Complementary roles of the Cek1 and Cek2 MAP kinases in Candida albicans cell-wall biogenesis.

Abstract:

AIMS:To investigate whether Cek2 (as Cek1) is involved in the biogenesis of the fungal cell wall and to uncover differences and similitudes between both proteins. MATERIALS & METHODS:We used molecular genetics to characterize the role of Cek2 in MTL-heterozygous cells. RESULTS:Deletion of CEK2 (similar to CEK1) renders cells sensitive to cell-wall-interfering drugs and, when overexpressed, Cek2 can become phosphorylated upon the same stimuli that activate Cek1. This is dependent on elements of the sterile-vegetative growth (SVG) pathway. Cek2, contrary to Cek1, mediates a transcriptional activity in a C. albicans-adapted two-hybrid system that is essential for Cek1-Cek2 interaction. CONCLUSION:Cek2 has a cryptic role in cell-wall biogenesis and its role is not entirely redundant to Cek1.

journal_name

Future Microbiol

journal_title

Future microbiology

authors

Correia I,Román E,Prieto D,Eisman B,Pla J

doi

10.2217/fmb.15.127

subject

Has Abstract

pub_date

2016-01-01 00:00:00

pages

51-67

issue

1

eissn

1746-0913

issn

1746-0921

journal_volume

11

pub_type

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