Abstract：
:A friable and fast-growing type of callus was isolated from a long term shoot-competent cell culture of wheat. The suspension cultures established from this callus consisted of small, densely cytoplasmic cells which divided more rapidly but with a lower plant regeneration frequency than the original culture. A high yield of protoplasts was released from suspension cells (2 to 3×10(7) protoplasts per ml packed cell volume) when treated with enzyme mixtures. The isolated protoplasts divided at a relatively high frequency (20% to 50%) in both liquid and agarose-solidified KM8p medium. Up to 0.21% of the dividing protoplasts continued to divide and form micro-calli. Sixty-eight plants were regenerated from micro-calli, and among the 30 plants which were transplanted to the greenhouse, 3 have survived.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Chang YF,Wang WC,Warfield CY,Nguyen HT,Wong JRdoi
10.1007/BF00231799subject
Has Abstractpub_date
1991-03-01 00:00:00pages
611-4issue
11eissn
0721-7714issn
1432-203Xjournal_volume
9pub_type
杂志文章abstract：:Donor plants of Hordeum vulgare L. cv. Igri were grown in a conditioned environment to minimise fluctuations in the composition of the microspore population. After isolation different types of microspores were identified within each population, amongst others an embryogenic subpopulation. It was shown that the optimum...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233415
更新日期：1993-10-01 00:00:00
abstract：:A method for regenerating pigeonpea [Cajanus cajan (L.) Millsp.] plants has been developed using distal cotyledonary segments of mature seeds as explants. A large number of shoot buds were induced directly from explants of genotypes T-15-15 and GAUT-82-90 when cultured on six different basal media fortified with 22.2 ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050469
更新日期：1998-06-01 00:00:00
abstract：KEY MESSAGE:Transgenic rice overexpressing PLCP attenuated the virulence of Xanthomonas oryzae pv. oryzae through extensive activation of transduction signal and transcription activities that orchestrate downstream responses including the biosynthesis of secondary metabolites and up-regulation of several pathogenesis-r...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-019-02502-1
更新日期：2020-04-01 00:00:00
abstract：:An efficient plant regeneration system from leaf-derived callus in 6 genotypes of Avena sativa L. has been established. Regenerable callus was induced in the basal 1-2 mm region of 2 to 5 day old seedlings. Plants were regenerated from the regenerable callus and grown to maturity. The frequency of regenerable callus f...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00238604
更新日期：1995-03-01 00:00:00
abstract：: Cells of a suspension culture of Doritaenopsis cv. New Toyohashi were placed in a mixture of 2 M glycerol and 0.4 M sucrose for 15 min at room temperature and then dehydrated with a vitrification solution (PVS2) for 1-3 h on ice and plunged into liquid nitrogen. The highest viability (64% by 2,3,5-triphenyltetrazoliu...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990000255
更新日期：2000-12-01 00:00:00
abstract：KEY MESSAGE:High and low resveratrol (Res) contents in two cultivars are correlated with the expression abundance of Myb14 , which could directly activate transcriptional expression of stilbene synthase gene ( STS ). Resveratrol (3,5,4'-trihydroxystilbene) is one of the natural polyphenols produced by secondary metabol...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1642-3
更新日期：2014-10-01 00:00:00
abstract：:It has been reported that long term cell cultures from maize endosperms are not completely de-differentiated, maintaining some tissue-specific synthesis. We analyzed the expression of zein (the major storage protein of maize seeds) in cultures derived from wildtype and opaque-2 maize endosperms. In wildtype cultures, ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00272049
更新日期：1989-03-01 00:00:00
abstract：:The voltage-dependent anion channels (VDACs), mitochondrial outer membrane components, are present in organisms from fungi to animals and plants. They are thought to function in the regulation of metabolite transport between mitochondria and the cytoplasm. Sufficient knowledge on plant VDACs has been accumulated, so t...
