Production of phosphinothricin-tolerant rice (Oryza sativa L.) through the application of phosphinothricin as growth regulator.

Abstract:

: A novel procedure has been developed to produce rice (Oryza sativa L.) tolerant to the herbicide phosphinothricin (PPT) by means of in vitro selection. First, sublethal and lethal concentrations of PPT on 7-day-old seedlings were determined and morphogenetic events in response to the PPT treatment evaluated. Differentiation of 6-30 microshoots on 5-40% of the treated plant material was observed on a hormone-free culture medium supplemented with a sublethal concentration of PPT. We proved that PPT is morphogenetically active, similar to the action of many other herbicides, showing cytokinin-like effects in rice tissue culture. Fertile plants were grown from those microshoots having PPT tolerance under greenhouse conditions. To the best of our knowledge, this is the first report on the production of rice plants tolerant to this herbicide without genetic transformation. Since PPT is a competitive inhibitor of glutamine synthetase (GS), total GS activity in PPT-tolerant and PPT-sensitive plants was examined comprehensively in order to decide whether this enzyme has any role in PPT tolerance. An elevated GS activity was detected in PPT-tolerant plant material which could result in an elevated PPT tolerance at unchanged concentrations of the herbicide.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Toldi O,Tóth S,Oreifig AS,Kiss E,Jenes B

doi

10.1007/s002990000276

subject

Has Abstract

pub_date

2000-12-01 00:00:00

pages

1226-1231

issue

12

eissn

0721-7714

issn

1432-203X

pii

10.1007/s002990000276

journal_volume

19

pub_type

杂志文章
  • Genetic transformation of Medicago species by Agrobacterium tumefaciens and electroporation of protoplasts.

    abstract::Shoot and leaf segments of a non-regenerable Medicago sativa L. genotype were cocultivated with the "shooty" mutant of Agrobacterium tumefaciens carrying the pGV 2206 plasmid. Transformed callus lines were selected and regenerated on the hormone free B5 medium. Southern blot analysis demonstrated integration of T-DNA ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269987

    authors: Kuchuk N,Komarnitski I,Shakhovsky A,Gleba Y

    更新日期:1990-04-01 00:00:00

  • Production of gynogenic haploids of Hordeum vulgare L.

    abstract::Haploid plants were regenerated from cultured unfertilized ovaries of Hordeum vulgare L. (barley). Optimal response was obtained by the addition of 0.6 μM 4-chloro-2-methylphenoxyacetic acid (MCPA), 2.8 μM indole-3-acetic acid (IAA) and 4.4 μM 6-benzyladenine (BA) in the N6 medium. Further increase in the rate of call...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00239094

    authors: Castillo AM,Cistué L

    更新日期:1993-01-01 00:00:00

  • Overexpression of wheat dehydrin DHN-5 enhances tolerance to salt and osmotic stress in Arabidopsis thaliana.

    abstract::Late Embryogenesis Abundant (LEA) proteins are associated with tolerance to water-related stress. A wheat (Triticum durum) group 2 LEA proteins, known also as dehydrin (DHN-5), has been previously shown to be induced by salt and abscisic acid (ABA). In this report, we analyze the effect of ectopic expression of Dhn-5 ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-007-0412-x

    authors: Brini F,Hanin M,Lumbreras V,Amara I,Khoudi H,Hassairi A,Pagès M,Masmoudi K

    更新日期:2007-11-01 00:00:00

  • Plant regeneration from protoplasts of wheat (Triticum aestivum cv. Hartog).

    abstract::Morphologically normal green plants have reproducibly been regenerated from protoplasts of an Australian wheat (Triticum aestivum cv. Hartog). The protoplasts were isolated from fine embryogenic suspension cultures which were initiated from embryogenic callus. Protoplasts were incubated in a modified liquid MS medium ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00231832

    authors: He DG,Yang YM,Scott KJ

    更新日期:1992-02-01 00:00:00

  • Tobacco plants perturbed in the ubiquitin-dependent protein degradation system accumulate callose, salicylic acid, and pathogenesis-related protein 1.

    abstract::Transgenic tobacco plants expressing an inhibitor of ubiquitin-dependent protein degradation, ubiquitin variant ubR48, spontaneously formed necrotic lesions and displayed altered responsiveness to tobacco mosaic virus attack. These plants were analyzed for the accumulation of defense-related compounds and the expressi...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s002990050501

    authors: Conrath U,Klessig DF,Bachmair A

    更新日期:1998-08-01 00:00:00

  • Deciphering the physiological and molecular mechanisms for copper tolerance in autotetraploid Arabidopsis.

