The PmlI-PmlR quorum-sensing system in Burkholderia pseudomallei plays a key role in virulence and modulates production of the MprA protease.

Abstract:

:Burkholderia pseudomallei is the causative agent of melioidosis, an often fatal infection of humans and animals. The virulence of this pathogen is thought to depend on a number of secreted proteins, including the MprA metalloprotease. We observed that MprA is produced upon entry into the stationary phase, when the cell density is high, and this prompted us to study cell density-dependent regulation in B. pseudomallei. A search of the B. pseudomallei genome led to identification of a quorum-sensing system involving the LuxI-LuxR homologs PmlI-PmlR. PmlI directed the synthesis of an N-acylhomoserine lactone identified as N-decanoylhomoserine lactone. A B. pseudomallei pmlI mutant was significantly less virulent than the parental strain in a murine model of infection by the intraperitoneal, subcutaneous, and intranasal routes. Inactivation of pmlI resulted in overproduction of MprA at the onset of the stationary phase. A wild-type phenotype was restored following complementation with pmlI or addition of cell-free culture supernatant. In contrast, there was no significant difference between the virulence of a B. pseudomallei mprA mutant and the virulence of the wild-type strain. These results suggest that the PmlI-PmlR quorum-sensing system of B. pseudomallei is essential for full virulence in a mouse model and downregulates the production of MprA at a high cell density.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Valade E,Thibault FM,Gauthier YP,Palencia M,Popoff MY,Vidal DR

doi

10.1128/jb.186.8.2288-2294.2004

keywords:

subject

Has Abstract

pub_date

2004-04-01 00:00:00

pages

2288-94

issue

8

eissn

0021-9193

issn

1098-5530

journal_volume

186

pub_type

杂志文章
  • Physiological and biochemical characterization of the soluble formate dehydrogenase, a molybdoenzyme from Alcaligenes eutrophus.

    abstract::Organoautotrophic growth of Alcaligenes eutrophus on formate was dependent on the presence of molybdate in the medium. Supplementation of the medium with tungstate lead to growth cessation. Corresponding effects of these anions were observed for the activity of the soluble, NAD(+)-linked formate dehydrogenase (S-FDH; ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.15.4719-4728.1993

    authors: Friedebold J,Bowien B

    更新日期:1993-08-01 00:00:00

  • Tryptophan biosynthesis in Salmonella typhimurium: location in trpB of a genetic difference between strains LT2 and LT7.

    abstract::Salmonella typhimurium prototrophs carrying a trpR mutation synthesize tryptophan biosynthetic enzymes constitutively. When feedback inhibition of anthranilate synthetase but not 5'-phosphoribosylpyrophosphate phosphoribosyltransferase activity was by-passed by growing cells on media supplemented with anthranilic acid...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.123.3.878-887.1975

    authors: Stuttard C

    更新日期:1975-09-01 00:00:00

  • BIOLOGY OF BUDDING BACTERIA. 3. FINE STRUCTURE OF RHODOMICROBIUM AND HYPHOMICROBIUM SPP.

    abstract::Conti, S. F. (Dartmouth Medical School, Hanover, N.H.), and Peter Hirsch. Biology of budding bacteria. III. Fine structure of Rhodomicrobium and Hyphomicrobium spp. J. Bacteriol. 89:503-512. 1965.-The ultrastructure of 14 strains of hyphomicrobia, and of Rhodomicrobium vannielii, was investigated by means of electron ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.2.503-512.1965

    authors: CONTI SF,HIRSCH P

    更新日期:1965-02-01 00:00:00

  • Spore germination in strains of Dictyostelium discoideum and other members of the Dictyosteliaceae.

    abstract::Spores of all strains of Dictyostelium discoideum tested in this study germinated after a heat shock of 45 C for 30 min. Whereas the strains differed in their rates of germination, the rate for each strain was constant. A correlation existed between the rate of germination and the rate of vegetative growth when spores...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.5.1690-1695.1968

    authors: Cotter DA,Raper KB

    更新日期:1968-11-01 00:00:00

  • Ribosome synthesis in Rhodopseudomonas capsulata cells growing in continuous and intermittent light.

    abstract::The ribosome content was markedly reduced when illumination (at 28 C) was repeatedly interrupted; an opposite trend was observed with continuous light as the temperature was decreased. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.97.3.1518-1519.1969

    authors: Din GA,Gest H

    更新日期:1969-03-01 00:00:00

  • ArgR and AhrC are both required for regulation of arginine metabolism in Lactococcus lactis.

