies demonstrated that the specific induction of all three operon coding seque...

journal_title：Journal of bacteriology

authors： Horazdovsky BF,Hogg RW

更新日期：1989-06-01 00:00:00

abstract：:The steps in the biosynthetic transformation of GTP to 7,8-dihydro-D-erythro-neopterin (H2neopterin), the precursor to the modified folates found in the methanogenic archaea, has been elucidated for the first time in two members of the domain Archaea. In Methanococcus thermophila and Methanobacterium thermoautotrophic...

journal_title：Journal of bacteriology

authors： Howell DM,White RH

更新日期：1997-08-01 00:00:00

abstract：:The lambda S lysis gene was cloned into a Saccharomyces cerevisiae expression vector under GAL1 control. Induction with galactose in S. cerevisiae terminated cell growth and prevented colony formation. Several membrane proteins immunoreactive with anti-S antibody accumulated in the membranes, indicating that sodium do...

journal_title：Journal of bacteriology

authors： Garrett J,Bruno C,Young R

更新日期：1990-12-01 00:00:00

abstract：:Antibiotic-resistant Acinetobacter baumannii causes infections that are extremely difficult to treat. A significant role in these resistance profiles is attributed to multidrug efflux pumps, especially those belonging to the resistance-nodulation-cell division (RND) superfamily of transporters. In this study, we analy...

journal_title：Journal of bacteriology

authors： Leus IV,Weeks JW,Bonifay V,Smith L,Richardson S,Zgurskaya HI

更新日期：2018-06-11 00:00:00

abstract：:Decreased intracellular levels of FtsH, a membrane-bound ATPase, led to retardation of growth and protein export, as well as to an abnormal translocation of alkaline phosphatase that had been attached to a cytoplasmic domain of a multispanning membrane protein, SecY. The last phenotype is designated Std (stop transfer...

journal_title：Journal of bacteriology

authors： Shirai Y,Akiyama Y,Ito K

更新日期：1996-02-01 00:00:00

abstract：:The copy number and stability of artificial 2-micron circle-based plasmids have been accurately measured in [Cir+] and [Cir0] strains of Saccharomyces cerevisiae. We conclude that (i) instability and copy number vary greatly from plasmid to plasmid; (ii) instability and copy number are negatively correlated--that is, ...

journal_title：Journal of bacteriology

authors： Futcher AB,Cox BS

更新日期：1984-01-01 00:00:00

abstract：:Methanol dehydrogenase, the enzyme that oxidizes methanol to formaldehyde in gram-negative methylotrophs, contains the prosthetic group pyrroloquinoline quinone (PQQ). To begin to analyze how the synthesis of PQQ is coordinated with the production of other methanol dehydrogenase components, the transcription of one of...

journal_title：Journal of bacteriology

authors： Ramamoorthi R,Lidstrom ME

更新日期：1995-01-01 00:00:00

abstract：:The product of the papD gene of uropathogenic Escherichia coli is required for the biogenesis of digalactoside-binding P pili. Mutations within papD result in complete degradation of the major pilus subunit, PapA, and of the pilinlike proteins PapE and PapF and also cause partial breakdown of the PapG adhesin. The pap...

journal_title：Journal of bacteriology

authors： Lindberg F,Tennent JM,Hultgren SJ,Lund B,Normark S

更新日期：1989-11-01 00:00:00

abstract：:Nishida, Shoki (Kanazawa University, Kanazawa, Japan), and Gizo Nakagawara. Relationship between toxigenicity and sporulating potency of Clostridium novyi. J. Bacteriol. 89:993-995. 1965.-The less toxigenic the strains, the stronger was the sporulating potency of Clostridium novyi strains isolated. This was confirmed ...

journal_title：Journal of bacteriology

authors： NISHIDA S,NAKAGAWARA G

更新日期：1965-04-01 00:00:00

abstract：:Upon fractionating Saccharomyces cerevisiae asynchronous cultures by sucrose density gradient centrifugation in a zonal rotor and examining the exo-1,3-beta-glucanase and deoxyribonucleic acid content of the cells, a periodic step increase in the activity of this enzyme was observed, indicating a discontinuous pattern...

journal_title：Journal of bacteriology

authors： del Rey F,Santos T,García-Acha I,Nombela C

更新日期：1979-09-01 00:00:00

abstract：:The Escherichia coli pur regulon repressor protein was overproduced in a phage T7 expression system. The overexpressed repressor constituted approximately 35% of the soluble cellular protein. Pur repressor was purified to near homogeneity by two chromatographic steps. Hypoxanthine or guanine was required for binding o...

journal_title：Journal of bacteriology

authors： Rolfes RJ,Zalkin H

更新日期：1990-10-01 00:00:00

abstract：:The metabolism of mRNA from the lactose (lac) operon of Escherichia coli has been studied in ribonuclease (RNase) III-deficient strains (rnc-105). The induction lag for beta-galactosidase from the first gene was twice as long, and enzyme synthesis was reduced 10-fold in one such mutant compared with its isogenic rnc+ ...

journal_title：Journal of bacteriology

authors： Talkad V,Achord D,Kennell D

更新日期：1978-08-01 00:00:00

abstract：:Previous microarray studies have suggested that an indirect mechanism of Fur regulation may be present in meningococcus at the posttranscriptional level through a small regulatory RNA (sRNA) system analogous to that of Escherichia coli and Pseudomonas aeruginosa. Recently, a Fur-regulated sRNA, NrrF, was identified th...

journal_title：Journal of bacteriology

authors： Metruccio MM,Fantappiè L,Serruto D,Muzzi A,Roncarati D,Donati C,Scarlato V,Delany I

更新日期：2009-02-01 00:00:00