Abstract:
:The Haemophilus influenzae Rd rec-1+ gene was cloned from a partial chromosomal digest into a plasmid vector as a 20-kilobase-pair (kbp) BstEII fragment and then subcloned. The smallest subclone with rec-1+ activity carried a 3.1-kbp EcoRI fragment. The identity of the rec-I gene in these clones was confirmed by transforming an Rd strain carrying a leaky rec-1 mutation (recA4) to resistance to methyl methanesulfonate (MMS) by using whole or digested plasmids. It was demonstrated that the Rec+ phenotype of the MMSr transformants was linked to the strA, novAB, and mmsA loci, as expected if the recA4 allele had been replaced by rec-1+. In growing cultures (rec-1 or rec+), all rec-1+-carrying plasmids induced near-maximal levels of transformability when their hosts reached stationary phase; these levels are 100 to 1,000 times higher than the values seen with strains not carrying a Rec plasmid. Transfer of the 3.1-kbp subclone was greatly reduced compared with transfer of similarly sized vector plasmids, and the resulting transformants grew slowly; this suggests an explanation of my failure to directly clone this fragment from chromosomal DNA digests. Transfer of a rec-1+ plasmid to a very poorly genetically transformable H. influenzae Rb strain resulted in greatly increased transformability. Transfer of such plasmids to a noncompetent H. influenzae Rc strain did not render this strain competent. It is suggested that transformability of Rd and Rb strains is limited by rec-1 expression but that the noncompetence of Rc has some other basis.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Stuy JHdoi
10.1128/jb.171.8.4395-4401.1989subject
Has Abstractpub_date
1989-08-01 00:00:00pages
4395-401issue
8eissn
0021-9193issn
1098-5530journal_volume
171pub_type
杂志文章abstract::Acidithiobacillus caldus is an extremely acidophilic, moderately thermophilic, chemolithoautotrophic gammaproteobacterium that derives energy from the oxidation of sulfur and reduced inorganic sulfur compounds. Here we present the draft genome sequence of Acidithiobacillus caldus ATCC 51756 (the type strain of the spe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00843-09
更新日期:2009-09-01 00:00:00
abstract::The alpha (62,000-dalton) and beta (49,000-dalton) subunits of Methanosarcina barkeri ATPase were purified to homogeneity. The subunits and ATPase complex were trypsinized in the presence of various nucleotides. ATP and ADP changed the trypsin sensitivity of the alpha subunit in the complex and isolated forms, suggest...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.12.5960-5962.1988
更新日期:1988-12-01 00:00:00
abstract::Between 59 and 60 min on the Escherichia coli genetic map, there is a highly conserved sequence of 29 base pairs, containing an inverted repeat of seven base pairs that appears 14 times, 32 or 33 base pairs apart, downstream of the iap gene coding region. About 24 kilobase pairs downstream of the 14 repeats, a similar...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.6.3553-3556.1989
更新日期:1989-06-01 00:00:00
abstract::ibeA is a virulence factor found in some extraintestinal pathogenic Escherichia coli (ExPEC) strains from the B2 phylogenetic group and particularly in newborn meningitic and avian pathogenic strains. It was shown to be involved in the invasion process of the newborn meningitic strain RS218. In a previous work, we sho...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00089-12
更新日期:2012-09-01 00:00:00
abstract::Our whole-genome microarray studies of Neisseria meningitidis MC58 previously identified a set of 153 genes whose transcription was activated during growth in iron. In this study, Fur-mediated regulation of the iron-activated nspA gene was confirmed, whereas iron-activated regulation of the secY gene was demonstrated ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01638-06
更新日期:2007-01-01 00:00:00
abstract::We constructed a conditional mutant of pbpA in which transcription of the gene was placed under the control of an IPTG (isopropyl-beta-D-thiogalactopyranoside)-inducible promoter in order to explore the role of PBP1 in growth, cell wall structure, and cell division. A methicillin-resistant strain and an isogenic methi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00044-07
更新日期:2007-05-01 00:00:00
abstract::The allosteric pattern of control for 3-deoxy-d-arabino-heptulosonate 7-phosphate (DAHP) synthetase has previously been shown to be strongly conserved among the member species of a given genus in bacteria. The implications of this finding as a procedural tool of bacterial phylogeny were pursued by a study of two organ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.101.3.763-769.1970
更新日期:1970-03-01 00:00:00
