Relationship of a wall-associated enzyme with specific layers of the cell wall of a gram-negative bacterium.

abstract：:The mechanisms involved in the virulence of Yersinia pestis, the plague pathogen, are not fully understood. In previous research, we found that a Y. pestis mutant lacking the HicB3 (YPO3369) putative orphan antitoxin was attenuated for virulence in a murine model of bubonic plague. Toxin-antitoxin systems (TASs) are w...

journal_title：Journal of bacteriology

authors： Bibi-Triki S,Li de la Sierra-Gallay I,Lazar N,Leroy A,Van Tilbeurgh H,Sebbane F,Pradel E

更新日期：2014-11-01 00:00:00

abstract：:NADPH is an intermediate in the oxidation of organic compounds coupled to Fe(III) reduction in Geobacter species, but Fe(III) reduction with NADPH as the electron donor has not been studied in these organisms. Crude extracts of Geobacter sulfurreducens catalyzed the NADPH-dependent reduction of Fe(III)-nitrilotriaceti...

journal_title：Journal of bacteriology

authors： Kaufmann F,Lovley DR

更新日期：2001-08-01 00:00:00

abstract：:ermC specifies an rRNA methyltransferase that confers resistance to erythromycin. The expression of this determinant is induced by the addition of erythromycin. The induction mechanism has been shown to operate posttranscriptionally, and its mechanism has been elucidated. We now show that synthesis of the ermC gene pr...

journal_title：Journal of bacteriology

authors： Denoya CD,Bechhofer DH,Dubnau D

更新日期：1986-12-01 00:00:00

abstract：:In previous work, we characterized the bases in an iteron of plasmid R6K that are important for the binding of pi protein monomers and dimers. Here we investigate the following six amino acids of pi, encoded by pir, hypothesized to be important for DNA contact: Ser71, Try74, Gly131, Gly211, Arg225, and Arg254. ...

journal_title：Journal of bacteriology

authors： Kunnimalaiyaan S,Rakowski SA,Filutowicz M

更新日期：2007-07-01 00:00:00

abstract：:Escherichia coli O111a is a thermosensitive strain which, when grown at 40 C, accumulates large quantities of intracellular membranes. The ultrastructure of these membranes in cells which have been chemically fixed, embedded, and examined as thin sections has been compared with that of membranes in cells negatively st...

journal_title：Journal of bacteriology

authors： Weigand RA,Holt SC,Shively JM,Decker GL,Greenawalt JW

更新日期：1973-01-01 00:00:00

abstract：:With a DNA fragment from within the region encoding the transport functions for K1 production as a hybridization probe in Southern blot experiments, homologous DNA sequences were detected in the DNA from Escherichia coli strains producing K5, K7, K92, and K100 capsular polysaccharides. No homology with the laboratory ...

journal_title：Journal of bacteriology

authors： Roberts I,Mountford R,High N,Bitter-Suermann D,Jann K,Timmis K,Boulnois G

更新日期：1986-12-01 00:00:00

abstract：:Wild-type Escherichia coli cannot grow on L-1,2-propanediol; mutants that can do so have increased basal activity of an NAD-linked L-1,2-propanediol oxidoreductase. This enzyme belongs to the L-fucose system and functions normally as L-lactaldehyde reductase during fermentation of the methylpentose. In wild-type cells...

journal_title：Journal of bacteriology

authors： Chen YM,Chakrabarti T,Lin EC

更新日期：1984-08-01 00:00:00

abstract：:The fruK gene encoding fructose-1-phosphate kinase (FruK), located within the fructose (fru)-catabolic operon of Rhodobacter capsulatus, was sequenced. FruK of R. capsulatus (316 amino acids; molecular weight = 31,232) is the same size as and is homologous to FruK of Escherichia coli, phosphofructokinase B (PfkB) of E...

journal_title：Journal of bacteriology

authors： Wu LF,Reizer A,Reizer J,Cai B,Tomich JM,Saier MH Jr

更新日期：1991-05-01 00:00:00

abstract：:FK-506-binding proteins (FKBPs), which in T cells are supposed to mediate the immunosuppressive effects of the compounds FK-506 and rapamycin, have been isolated from Streptomyces chrysomallus, S. hygroscopicus subsp. ascomyceticus, and S. hygroscopicus. The latter two strains are producers of ascomycin (the ethyl ana...

journal_title：Journal of bacteriology

authors： Pahl A,Keller U

更新日期：1992-09-01 00:00:00

abstract：:Rhodococcus sp. strain DK17 is capable of utilizing various derivatives of benzene and bicyclics containing both aromatic and alicyclic moieties as sole carbon and energy sources. Here, we present the 9,107,362-bp draft genome sequence of DK17 and its genomic analysis in comparison with other members of the genus Rhod...

