Abstract：
:Fertile transgenic barley (Hordeum vulgare L.) plants were obtained by high velocity particle bombardment. The plasmid pBCl was used to deliver the selectable hph gene and reporter Gus gene into immature embryo. After the selection culture 18 hygromycin resistant plants were obtained. Samples for Southern hybridization and enzymatic Gus assay were obtained from 11 plants. Southern hybridization confirmed the presence of the hph gene in the 11 hygromycin resistant plants(T0). Enzymatic assay indicated that all the t0 plants that showed hph positive in Southern analysis possessed detectable amount of Gus activity. To date all the 11 t0 plants reached maturity and mature seeds were obtained Transmission of the hph gene to progeny(T1) of two independent t0 plants was confirmed by Southern hybridization.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Hagio T,Hirabayashi T,Machii H,Tomotsune Hdoi
10.1007/BF00232038subject
Has Abstractpub_date
1995-02-01 00:00:00pages
329-34issue
5eissn
0721-7714issn
1432-203Xjournal_volume
14pub_type
杂志文章abstract：KEY MESSAGE:Effective microspore embryogenesis in triticale is determined by a specific hormonal homeostasis: low value of IAA/cytokinins, IAA/ABA and cytokinins/ABA ratios as well as proper endogenous/exogenous auxin balance, which favours androgenic structure formation and green plant regeneration ability. The concen...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1686-4
更新日期：2015-01-01 00:00:00
abstract：: A novel procedure has been developed to produce rice (Oryza sativa L.) tolerant to the herbicide phosphinothricin (PPT) by means of in vitro selection. First, sublethal and lethal concentrations of PPT on 7-day-old seedlings were determined and morphogenetic events in response to the PPT treatment evaluated. Differen...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990000276
更新日期：2000-12-01 00:00:00
abstract：:A DNA fragment encoding a 12-amino acid (aa) HIV-1 Tat transduction peptide fused to a 90-aa murine rotavirus NSP4 enterotoxin protein (Tat-NSP4(90)) was transferred to Solanum tuberosum by Agrobacterium tumefaciens-mediated transformation. The fusion gene was detected in the genomic DNA of transformed plant leaf tiss...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-003-0697-3
更新日期：2004-01-01 00:00:00
abstract：:Quaternary benzophenanthridine alkaloids (sanguinarine, chelerythrine, chelirubine, chelilutine and macarpine) are specifically induced by cell wall components of Penicillium and Saccharomyces in a colorless strain of Eschscholtzia californica cell suspension cultures. Classical elicitors such as the Phytophthora mega...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00272770
更新日期：1987-12-01 00:00:00
abstract：:A biolistic transformation procedure was used to transform embryogenic Pinus radiata tissue with constructs containing the Zea mays UBI1 (ubiquitin)-promoter followed by the P. radiata CAD (cinnamyl alcohol dehydrogenase) cDNA in sense or anti-sense orientation or in the form of an inverted-repeat. The effect of the d...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-004-0911-y
更新日期：2005-05-01 00:00:00
abstract：:A genetic transformation protocol for green ash (Fraxinus pennsylvanica) hypocotyl explants was developed. Green ash hypocotyls were transformed using Agrobacterium tumefaciens strain EHA105 harboring binary vector pq35GR containing the neomycin phosphotransferase (nptII) and beta-glucuronidase (GUS) fusion gene, and ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-009-0697-z
更新日期：2009-06-01 00:00:00
abstract：:Callus cultures of Vitis labruscana cv. Concord were initiated from both immature fruit and petiole tissue. Analysis revealed that the acid hydrolyzable precursors to β-damascenone, a compound important to the flavor of most grape species, were present in both callus types. Light and increased sucrose concentration we...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00231971
更新日期：1994-05-01 00:00:00
abstract：:Randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were used to analyse the genetic diversity of Portuguese Prunus dulcis cultivars and their relationship to important foreign cultivars. Of the primers tested, 6 (out of 60) RAPD and 5 (out of 18) ISSR primers were selected for th...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-003-0659-9
更新日期：2003-08-01 00:00:00
