Biosynthesis of branched-chain amino acids in yeast: regulation of leucine biosynthesis in prototrophic and leucine auxotrophic strains.

abstract：:The Czc system of Alcaligenes eutrophus mediates resistance to cobalt, zinc, and cadmium through ion efflux catalyzed by the CzcCB2A cation-proton antiporter. DNA sequencing of the region upstream of the czcNICBADRS determinant located on megaplasmid pMOL30 revealed the 5' end of czcN and a gene for a MgtC-like protei...

journal_title：Journal of bacteriology

authors： Grosse C,Grass G,Anton A,Franke S,Santos AN,Lawley B,Brown NL,Nies DH

更新日期：1999-04-01 00:00:00

abstract：:31P and 13C nuclear magnetic resonance (NMR) experiments were performed on suspensions of the phototrophic bacterium Chromatium vinosum incubated anaerobically in the dark. 31P NMR spectra revealed that during prolonged dark incubation high ATP levels are maintained. This phenomenon was independent of the presence of ...

journal_title：Journal of bacteriology

authors： Nicolay K,van Gemerden H,Hellingwerf KJ,Konings WN,Kaptein R

更新日期：1983-08-01 00:00:00

abstract：:A lipoteichoic acid (LTA) was extracted from Listeria monocytogenes (serotype 1) by phenol-water partition and isolated by gel-filtration chromatography. The LTA exhibited amphiphilic properties by changes in gel-filtration mobility in the presence of detergent buffers and after mild base hydrolysis. In a hemagglutina...

journal_title：Journal of bacteriology

authors： Hether NW,Jackson LL

更新日期：1983-11-01 00:00:00

abstract：:The structural gene for the F sex factor outer membrane surface exclusion protein ( traT ) was cloned onto a high-copy-number plasmid where it is expressed from the phage lambda promoter pL. Conditional control over expression was provided by a temperature-sensitive lambda cI repressor. Induction of pL produced large ...

journal_title：Journal of bacteriology

authors： Minkley EG Jr

更新日期：1984-05-01 00:00:00

abstract：:It was shown previously that the disruption of the ahrC gene encoding a predicted ArgR family transcription factor results in a severe defect in biofilm formation in vitro, as well as a significant attenuation of virulence of Enterococcus faecalis strain OG1RF in multiple experimental infection models. Using transcrip...

journal_title：Journal of bacteriology

authors： Manias DA,Dunny GM

更新日期：2018-08-24 00:00:00

abstract：:FabG performs the NADPH-dependent reduction of β-keto acyl-acyl carrier protein substrates in the elongation cycle of fatty acid synthesis. We report the characterization of a temperature-sensitive mutation (fabGΔ8) in Escherichia colifabG that results from an in-frame 8-amino-acid residue deletion in the α6/α7 subdom...

journal_title：Journal of bacteriology

authors： Srinivas S,Cronan JE

更新日期：2017-04-25 00:00:00

abstract：:Previous investigations have demonstrated the existence of five Histoplasma capsulatum serotypes. Available specific fluorescent-antibody reagents stain only four of the five serotypes. Antibodies produced against the most complete H. capsulatum serotype were labeled with fluorescein isothiocyanate to develop a reagen...

journal_title：Journal of bacteriology

authors： Kaufman L,Blumer S

更新日期：1968-04-01 00:00:00

abstract：:We have identified three transposable gene-activating elements from Pseudomonas cepacia on the basis of their abilities to increase expression of the lac genes of the broad-host-range plasmid pGC91.14 (pRP1::Tn951). When introduced into auxotrophic derivatives of P. cepacia 249 (ATCC 17616), this plasmid failed to con...

journal_title：Journal of bacteriology

authors： Wood MS,Lory C,Lessie TG

更新日期：1990-04-01 00:00:00

abstract：:Molecular phylogenetic surveys have recently revealed an ecologically widespread crenarchaeal group that inhabits cold and temperate terrestrial and marine environments. To date these organisms have resisted isolation in pure culture, and so their phenotypic and genotypic characteristics remain largely unknown. To cha...

journal_title：Journal of bacteriology

authors： Schleper C,DeLong EF,Preston CM,Feldman RA,Wu KY,Swanson RV

更新日期：1998-10-01 00:00:00

abstract：:DNA uptake by natural competence is a central process underlying the genetic plasticity, biology, and virulence of the human respiratory opportunistic pathogen Streptococcus pneumoniae A study reported in this issue (J. Slager, R. Aprianto, and J.-W. Veening, J. Bacteriol. 201:e00780-18, https://doi.org/10.1128/JB.007...

