Abstract:
:Aerobic microorganisms have evolved different strategies to withstand environmental oxidative stresses generated by various reactive oxygen species (ROS). For the facultative anaerobic human oral pathogen Streptococcus mutans, the mechanisms used to protect against ROS are not fully understood, since it does not possess catalase, an enzyme that degrades hydrogen peroxide. In order to elucidate the genes that are essential for superoxide stress response, methyl viologen (MV)-sensitive mutants of S. mutans were generated via ISS1 mutagenesis. Screening of approximately 2,500 mutants revealed six MV-sensitive mutants, each containing an insertion in one of five genes, including a highly conserved hypothetical gene, SMU.1297. Sequence analysis suggests that SMU.1297 encodes a hypothetical protein with a high degree of homology to the Bacillus subtilis YtqI protein, which possesses an oligoribonuclease activity that cleaves nano-RNAs and a phosphatase activity that degrades 3'-phosphoadenosine-5'-phosphate (pAp) and 3'-phosphoadenosine-5'-phosphosulfate (pApS) to produce AMP; the latter activity is similar to the activity of the Escherichia coli CysQ protein, which is required for sulfur assimilation. SMU.1297 was deleted using a markerless Cre-loxP-based strategy; the SMU.1297 deletion mutant was just as sensitive to MV as the ISS1 insertion mutant. Complementation of the deletion mutant with wild-type SMU.1297, in trans, restored the parental phenotype. Biochemical analyses with purified SMU.1297 protein demonstrated that it has pAp phosphatase activity similar to that of YtqI but apparently lacks an oligoribonuclease activity. The ability of SMU.1297 to dephosphorylate pApS in vivo was confirmed by complementation of an E. coli cysQ mutant with SMU.1297 in trans. Thus, our results suggest that SMU.1297 is involved in superoxide stress tolerance in S. mutans. Furthermore, the distribution of homologs of SMU.1297 in streptococci indicates that this protein is essential for superoxide stress tolerance in these organisms.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Zhang J,Biswas Idoi
10.1128/JB.00184-09subject
Has Abstractpub_date
2009-07-01 00:00:00pages
4330-40issue
13eissn
0021-9193issn
1098-5530pii
JB.00184-09journal_volume
191pub_type
杂志文章abstract::In order to characterize the genome-wide transcriptional response of the hyperthermophilic, aerobic crenarchaeote Sulfolobus solfataricus to UV damage, we used high-density DNA microarrays which covered 3,368 genetic features encoded on the host genome, as well as the genes of several extrachromosomal genetic elements...
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journal_title:Journal of bacteriology
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doi:10.1128/jb.178.12.3447-3456.1996
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更新日期:2002-01-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/jb.171.1.299-302.1989
更新日期:1989-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1979-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.3.753-767.1996
更新日期:1996-02-01 00:00:00
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更新日期:2012-12-01 00:00:00
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更新日期:2009-11-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1979-01-01 00:00:00
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更新日期:1999-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:1995-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:1973-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2011-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1979-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2009-04-01 00:00:00