    abstract:KEY MESSAGE:Autotetraploid Arabidopsis line esd and 4COL exhibit enhanced tolerance to Cu stress by enhancing activation of antioxidative defenses, altering expression of genes related to Cu transport, chelation, and ABA-responsive. Autopolyploidy is ubiquitous among angiosperms and often results in better adaptation t...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-017-2176-2

    authors: Li M,Xu G,Xia X,Wang M,Yin X,Zhang B,Zhang X,Cui Y

    更新日期:2017-10-01 00:00:00

  • Contrasting pattern of somatic and zygotic embryo development in alfalfa (Medicago sativa L.) as revealed by scanning electron microscopy.

    abstract::Scanning electron microscopy has been used to investigate the morphological changes occurring during the development of alfalfa somatic embryos. Embryos were initiated from callus, transferred to suspension culture and matured on solid agar medium. This developmental pattern was compared to that of zygotic embryos dev...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00235082

    authors: Xu N,Bewley JD

    更新日期:1992-06-01 00:00:00

  • A novel WRKY transcriptional factor from Thlaspi caerulescens negatively regulates the osmotic stress tolerance of transgenic tobacco.

    abstract::A novel member of the WRKY gene family, designated TcWRKY53, was isolated from a cadmium (Cd)-treated Thlaspi caerulescens cDNA library by differential screening. WRKY proteins specifically bind to W-boxes, which are found in the promoters of many genes involved in defense and response to environmental stress. TcWRKY5...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-007-0499-0

    authors: Wei W,Zhang Y,Han L,Guan Z,Chai T

    更新日期:2008-04-01 00:00:00

  • Plant regeneration from protoplasts isolated from long-term cell cultures of wheat (Triticum aestivum L.).

    abstract::A friable and fast-growing type of callus was isolated from a long term shoot-competent cell culture of wheat. The suspension cultures established from this callus consisted of small, densely cytoplasmic cells which divided more rapidly but with a lower plant regeneration frequency than the original culture. A high yi...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00231799

    authors: Chang YF,Wang WC,Warfield CY,Nguyen HT,Wong JR

    更新日期:1991-03-01 00:00:00

  • Genotype-specific heat shock proteins in two maize inbreds.

    abstract::Leaf blade tissue of maize inbred lines B73 and Mo17 was analyzed for intraspecific genetic variability in the heat shock response. The maize inbreds were characterized for acquired thermal tolerance and patterns of heat shock protein synthesis. The leakage conductivity assay of membrane stability during stress indica...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00233096

    authors: Jorgensen JA,Weng J,Ho TH,Nguyen HT

    更新日期:1992-10-01 00:00:00

  • Sudan-β-d-glucuronides and their use for the histochemical localization of β-glucuronidase activity in transgenic plants.

    abstract:: Synthesis of five different Sudan-β-D-glucuronides (I, II, III, IV, and RedB) was performed by condensation of a set of red Sudan diazo dyes with methyl (1-deoxy-2,3,4-tri-O-acetyl-1-trichloroacetimidoyl-α-D-glucopyran)uronate. After the acid and alcohol groups had been deprotected, the resulting compounds were used ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s002990000219

    authors: Van der Eycken E,Terryn N,Goeman JL,Carlens G,Nerinckx W,Claeyssens M,Van der Eycken J,Van Montagu M,Brito-Arias M,Engler G

    更新日期:2000-10-01 00:00:00

  • Isolation and culture of protoplasts from high anthocyanin-producing callus of sweet potato.

    abstract::Optimum conditions for the isolation and culture of protoplasts from high anthocyanin-producing callus of sweet potato (Ipomoea batatas) were investigated. Growth phase of callus and the ratio of callus-enzyme solution affected the yield of protoplasts. Composition of the medium and protoplast density were examined fo...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269369

    authors: Nishimaki T,Nozue M

    更新日期:1985-10-01 00:00:00

  • Site-specific recombination for genetic engineering in plants.

    abstract::Site-specific recombination has been developed into a genetic engineering tool for higher eukaryotes. The manipulation of newly introduced DNA is now possible in the course of genetic transformation procedures, thus making the process more predictable and reliable. Also, a wide variety of chromosomal rearrangements us...

    journal_title:Plant cell reports

    pub_type: 杂志文章,评审

    doi:10.1007/s00299-003-0616-7

    authors: Lyznik LA,Gordon-Kamm WJ,Tao Y

    更新日期:2003-06-01 00:00:00

  • Sequential rooting media and rooting capacity of Sequoiadendron giganteum in vitro. Peroxidase activity as a marker.

    abstract::The rooting capacities of tips of seedling, juvenile and mature shoots of Sequoiadendron giganteum were compared on different rooting media (inductive and expressive media) after passage on an elongating medium. None of the cuttings rooted when continuously kept on medium containing the auxin NAA and vitamin D2. Perox...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269555

    authors: Berthon JY,Boyer N,Gaspar T

    更新日期:1987-10-01 00:00:00

  • In vitro propagation ofAtractylodes lancea.