journal_title：Plant cell reports
pub_type： 杂志文章,评审
doi：10.1007/s00299-009-0741-z
更新日期：2009-09-01 00:00:00
abstract：KEY MESSAGE:Candidate genes were identified and the role of phytohormones such as JA-Me and ABA in the synthesis of S-RNase was emphasized in pear self-incompatibility. Self-incompatibility (SI) occurs widely in flowering plants as an intraspecific reproductive barrier. This phenomenon promotes variation within species...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-017-2194-0
更新日期：2017-11-01 00:00:00
abstract：:The gene coding for the green fluorescent protein (GFP) from jellyfish was introduced into conifer tissues by microprojectile bombardment and its transient expression was detected. Two versions of the GFP gene, wild-type GFP and modified GFP with a cryptic intron removed, were directly compared for their expression in...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF01088278
更新日期：1997-02-01 00:00:00
abstract：KEY MESSAGE:Transcript profiling during the early induction phase of oil palm tissue culture and RNAi studies in a model somatic embryogenesis system showed that EgENOD93 expression is essential for somatic embryogenesis. Micropropagation of oil palm through tissue culture is vital for the generation of superior and un...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-020-02571-7
更新日期：2020-11-01 00:00:00
abstract：:Late Embryogenesis Abundant (LEA) proteins are associated with tolerance to water-related stress. A wheat (Triticum durum) group 2 LEA proteins, known also as dehydrin (DHN-5), has been previously shown to be induced by salt and abscisic acid (ABA). In this report, we analyze the effect of ectopic expression of Dhn-5 ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-007-0412-x
更新日期：2007-11-01 00:00:00
abstract：:Essential oils were produced by flowers of Hyacinthus orientalis L. that had been regenerated in vitro. The production of these oils was affected by the concentration of gibberellic acid and sucrose in the medium and by temperature. The highest concentration of essential oils was obtained when regenerated flowers were...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00231941
更新日期：1995-06-01 00:00:00
abstract：:We report the production of taxadiene by transformation of N. benthamiana with a taxadiene synthase gene. The production was significantly increased by an elicitor treatment or metabolic pathway shunting. Paclitaxel (Taxol(®)) was first isolated from the bark of the pacific yew tree as an anticancer agent and has been...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1568-9
更新日期：2014-06-01 00:00:00
abstract：KEY MESSAGE:Cadmium sensitivity in sultr1;1 - sultr1;2 double mutant with limiting sulfate supply is attributed to the decreased glutathione content that affected oxidative defense but not phytochelatins' synthesis. In plants, glutathione (GSH) homeostasis plays pivotal role in cadmium (Cd) detoxification. GSH is synth...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-015-1892-8
更新日期：2016-02-01 00:00:00
abstract：:Citrus psorosis is a serious viral disease affecting citrus trees in many countries. Its causal agent is Citrus psorosis virus (CPsV), the type member of genus Ophiovirus. CPsV infects most important citrus varieties, including oranges, mandarins and grapefruits, as well as hybrids and citrus relatives used as rootsto...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-007-0422-8
更新日期：2008-01-01 00:00:00
abstract：:A genetic transformation protocol for green ash (Fraxinus pennsylvanica) hypocotyl explants was developed. Green ash hypocotyls were transformed using Agrobacterium tumefaciens strain EHA105 harboring binary vector pq35GR containing the neomycin phosphotransferase (nptII) and beta-glucuronidase (GUS) fusion gene, and ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-009-0697-z
更新日期：2009-06-01 00:00:00
abstract：:Protoplasts were isolated from seedling roots, hypocotyls, and cotyledons of four cultivars of Helianthus annuus and from leaves of axenic shoot cultures of the wild species H. praecox, H. scaberimus and H. rigidus. Optimal culture conditions were established for the respective protoplast systems, using the agarose be...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269815
更新日期：1986-08-01 00:00:00
abstract：:Green plants were repeatedly regenerated from suspension-derived protoplasts of Kentucky blue grass (Poa pratensis L.) cv. "Geronimo". One suspension was capable of donating competent protoplasts during long-term culture i. e. 10-16 months after its establishment. The plating efficiency of the protoplasts from three d...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233055