journal_title：Journal of bacteriology

authors： Winkler ME,Morrison DA

更新日期：2019-06-10 00:00:00

abstract：:Four cold-sensitive mutants of Escherichia coli have been isolated which show a reduced ability to synthesize deoxyribonucleic acid at low temperature. The mutants also have a reduced ability to incorporate nucleoside triphosphates into deoxyribonucleic acid at low temperature in cell preparations made permeable with ...

journal_title：Journal of bacteriology

authors： Wehr CT,Waskell L,Glaser DA

更新日期：1975-01-01 00:00:00

abstract：:A chromosomal insertion of transposon Tn917 partially restores the expression of protease and alpha-toxin activities to PM466, a genetically defined agr-null derivative of the wild-type Staphylococcus aureus strain RN6390. In co-transduction experiments, transposon-encoded erythromycin resistance and a protease- and a...

journal_title：Journal of bacteriology

authors： McNamara PJ,Milligan-Monroe KC,Khalili S,Proctor RA

更新日期：2000-06-01 00:00:00

abstract：:Using representational difference analysis, we isolated novel meningococcal restriction-modification (R-M) systems. NmeBI, which is a homologue of the R-M system HgaI of Pasteurella volantium, was present in meningococci of the ET-5 complex and of lineage III. NmeAI was found in serogroup A, ET-37 complex, and cluster...

journal_title：Journal of bacteriology

authors： Claus H,Friedrich A,Frosch M,Vogel U

更新日期：2000-03-01 00:00:00

abstract：:Enterohemorrhagic Escherichia coli O157:H7 (EHEC) causes bloody diarrhea and hemolytic-uremic syndrome. EHEC encodes the sRNA chaperone Hfq, which is important in posttranscriptional regulation. In EHEC strain EDL933, Hfq acts as a negative regulator of the locus of enterocyte effacement (LEE), which encodes most of t...

journal_title：Journal of bacteriology

authors： Kendall MM,Gruber CC,Rasko DA,Hughes DT,Sperandio V

更新日期：2011-12-01 00:00:00

abstract：:Internal and surface structures of asci and ascospores were studied by transmission electron microscopy (TEM) and by scanning electron microscopy (SEM) to establish the character and number of ascospores within the ascus of Metschnikowia krissii. Enzyme digestion with snail gut enzymes and SEM examination suggested th...

journal_title：Journal of bacteriology

authors： Talens LT,Miller MW,Miranda M

更新日期：1973-07-01 00:00:00

abstract：:Pseudomonas syringae injects numerous bacterial proteins into host plant cells through a type 3 secretion system (T3SS). One of the first such bacterial effectors discovered, HopA1, is a protein that has unknown functions in the host cell but possesses close homologs that trigger the plant hypersensitive response in r...

journal_title：Journal of bacteriology

authors： Janjusevic R,Quezada CM,Small J,Stebbins CE

更新日期：2013-02-01 00:00:00

abstract：:Endonuclease V is specific for single-stranded DNA or for duplex DNA that contains uracil or that is damaged by a variety of agents (B. Demple and S. Linn, J. Biol. Chem. 257:2848-2855, 1982). Thus, it may be a versatile DNA repair enzyme. The protein was purified to apparent homogeneity, and from its N-terminal seque...

journal_title：Journal of bacteriology

authors： Guo G,Ding Y,Weiss B

更新日期：1997-01-01 00:00:00

abstract：:Evidence is presented that progametangia in both the plus and the minus mating types of Mucor mucedo can be induced by one substance, namely (-)-trisporic acid B. A method is described for the determination of the concentration of the sex factors (trisporone, trisporic acid B, trisporic acid C) in mated cultures of Mu...

journal_title：Journal of bacteriology

authors： Van den Ende H,Wiechmann AH,Reyngoud DJ,Hendriks T

更新日期：1970-02-01 00:00:00

abstract：:HypA and HypB are maturation proteins required for incorporation of nickel into the hydrogenase large subunit. To examine the functions of these proteins in nickel insertion, the hybF gene, which is a homolog of hypA essential for maturation of hydrogenases 1 and 2 from Escherichia coli, was overexpressed, and the pro...

journal_title：Journal of bacteriology

authors： Blokesch M,Rohrmoser M,Rode S,Böck A

更新日期：2004-05-01 00:00:00

abstract：:Thermococcus kodakarensis (formerly Thermococcus kodakaraensis) strains have been constructed with synthetic and natural DNA sequences, predicted to function as archaeal transcription terminators, identically positioned between a constitutive promoter and a beta-glycosidase-encoding reporter gene (TK1761). Expression ...