abstract::We studied the roles of Streptococcus thermophilus phosphogalactosyltransferase (EpsE) (the priming enzyme), tyrosine kinase (EpsD), phosphatase (EpsB), and a membrane-associated protein with no known biochemical function (EpsC) in exopolysaccharide (EPS) synthesis. These proteins are well-conserved among bacteria and...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01122-06
更新日期:2007-02-01 00:00:00
abstract::The skin represents an important barrier for pathogens and is known to produce fatty acids that are toxic toward gram-positive bacteria. A screen of fatty acids as growth inhibitors of Staphylococcus aureus revealed structure-specific antibacterial activity. Fatty acids like oleate (18:1Δ9) were nontoxic, whereas palm...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00743-12
更新日期:2012-10-01 00:00:00
abstract::As with other inducible enzymes, the induced synthesis of l-arabinose isomerase (l-arabinose ketol isomerase, EC 5.3.1.4) in Salmonella typhimurium is subject to catabolite repression. Of the three catabolite repressors tested, glucose produces maximum repression. Analogues of catabolite repressors like 2-deoxy-d-gluc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.106.1.107-112.1971
更新日期:1971-04-01 00:00:00
abstract::Glucosylated deoxyribonucleic acid (DNA) from phages T4 and T6 competes poorly with homologous DNA causing only a slight decrease of transformation in Group H Streptococcus strain Challis. Other types of heterologous DNAs (Micrococcus luteus, Clostridium perfringens, Escherichia coli, calf thymus and non-glucosylated ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.3.1621-1623.1975
更新日期:1975-12-01 00:00:00
abstract::In a previous paper it was shown that cocultivation of Rhizobium leguminosarum with the plant Vicia sativa subsp. nigra on solid medium causes a changed mode of growth of the plant roots, resulting in thick and short roots (Tsr). The Sym plasmid present in the bacterium appeared to be essential for causing Tsr (A. A. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.165.2.517-522.1986
更新日期:1986-02-01 00:00:00
abstract::Genetic transformation of Sulfolobus acidocaldarius by a multiply marked pyrE gene provided a high-resolution assay of homologous recombination in a hyperthermophilic archaeon. Analysis of 100 Pyr(+) transformants revealed that this recombination system could transfer each of 23 nonselected base pair substitutions to ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00447-10
更新日期:2010-09-01 00:00:00
abstract::Transmission electron microscopy was used to examine active ribosomal ribonucleic acid (rRNA) genes in two strains of Escherichia coli: N2077, deficient in the enzyme responsible for proper cleavage of the 16S sequence from the elongating nascent rRNA transcript; and N2076, functional in ribonuclease (RNase) III activ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.132.2.718-722.1977
更新日期:1977-11-01 00:00:00
abstract::The lining of the maltodextrin-specific maltoporin (LamB) channel exhibits a string of aromatic residues, the greasy slide, part of which has been shown previously by crystallography to be involved in substrate binding. To probe the functional role of the greasy slide, alanine scanning mutagenesis has been performed o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.11.2994-2999.2002
更新日期:2002-06-01 00:00:00
abstract::The vir-type IV secretion system of Agrobacterium is assembled from 12 proteins encoded by the virB operon and virD4. VirB1 is one of the least-studied proteins encoded by the virB operon. Its N terminus is a lytic transglycosylase. The C-terminal third of the protein, VirB1*, is cleaved from VirB1 and secreted to the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00480-07
更新日期:2007-09-01 00:00:00
abstract::The major conjugative transfer gene cluster of staphylococcal plasmid pGO1 (trs) consists of 13 open reading frames (trsA to trsM) transcribed from one DNA strand and a single 189-bp open reading frame (trsN) within the first 348 bp of trs that is transcribed divergently. Promoter regions for trsN and trsA partially o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.12.3445-3454.1994
更新日期:1994-06-01 00:00:00
abstract::A third nuclear protein-coding gene termed PET122 has been shown to be required for a post-transcriptional step in expression of the mitochondrial COX3 gene is Saccharomyces cerevisiae. pet122 mutants fail to produce cytochrome c oxidase subunit III, which is the polypeptide product of the COX3 gene, but produce norma...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.3.1399-1402.1988
更新日期:1988-03-01 00:00:00
abstract::Several mutants of Bacillus subtilis deficient in catalase synthesis generated by nitrosoguanidine mutagenesis have been used to map a locus affecting catalase activity. Two- and three-factor bacteriophage PBS1 transductional crosses were used to locate the locus, named katA, between recH and thiA with 98% linkage to ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.12.5848-5851.1987