    abstract::Shoot cultures ofAtractylodes lancea DC. (Compositae) have been established by inoculating the flower bud on Linsmaier-Skoog's medium supplemented with 10(-5) M naphthaleneacetic acid and 10(-5) M 6-benzyl-aminopurine. Shoots were multiplied on a medium containing 10(-6) M 6-benzylaminopurine. Propagated shoots rooted...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF02441005

    authors: Hiraoka N,Yamada N,Kodama T,Tomita Y

    更新日期:1984-06-01 00:00:00

  • Enhanced induction of microspore embryogenesis after n-butanol treatment in wheat (Triticum aestivum L.) anther culture.

    abstract::The aim of this study was the improvement of embryo production in wheat anther culture. Three butanol alcohols, n-butanol, sec-butanol and tert-butanol, were evaluated for their effect on microspore embryogenesis in two spring cultivars of wheat, Pavon and Caramba. Application of n-butanol, at 0.1 and 0.2% (v/v) in th...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-007-0500-y

    authors: Soriano M,Cistué L,Castillo AM

    更新日期:2008-05-01 00:00:00

  • Organogenesis of stem and leaf protoplasts of a haploid golden delicious apple clone (Malus Xdomestica Borkh.).

    abstract::Highly viable protoplasts were isolated in large numbers from in vitro-grown leaf and stem tissues of a haploid clone of the apple scion cultivar Golden Delicious (Malus Xdomestica Borkh.). Protoplasts from both sources divided rapidly to give microcallus, when cultured in a modified Kao and Michayluk-based medium. Fo...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00241946

    authors: Patat-Ochatt EM,Boccon-Gibod J,Duron M,Ochatt SJ

    更新日期:1993-01-01 00:00:00

  • A Streptomyces chitosanase is active in transgenic tobacco.

    abstract::Growth inhibition towards Rhizopus nigricans, Fusarium oxysporum f. sp. radicis-lycopersici, Verticillium albo-atrum and Pythium ultimum was observed in vitro using a purified chitosanase from an actinomycete, Streptomyces sp, strain N174. The corresponding gene, with its own signal peptide, was inserted into pBI121.7...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00193724

    authors: El Quakfaoui S,Potvin C,Brzezinski R,Asselin A

    更新日期:1995-12-01 00:00:00

  • Long-term optimized embryogenic cultures in durum wheat (Triticum durum Desf.).

    abstract::Five varieties of durum wheat: Appulo, Ofanto, Latino, Creso, and Castello (Triticum durum Desf.) adapted to the semi-arid mediterranean environment have been tested for their in vitro response. Compact, embryogenic, highly regenerable calli originated from primary callus derived through proliferation of the scutellum...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00193145

    authors: Borrelli GM,Lupotto E,Locatelli F,Wittmer G

    更新日期:1991-09-01 00:00:00

  • Influence of light intensity and selection scheme on regeneration time of transgenic flax plants.

    abstract::This study aimed at establishing a protocol to increase the number of regenerated shoots and to limit the recovery of "escapes" during the regeneration of transgenic flax plants (cv Barbara). Here, we describe how light, adapted media and selection scheme could stimulate the transformation process, the organogenic pot...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-008-0638-2

    authors: Caillot S,Rosiau E,Laplace C,Thomasset B

    更新日期:2009-03-01 00:00:00

  • Rice and chickpea GDPDs are preferentially influenced by low phosphate and CaGDPD1 encodes an active glycerophosphodiester phosphodiesterase enzyme.

    abstract:KEY MESSAGE:Rice and chickpea GDPD s are transcriptionally influenced by mineral deficiencies; especially, by phosphate starvation and CaGDP1 encodes an active glycerophosphodiester phosphodiesterase enzyme. Glycerophosphodiester phosphodiesterases (GDPDs) are enzymes involved in the degradation of glycerophosphodieste...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-016-1984-0

    authors: Mehra P,Giri J

    更新日期:2016-08-01 00:00:00

  • The promoter of the AlSAP gene from the halophyte grass Aeluropus littoralis directs a stress-inducible expression pattern in transgenic rice plants.

    abstract:KEY MESSAGE:When fused to " Pr AlSAP " promoter, transcripts of gusA exhibited similar accumulation patterns in transgenic rice as AlSAP transcripts in A. littoralis. Pr AlSAP can be used for engineering abiotic stress tolerance. We previously showed that ectopic expression of a stress-associated protein gene from Aelu...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-015-1825-6

    authors: Ben-Saad R,Meynard D,Ben-Romdhane W,Mieulet D,Verdeil JL,Al-Doss A,Guiderdoni E,Hassairi A

    更新日期:2015-10-01 00:00:00

  • Genetic modification in Malaysia and India: current regulatory framework and the special case of non-transformative RNAi in agriculture.

