Abstract:
:Aim: To investigate the action mechanism of 1-benzyl-1,4-diazepane (1-BD) as efflux pump inhibitor (EPI) in Escherichia coli mutants: ΔacrAB or overexpressing AcrAB and AcrEF efflux pumps. Materials & methods: Effect of 1-BD on: antibiotic potentiation, by microdilution method; membrane functionality, by fluorimetric assays; ethidium bromide accumulation, by fluorometric real-time efflux assay; AcrB expression, by quantitative photoactivated localization microscopy. Results: 1-BD decreases the minimal inhibitory concentration of levofloxacin and other antibiotics and increase ethidium bromide accumulation in E. coli overexpressing efflux pumps but not in the ΔacrAB strain. 1-BD increases membranes permeability, without sensibly affecting inner membrane polarity and decreases acrAB transcription. Conclusion: 1-BD acts as an EPI in E. coli with a mixed mechanism, different from that of major reference EPIs.
journal_name
Future Microbioljournal_title
Future microbiologyauthors
Casalone E,Vignolini T,Braconi L,Gardini L,Capitanio M,Pavone FS,Dei S,Teodori Edoi
10.2217/fmb-2019-0296subject
Has Abstractpub_date
2020-07-01 00:00:00pages
987-999eissn
1746-0913issn
1746-0921journal_volume
15pub_type
杂志文章abstract::HCV is a major cause of chronic liver disease worldwide. There is no vaccine available and the current antiviral therapies fail to cure approximately half of treated patients. Liver disease caused by HCV infection is the most common indication for orthotopic liver transplantation. Unfortunately, reinfection of the new...
journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/fmb.11.9
更新日期:2011-03-01 00:00:00
abstract:AIM:To investigate the cost of antibiotic resistance versus the potential for resistant clones to adapt in maintaining polymorphism for resistance. MATERIALS & METHODS:Experimental evolution of Escherichia coli carrying different resistance alleles was performed under an environment devoid of antibiotics and evolution...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb.15.61
更新日期:2015-01-01 00:00:00
abstract::Linda Hazlett is a department chair and distinguished professor at Wayne State University (MI, USA). Her research is focused on the host immune response to Pseudomonas aeruginosa and its role in ocular infections. Dr Hazlett has been funded continuously by the NIH by R01 support for 34 years. She is currently principa...
journal_title:Future microbiology
pub_type: 面试
doi:10.2217/fmb.13.49
更新日期:2013-07-01 00:00:00
abstract::This paper analyzes the most recent reports regarding the direct and indirect effects of the 13-valent pneumococcal conjugate vaccine (PCV13) when administered to infants and young children. PCV13 extends the protective effects reported for the 7-valent pneumococcal conjugate vaccine (PCV7). A further reduction in the...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb.15.81
更新日期:2015-01-01 00:00:00
abstract::Most humans are infected with herpes simplex virus (HSV) type 1 in early childhood and remain latently infected throughout life. While most individuals have mild or no symptoms, some will develop destructive HSV keratitis. Ocular infection with HSV-1 and its associated sequelae account for the majority of corneal blin...
journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/fmb.11.73
更新日期:2011-08-01 00:00:00
abstract:AIM:To isolate and characterize the two phenotypically distinct subpopulations from Klebsiella pneumoniae clonal cultures grown in the simulate microgravity environment. MATERIALS & METHODS:Here clonal culture of K. pneumoniae strain ATCC BAA-1705 was grown within a vertically rotating wall vessel bioreactor. Microsco...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb-2017-0032
更新日期:2017-09-01 00:00:00
abstract:AIMS:AFN-1252 is a novel inhibitor of FabI, an essential enzyme in Staphylococcus spp. This study was undertaken to assess the safety, tolerability and pharmacokinetic properties of AFN-1252, following oral administration in an ascending dose trial. MATERIALS & METHODS:This was a double-blind, randomized, placebo-cont...
