Dynamics of the cell-free DNA methylome of metastatic prostate cancer during androgen-targeting treatment.


:Aim: We examined methylation changes in cell-free DNA (cfDNA) in metastatic castration-resistant prostate cancer (mCRPC) during treatment. Patients & methods: Genome-wide methylation analysis of sequentially collected cfDNA samples derived from mCRPC patients undergoing androgen-targeting therapy was performed. Results: Alterations in methylation states of genes previously implicated in prostate cancer progression were observed and patients that maintained methylation changes throughout therapy tended to have a longer time to clinical progression. Importantly, we also report that markers associated with a highly aggressive form of the disease, neuroendocrine-CRPC, were associated with a faster time to clinical progression. Conclusion: Our findings highlight the potential of monitoring the cfDNA methylome during therapy in mCRPC, which may serve as predictive markers of response to androgen-targeting agents.






Peter MR,Bilenky M,Isserlin R,Bader GD,Shen SY,De Carvalho DD,Hansen AR,Hu P,Fleshner NE,Joshua AM,Hirst M,Bapat B




Has Abstract


2020-08-01 00:00:00












  • Epigenetic biomarkers for human sepsis and septic shock: insights from immunosuppression.

    abstract::Sepsis is a life-threatening condition that occurs when the body responds to an infection damaging its own tissues. Sepsis survivors sometimes suffer from immunosuppression increasing the risk of death. To our best knowledge, there is no 'gold standard' for defining immunosuppression except for a composite clinical en...


    pub_type: 杂志文章


    authors: Beltrán-García J,Osca-Verdegal R,Romá-Mateo C,Carbonell N,Ferreres J,Rodríguez M,Mulet S,García-López E,Pallardó FV,García-Giménez JL

    更新日期:2020-04-01 00:00:00

  • Epigenetics and brain evolution.

    abstract::Fundamental aspects of mammalian brain evolution occurred in the context of viviparity and placentation brought about by the epigenetic regulation of imprinted genes. Since the fetal placenta hormonally primes the maternal brain, two genomes in one individual are transgenerationally co-adapted to ensure maternal care ...


    pub_type: 杂志文章,评审


    authors: Keverne EB

    更新日期:2011-04-01 00:00:00

  • 4-Nonylphenol-enhanced EZH2 and RNF2 expression, H3K27me3 and H2AK119ub1 marks resulting in silencing of p21CDKN1A in vitro.

    abstract::Aim: To examine the impact of 4-nonylphenol (4-NP), on the expression of polycomb repressive complexes and cellular proliferation. Materials & methods: Cell proliferation assays, quantitative PCR, Western blotting, luciferase reporter assay, chromatin immunoprecipitation-quantitative PCR were used for the study. Resul...


    pub_type: 杂志文章


    authors: Ghosh K,Chatterjee B,Maheswari U,Athifa M,Kanade SR

    更新日期:2019-06-01 00:00:00

  • Alzheimer's disease DNA methylome of pyramidal layers in frontal cortex: laser-assisted microdissection study.

    abstract:OBJECTIVE:To study DNA methylation patterns of cortical pyramidal layers susceptible to late-onset Alzheimer's disease (LOAD) neurodegeneration. METHODS:Laser-assisted microdissection to select pyramidal layers' cells in frontal cortex of 32 human brains (18 LOAD) and Infinium DNA Methylation 450K analysis were perfor...


    pub_type: 杂志文章


    authors: Hernández HG,Sandoval-Hernández AG,Garrido-Gil P,Labandeira-Garcia JL,Zelaya MV,Bayon GF,Fernández AF,Fraga MF,Arboleda G,Arboleda H

    更新日期:2018-11-01 00:00:00

  • Cell-free circulating epimarks in cancer monitoring.

    abstract::Cancer numbers increasing, cases heterogeneity and the drug resistance emergence have pushed scientists to search for innovative solutions for patients and epimutations can be one. Methylated DNA, modified nucleosomes and noncoding RNAs are found in all cells, including tumor cells. They are intracellular actors but a...


    pub_type: 杂志文章


    authors: Duforestel M,Briand J,Bougras-Cartron G,Heymann D,Frenel JS,Vallette FM,Cartron PF

    更新日期:2020-01-01 00:00:00

  • An update on the epigenetics of psychotic diseases and autism.

    abstract::The examination of potential roles of epigenetic alterations in the pathogenesis of psychotic diseases have become an essential alternative in recent years as genetic studies alone are yet to uncover major gene(s) for psychosis. Here, we describe the current state of knowledge from the gene-specific and genome-wide st...


