Abstract:
:When soluble extracts of the extreme acidothermophilic archaeon Sulfolobus solfataricus were incubated with [gamma-(32)P]ATP, several proteins were radiolabeled. One of the more prominent of these, which migrated with a mass of approximately 46 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), was purified by column chromatography and SDS-PAGE and subjected to amino acid sequence analysis via both the Edman technique and mass spectroscopy. The best match to the partial sequence obtained was the potential polypeptide product of open reading frame sso0417, whose DNA-derived amino acid sequence displayed many features reminiscent of the 2,3-diphosphoglycerate-independent phosphoglycerate (PGA) mutases [iPGMs]. Open reading frame sso0417 was therefore cloned, and its protein product was expressed in Escherichia coli. Assays of its catalytic capabilities revealed that the protein was a moderately effective PGA mutase that also exhibited low levels of phosphohydrolase activity. PGA mutase activity was dependent upon the presence of divalent metal ions such as Co(2+) or Mn(2+). The recombinant protein underwent autophosphorylation when incubated with either [gamma-(32)P]ATP or [gamma-(32)P]GTP. The site of phosphorylation was identified as Ser(59), which corresponds to the catalytically essential serine residue in bacterial and eucaryal iPGMs. The phosphoenzyme intermediate behaved in a chemically and kinetically competent manner. Incubation of the (32)P-labeled phosphoenzyme with 3-PGA resulted in the disappearance of radioactive phosphate and the concomitant appearance of (32)P-labeled PGA at rates comparable to those measured in steady-state assays of PGA mutase activity.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Potters MB,Solow BT,Bischoff KM,Graham DE,Lower BH,Helm R,Kennelly PJdoi
10.1128/jb.185.7.2112-2121.2003keywords:
subject
Has Abstractpub_date
2003-04-01 00:00:00pages
2112-21issue
7eissn
0021-9193issn
1098-5530journal_volume
185pub_type
杂志文章abstract::The kps locus for polysialic acid capsule expression in Escherichia coli K1 is composed of a central group of biosynthetic neu genes, designated region 2, flanked on either side by region 1 or region 3 kps genes with poorly defined functions. Chromosomal mutagenesis with MudJ and subsequent complementation analysis, m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.24.8018-8023.1993
更新日期:1993-12-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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更新日期:1971-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.118.3.1186-1189.1974
更新日期:1974-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.9.4376-4378.1987
更新日期:1987-09-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.84.4.730-734.1962
更新日期:1962-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.16.4875-4882.1994
更新日期:1994-08-01 00:00:00
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doi:10.1128/JB.186.23.8074-8082.2004
更新日期:2004-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.22.7034-7042.1999
更新日期:1999-11-01 00:00:00
abstract::The effect of adding hemin to anaerobically grown cells of a strain of Staphylococcus epidermidis, which was heme-deficient due to anaerobic growth, has been examined. Cells grown anaerobically in media containing hemin exhibited a marked increase in several oxidative activities as compared with cells grown anaerobica...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.1.278-285.1967
更新日期:1967-01-01 00:00:00
abstract::Tabak, Henry H. (Robert A. Taft Sanitary Engineering Center, Cincinnati, Ohio), Cecil W. Chambers, and Paul W. Kabler. Microbial metabolism of aromatic carbon compounds. I. Decomposition of phenolic compounds and aromatic hydrocarbons by phenol-adapted bacteria. J. Bacteriol. 87:910-919. 1964.-Bacteria from soil and r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.4.910-919.1964
更新日期:1964-04-01 00:00:00
abstract::In the course of study on ribonucleic acid (RNA) metabolism during sporulation in Saccharomyces cerevisiae, a new species of RNA (20S) was observed in sporulating cells by polyacrylamide gel electrophoresis. The relative content of this RNA to total RNA increased linearly early in sporulation. Labeled adenine was pref...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.3.826-830.1971
更新日期:1971-03-01 00:00:00
abstract::Genetic transformation of Sulfolobus acidocaldarius by a multiply marked pyrE gene provided a high-resolution assay of homologous recombination in a hyperthermophilic archaeon. Analysis of 100 Pyr(+) transformants revealed that this recombination system could transfer each of 23 nonselected base pair substitutions to ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2010-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.117.3.1178-1183.1974
更新日期:1974-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.167.2.455-461.1986
更新日期:1986-08-01 00:00:00
abstract::The application of a simple negative staining technique and freeze-etching has revealed detailed information on the species-specific surface structure of spores and aerial hyphae of streptomycetes. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.101.1.318-322.1970
更新日期:1970-01-01 00:00:00
abstract::Dobrogosz, Walter J. (University of Illinois, Urbana) and Ralph D. DeMoss. Pentose utilization by Pediococcus pentosaceus. J. Bacteriol. 85:1356-1364. 1963.-Data are presented which indicate that pentoses are metabolized by Pediococcus pentosaceus through the use of an inducible phosphoketolase pathway. The utilizatio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.6.1356-1364.1963
更新日期:1963-06-01 00:00:00
abstract::Formate is an important energy substrate for sulfate-reducing bacteria in natural environments, and both molybdenum- and tungsten-containing formate dehydrogenases have been reported in these organisms. In this work, we studied the effect of both metals on the levels of the three formate dehydrogenases encoded in the ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00042-11
更新日期:2011-06-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1977-11-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1997-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.21.5803-5807.1998
更新日期:1998-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.1.234-238.1975
更新日期:1975-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00176-06
更新日期:2006-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2011-08-01 00:00:00
abstract::On the basis of information available in the literature, gluconate dissimilation in Escherichia coli is thought to occur via the hexose monophosphate pathway. Evidence is presented in this study that gluconate is catabolized in this organism via an inducible Entner-Doudoroff pathway. This evidence is based on chromato...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.3.941-949.1967
更新日期:1967-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00192-11
更新日期:2011-08-01 00:00:00
abstract::Transcription of the gyrA and gyrB genes, which encode the subunits of DNA gyrase, increases in response to DNA relaxation. Previous studies have shown that a small segment of DNA extending from the -10 consensus hexamer to the start of transcription encodes the sequence determinants for this response. In this study, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.19.5999-6006.1994
更新日期:1994-10-01 00:00:00
abstract::Escherichia coli has been used as a platform host for studying the production of free fatty acids (FFA) and other energy-dense compounds useful in biofuel applications. Most of the FFA produced by E. coli are found extracellularly. This finding suggests that a mechanism for transport across the cell envelope exists, y...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01477-12
更新日期:2013-01-01 00:00:00