    abstract::The DNA binding proteins ArgR and AhrC are essential for regulation of arginine metabolism in Escherichia coli and Bacillus subtilis, respectively. A unique property of these regulators is that they form hexameric protein complexes, mediating repression of arginine biosynthetic pathways as well as activation of argini...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.4.1147-1157.2004

    authors: Larsen R,Buist G,Kuipers OP,Kok J

    更新日期:2004-02-01 00:00:00

  • RflM functions as a transcriptional repressor in the autogenous control of the Salmonella Flagellar master operon flhDC.

    abstract::Motility of bacteria like Salmonella enterica is a highly regulated process that responds to a variety of internal and external stimuli. A hierarchy of three promoter classes characterizes the Salmonella flagellar system, and the onset of flagellar gene expression depends on the oligomeric regulatory complex and class...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00728-13

    authors: Singer HM,Erhardt M,Hughes KT

    更新日期:2013-09-01 00:00:00

  • Bap, a Staphylococcus aureus surface protein involved in biofilm formation.

    abstract::Identification of new genes involved in biofilm formation is needed to understand the molecular basis of strain variation and the pathogenic mechanisms implicated in chronic staphylococcal infections. A biofilm-producing Staphylococcus aureus isolate was used to generate biofilm-negative transposon (Tn917) insertion m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.9.2888-2896.2001

    authors: Cucarella C,Solano C,Valle J,Amorena B,Lasa I,Penadés JR

    更新日期:2001-05-01 00:00:00

  • Rhodobacter capsulatus nifA1 promoter: high-GC -10 regions in high-GC bacteria and the basis for their transcription.

    abstract::It was previously shown that the Rhodobacter capsulatus NtrC enhancer-binding protein activates the R. capsulatus housekeeping RNA polymerase but not the Escherichia coli RNA polymerase at the nifA1 promoter. We have tested the hypothesis that this activity is due to the high G+C content of the -10 sequence. A compara...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.3.740-749.2004

    authors: Richard CL,Tandon A,Kranz RG

    更新日期:2004-02-01 00:00:00

  • Characterization of an exo-beta-D-glucosaminidase involved in a novel chitinolytic pathway from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1.

    abstract::We previously clarified that the chitinase from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 produces diacetylchitobiose (GlcNAc(2)) as an end product from chitin. Here we sought to identify enzymes in T. kodakaraensis that were involved in the further degradation of GlcNAc(2). Through a search of th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.17.5175-5181.2003

    authors: Tanaka T,Fukui T,Atomi H,Imanaka T

    更新日期:2003-09-01 00:00:00

  • Sequence of the pckA gene of Escherichia coli K-12: relevance to genetic and allosteric regulation and homology of E. coli phosphoenolpyruvate carboxykinase with the enzymes from Trypanosoma brucei and Saccharomyces cerevisiae.

    abstract::The sequence of the pckA gene coding for phosphoenolpyruvate carboxykinase in Escherichia coli K-12 and previous molecular weight determinations indicate that this allosteric enzyme is a monomer of Mr 51,316. The protein is homologous to ATP-dependent phosphoenolpyruvate carboxykinases from Trypanosoma brucei and Sacc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.12.7151-7156.1990

    authors: Medina V,Pontarollo R,Glaeske D,Tabel H,Goldie H

    更新日期:1990-12-01 00:00:00

  • F plasmid ccd mechanism in Escherichia coli.

    abstract::The ccd mechanism specified by the ccdA and ccdB genes of the mini-F plasmid determines fate of plasmid-free segregants in Escherichia coli (Jaffé et al., J. Bacteriol. 163:841-849, 1985). The killing function in plasmid-free segregants by the ccd mechanism did not affect cell growth of coexisting cells in the same cu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.166.1.100-104.1986

    authors: Hiraga S,Jaffé A,Ogura T,Mori H,Takahashi H

    更新日期:1986-04-01 00:00:00

  • Carrier-mediated transport of folate in a mutant of Pediococcus cerevisiae.

    abstract::A mutant strain of Pediococcus cerevisiae (P. cerevisiae/PteGlu) was isolated which grows on low-folate (PteGlu) concentrations (200 pg/ml). The growth response of the parent and mutant strains to folinate (5-CHO-H(4)PteGlu) was the same. The transport of (14)C-PteGlu by P. cerevisiae/PteGlu was temperature-dependent ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.114.2.485-490.1973

    authors: Mandelbaum-Shavit F,Grossowicz N

    更新日期:1973-05-01 00:00:00

  • Polynucleotide phosphorylase plays an important role in the generation of spontaneous mutations in Escherichia coli.