    pub_type: 杂志文章,评审


    authors: Abdolmaleky HM,Zhou JR,Thiagalingam S

    更新日期:2015-01-01 00:00:00

  • Conference Scene: Epigenetic regulation: from mechanism to intervention.

    abstract::The Medical Research Council Clinical Sciences Centre Symposium on Epigenetic Regulation: From Mechanism to Intervention in London, UK, which was held on 20-22 June 2012, attracted 305 participants from around the globe and included 37 speakers and 85 selected poster presentations. The organizing committee, led by Nia...


    pub_type: 新闻


    authors: Chatterjee A

    更新日期:2012-10-01 00:00:00

  • Gadd45b and N-methyl-D-aspartate induced DNA demethylation in postmitotic neurons.

    abstract:AIM:In nondividing neurons examine the role of Gadd45b in active 5-methylcytosine (5MC) and 5-hydroxymethylcytosine (5HMC) removal at a gene promoter highly implicated in mental illnesses and cognition, Bdnf. MATERIALS & METHODS:Mouse primary cortical neuronal cultures with and without Gadd45b siRNA transfection were ...


    pub_type: 杂志文章


    authors: Gavin DP,Kusumo H,Sharma RP,Guizzetti M,Guidotti A,Pandey SC

    更新日期:2015-01-01 00:00:00

  • Epigenome-wide cross-tissue predictive modeling and comparison of cord blood and placental methylation in a birth cohort.

    abstract:AIM:We compared predictive modeling approaches to estimate placental methylation using cord blood methylation. MATERIALS & METHODS:We performed locus-specific methylation prediction using both linear regression and support vector machine models with 174 matched pairs of 450k arrays. RESULTS:At most CpG sites, both ap...


    pub_type: 杂志文章


    authors: De Carli MM,Baccarelli AA,Trevisi L,Pantic I,Brennan KJ,Hacker MR,Loudon H,Brunst KJ,Wright RO,Wright RJ,Just AC

    更新日期:2017-03-01 00:00:00

  • Sex-specific histone modifications in mouse fetal and neonatal germ cells.

    abstract:AIMS:Epigenetic signatures of germline cells are dynamically reprogrammed to induce appropriate differentiation, development and sex specification. We investigated sex-specific epigenetic changes in mouse fetal germ cells (FGCs) and neonatal germ cells. MATERIALS & METHODS:Six histone marks in mouse E13.5 FGCs and P1 ...


    pub_type: 杂志文章


    authors: Kawabata Y,Kamio A,Jincho Y,Sakashita A,Takashima T,Kobayashi H,Matsui Y,Kono T

    更新日期:2019-04-01 00:00:00

  • Epigenetic diet: impact on the epigenome and cancer.

    abstract::A number of bioactive dietary components are of particular interest in the field of epigenetics. Many of these compounds display anticancer properties and may play a role in cancer prevention. Numerous studies suggest that a number of nutritional compounds have epigenetic targets in cancer cells. Importantly, emerging...


    pub_type: 杂志文章,评审


    authors: Hardy TM,Tollefsbol TO

    更新日期:2011-08-01 00:00:00

  • Single-molecule, genome-scale analyses of DNA modifications: exposing the epigenome with next-generation technologies.

    abstract::DNA modifications represent an integral part of the epigenome and they have a pivotal role in regulation of genome function. Despite the wide variety of analytical techniques that have been developed to detect DNA modifications, their investigation at the single-genome level is only beginning to emerge. In contrast to...


    pub_type: 杂志文章,评审


    authors: Zillner K,Németh A

    更新日期:2012-08-01 00:00:00

  • An OMIC biomarker detection algorithm TriVote and its application in methylomic biomarker detection.

    abstract:AIM:Transcriptomic and methylomic patterns represent two major OMIC data sources impacted by both inheritable genetic information and environmental factors, and have been widely used as disease diagnosis and prognosis biomarkers. MATERIALS & METHODS:Modern transcriptomic and methylomic profiling technologies detect th...


    pub_type: 杂志文章


    authors: Xu C,Liu J,Yang W,Shu Y,Wei Z,Zheng W,Feng X,Zhou F

    更新日期:2018-04-01 00:00:00

  • Epigenetic modulation of autophagy genes linked to diabetic nephropathy by administration of isorhamnetin in Type 2 diabetes mellitus rats.

    abstract::Aim: To assess isorhamnetin efficacy for diabetic kidney disease in a Type 2 diabetes mellitus rat model, through investigating its effect at the epigenetic, mRNA and protein levels. Materials & methods: Type 2 diabetes mellitus was induced in rats by streptozotocin and high-fat diet. Rats were treated with isorhamnet...


