Two different dihydroorotate dehydrogenases in Lactococcus lactis.

Abstract:

:The pyrimidine de novo biosynthesis pathway has been characterized for a number of organisms. The general pathway consists of six enzymatic steps. In the characterization of the pyrimidine pathway of Lactococcus lactis, two different pyrD genes encoding dihydroorotate dehydrogenase were isolated. The nucleotide sequences of the two genes, pyrDa and pyrDb, have been determined. One of the deduced amino acid sequences has a high degree of homology to the Saccharomyces cerevisiae dihydroorotate dehydrogenase, and the other resembles the dihydroorotate dehydrogenase from Bacillus subtilis. It is possible to distinguish between the two enzymes in crude extracts by using different electron acceptors. We constructed mutants containing a mutated form of either one or the other or both of the pyrD genes. Only the double mutant is pyrimidine auxotrophic.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Andersen PS,Jansen PJ,Hammer K

doi

10.1128/jb.176.13.3975-3982.1994

subject

Has Abstract

pub_date

1994-07-01 00:00:00

pages

3975-82

issue

13

eissn

0021-9193

issn

1098-5530

journal_volume

176

pub_type

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