journal_title：Journal of bacteriology

authors： Santangelo TJ,Cubonová L,Skinner KM,Reeve JN

更新日期：2009-11-01 00:00:00

abstract：:A conditional developmental mutant of Mucor racemosus which is capable of oxidative energy metabolism is described. Unlike the wild-type strain the mutant was highly fermentative and exhibited the yeast morphology when grown aerobically in glucose-containing media. The high fermentative activity and yeast morphology u...

journal_title：Journal of bacteriology

authors： Borgia PT,Gokul NK,Phillips GJ

更新日期：1985-12-01 00:00:00

abstract：:Identification of new genes involved in biofilm formation is needed to understand the molecular basis of strain variation and the pathogenic mechanisms implicated in chronic staphylococcal infections. A biofilm-producing Staphylococcus aureus isolate was used to generate biofilm-negative transposon (Tn917) insertion m...

journal_title：Journal of bacteriology

authors： Cucarella C,Solano C,Valle J,Amorena B,Lasa I,Penadés JR

更新日期：2001-05-01 00:00:00

abstract：:The quorum-sensing system of Staphylococcus aureus, the accessory gene regulator (Agr) system, is responsible for increased transcription of certain exoprotein genes and decreased transcription of certain cell wall-associated proteins during the postexponential phase of growth. This regulation is important for virulen...

journal_title：Journal of bacteriology

authors： Tseng CW,Zhang S,Stewart GC

更新日期：2004-03-01 00:00:00

abstract：:A heat-stable, particulate, lipopolysaccharide-protein antigenic complex has been isolated from a virulent, encapsulated strain of Pasteurella multocida by extraction with cold, formalinized saline, and centrifugation at 105,000 x g. The original bacterial culture had been obtained from a bison that died of hemorrhagi...

journal_title：Journal of bacteriology

authors： Rebers PA,Heddleston KL,Rhoades KR

更新日期：1967-01-01 00:00:00

abstract：:A monoclonal antibody (MAb), MAb 900, which detects a 43-kDa protein present on Escherichia coli was found. Subsequently, more than 90 organisms, belonging to either the bacterial, archaeal, or eucaryal domain, were tested for reactivity to this MAb. Of the bacterial and archaeal domains, almost all species proved to ...

journal_title：Journal of bacteriology

authors： Weber S,Lottspeich F,Köhl J

更新日期：1995-01-01 00:00:00

abstract：:A mutant strain of Pediococcus cerevisiae (P. cerevisiae/PteGlu) was isolated which grows on low-folate (PteGlu) concentrations (200 pg/ml). The growth response of the parent and mutant strains to folinate (5-CHO-H(4)PteGlu) was the same. The transport of (14)C-PteGlu by P. cerevisiae/PteGlu was temperature-dependent ...

journal_title：Journal of bacteriology

authors： Mandelbaum-Shavit F,Grossowicz N

更新日期：1973-05-01 00:00:00

abstract：:LcrV, an essential piece of the Yop virulon, is encoded by the large lcrGVsycDyopBD operon. In spite of repeated efforts, the role of LcrV in the Yop virulon remains elusive. In an attempt to clarify this, we engineered a complete deletion of lcrV in the pYV plasmid of Yersinia enterocolitica E40 and characterized the...

journal_title：Journal of bacteriology

authors： Sarker MR,Neyt C,Stainier I,Cornelis GR

更新日期：1998-03-01 00:00:00

abstract：:We recently reported a novel genetic locus located in the sbcB-his region of the chromosomal map of Escherichia coli K-12 which directs the expression of group 6-positive phenotype in Shigella flexneri lipopolysaccharide, presumably due to the transfer of O-acetyl groups onto rhamnose residues of the S. flexneri O-spe...

journal_title：Journal of bacteriology

authors： Yao Z,Valvano MA

更新日期：1994-07-01 00:00:00

abstract：:Mutational analysis of the carboxy-terminal region of Escherichia coli HlyC was performed by site-directed mutagenesis. Replacement of residue Val-127 or Lys-129 reduced the activity of HlyC to about 30 or 60%, respectively, of that of the wild type, while replacement of Gly-128 reduced the activity to less than 1% of...

journal_title：Journal of bacteriology

authors： Guzmán-Verri C,García F,Arvidson S

更新日期：1997-09-01 00:00:00

abstract：:A population of Bacillus subtilis can be fractionated into at least two components on the basis of their buoyant densities on a Renografin gradient. In competent cultures, most, and perhaps all, of the competent bacteria appear in the lighter fraction which composes 2 to 10% of the bulk population. These "lighter" bac...

journal_title：Journal of bacteriology

authors： Cahn FH,Fox MS

更新日期：1968-03-01 00:00:00