abstract：:Conditions necessary for the isolation and culture of protoplasts from suspension cultures of sugar, fodder and garden beets were investigated. Good yields of protoplasts were obtained by treating cells with a mixture of cellulase, Macerozyme and Driselase enzymes. Nutritional requirements of beet protoplasts were fou...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269896
更新日期：1985-12-01 00:00:00
abstract：:A reliable plant regeneration system is described for the production of adventitious shoots from root explants of spinach. Explants from roots of axenic shoots and roots induced on cultured hypocotyl explants were used for adventitious shoot induction. Explants from apical, middle and basal root regions were incubated...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050359
更新日期：1997-12-01 00:00:00
abstract：:Six sugarbeet (Beta vulgaris L.) lines (GWI-248, SPB-11, MonoHy 55, SMS-1, EL45 and FC607) were tested for regeneration. Shoot cultures were initiated in vitro from naked, sterilized embryos obtained from mature seed. Excised petioles from cultured shoots were plated on Gamborg's B5 medium and four modified Murashige ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00272972
更新日期：1988-01-01 00:00:00
abstract：KEY MESSAGE:By comparing series full-length cDNA libraries stressed and control, the dynamic process of salt stress response in Upland cotton was studied, and reactive oxygen species and gibberellins signaling pathways were proposed. The Upland cotton is the most important fiber plant with highly salt tolerance. Howeve...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-015-1761-5
更新日期：2015-06-01 00:00:00
abstract：:The sequence of events in the functional body pattern formation during the somatic embryo development in cowpea suspensions is described under three heads. Early stages of somatic embryogenesis were characterized by both periclinal and anti-clinal cell divisions. Differentiation of the protoderm cell layer by periclin...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0966-4
更新日期：2005-11-01 00:00:00
abstract：: This study was conducted to detect the optimum conditions for DNA transfer into mature embryos of barley via electroporation. Cultured mature embryos of barley were directly electroporated in the presence of the pBI 121 vector carrying both the β-glucuronidase (GUS) and neomycin phosphotransferase II (npt II) genes. ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002999900182
更新日期：2000-07-01 00:00:00
abstract：:Essential oils were produced by flowers of Hyacinthus orientalis L. that had been regenerated in vitro. The production of these oils was affected by the concentration of gibberellic acid and sucrose in the medium and by temperature. The highest concentration of essential oils was obtained when regenerated flowers were...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00231941
更新日期：1995-06-01 00:00:00
abstract：:A protocol is described for rapid and large-scale propagation of the woody aromatic and medicinal shrub Vitex negundo by in vitro culture of nodal segments from mature plants. Of the three different cytokinins - N6-benzyladenine (BA), kinetin, and thidiazuron - evaluated as supplements to Murashige and Skoog (MS) medi...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050576
更新日期：1998-12-01 00:00:00
abstract：:Cell suspension cultures of cowpea (Vigna sp.) were able to produce extracellular peroxidase. Different growth regulator concentrations induced different peroxidase activity in callus. The crude extracellular medium after four weeks of culture showed higher (6 times) specific peroxidase activity and higher thermo stab...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232092
更新日期：1990-07-01 00:00:00
abstract：KEY MESSAGE:This study established a rapid method for the gene expression analysis in potato tubers. The use of microtubers would be useful for primary evaluation of tuber-expressed genes. In the development of transgenic potato or of potato with other genome modifications (e.g., genome editing or RNA-directed DNA meth...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-020-02572-6
更新日期：2020-11-01 00:00:00
abstract：:Putrescine, spermidine, and spermine levels during somatic embryogenesis of interior spruce (Picea glauca x Picea engelmannii complex) were quantified On abscisic acid supplemented growth medium putrescine and spermidine levels increased two-fold coinciding with maturation of the early somatic embryos to globular embr...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232981
更新日期：1996-03-01 00:00:00
