Differentiation between Staphylococcus aureus and Staphylococcus epidermidis strains using Raman spectroscopy.

Abstract:

AIM:Raman spectroscopy is an analytical method with a broad range of applications across multiple scientific fields. We report on a possibility to differentiate between two important Gram-positive species commonly found in clinical material - Staphylococcus aureus and Staphylococcus epidermidis - using this rapid noninvasive technique. MATERIALS & METHODS:For this, we tested 87 strains, 41 of S. aureus and 46 of S. epidermidis, directly from colonies grown on a Mueller-Hinton agar plate using Raman spectroscopy. DISCUSSION & CONCLUSION:The method paves a way for separation of these two species even on high number of samples and therefore, it can be potentially used in clinical diagnostics.

journal_name

Future Microbiol

journal_title

Future microbiology

authors

Rebrošová K,Šiler M,Samek O,Růžička F,Bernatová S,Ježek J,Zemánek P,Holá V

doi

10.2217/fmb-2016-0224

subject

Has Abstract

pub_date

2017-08-01 00:00:00

pages

881-890

eissn

1746-0913

issn

1746-0921

journal_volume

12

pub_type

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