Conservation and variation of nucleotide sequences within related bacterial genomes: Escherichia coli strains.

Abstract:

:Changes in the patterns produced by annealing restriction endonuclease digests of bacterial genomes with probe deoxyribonucleic acids (DNAs) containing small portions of a bacterial genome provide sensitive indicator of the degree of nucleotide sequence relatedness that exists in localized regions of the genomes of closely related bacteria. We have used five probe DNAs to explore the relatedness of parts of the genomes of six laboratory Escherichi coli strains. A range in in the amount of variability in the positions of restriction enzyme cleavage sites in the selected portions of the genomes was found. Portions of the genome that are believed to be inacative were more variable than portions that contained functional genes: the sites in and near regions of homology to phage lambda DNA in the genome showed the greatest variability. These regions probably represent remnants of cryptic prophages. Variability was assessed pairwise among four of the E. coli strains and ranged from 5 to > 25% base pair substitutions in the lambda-related regions. In contrast, the endonuclease cleavage sites in the trp, tna, lac, thy regions, and one other as-yet-unidentified segment of the genome were more highly conserved. It seems likely that these sites lie in genetic locations that are subject to functional constraints.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Anilionis A,Riley M

doi

10.1128/JB.143.1.355-365.1980

subject

Has Abstract

pub_date

1980-07-01 00:00:00

pages

355-65

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

143

pub_type

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