    pub_type: 杂志文章


    authors: Matboli M,Ibrahim D,Hasanin AH,Hassan MK,Habib EK,Bekhet MM,Afifi AM,Eissa S

    更新日期:2021-01-07 00:00:00

  • Chromatin response to DNA double-strand break damage.

    abstract::Manipulation of chromatin, in which genomic DNA is packaged, is a fundamental requirement for all DNA-based metabolic processes in eukayotic cells. Histone variant incorporation, histone post-translational modifications, and ATP-dependent chromatin remodeling are three major strategies for chromatin manipulation, and ...


    pub_type: 杂志文章,评审


    authors: Bao Y

    更新日期:2011-06-01 00:00:00

  • Interview with Stephen B Baylin.

    abstract::Stephen B Baylin is a codirector of the Cancer Biology Program at The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins and the Virginia and DK Ludwig Professor of Oncology and Medicine. Baylin attended Duke University, where he earned his medical degree and completed his internship and first year residency i...


    pub_type: 面试


    authors: Baylin SB

    更新日期:2015-01-01 00:00:00

  • DNA methylation and the hygiene hypothesis: connecting respiratory allergy and childhood acute lymphoblastic leukemia.

    abstract::Aim: The hygiene hypothesis states that a lack of infection in early-life suppresses immune system development, and is linked to respiratory allergy (RA) and childhood acute lymphoblastic leukemia (ALL) risk. Little is known about underlying mechanisms, but DNA methylation is altered in RA and ALL, and in response to ...


    pub_type: 杂志文章


    authors: Langie SA,Timms JA,De Boever P,McKay JA

    更新日期:2019-10-01 00:00:00

  • Clinical value and potential mechanisms of LINC00221 in hepatocellular carcinoma based on integrated analysis.

    abstract::This study aimed to unveil the functional roles of LINC00221 in hepatocellular carcinoma (HCC). A discovery cohort and a validation cohort were respectively used to identify and verify the clinical value of LINC00221 in HCC. Bioinformatics analysis was performed to explore its potential mechanisms. LINC00221 was upreg...


    pub_type: 杂志文章


    authors: Feng Y,Dramani Maman ST,Zhu X,Liu X,Bongolo CC,Liang C,Tu J

    更新日期:2021-01-07 00:00:00

  • Estimating cell-type-specific DNA methylation effects in heterogeneous cellular populations.

    abstract::Aim: To develop a method for estimating cell-specific effects in epigenomic association studies in the presence of cell type heterogeneity. Materials & methods: We utilized Monte Carlo Expectation-Maximization (MCEM) algorithm with Metropolis-Hastings sampler to reconstruct the 'missing' cell-specific methylations and...


    pub_type: 杂志文章


    authors: Feng YA,Guo Y,Pain L,Lathrop GM,Laprise C,Moffatt MF,Cookson WO,Liang L

    更新日期:2020-12-22 00:00:00

  • Placental growth factor and Fms related tyrosine kinase-1 are hypomethylated in preeclampsia placentae.

    abstract::Aim: This study aims to examine the DNA methylation (DNAm) and expression patterns of genes associated with placental angiogenesis in preeclampsia. Materials & methods: DNAm and expression were examined in normotensive (n = 100) and preeclampsia (n = 100) women using pyrosequencing and quantitative real-time PCR respe...


    pub_type: 杂志文章


    authors: M Dave K,Kaur L,Randhir KN,Mehendale SS,Sundrani DP,Chandak GR,Joshi SR

    更新日期:2021-01-20 00:00:00

  • From Prader-Willi syndrome to psychosis: translating parent-of-origin effects into schizophrenia research.

    abstract::Prader-Willi syndrome (PWS) is a relatively rare disorder that originates from paternally inherited deletions and maternal disomy (mUPD) within the 15q11-q13 region or alterations in the PWS imprinting center. Evidence is accumulating that mUPD underlies high prevalence of psychosis among PWS patients. Several genes i...


    pub_type: 杂志文章,评审


    authors: Krefft M,Frydecka D,Adamowski T,Misiak B

    更新日期:2014-01-01 00:00:00

  • Inhibitors of protein methyltransferases as chemical tools.

    abstract::Protein methyltransferases (PMT)s play essential roles in many biological processes through methylation of histones and diverse nonhistone substrates. Dysregulation of these enzymes has been implicated in many diseases including cancers. While PMT-associated biology can be probed via genetic perturbation, this approac...


    pub_type: 杂志文章,评审


    authors: Luo M

    更新日期:2015-01-01 00:00:00

  • Macrophage Kdm6b controls the pro-fibrotic transcriptome signature of foam cells.