abstract：:A new freezer stock of an Agrobacterium tumefaciens clone of LBA4404/pBI121, designated 8999, was found to contain a mutation in the T-DNA region. GUS activity in Agrobacterium 8999 was reduced to levels in negative controls of LBA4404. Additionally, GUS activity in T1 seedlings from tobacco plants transformed with 89...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232066
更新日期：1996-02-01 00:00:00
abstract：KEY MESSAGE:A chalcone synthase gene ( PaCHS ) was isolated and functionally characterized from liverwort. The ectopic expression of PaCHS in Marchantia paleacea callus raised the flavonoids content. Chalcone synthase (CHS; EC 2.3.1.74) is pivotal for the biosynthesis of flavonoid and anthocyanin pigments in plants. It...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1702-8
更新日期：2015-02-01 00:00:00
abstract：:A NaCl-tolerant callus line of Cicer arietinum has been isolated, as a spontaneous variant, on agar-solidified MS-medium supplemented with 100 mM NaCl. The growth of this line, in the presence of 100 mM NaCl, was comparable to that of the NaCl-sensitive callus line growing in the absence of NaCl. Regarding relative to...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00270968
更新日期：1984-04-01 00:00:00
abstract：:The rooting capacities of tips of seedling, juvenile and mature shoots of Sequoiadendron giganteum were compared on different rooting media (inductive and expressive media) after passage on an elongating medium. None of the cuttings rooted when continuously kept on medium containing the auxin NAA and vitamin D2. Perox...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269555
更新日期：1987-10-01 00:00:00
abstract：:Citrus psorosis is a serious viral disease affecting citrus trees in many countries. Its causal agent is Citrus psorosis virus (CPsV), the type member of genus Ophiovirus. CPsV infects most important citrus varieties, including oranges, mandarins and grapefruits, as well as hybrids and citrus relatives used as rootsto...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-007-0422-8
更新日期：2008-01-01 00:00:00
abstract：UNLABELLED:Musa acuminata ssp. burmannica, one of the wild progenitors contributing 'A genome' to the present-day dessert bananas, has a long evolutionary history intervened by human activities. In this study, ISSR markers were used to analyze the pattern of genetic variation and differentiation in 32 individuals along...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-012-1273-5
更新日期：2012-09-01 00:00:00
abstract：KEY MESSAGE:We describe the potato CDPK family and place StCDPK7 as a player in potato response to Phytophthora infestans infection, identifying phenylalanine ammonia lyase as its specific phosphorylation target in vitro. Calcium-dependent protein kinases (CDPKs) decode calcium (Ca2+) signals and activate different sig...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-017-2144-x
更新日期：2017-07-01 00:00:00
abstract：KEY MESSAGE:The Arabidopsis AP2/ERF family transcription factor AtERF15 is nuclear-localized and positively regulates ABA and stress responses. Abscisic acid (ABA) is a major plant hormone that controls the expression of hundreds genes involved in various aspects of plant growth and development, such as seed developmen...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1688-2
更新日期：2015-01-01 00:00:00
abstract：:An efficient culture system has been developed for repeated cycles of somatic embryogenesis in microspore-derived embryos of Brassica juncea without a callus phase. Haploid embryos produced through anther culture showed a high propensity for direct production of somatic embryos in response to 2 mgL(-1) BA and 0.1 mgL(...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00237138
更新日期：1993-03-01 00:00:00
abstract：:Plant regeneration from immature embryos of peanut (Arachis hypogaea L.) can be accomplished through somatic embryogenesis. The highest frequency of somatic embryo formation occurred on B5 medium plus 0.5-1.0 mg/l picloram. Shoots and plants developed from the somatic embryos only after extended culture on basal mediu...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00778536
更新日期：1989-04-01 00:00:00
abstract：:Cultivation of Catharanthus roseus hairy root cultures at different temperatures was found to have an effect on growth rate and indole alkaloid content as well as lipid composition. When lowering the temperature, the roots responded by increasing the degree of unsaturation of cellular lipids, which was mainly due to a...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00234368
更新日期：1992-07-01 00:00:00
