Abstract:
:Aim: This study aimed to identify novel miRNAs (miRs) as regulators of UGT1A gene expression and to evaluate them as potential risk factors for the development of liver fibrosis/cirrhosis. Materials & methods: miRNA target sites in UDP-glucuronosyltransferase 1A (UGT1A) 3'-UTR were predicted and confirmed by luciferase assays, quantitative real-time PCR and western blot using HEK293, HepG2 and Huh7 cells. UGT1A and miRNA expression were analyzed in cirrhotic patients and a mouse model of alcoholic liver fibrosis. Results: miR-214-5p and miR-486-3p overexpression reduced UGT1A mRNA, protein levels and enzyme activity in HepG2 and Huh7 cells. miR-486-3p was upregulated in cirrhotic patients and fibrotic mice livers, whereas UGT1A mRNA levels were reduced. Conclusion: In conclusion, we identified two novel miRNAs capable to repress UGT1A expression in vitro and in vivo. Furthermore, miR-486-3p may represent a potential risk factor for the development or progression of liver fibrosis/cirrhosis by means of a reduced UGT1A-mediated detoxification activity.
journal_name
Epigenomicsjournal_title
Epigenomicsauthors
Paulusch S,Kalthoff S,Landerer S,Jansen C,Schierwagen R,Klein S,Trebicka J,Strassburg CPdoi
10.2217/epi-2020-0244subject
Has Abstractpub_date
2021-01-12 00:00:00eissn
1750-1911issn
1750-192Xpub_type
杂志文章相关文献
Epigenomics文献大全abstract:AIM:We performed a study to identify the role of microRNA in thymoma. PATIENTS & METHODS:One hundred twenty-three thymoma patients with clinical information and miRNA expression data from The Cancer Genome Atlas were included in the study. Comprehensive bioinformatics analysis was integrated in our analysis. RESULTS ...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi-2016-0161
更新日期:2017-05-01 00:00:00
abstract::Proper immune function is the result of multiple cell commitment and differentiation steps, and adequate control of activation mechanisms. Deregulation of transcriptional programs in immune cells leads to the development of hematological malignancies, autoimmune diseases or immunodeficiencies. In this sense, epigeneti...
journal_title:Epigenomics
pub_type: 杂志文章,评审
doi:10.2217/epi.11.99
更新日期:2011-12-01 00:00:00
abstract:AIM:Aberrant epigenetic events are important contributors to the pathogenesis of different types of cancers and dietary botanicals with epigenetic properties can influence early cancer development leading to cancer prevention effects. We sought to investigate potential combinatorial effects of bioactive dietary compone...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi-2016-0024
更新日期:2016-08-01 00:00:00
abstract::This study aimed to unveil the functional roles of LINC00221 in hepatocellular carcinoma (HCC). A discovery cohort and a validation cohort were respectively used to identify and verify the clinical value of LINC00221 in HCC. Bioinformatics analysis was performed to explore its potential mechanisms. LINC00221 was upreg...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi-2020-0363
更新日期:2021-01-07 00:00:00
abstract::Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is widely used for mapping histone modifications, histone proteins, chromatin regulators, transcription factors and other DNA-binding proteins. It has played a significant role in our understanding of disease mechanisms and in exploring epigenetic changes...
journal_title:Epigenomics
pub_type: 杂志文章,评审
doi:10.2217/epi-2016-0053
更新日期:2016-09-01 00:00:00
abstract::Alterations in DNA methylation have been associated with cognitive decline and Alzheimer's disease. A recent study of mild cognitive impairment (MCI) reported a significant association between annual decline in cognitive function and the rs11887120 SNP located in DNMT3A, a gene implicated in DNA methylation. Here, we ...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi-2015-0014
更新日期:2016-05-01 00:00:00
abstract::Aim: To dynamically analyze the differential m6A methylation during the progression and reversal of hepatic fibrosis. Materials & methods: We induced hepatic fibrosis in C57/BL6 mice by intraperitoneal injection of CCl4. The reversal model of hepatic fibrosis was established by stopping drug after continuous injection...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi-2019-0365
更新日期:2020-10-01 00:00:00
abstract::Protein methyltransferases (PMT)s play essential roles in many biological processes through methylation of histones and diverse nonhistone substrates. Dysregulation of these enzymes has been implicated in many diseases including cancers. While PMT-associated biology can be probed via genetic perturbation, this approac...