    abstract::Polynucleotide phosphorylase (PNP) plays a central role in RNA degradation, generating a pool of ribonucleoside diphosphates (rNDPs) that can be converted to deoxyribonucleoside diphosphates (dNDPs) by ribonucleotide reductase. We report here that spontaneous mutations resulting from replication errors, which are norm...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00962-12

    authors: Becket E,Tse L,Yung M,Cosico A,Miller JH

    更新日期:2012-10-01 00:00:00

  • Two-dimensional restriction analysis of the Bacillus subtilis genome: gene purification and ribosomal ribonucleic acid gene organization.

    abstract::With two-dimensional restriction enzyme analysis we have been able to cleave the Bacillus subtilis genome and resolve the resulting deoxyribonucleic acid (DNA) segments into discrete bands on agarose gels. A general procedure for gene purification has been developed by coupling multidimensional restriction analysis wi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.129.1.492-500.1977

    authors: Potter SS,Bott KF,Newbold JE

    更新日期:1977-01-01 00:00:00

  • Overproduction of SecA suppresses the export defect caused by a mutation in the gene encoding the Escherichia coli export chaperone secB.

    abstract::SecB is a cytosolic protein required for rapid and efficient export of particular periplasmic and outer membrane proteins in Escherichia coli. SecB promotes export by stabilizing newly synthesized precursor proteins in a nonnative conformation and by targeting the precursors to the inner membrane. Biochemical studies ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.10.3010-3017.1999

    authors: Cook HA,Kumamoto CA

    更新日期:1999-05-01 00:00:00

  • Cloning of the C-terminal cytoplasmic fragment of the tar protein and effects of the fragment on chemotaxis of Escherichia coli.

    abstract::A gene encoding only the C-terminal portion of the receptor-transducer protein Tar of Escherichia coli was constructed. The gene product was detected and localized in the cytoplasmic fraction of the cell by immunoblotting with anti-Tar antibodies. The C-terminal fragments from wild-type and mutant tar genes were chara...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.6.2521-2526.1988

    authors: Oosawa K,Mutoh N,Simon MI

    更新日期:1988-06-01 00:00:00

  • Cell-Biological Studies of Osmotic Shock Response in Streptomyces spp.

    abstract::Most bacteria are likely to face osmotic challenges, but there is yet much to learn about how such environmental changes affect the architecture of bacterial cells. Here, we report a cell-biological study in model organisms of the genus Streptomyces, which are actinobacteria that grow in a highly polarized fashion to ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00465-16

    authors: Fuchino K,Flärdh K,Dyson P,Ausmees N

    更新日期:2016-12-13 00:00:00

  • Inhibition of Escherichia coli acetyl coenzyme A carboxylase by acyl-acyl carrier protein.

    abstract::Escherichia coli acetyl coenzyme A carboxylase (ACC), the first enzyme of the fatty acid biosynthetic pathway, is inhibited by acylated derivatives of acyl carrier protein (ACP). ACP lacking an acyl moiety does not inhibit ACC. Acylated derivatives of ACP having chain lengths of 6 to 20 carbon atoms were similarly inh...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.4.1499-1503.2001

    authors: Davis MS,Cronan JE Jr

    更新日期:2001-02-01 00:00:00

  • Isolation of the lipid intermediate in peptidoglycan biosynthesis from Escherichia coli.

    abstract::The lipid intermediate in peptidoglycan biosynthesis was isolated from Escherichia coli strain W and characterized as C(55)-isoprenyl-pyrophosphoryl N-acetylmuramyl(-pentapeptide)-N-acetyl-glucosamine. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.112.3.1306-1309.1972

    authors: Umbreit JN,Strominger JL

    更新日期:1972-12-01 00:00:00

  • Mutational analysis of active site residues essential for sensing of organic hydroperoxides by Bacillus subtilis OhrR.

    abstract::Bacillus subtilis OhrR is the prototype for the one-Cys family of organic peroxide-sensing regulatory proteins. Mutational analyses indicate that the high sensitivity of the active site cysteine (C15) to peroxidation requires three Tyr residues. Y29 and Y40 from the opposing subunit of the functional dimer hydrogen bo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00879-07

    authors: Soonsanga S,Fuangthong M,Helmann JD

    更新日期:2007-10-01 00:00:00

  • Identification and functional characterization of arylamine N-acetyltransferases in eubacteria: evidence for highly selective acetylation of 5-aminosalicylic acid.

    abstract::Arylamine N-acetyltransferase activity has been described in various bacterial species. Bacterial N-acetyltransferases, including those from bacteria of the gut flora, may be involved in the metabolism of xenobiotics, thereby exerting physiopathological effects. We characterized these enzymes further by steady-state k...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.11.3417-3427.2001

    authors: Deloménie C,Fouix S,Longuemaux S,Brahimi N,Bizet C,Picard B,Denamur E,Dupret JM