更新日期：1993-08-01 00:00:00
abstract：:Rosmarinic acid (RA) is a natural antioxidant produced by cell suspension cultures of sage (Salvia officinalis L.). The growth and production of RA by these cells can be modified by the type of culture medium. Production can be increased 10-fold to attain 6.4 g.1(-1) under optimal conditions. Investigation of kinetics...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232160
更新日期：1992-04-01 00:00:00
abstract：KEY MESSAGE:Cowpea miRNAs and Argonaute genes showed differential expression patterns in response to CPSMV challenge Several biotic stresses affect cowpea production and yield. CPSMV stands out for causing severe negative impacts on cowpea. Plants have two main induced immune systems. In the basal system (PTI, PAMP-tri...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-020-02548-6
更新日期：2020-08-01 00:00:00
abstract：:A new method for obtaining transgenic cowpea was developed using positive selection based on the Escherichia coli 6-phosphomannose isomerase gene as the selectable marker and mannose as the selective agent. Only transformed cells were capable of utilizing mannose as a carbon source. Cotyledonary node explants from 4-d...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-012-1230-3
更新日期：2012-06-01 00:00:00
abstract：: Identifying transformed plant lines carrying the antibiotic resistance marker gene, neomycin phosphotransferase II, requires a more definitive test than the ability of the plant to grow on kanamycin. Although a number of alternative assays have been described, most are cumbersome, time consuming and/or require the us...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050014
更新日期：2000-01-01 00:00:00
abstract：KEY MESSAGE:We developed transgenic sweet potato with Spomin (sucrose-inducible minimal promoter)-GUS gene-fused constructs. Induced GUS activities by Spomin were higher than those by CaMV 35S promoter. We developed transgenic sweet potato (Ipomoea batatas L. Lam. cv. Kokei no. 14) plants with Spomin (sucrose-inducible...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-019-02453-7
更新日期：2019-11-01 00:00:00
abstract：:Protocols were developed for regeneration and Agrobacterium-mediated transformation of Actinidia eriantha Benth. A. eriantha has a number of features that make it a useful tool for functional genomics in Actinidia: the vines are relatively small and non-vigorous in nature, flowers form all over the vine including on l...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0080-7
更新日期：2006-05-01 00:00:00
abstract：:An efficient micropropagation system for Saussurea involucrata Kar. et Kir., an endangered Chinese medicinal plant, has been developed. Shoot organogenesis occurred from S. involucrata leaf explants inoculated on medium with appropriate supplements of plant growth regulators. 66.0% of shoot regeneration frequency and ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0230-6
更新日期：2007-03-01 00:00:00
abstract：:Accurate and timely detection of transgene copy number in sugarcane is currently hampered by the requirement to use Southern blotting, needing relatively large amounts of genomic DNA and, therefore, the continued growth and maintenance of bulky plants in containment glasshouses. In addition, the sugarcane genome is bo...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-011-1150-7
更新日期：2012-01-01 00:00:00
abstract：:Cell-wall invertase (CIN) catalyzes the hydrolysis of sucrose into glucose and fructose for the supply of carbohydrates to sink organs via an apoplastic pathway. To study the CIN genes in rice (Oryza sativa L.), we isolated cDNA clones showing amino acid similarity to the plant cell wall invertase proteins from a sear...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-004-0910-z
更新日期：2005-06-01 00:00:00
abstract：: Somatic embryogenesis and whole plant regeneration were achieved in callus cultures derived from immature zygotic embryos of Acacia mangium. Embryogenic callus was induced on MS medium containing combinations of TDZ (1-2 mg/l), IAA (0.25-2 mg/l) and a mixture of amino acids. Globular embryos developed on embryogenic ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990000288
更新日期：2001-01-01 00:00:00
abstract：:Fertile transgenic barley (Hordeum vulgare L.) plants were obtained by high velocity particle bombardment. The plasmid pBCl was used to deliver the selectable hph gene and reporter Gus gene into immature embryo. After the selection culture 18 hygromycin resistant plants were obtained. Samples for Southern hybridizatio...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232038
更新日期：1995-02-01 00:00:00