journal_title:Epigenomics
pub_type: 杂志文章,评审
doi:10.2217/epi.15.87
更新日期:2015-01-01 00:00:00
abstract::Aim: We examined methylation changes in cell-free DNA (cfDNA) in metastatic castration-resistant prostate cancer (mCRPC) during treatment. Patients & methods: Genome-wide methylation analysis of sequentially collected cfDNA samples derived from mCRPC patients undergoing androgen-targeting therapy was performed. Result...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi-2020-0173
更新日期:2020-08-01 00:00:00
abstract::Aim: The contribution of miRNAs as epigenetic regulators of sexually dimorphic gene expression in the placenta is unknown. Materials & methods: 382 placentas from the extremely low gestational age newborns (ELGAN) cohort were evaluated for expression levels of 37,268 mRNAs and 2,102 miRNAs using genome-wide RNA-sequen...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi-2020-0062
更新日期:2020-09-01 00:00:00
abstract::For the first time, the development of next-generation sequencing technologies has brought about tools to investigate epigenetic alterations in an unbiased, yet genome-wide approach. The importance of this innovative technology is undeniable since it has already been established that changes in DNA methylation play an...
journal_title:Epigenomics
pub_type: 杂志文章,评审
doi:10.2217/epi.09.50
更新日期:2010-04-01 00:00:00
abstract:AIM:To characterize the genotypic and phenotypic extent of multilocus imprinting disturbances (MLID). MATERIALS & METHODS:We analyzed 37 patients with imprinting disorders (explorative cohort) for DNA methylation changes using the Infinium HumanMethylation450 BeadChip. For validation, three independent cohorts with im...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi-2016-0007
更新日期:2016-06-01 00:00:00
abstract:AIM:To assess the associations between gestational diabetes mellitus (GDM) and DNA methylation levels at genes related to energy metabolism. PATIENTS & METHODS:Ten loci were selected from our recent epigenome-wide association study on GDM. DNA methylation levels were quantified by bisulfite pyrosequencing in 80 placen...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi.15.72
更新日期:2015-10-01 00:00:00
abstract::Manipulation of chromatin, in which genomic DNA is packaged, is a fundamental requirement for all DNA-based metabolic processes in eukayotic cells. Histone variant incorporation, histone post-translational modifications, and ATP-dependent chromatin remodeling are three major strategies for chromatin manipulation, and ...
journal_title:Epigenomics
pub_type: 杂志文章,评审
doi:10.2217/epi.11.14
更新日期:2011-06-01 00:00:00
abstract::Children exposed to early-life adversity carry a greater risk of poor health and disease into adulthood. This increased disease risk is shadowed by changes in the epigenome. Epigenetics can change gene expression to modify disease risk; unfortunately, how epigenetics are changed by the environment is unclear. It is kn...
journal_title:Epigenomics
pub_type: 杂志文章,评审
doi:10.2217/epi.15.74
更新日期:2015-10-01 00:00:00
abstract:AIM:Imprinted genes exhibit expression in a parent-of-origin-dependent manner and are critical for child development. Recent limited evidence suggests that prenatal exposure to phthalates, ubiquitous endocrine disruptors, can affect their epigenetic dysregulation. MATERIALS & METHODS:We quantified DNA methylation of n...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi-2017-0178
更新日期:2018-07-01 00:00:00
abstract:AIM:In order to identify regulators of foam cells, we studied the H3K27 demethylase Kdm6b (also known as Jmjd3), a known regulator of macrophages, in controlling the transcriptional profile of foam cells. MATERIALS & METHODS:Foam cells from Kdm6b-deleted or Kdm6b wild-type mice were isolated and used for RNA-sequencin...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi-2016-0152
更新日期:2017-04-01 00:00:00
abstract:AIM:To assess the correlation between KDM6B and estrogen receptor β (ERβ) expression in malignant pleural mesothelioma (MPM). MATERIALS & METHODS:We evaluated gene expression by in silico analysis of microarray data, real-time PCR and western blot in MPM tumors and cell lines. RESULTS & CONCLUSION:We report a strong ...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi-2016-0025
更新日期:2016-09-01 00:00:00
abstract::This article focuses on the epigenetic alterations of aberrant promoter hypermethylation of genes, and histone modifications or RNA interference in cancer cells. Current knowledge of the hypermethylation of allele(s) in classical tumor suppressor genes in inherited and sporadic cancer, candidate tumor suppressor and o...