更新日期:1987-12-01 00:00:00
abstract::The sets of compounds that can support growth of an organism are defined by the presence of transporters and metabolic pathways that convert nutrient sources into cellular components and energy for growth. A collection of known nutrient sources can therefore serve both as an impetus for investigating new metabolic pat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01209-13
更新日期:2014-03-01 00:00:00
abstract::Davies, Helen C. (University of Pennsylvania, Philadelphia), Fred Karush, and Joanne H. Rudd. Effect of amino acids on steady-state growth of a group A hemolytic streptococcus. J. Bacteriol. 89:421-427. 1965.-A study has been made of amino acid utilization by a strain of type 4, group A streptococcus growing under ste...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1965-02-01 00:00:00
abstract::Picolinic acid (PA) is a natural toxic pyridine derivative. Microorganisms can degrade and utilize PA for growth. However, the full catabolic pathway of PA and its physiological and genetic foundation remain unknown. In this study, we identified a gene cluster, designated picRCEDFB4B3B2B1A1A2A3, responsible for the de...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00077-19
更新日期:2019-07-24 00:00:00
abstract::The mutations of eight chemotaxis-deficient strains of Salmonella typhimurium, including five new mutants in strain LT2, were mapped by P22 transduction in relation to various fla mot deletions in S. abortus-equi. Seven recessive che mutations mapped between motB and flaC: three, all nontumbling, the che region I, adj...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.128.3.754-765.1976
更新日期:1976-12-01 00:00:00
abstract::We previously described a cell surface anionic polysaccharide (APS) in Porphyromonas gingivalis that is required for cell integrity and serum resistance. APS is a phosphorylated branched mannan that shares a common epitope with posttranslational additions to some of the Arg-gingipains. This study aimed to determine th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01868-07
更新日期:2008-04-01 00:00:00
abstract::The global transcriptional regulator Spx of Bacillus subtilis is controlled at several levels of the gene expression process. It is maintained at low concentrations during unperturbed growth by the ATP-dependent protease ClpXP. Under disulfide stress, Spx concentration increases due in part to a reduction in ClpXP-cat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01289-08
更新日期:2009-02-01 00:00:00
abstract::The RNA polymerase sigma subunit, sigmaH, of Bacillus subtilis is required for the transcription of genes that are induced in late-growth cultures at high cell density, including genes that function in sporulation. The expression of sigmaH-controlled genes is repressed when nutrient broth sporulation medium (Difco spo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.21.6778-6787.1997
更新日期:1997-11-01 00:00:00
abstract::Escherichia coli O111a is a thermosensitive strain which, when grown at 40 C, accumulates large quantities of intracellular membranes. The ultrastructure of these membranes in cells which have been chemically fixed, embedded, and examined as thin sections has been compared with that of membranes in cells negatively st...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.113.1.433-444.1973
更新日期:1973-01-01 00:00:00
abstract::The ribosomal proteins from nine species of prokaryotes have been compared by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The stained gels were scanned spectrophotometrically, the weight and number average molecular weights were calculated, and the detailed distribution of the protein...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.111.2.474-480.1972
更新日期:1972-08-01 00:00:00
abstract::Two nifA-like genes, designated anfA and vnfA, have been identified in Azotobacter vinelandii. The anfA gene is located upstream from the nitrogenase-3 structural gene cluster (anfHDGK) and is preceded by a sequence that is potentially part of a ntrA-dependent promoter. The product of anfA appears to be required for e...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.6.3258-3267.1989
更新日期:1989-06-01 00:00:00
abstract::A heat-stable, particulate, lipopolysaccharide-protein antigenic complex has been isolated from a virulent, encapsulated strain of Pasteurella multocida by extraction with cold, formalinized saline, and centrifugation at 105,000 x g. The original bacterial culture had been obtained from a bison that died of hemorrhagi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.1.7-14.1967
更新日期:1967-01-01 00:00:00