Characterization of chloramphenicol acetyltransferase from chloramphenicol-resistant Staphylococcus aureus.

abstract：:Previously, we identified a gene (aldA) from Myxococcus xanthus, which we suggested encoded the enzyme alanine dehydrogenase on the basis of similarity to known Ald protein sequences (M. J. Ward, H. Lew, A. Treuner-Lange, and D. R. Zusman, J. Bacteriol. 180:5668-5675, 1998). In this study, we have confirmed that aldA ...

journal_title：Journal of bacteriology

authors： Ward MJ,Lew H,Zusman DR

更新日期：2000-01-01 00:00:00

abstract：:We have developed a method, using laser, optical tweezers and direct microscopic analysis of reproductive potential and membrane integrity, to assess single-cell viability in a stationary-phase Escherichia coli population. It is demonstrated here that a reduction in cell integrity, determined by using the fluorescent ...

journal_title：Journal of bacteriology

authors： Ericsson M,Hanstorp D,Hagberg P,Enger J,Nyström T

更新日期：2000-10-01 00:00:00

abstract：:Cells of Clostridium thermosaccharolyticum grown under strict anaerobiosis (modified Hungate technique) were examined during growth and sporulation by employing Nomarski interference-contrast and Zernike phase-contrast optics to delineate the sequence of morphological changes leading to the formation of free, mature s...

journal_title：Journal of bacteriology

authors： Eller C,Ordal ZJ

更新日期：1972-09-01 00:00:00

abstract：:An enzyme which liberates Pi from myo-inositol hexaphosphate (phytic acid) was shown to be present in culture filtrates of Bacillus subtilis. It was purified until it was homogeneous by ultracentrifugation, but it still showed two isozymes on polyacrylamide gel electrophoresis. The enzyme differed from other previousl...

journal_title：Journal of bacteriology

authors： Powar VK,Jagannathan V

更新日期：1982-09-01 00:00:00

abstract：:Eleven conditional lethal dnaG(Ts) mutations were located by chemical cleavage of heteroduplexes formed between polymerase chain reaction-amplified DNAs from wild-type and mutant dnaG genes. This entailed end labeling one DNA strand of the heteroduplex, chemically modifying the strands with hydroxylamine or osmium tet...

journal_title：Journal of bacteriology

authors： Grompe M,Versalovic J,Koeuth T,Lupski JR

更新日期：1991-02-01 00:00:00

abstract：:Among mutants which require isoleucine, but not valine, for growth, we have found two distinguishable classes. One is defective in the biosynthetic enzyme threonine deaminase (l-threonine hydro-lyase, deaminating, EC 4.2.1.16) and the other has an altered isoleucyl transfer ribonucleic acid (tRNA) synthetase [l-isoleu...

journal_title：Journal of bacteriology

authors： Iaccarino M,Berg P

更新日期：1971-02-01 00:00:00

abstract：:The mechanism for the extrusion of Na+ from Mycoplasma gallisepticum cells was examined. Na+ efflux from cells was studied by diluting 22Na+-loaded cells into an isoosmotic NaCl solution and measuring the residual 22Na+ in the cells. Uphill 22Na+ efflux was found to be glucose dependent and linear with time over a 60-...

journal_title：Journal of bacteriology

authors： Shirvan MH,Schuldiner S,Rottem S

更新日期：1989-08-01 00:00:00

abstract：:In Escherichia coli and Salmonella, the c-di-GMP effector YcgR inhibits flagellar motility by interacting directly with the motor to alter both its bias and speed. Here, we demonstrate that in both of these bacteria, YcgR acts sequentially, altering motor bias first and then decreasing motor speed. We show that when c...

journal_title：Journal of bacteriology

authors： Nieto V,Partridge JD,Severin GB,Lai RZ,Waters CM,Parkinson JS,Harshey RM

更新日期：2019-12-06 00:00:00

abstract：:Open reading frame orf192, which is located immediately upstream of the aerobic repressor gene crtJ, was genetically and biochemically demonstrated to code for a second aerobic repressor (AerR) of photosynthesis gene expression in Rhodobacter capsulatus. Promoter-mapping studies indicate that crtJ has its own promoter...

journal_title：Journal of bacteriology

authors： Dong C,Elsen S,Swem LR,Bauer CE

更新日期：2002-05-01 00:00:00

abstract：:The blp locus of a type 6A strain of Streptococcus pneumoniae encodes a two-peptide bacteriocin, pneumocin MN, which mediates intraspecies competition during mouse nasopharyngeal colonization. This locus is regulated by a quorum-sensing mechanism consisting of a dedicated two-component regulatory system and a peptide ...