journal_title:Epigenomics
pub_type: 杂志文章,评审
doi:10.2217/epi.10.64
更新日期:2011-02-01 00:00:00
abstract::The GTC Cancer Summit: Novel Approaches to Drug Discovery was divided into two parallel tracks: the 2nd Cancer Epigenetics Conference, and the Protein Kinases and Drug Design Conference. The 2nd Cancer Epigenetics Conference focused on exciting changes in drug discovery that include an unprecedented private and public...
journal_title:Epigenomics
pub_type:
doi:10.2217/epi.13.9
更新日期:2013-04-01 00:00:00
abstract::Fibrosis is the excess deposition of extracellular matrix components which occur in multiple organs and ultimately leads to organ failure. Long noncoding RNAs (lncRNAs) are a kind of noncoding RNAs longer than approximately 200 nucleotides with no protein-encoding capacity. A growing body of evidence suggests that lnc...
journal_title:Epigenomics
pub_type: 杂志文章,评审
doi:10.2217/epi-2017-0020
更新日期:2017-09-01 00:00:00
abstract::Aim: To identify pregnancy-associated changes in cervical noncoding RNA (ncRNA), including miRNA and long noncoding RNA (lncRNA), and their potential effects on biologic processes. Materials & methods: We enrolled 21 pregnant women with term deliveries (≥37 weeks' gestation) in a prospective cohort and collected cervi...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi-2019-0231
更新日期:2020-06-01 00:00:00
abstract::DNA modifications represent an integral part of the epigenome and they have a pivotal role in regulation of genome function. Despite the wide variety of analytical techniques that have been developed to detect DNA modifications, their investigation at the single-genome level is only beginning to emerge. In contrast to...
journal_title:Epigenomics
pub_type: 杂志文章,评审
doi:10.2217/epi.12.30
更新日期:2012-08-01 00:00:00
abstract::Alzheimer's disease (AD) and Parkinson's disease (PD) are two common neurodegenerative diseases that result in the progressive damage or death of neurons. Environmental agents have the potential to damage the developing and mature nervous system, resulting in neurodegenerative diseases. Heritable changes in gene expre...
journal_title:Epigenomics
pub_type: 杂志文章,评审
doi:10.2217/epi.10.43
更新日期:2010-10-01 00:00:00
abstract:AIMS:Epigenetic signatures of germline cells are dynamically reprogrammed to induce appropriate differentiation, development and sex specification. We investigated sex-specific epigenetic changes in mouse fetal germ cells (FGCs) and neonatal germ cells. MATERIALS & METHODS:Six histone marks in mouse E13.5 FGCs and P1 ...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi-2018-0193
更新日期:2019-04-01 00:00:00
abstract:AIM:We compared predictive modeling approaches to estimate placental methylation using cord blood methylation. MATERIALS & METHODS:We performed locus-specific methylation prediction using both linear regression and support vector machine models with 174 matched pairs of 450k arrays. RESULTS:At most CpG sites, both ap...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi-2016-0109
更新日期:2017-03-01 00:00:00
abstract:AIM:This study aims to couple DNA methylation changes and evolution of retrogenes. MATERIALS & METHODS:A new two-step strategy was developed to screen retrogenes. Further, reduced representation bisulfite sequencing and RNA-seq data of eight tissues were used to analyze retrogenes. RESULTS:A total of 964 retrocopies ...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi-2017-0071
更新日期:2018-02-01 00:00:00
abstract::DNA methylation is widely studied in the context of cancer. However, the rediscovery of 5-hydroxymethylation of DNA adds a new layer of complexity to understanding the epigenetic basis of development and disease, including carcinogenesis. There have been significant advances in techniques for the detection of 5-hydrox...
journal_title:Epigenomics
pub_type: 杂志文章,评审
doi:10.2217/epi.13.69
更新日期:2013-12-01 00:00:00
abstract::Aim: The role of circRNAs in esophageal squamous cell cancer (ESCC) remains unclear. Materials & methods: Here we profiled six pair plasma circRNA in ESCC based on RNA sequencing, and then verified the elevation of hsa_circ_0004771 in 20 cancer tissues and 105 pair case-control plasma samples by quantitative reverse t...
journal_title:Epigenomics
pub_type: 杂志文章
doi:10.2217/epi-2019-0404
更新日期:2020-04-01 00:00:00
abstract::The examination of potential roles of epigenetic alterations in the pathogenesis of psychotic diseases have become an essential alternative in recent years as genetic studies alone are yet to uncover major gene(s) for psychosis. Here, we describe the current state of knowledge from the gene-specific and genome-wide st...
journal_title:Epigenomics
pub_type: 杂志文章,评审
doi:10.2217/epi.14.85
更新日期:2015-01-01 00:00:00