journal_title:Future microbiology
pub_type: 杂志文章,随机对照试验
doi:10.2217/fmb.15.101
更新日期:2015-01-01 00:00:00
abstract:AIMS:Nosocomial infections cause significant morbidity and mortality in cancer patients. As a result of their debilitated immune system, cancer patients are likely candidates for colonization with anaerobes. We sought to compare the distribution of nosocomial infections in neutropenic and non-neutropenic cancer patient...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb.12.125
更新日期:2012-12-01 00:00:00
abstract:AIM:The primary objective of this study was to evaluate correlations among mortality, intensive care unit (ICU) length of stay and airway microbiotas in septic patients. MATERIALS & METHODS:A deep-sequencing analysis of the 16S rRNA gene V4 region was performed. RESULTS:The nasal microbiota in septic patients was dom...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb-2018-0349
更新日期:2019-03-01 00:00:00
abstract::The past decade has seen a significant increase in the incidence of invasive fungal infections. The antifungal armamentarium for the treatment of serious fungal infections remains limited. A possible approach to overcoming antifungal drug resistance and high mortality rates seen in severe fungal infections is to combi...
journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/17460913.2.2.115
更新日期:2007-04-01 00:00:00
abstract::Background: There is increasing evidence of the association between microbiome dysfunction and Parkinson's disease (PD). Moreover, some PD patients suffer from unintentional weight loss (WL) which may precede the motor manifestations of the disease. Materials & methods: Gut microbiota profiling by 16S rRNA gene sequen...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb-2019-0336
更新日期:2020-09-01 00:00:00
abstract::Candida albicans, a diploid yeast commensal and opportunist pathogen, has evolved unusual mechanisms for maintenance of genetic diversity in the absence of a complete sexual cycle. These include chromosomal polymorphisms, mitotic recombination events, and gains and losses of heterozygosity, superimposed on a fundament...
journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/fmb.09.113
更新日期:2010-01-01 00:00:00
abstract:AIM:Raman spectroscopy is an analytical method with a broad range of applications across multiple scientific fields. We report on a possibility to differentiate between two important Gram-positive species commonly found in clinical material - Staphylococcus aureus and Staphylococcus epidermidis - using this rapid nonin...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb-2016-0224
更新日期:2017-08-01 00:00:00
abstract::Background: A major focus of tuberculosis drug discovery is aimed at the development of novel antibiotics with activity against drug-resistant strains of Mycobacterium tuberculosis. Results: We have synthesized ten isoniazid derivatives and investigated for antibacterial activity toward M. tuberculosis H37Rv and isoni...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb-2019-0085
更新日期:2020-07-01 00:00:00
abstract::Chlamydial infections cause a wide range of acute and chronic diseases. Chlamydia trachomatis is the most common sexually transmitted bacterium while Chlamydia pneumoniae causes infections of the upper and lower respiratory tract. Chlamydia are obligate, intracellular bacteria with a biphasic developmental cycle that ...
journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/fmb.14.13
更新日期:2014-01-01 00:00:00
abstract::Helicobacter pylori is arguably one of the most successful pathogens; it colonizes the stomachs of more than half of the human population. Colonization and persistence in such an inhospitable niche requires the presence of exquisite adaptive mechanisms. One of the proteins that contributes significantly to the remarka...
journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/fmb.13.43
更新日期:2013-06-01 00:00:00
abstract::Organism-wide approaches examining the genetic mechanisms controlling growth and proliferation have proven to be a powerful tool in the study of pathogenic fungi. For many fungal pathogens techniques to study transcription and protein expression are particularly useful, and offer insights into infection processes by t...
journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/fmb.16.9
更新日期:2016-01-01 00:00:00
abstract:AIMS:To investigate whether Cek2 (as Cek1) is involved in the biogenesis of the fungal cell wall and to uncover differences and similitudes between both proteins. MATERIALS & METHODS:We used molecular genetics to characterize the role of Cek2 in MTL-heterozygous cells. RESULTS:Deletion of CEK2 (similar to CEK1) rende...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb.15.127
更新日期:2016-01-01 00:00:00
abstract:AIM:To investigate the role of Candida albicans TUP1-mediated filamentation in the colonization of the mice gut. MATERIALS & METHODS:We used molecular genetics to generate a strain where filamentation is regulated by altering the expression of the TUP1 gene with tetracyclines. RESULTS:The colonization rates reached w...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb-2018-0012
更新日期:2018-06-01 00:00:00
abstract::Genotyping is used to track specific isolates of Mycobacterium tuberculosis in a community. It has been successfully used in epidemiologic research (termed 'molecular epidemiology') to study the transmission dynamics of TB. In this article, we review the genetic markers used in molecular epidemiologic studies includin...
journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/fmb.10.165
更新日期:2011-02-01 00:00:00
abstract:AIM:The aim was to evaluate the in vitro activity of fosfomycin against common uropathogens in a fosfomycin-naive population in India. METHODS:The authors prospectively collected and tested all consecutive isolates of Escherichia coli and Enterococcus spp. from urine samples between December 2009 and April 2010. RESU...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb.13.31
更新日期:2013-05-01 00:00:00
abstract:AIM:To explore the molecular epidemiology and clinical characteristics of Washington University polyomavirus (WUPyV) infection in pediatric patients with acute respiratory tract infections in China. MATERIALS & METHODS:A laboratory surveillance was performed to recruit pediatric patients with acute respiratory tract i...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb-2016-0144
更新日期:2017-05-01 00:00:00
abstract::Salmonella enterica serovars Typhi and Paratyphi are the causative agents of human typhoid fever. Current typhoid vaccines are ineffective and are not widely used in endemic areas. Greater understanding of host-pathogen interactions during Salmonella infection should facilitate the development of improved vaccines to ...
journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/fmb.14.98
更新日期:2015-01-01 00:00:00
abstract::Inhibitors that target the retroviral enzyme reverse transcriptase (RT) have played an indispensable role in the treatment and prevention of HIV-1 infection. They can be grouped into two distinct therapeutic groups, namely the nucleoside and nucleotide RT inhibitors (NRTIs), and the non-nucleoside RT inhibitors (NNRTI...
journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/fmb.15.106
更新日期:2015-01-01 00:00:00
abstract::Malaria remains a major international health challenge. Resistance to a number of existing drugs and evidence of the emergence of artemisinin resistance has emphasized the need for new antimalarials. A new approach has been the preparation of dual-function compounds that include a chloroquine-like antimalarial group a...
journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/fmb.13.18
更新日期:2013-04-01 00:00:00
abstract::In this exclusive interview, Simon Gaisford discusses his current research into gut dysbiosis and its possible relevance to disease states, as well as the efficacy of probiotic supplementation and its possible use as treatment. This interview was conducted by Ellen Colvin, Commissioning Editor of Future Microbiology. ...
journal_title:Future microbiology
pub_type: 面试
doi:10.2217/fmb-2019-0016
更新日期:2019-03-01 00:00:00
abstract::Amoebiasis is a serious infectious disease that is caused by the unicellular parasite, Entamoeba histolytica. This parasite is mainly found in developing countries, and are named owing to its ability to destroy tissues. The molecular mechanisms that regulate the virulence of this parasite are not well understood. In r...
journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/fmb.10.140
更新日期:2010-12-01 00:00:00
abstract::Aim: The primary objective of this study was to evaluate the effects of polypeptide-enriched Gastrodia elata extracts (GE) on vulvovaginal candidiasis (VVC). Materials & methods: A VVC model induced by Candida albicans (C. albicans) infection was successfully developed in BALB/c mice. After treatment, the colony-formi...
journal_title:Future microbiology
pub_type: 杂志文章
doi:10.2217/fmb-2018-0299
更新日期:2019-07-01 00:00:00
abstract::The outstanding ability of Acinetobacter baumannii to cause outbreaks and acquire multidrug resistance motivated the development of a plethora of typing techniques, which can help infection preventionists and hospital epidemiologists to more efficiently implement intervention controls. Nowadays, the world is witnessin...
journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/fmb-2019-0134
更新日期:2019-08-01 00:00:00
abstract::The aggregate of microorganisms residing on the surface of the skin, in the oropharynx and in the GI tract, known as the human microbiota, play a major role as natural reservoirs for bacterial resistance to antibiotics. Fluoroquinolones (FQ) are among the most prescribed antibiotics and a major increase in FQ resistan...
journal_title:Future microbiology
pub_type: 杂志文章,评审
doi:10.2217/fmb.15.40
更新日期:2015-01-01 00:00:00