journal_title：Journal of bacteriology

authors： Dawid S,Sebert ME,Weiser JN

更新日期：2009-03-01 00:00:00

abstract：:The pathogenic bacterium Bacillus anthracis has become the subject of intense study as a result of its use in a bioterrorism attack in the United States in September and October 2001. Previous studies suggested that B. anthracis Ames Ancestor, the original Ames fully virulent plasmid-containing isolate, was the ideal ...

journal_title：Journal of bacteriology

authors： Ravel J,Jiang L,Stanley ST,Wilson MR,Decker RS,Read TD,Worsham P,Keim PS,Salzberg SL,Fraser-Liggett CM,Rasko DA

更新日期：2009-01-01 00:00:00

abstract：:Eight species representative of the serological diversity of the Mycoplasmatales were tested for their ability to incorporate radiolabeled nucleic acid precursors into acid-insoluble material. Cultures in complex growth medium were centrifuged and resuspended in minimal essential medium (Eagle). For Acholeplasma laidl...

journal_title：Journal of bacteriology

authors： McIvor RS,Kenny GE

更新日期：1978-08-01 00:00:00

abstract：:Manganese is accumulated in Bacillus subtilis by a highly specific active transport system. This trace element "pump" is insensitive to added magnesium or calcium and preferentially accumulates manganese in the presence of cobalt, iron, and copper. Manganese uptake in B. subtilis is inhibited by cyanide, azide, pentac...

journal_title：Journal of bacteriology

authors： Eisenstadt E,Fisher S,Der CL,Silver S

更新日期：1973-03-01 00:00:00

abstract：:We investigated an osmotic remedial mutant of Saccharomyces which was deficient in galactose-1-phosphate uridyl transferase. Both the rate of growth and the transferase activity of the mutant culture were dependent on the osmotic activity of the growth medium. Organic and ionic solutes proved to be equally effective i...

journal_title：Journal of bacteriology

authors： Bassel J,Douglas HC

更新日期：1968-03-01 00:00:00

abstract：:Several Pseudomonas solanacearum strains which produced no detectable extracellular polysaccharide (EPS) in planta had been reported to remain highly virulent when tested at high inoculum concentrations (P. Xu, M. Iwata, S. Leong, and L. Sequeira, J. Bacteriol. 172:3946-3951, 1990; P. Xu, S. Leong, and L. Sequeira, J....

journal_title：Journal of bacteriology

authors： Kao CC,Barlow E,Sequeira L

更新日期：1992-02-01 00:00:00

abstract：:Insertion-duplication mutagenesis was used to generate mutants of Streptococcus pneumoniae that produced truncated forms of PspA (pneumococcal surface protein A). The truncated products, representing from 20 to 80% of the complete PspA molecule, were all secreted from the cell and could be detected in unconcentrated c...

journal_title：Journal of bacteriology

authors： Yother J,Handsome GL,Briles DE

更新日期：1992-01-01 00:00:00

abstract：:The composition of the Pseudomonas solanacearum lipolysaccharide (LPS) was found to be similar to that described for the LPS of enterobacteria. The lipid A contained fatty acids and glucosamine in a molar ratio of 5:2. The LPS fraction contained 2-keto-3-deoxyoctulosonic acid, L-glycero-D-mannoheptose, hexoses (glucos...

journal_title：Journal of bacteriology

authors： Drigues P,Demery-Lafforgue D,Trigalet A,Dupin P,Samain D,Asselineau J

更新日期：1985-05-01 00:00:00

abstract：:Target analyses of the induction of rho(-) mutants indicated that the cytoplasm of an aerobic nonrepressed yeast contains approximately 20 mutable units, whereas repressed cells exhibit approximately 3. Aerobic adaptation of fully repressed cells brings about an increase in these cytoplasmic units which continues afte...

journal_title：Journal of bacteriology

authors： Allen NE,MacQuillan AM

更新日期：1969-03-01 00:00:00

abstract：:Escherichia coli K-12, polAl(-) is a mutant strain whose extracts are deficient in Kornberg deoxyribonucleic acid (DNA) polymerase activity. We have compared the mutant and parental strains on the basis of a number of responses to ultraviolet (UV) and X-irradiation. For both types of radiation, the mutant is more sens...

journal_title：Journal of bacteriology

authors： Paterson MC,Boyle JM,Setlow RB

更新日期：1971-07-01 00:00:00

abstract：:Certain gram-negative pathogens are known to control virulence gene expression through cell-cell communication via small diffusible signal molecules termed autoinducers. This intercellular signal transduction mechanism termed quorum sensing depends on the interaction of an N-acylhomoserine lactone (AHL) auto-inducer m...

journal_title：Journal of bacteriology

authors： Milton DL,Hardman A,Camara M,Chhabra SR,Bycroft BW,Stewart GS,Williams P