    abstract:AIM:In order to identify regulators of foam cells, we studied the H3K27 demethylase Kdm6b (also known as Jmjd3), a known regulator of macrophages, in controlling the transcriptional profile of foam cells. MATERIALS & METHODS:Foam cells from Kdm6b-deleted or Kdm6b wild-type mice were isolated and used for RNA-sequencin...


    pub_type: 杂志文章


    authors: Neele AE,Prange KH,Hoeksema MA,van der Velden S,Lucas T,Dimmeler S,Lutgens E,Van den Bossche J,de Winther MP

    更新日期:2017-04-01 00:00:00

  • Long intergenic noncoding RNA 299 methylation in peripheral blood is a biomarker for triple-negative breast cancer.

    abstract:AIM:To identify DNA methylation biomarkers in peripheral blood samples from triple-negative breast cancer (TNBC) patients. MATERIALS & METHODS:We conducted an epigenome-wide association study (EWAS): the most promising markers were identified in 233 TNBC case-control pairs (discovery set) and subsequently validated in...


    pub_type: 杂志文章


    authors: Bermejo JL,Huang G,Manoochehri M,Mesa KG,Schick M,Silos RG,Ko YD,Brüning T,Brauch H,Lo WY,Hoheisel JD,Hamann U

    更新日期:2019-01-01 00:00:00

  • Epigenetic regulation of miRNA genes and their role in human melanomas.

    abstract::Melanoma is a leading cause of death from cancers in the USA. While exposure to UV radiation has long been identified as a primary risk factor for melanoma, molecular mechanisms directly linking UV radiation to the development of melanoma, especially metastatic melanoma, are poorly understood. Besides abnormality in s...


    pub_type: 杂志文章,评审


    authors: Perera RJ,Ray A

    更新日期:2012-02-01 00:00:00

  • Role of histone acetylation in gastric cancer: implications of dietetic compounds and clinical perspectives.

    abstract::Histone modifications regulate the structural status of chromatin and thereby influence the transcriptional status of genes. These processes are controlled by the recruitment of different enzymes to a specific genomic site. Furthermore, obtaining an understanding of these mechanisms could help delineate alternative tr...


    pub_type: 杂志文章,评审


    authors: Calcagno DQ,Wisnieski F,Mota ERDS,Maia de Sousa SB,Costa da Silva JM,Leal MF,Gigek CO,Santos LC,Rasmussen LT,Assumpção PP,Burbano RR,Smith MA

    更新日期:2019-02-01 00:00:00

  • Genetic and epigenetic dysregulation of imprinted genes in the brain.

    abstract::Imprinted genes are an epigenetically regulated class of genes that are preferentially expressed from one parental allele. A number of these genes are crucial for placental function and embryonic growth in mice and humans. Disruption of imprinted genes is also associated with several neurodevelopmental disorders, alth...


    pub_type: 杂志文章,评审


    authors: Kernohan KD,Bérubé NG

    更新日期:2010-12-01 00:00:00

  • Antisense RNAs and epigenetic regulation.

    abstract::Antisense RNA is the first noncoding RNA found to have a regulatory function. With the advances of biological science, it has been recognized that the function of antisense RNAs is not only limited to post-transcriptional regulation, but extends to transcriptional regulation of various important genes leading to epige...


    pub_type: 杂志文章,评审


    authors: Cui I,Cui H

    更新日期:2010-02-01 00:00:00

  • Evidence for type-specific DNA methylation patterns in epilepsy: a discordant monozygotic twin approach.

    abstract::Aim: Epilepsy is a common neurological disorder characterized by recurrent seizures. We performed epigenetic analyses between and within 15 monozygotic (MZ) twin pairs discordant for focal or generalized epilepsy. Methods: DNA methylation analysis was performed using Illumina Infinium MethylationEPIC arrays, in blood ...


    pub_type: 杂志文章


    authors: Mohandas N,Loke YJ,Hopkins S,Mackenzie L,Bennett C,Berkovic SF,Vadlamudi L,Craig JM

    更新日期:2019-06-01 00:00:00

  • Identification of lncRNA-associated ceRNA network in high-grade serous ovarian cancer metastasis.

    abstract::Aim: To uncover a novel lncRNA-miRNA-mRNA network associated with high-grade serous ovarian cancer metastasis. Material & methods: The candidate differentially expressed lncRNAs were obtained from RNA-sequencing data and determined by functional experiments. The downstream miRNAs and mRNAs were identified by bioinform...


    pub_type: 杂志文章


    authors: Li X,Yu S,Yang R,Wang Q,Liu X,Ma M,Li Y,Wu S

    更新日期:2020-07-01 00:00:00