journal_title：Journal of bacteriology

authors： Yoo M,Kim D,Choi KY,Chae JC,Zylstra GJ,Kim E

更新日期：2012-08-01 00:00:00

abstract：:The metD D-methionine transporter locus of Escherichia coli was identified as the abc-yaeE-yaeC cluster (now renamed metNIQ genes). The abc open reading frame is preceded by tandem MET boxes bracketed by the -10 and -35 boxes of a promoter. The expression driven by this promoter is controlled by the MetJ repressor and...

journal_title：Journal of bacteriology

authors： Gál J,Szvetnik A,Schnell R,Kálmán M

更新日期：2002-09-01 00:00:00

abstract：:Oligoribonuclease, an exoribonuclease specific for small oligoribonucleotides, was initially characterized 20 years ago (S. K. Niyogi and A. K. Datta, J. Biol. Chem. 250:7307-7312, 1975) and shown to be different from RNase II and polynucleotide phosphorylase. Here we demonstrate, using mutant strains and purified enz...

journal_title：Journal of bacteriology

authors： Yu D,Deutscher MP

更新日期：1995-07-01 00:00:00

abstract：:Bacteria in biofilms often undergo active dispersal events and revert to a free-swimming, planktonic state to complete the biofilm life cycle. The signaling molecule nitric oxide (NO) was previously found to trigger biofilm dispersal in the opportunistic pathogen Pseudomonas aeruginosa at low, nontoxic concentrations ...

journal_title：Journal of bacteriology

authors： Barraud N,Schleheck D,Klebensberger J,Webb JS,Hassett DJ,Rice SA,Kjelleberg S

更新日期：2009-12-01 00:00:00

abstract：:Genes involved in allose utilization of Escherichia coli K-12 are organized in at least two operons, alsRBACE and alsI, located next to each other on the chromosome but divergently transcribed. Mutants defective in alsI (allose 6-phosphate isomerase gene) and alsE (allulose 6-phosphate epimerase gene) were Als(-). Tra...

journal_title：Journal of bacteriology

authors： Poulsen TS,Chang YY,Hove-Jensen B

更新日期：1999-11-01 00:00:00

abstract：:Bacillus subtilis grows under anaerobic conditions utilizing nitrate ammonification and various fermentative processes. The two-component regulatory system ResDE and the redox regulator Fnr are the currently known parts of the regulatory system for anaerobic adaptation. Mutation of the open reading frame ywiD located ...

journal_title：Journal of bacteriology

authors： Marino M,Ramos HC,Hoffmann T,Glaser P,Jahn D

更新日期：2001-12-01 00:00:00

abstract：:The Salmonella enterica serovar Typhi ompS2 gene codes for a 362-amino-acid outer membrane protein that contains motifs common to the porin superfamily. It is expressed at very low levels compared to the major OmpC and OmpF porins, as observed for S. enterica serovar Typhi OmpS1, Escherichia coli OmpN, and Klebsiella ...

journal_title：Journal of bacteriology

authors： Fernández-Mora M,Puente JL,Calva E

更新日期：2004-05-01 00:00:00

abstract：:As with other inducible enzymes, the induced synthesis of l-arabinose isomerase (l-arabinose ketol isomerase, EC 5.3.1.4) in Salmonella typhimurium is subject to catabolite repression. Of the three catabolite repressors tested, glucose produces maximum repression. Analogues of catabolite repressors like 2-deoxy-d-gluc...

journal_title：Journal of bacteriology

authors： Bhattacharya AK,Chakravorty M

更新日期：1971-04-01 00:00:00

abstract：:Expression of the Vibrio vulnificus metalloprotease gene, vvp, was turned up rapidly when bacterial growth reached the late log phase. A similar pattern of expression has been found in the metalloprotease gene of Vibrio cholerae, and this has been shown to be regulated by a Vibrio harveyi LuxR-like transcriptional act...

journal_title：Journal of bacteriology

authors： Shao CP,Hor LI

更新日期：2001-02-01 00:00:00

abstract：:The isolation of a new thermophilic bacterium, Thermus aquaticus gen. n. and sp. n., is described. Successful enrichment requires incubation at 70 to 75 C, and the use of nutrient media relatively dilute with respect to the organic components. Strains of T. aquaticus have been isolated from a variety of thermal spring...

journal_title：Journal of bacteriology

authors： Brock TD,Freeze H

更新日期：1969-04-01 00:00:00

abstract：:The KRE1 gene of Saccharomyces cerevisiae, sacKRE1, appears to be involved in the synthesis of cell wall beta-glucan. S. cerevisiae strains with mutations in the KRE1 gene produce a structurally altered cell wall (1----6)-beta-glucan, which results in resistance to K1 killer toxin. We isolated the canKRE1 gene from Ca...