更新日期：1997-05-01 00:00:00

abstract：:Competent cell-deoxyribonucleic acid (DNA) interactions were examined using tritium-labeled homologous or heterologous native or denatured DNAs and competent Streptococcus sanguis Wicky cells (strain WE4). The DNAs used were extracted from WE4 cells, Escherichia coli B cells, and E. coli bacteriophages T2, T4, T6, and...

journal_title：Journal of bacteriology

authors： Ranhand JM

更新日期：1980-05-01 00:00:00

abstract：:Extracellular fibrils are involved in cell cohesion and cell development in Myxococcus xanthus. One group of social motility mutants, Dsp, is unable to produce extracellular fibrils; these mutants also lose the abilities to cohere and to develop. Extracellular fibrils isolated from vegetative wild-type cells and added...

journal_title：Journal of bacteriology

authors： Chang BY,Dworkin M

更新日期：1994-12-01 00:00:00

abstract：:Bacillus anthracis, the causative agent of anthrax, replicates as chains of vegetative cells by regulating the separation of septal peptidoglycan. Surface (S)-layer proteins and associated proteins (BSLs) function as chain length determinants and bind to the secondary cell wall polysaccharide (SCWP). In this study, we...

journal_title：Journal of bacteriology

authors： Liszewski Zilla M,Chan YG,Lunderberg JM,Schneewind O,Missiakas D

更新日期：2015-01-01 00:00:00

abstract：:Many gram-negative bacteria harbor a copper/zinc-containing superoxide dismutase (CuZnSOD) in their periplasms. In pathogenic bacteria, one role of this enzyme may be to protect periplasmic biomolecules from superoxide that is released by host phagocytic cells. However, the enzyme is also present in many nonpathogens ...

journal_title：Journal of bacteriology

authors： Korshunov S,Imlay JA

更新日期：2006-09-01 00:00:00

abstract：:During intraperiplasmic growth of Bdellovibrio bacteriovorus on Escherichia coli, the substrate cell peptidoglycan is extensively modified as it is converted to bdelloplast peptidoglycan. The initially lysozyme-sensitive peptidoglycan of E. coli was rapidly converted to a lysozyme-resistant form. The conversion was du...

journal_title：Journal of bacteriology

authors： Thomashow MF,Rittenberg SC

更新日期：1978-09-01 00:00:00

abstract：:Rhizobium leguminosarum strain VF39SM contains two plasmids that have previously been shown to be self-transmissible by conjugation. One of these plasmids, pRleVF39b, is shown in this study to carry a set of plasmid transfer genes that differs significantly from conjugation systems previously studied in the rhizobia b...

journal_title：Journal of bacteriology

authors： Ding H,Yip CB,Hynes MF

更新日期：2013-01-01 00:00:00

abstract：:Two acid-inducible genes, aniC and aciK, that require anaerobiosis and tyrosine for expression were identified as orf326a encoding a potential amino acid/polyamine antiporter and hyaB encoding hydrogenase I, respectively. Cyclic AMP (cAMP) receptor protein, cAMP, and TyrR, regulator of aromatic amino acid metabolism, ...

journal_title：Journal of bacteriology

authors： Park KR,Giard JC,Eom JH,Bearson S,Foster JW

更新日期：1999-01-01 00:00:00

abstract：UNLABELLED:It is well established that the RpoN-RpoS sigma factor (σ(54)-σ(S)) cascade plays an essential role in differential gene expression during the enzootic cycle of Borrelia burgdorferi, the causative agent of Lyme disease. The RpoN-RpoS pathway is activated by the response regulator/σ(54)-dependent activator (a...

journal_title：Journal of bacteriology

authors： Yin Y,Yang Y,Xiang X,Wang Q,Yang ZN,Blevins J,Lou Y,Yang XF

更新日期：2016-04-28 00:00:00

abstract：:Sensory adaptation by the chemotaxis system of Escherichia coli requires adjustments of the extent of methyl esterification of the chemotaxis receptor proteins. One mechanism utilized by E. coli to make such adjustments is to control the activity of CheB, the enzyme responsible for removing receptor methyl ester group...

journal_title：Journal of bacteriology

authors： Stewart RC,Roth AF,Dahlquist FW

更新日期：1990-06-01 00:00:00

abstract：:In order to study the Salmonella typhimurium cobalamin biosynthetic pathway, the S. typhimurium cob operon was isolated and cloned into Escherichia coli. This approach has given the new host of the cob operon the ability to make cobalamins de novo, an ability that had probably been lost by this organism. In total, 20 ...

journal_title：Journal of bacteriology

authors： Raux E,Lanois A,Levillayer F,Warren MJ,Brody E,Rambach A,Thermes C

更新日期：1996-02-01 00:00:00