    更新日期:2001-06-01 00:00:00

  • Physiological function of superoxide dismutase in glucose-limited chemostat cultures of Escherichia coli.

    abstract::Conditions for continuous culture of Escherichia coli K-12 His- Thi- under glucose limitation were established. Both the capacity for respiration, at D greater than 0.2/h, and specific activity of superoxide dismutase increased as a function of specific growth rate, whereas peroxidase and catalase were either invarian...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.130.2.805-811.1977

    authors: Hassan HM,Fridovich I

    更新日期:1977-05-01 00:00:00

  • Evidence for adenylate nucleotide transport (ATP-ADP translocation) in vesicles of Frankia sp. strain EAN1pec.

    abstract::Atractyloside and carboxyatractyloside partially inhibited nitrogenase activity (acetylene reduction) by isolated vesicles of Frankia strain EAN1pec. Extracts of disrupted vesicles showed nitrogenase activity that was not affected by the inhibitors. The vesicles accumulated ATP by an atractyloside-sensitive mechanism....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.7.3053-3057.1988

    authors: Tisa LS,Ensign JC

    更新日期:1988-07-01 00:00:00

  • Repressor gene finO in plasmids R100 and F: constitutive transfer of plasmid F is caused by insertion of IS3 into F finO.

    abstract::Fertility factor F confers bacterial conjugation, a process which involves at least 20 tra genes. Resistance plasmids such as R100, R6-5, and R1 have homology with F in the tra region. Conjugal transfer of these plasmids is, however, repressed, while transfer of F is constitutive. Repression of R transfer is due to th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.2.619-623.1987

    authors: Yoshioka Y,Ohtsubo H,Ohtsubo E

    更新日期:1987-02-01 00:00:00

  • Structural basis for biofilm formation via the Vibrio cholerae matrix protein RbmA.

    abstract::During the transition from a free-swimming, single-cell lifestyle to a sessile, multicellular state called a biofilm, bacteria produce and secrete an extracellular matrix comprised of nucleic acids, exopolysaccharides, and adhesion proteins. The Vibrio cholerae biofilm matrix contains three major protein components, R...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00374-13

    authors: Giglio KM,Fong JC,Yildiz FH,Sondermann H

    更新日期:2013-07-01 00:00:00

  • Substratum-induced morphological changes in a marine bacterium and their relevance to biofilm structure.

    abstract::The effects of surfaces on the physiology of bacteria adhering to surfaces or immobilized within biofilms are receiving more interest. A study of the effects of hydrophobic and hydrophilic substrata on the colonization behavior of a marine bacterium, SW5, revealed major differences in the morphology of SW5 on these su...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.22.6900-6906.1994

    authors: Dalton HM,Poulsen LK,Halasz P,Angles ML,Goodman AE,Marshall KC

    更新日期:1994-11-01 00:00:00

  • Conditional synthesis and utilization of 1,5-anhydroglucitol in Escherichia coli.

    abstract::A cyclic polyol, 1,5-anhydro-D-glucitol (AG), is widely detected in most organisms, although little is known about its metabolism and physiological roles. The present study demonstrates the synthesis of AG in Escherichia coli C600. The major portion of the synthesized AG was indicated to be derived from glucose retain...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.22.7138-7141.1993

    authors: Shiga Y,Mizuno H,Akanuma H

    更新日期:1993-11-01 00:00:00

  • Two segments in bacterial antizyme P22 are essential for binding and enhance degradation of lysine/ornithine decarboxylase in Selenomonas ruminantium.

    abstract::In Selenomonas ruminantium, a strictly anaerobic and gram-negative bacterium, the degradation of lysine/ornithine decarboxylase (LDC/ODC) by ATP-requiring protease(s) is accelerated by the binding of P22, which is a ribosomal protein of this strain. Amino acid sequence alignment of S. ruminantium P22 with the L10 ribo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01429-07

    authors: Yamaguchi Y,Takatsuka Y,Kamio Y

    更新日期:2008-01-01 00:00:00

  • Sporulation in D-glucosamine auxotrophs of Saccharomyces cerevisiae: meiosis with defective ascospore wall formation.

    abstract::Mutants that require exogenous D-glucosamine for growth were isolated from Saccharomyces cerevisiae X2180-1A after ethyl methane sulfonate mutagenesis. Class A auxotrophs fail to grow on yeast extract-peptone-dextrose and minimal media, whereas class B auxotrophs grow on minimal medium and readily revert to grow on ye...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.124.3.1545-1557.1975

    authors: Whelan WL,Ballou CE

    更新日期:1975-12-01 00:00:00