journal_title：Journal of bacteriology

authors： Boone C,Sdicu A,Laroche M,Bussey H

更新日期：1991-11-01 00:00:00

abstract：:Nitroakridin 3582 (NA) formed complexes with native deoxyribonucleic acid (DNA) and with transfer ribonucleic acid (tRNA) species from Escherichia coli. Spectrophotometric titrations of NA with these nucleic acids produced numerical results from which nonlinear adsorption isotherms were derived. These curves indicated...

journal_title：Journal of bacteriology

authors： Wolfe AD,Cook TM,Hahn FE

更新日期：1971-12-01 00:00:00

abstract：:The yeast-phase cell cycle of Wangiella dermatitidis was studied using flow microfluorimetry and the deoxyribonucleic acid (DNA) synthesis inhibitor hydroxyurea (HU). Exposure of exponential-phase yeastlike cells to 0.1 M HU for 3 to 6 h resulted in the arrest of the cells in DNA synthesis and produced a nearly homoge...

journal_title：Journal of bacteriology

authors： Roberts RL,Szaniszlo PJ

更新日期：1980-11-01 00:00:00

abstract：:Growth of Escherichia coli in the presence of certain D-amino acids, such as D-methionine, results in the incorporation of the D-amino acid into macromolecular peptidoglycan and can be lethal at high concentrations. Previous studies suggested that incorporation was independent of the normal biosynthetic pathway. An en...

journal_title：Journal of bacteriology

authors： Caparrós M,Pisabarro AG,de Pedro MA

更新日期：1992-09-01 00:00:00

abstract：:The reoxidation of NADH generated in reactions within the mitochondrial matrix of Saccharomyces cerevisiae is catalyzed by an NADH dehydrogenase designated Ndi1p (C. A. M. Marres, S. de Vries, and L. A. Grivell, Eur. J. Biochem. 195:857-862, 1991). Gene disruption analysis was used to examine possible metabolic functi...

journal_title：Journal of bacteriology

authors： Small WC,McAlister-Henn L

更新日期：1998-08-01 00:00:00

abstract：:Products of the approximately 9.5-kb virB operon are proposed to direct the export of T-DNA/protein complexes across the Agrobacterium tumefaciens envelope en route to plant cells. The presence of conserved nucleoside triphosphate (NTP)-binding domains in VirB4 and VirB11 suggests that one or both proteins couple ener...

journal_title：Journal of bacteriology

authors： Berger BR,Christie PJ

更新日期：1993-03-01 00:00:00

abstract：:Haemophilus influenzae is a common gram-negative pathogen that initiates infection by colonizing the upper respiratory tract epithelium. In previous work, we reported the isolation of a locus involved in expression of short, thin surface fibrils by H. influenzae type b and presented evidence that surface fibrils promo...

journal_title：Journal of bacteriology

authors： St Geme JW 3rd,Cutter D,Barenkamp SJ

更新日期：1996-11-01 00:00:00

abstract：:Myxobacteria are predatory and are prolific producers of secondary metabolites. Here, we tested a hypothesized role that secondary metabolite antibiotics function as weapons in predation. To test this, a Myxococcus xanthus Δta1 mutant, blocked in antibiotic TA (myxovirescin) production, was constructed. This TA(-) mut...

journal_title：Journal of bacteriology

authors： Xiao Y,Wei X,Ebright R,Wall D

更新日期：2011-09-01 00:00:00

abstract：:Genomics research provides an unprecedented opportunity for us to probe into the pathogenicity and evolution of the world's most deadly pathogenic bacterium, Yersinia pestis, in minute detail. In our present work, extensive microarray analysis in conjunction with PCR validation revealed that there are considerable gen...

journal_title：Journal of bacteriology

authors： Zhou D,Han Y,Song Y,Tong Z,Wang J,Guo Z,Pei D,Pang X,Zhai J,Li M,Cui B,Qi Z,Jin L,Dai R,Du Z,Bao J,Zhang X,Yu J,Wang J,Huang P,Yang R

更新日期：2004-08-01 00:00:00

abstract：:Banerjee, Arun B. (University of Calcutta, Calcutta, India), and S. K. Bose. Biosynthesis of mycobacillin, a new antifungal peptide. II. Relation between streptomycin dependence and biosynthesis. J. Bacteriol. 87:1402-1405. 1964.-A streptomycin-dependent variant was isolated by a single-step mutation process from a st...

journal_title：Journal of bacteriology

authors： BANERJEE AB,BOSE SK

更新日期：1964-06-01 00:00:00

abstract：:The phs mutation of Escherichia coli has been suggested to affect the Na+/H+ antiport (D. Zilberstein, E. Padan, and S. Schuldiner, FEBS Lett. 168:327-330, 1980). We have recently shown that the mutation affects the rpoA gene and thus affects transcription. The extent of the pleiotropy of the phs mutation was investig...

journal_title：Journal of bacteriology

authors： Giffard PM,Rowland GC,Kroll RG,Stewart LM,Bakker EP,Booth IR

更新日期：1985-11-01 00:00:00