    abstract::Recent developments in modern biotechnology such as the use of RNA interference (RNAi) have broadened the scope of crop genetic modification. RNAi strategies have led to significant achievements in crop protection against biotic and abiotic stresses, modification of plant traits, and yield improvement. As RNAi-derived...

    journal_title:Plant cell reports

    pub_type: 杂志文章,评审

    doi:10.1007/s00299-019-02446-6

    authors: Darsan Singh JK,Mat Jalaluddin NS,Sanan-Mishra N,Harikrishna JA

    更新日期:2019-12-01 00:00:00

  • Early nodulin 93 protein gene: essential for induction of somatic embryogenesis in oil palm.

    abstract:KEY MESSAGE:Transcript profiling during the early induction phase of oil palm tissue culture and RNAi studies in a model somatic embryogenesis system showed that EgENOD93 expression is essential for somatic embryogenesis. Micropropagation of oil palm through tissue culture is vital for the generation of superior and un...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-020-02571-7

    authors: Chan PL,Rose RJ,Abdul Murad AM,Zainal Z,Ong PW,Ooi LC,Low EL,Ishak Z,Yahya S,Song Y,Singh R

    更新日期:2020-11-01 00:00:00

  • Synthesis of an HIV-1 Tat transduction domain-rotavirus enterotoxin fusion protein in transgenic potato.

    abstract::A DNA fragment encoding a 12-amino acid (aa) HIV-1 Tat transduction peptide fused to a 90-aa murine rotavirus NSP4 enterotoxin protein (Tat-NSP4(90)) was transferred to Solanum tuberosum by Agrobacterium tumefaciens-mediated transformation. The fusion gene was detected in the genomic DNA of transformed plant leaf tiss...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-003-0697-3

    authors: Kim TG,Langridge WH

    更新日期:2004-01-01 00:00:00

  • Improvement of Aconitum napellus micropropagation by liquid culture on floating membrane rafts.

    abstract::An efficient method was developed using floating membrane rafts (Liferaft(™)) for the micropropagation of Aconitum napellus (Ranunculaceae), a cut flower crop with a low natural propagation rate. This was achieved by introducing shoot tips into culture on Murashige and Skoog's (1962) solid medium, or liquid medium-sup...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00238594

    authors: Watad AA,Kochba M,Nissim A,Gaba V

    更新日期:1995-03-01 00:00:00

  • Production of marker-free disease-resistant potato using isopentenyl transferase gene as a positive selection marker.

    abstract::The use of antibiotic or herbicide resistant genes as selection markers for production of transgenic plants and their continuous presence in the final transgenics has been a serious problem for their public acceptance and commercialization. MAT (multi-auto-transformation) vector system has been one of the different st...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-010-0974-x

    authors: Khan RS,Ntui VO,Chin DP,Nakamura I,Mii M

    更新日期:2011-04-01 00:00:00

  • Agrobacterium rhizogenes-mediated transformation of Echinacea purpurea.

    abstract::Echinacea purpurea seedlings were inoculated with several Agrobacterium rhizogenes strains in order to obtain hairy roots. Infection with A. rhizogenes strains LMG63 and LMG150 resulted in callus formation. Upon infection with strains ATCC 15834 and R1601 hairy roots were obtained. Opine detection confirmed transforma...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00236463

    authors: Trypsteen M,Van Lijsebettens M,Van Severen R,Van Montagu M

    更新日期:1991-06-01 00:00:00

  • The salicylic acid-induced protection of non-climacteric unripe pepper fruit against Colletotrichum gloeosporioides is similar to the resistance of ripe fruit.

    abstract::The anthracnose fungus Colletotrichum gloeosporioides deleteriously affects unripe pepper fruit, but not ripe fruit. Here, we show that the induction of local acquired resistance (LAR) by salicylic acid (SA), 2,6-dichloroisonicotinic acid, or benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester pretreatment pro...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-009-0756-5

    authors: Lee S,Hong JC,Jeon WB,Chung YS,Sung S,Choi D,Joung YH,Oh BJ

    更新日期:2009-10-01 00:00:00

  • Transcriptome and phytohormone analysis reveals a comprehensive phytohormone and pathogen defence response in pear self-/cross-pollination.

    abstract:KEY MESSAGE:Candidate genes were identified and the role of phytohormones such as JA-Me and ABA in the synthesis of S-RNase was emphasized in pear self-incompatibility. Self-incompatibility (SI) occurs widely in flowering plants as an intraspecific reproductive barrier. This phenomenon promotes variation within species...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-017-2194-0

    authors: Shi D,Tang C,Wang R,Gu C,Wu X,Hu S,Jiao J,Zhang S

    更新日期:2017-11-01 00:00:00