Abstract：
:A DNA fragment encoding a 12-amino acid (aa) HIV-1 Tat transduction peptide fused to a 90-aa murine rotavirus NSP4 enterotoxin protein (Tat-NSP4(90)) was transferred to Solanum tuberosum by Agrobacterium tumefaciens-mediated transformation. The fusion gene was detected in the genomic DNA of transformed plant leaf tissues by PCR DNA amplification. The Tat-NSP4(90 )fusion protein was identified in transformed tuber extracts by immunoblot analysis using anti-NSP4(90) and anti-Tat as the primary antibodies. Enzyme-linked immunosorbent assay results showed that the Tat-NSP4(90) fusion protein made up to 0.0015% of the total soluble tuber protein. The synthesis of Tat-NSP4(90) fusion protein in transformed potato tuber tissues demonstrates the feasibility of plant cell delivery of the HIV-1 Tat transduction domain as a carrier for non-specific targeting of fused antigens to the mucosal immune system.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Kim TG,Langridge WHdoi
10.1007/s00299-003-0697-3keywords:
subject
Has Abstractpub_date
2004-01-01 00:00:00pages
382-7issue
6eissn
0721-7714issn
1432-203Xjournal_volume
22pub_type
杂志文章abstract：:Embryogenic callus and suspension cultures of carrot (Daucus carota L., cv. Nantaise), growing on/in medium including 1 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D), were transferred to medium with or without this plant growth regulator, to impair or induce, respectively, further development of somatic embryos. The en...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-004-0869-9
更新日期：2005-01-01 00:00:00
abstract：:Exposure of Arabidopsis thaliana L. to an acclimation temperature (+4°C) results in a rapid increase of frost tolerance from -3°C to -7°C. This increase could be correlated to changes in soluble protein pattern. Analysis of in vitro translation products from isolated mRNA suggests that induction acts at the transcript...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00272740
更新日期：1988-12-01 00:00:00
abstract：:The effect of the polyamine spermidine on the growth of crown gall tumors was determined using the potato disc bioassay. Addition of lmM spermidine resulted in a 30-50% increase in tumor growth. The spermidine effect was found to be biphasic, with lmM being optimal. Closely related polyamines including spermine, as we...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269212
更新日期：1985-04-01 00:00:00
abstract：KEY MESSAGE:A homologous gene of MPEC from Phyllostachys edulis was isolated and characterized. Its overexpression in Arabidopsis thaliana increased chlorophyll concentration and photosynthesis efficiency, indicating it is involved in chlorophyll biosynthesis. Magnesium-protoporphyrin IX monomethyl ester cyclase (MPEC)...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-015-1846-1
更新日期：2015-11-01 00:00:00
abstract：:The goal of the study was to characterize the optimal developmental stage of zygotic embryo expiants of the hardwood forest tree species yellow-poplar (Liriodendron tulipifera L.) for the initiation of embryogenic cultures, using morphological measurements and polypeptide profiles of the embryos. Developing zygotic em...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00234289
更新日期：1991-07-01 00:00:00
abstract：:Quantitative real-time polymerase chain reaction (PCR) assays were designed that enabled the zygosity of transgenes in soybean [Glycine max (L.) Merrill] and peanut (Arachis hypogaea L.) to be determined. The two zygosity assays, based on TaqMan technology that uses a fluorogenic probe which hybridizes to a PCR target...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990100326
更新日期：2001-07-01 00:00:00
abstract：:Approximately 10(6) protoplast-derived cell colonies of sainfoin were stressed with streptomycin and two resistant colonies were recovered. Plants regenerated from these colonies could be recallused on streptomycin-containing medium three years after growth in the absence of the antibiotic.Ultrastructural studies show...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269636
更新日期：1986-12-01 00:00:00
abstract：:A T-DNA insertional mutant OsPE of rice gives twin and triplet seedlings in up to 20 % of the seeds. Detailed cytological and histological analysis of OsPE indicated normal male and female gametogenesis in the OsPE mutant. Confocal laser scanning microscopic (CLSM) analysis of the developing seeds of OsPE showed multi...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-012-1291-3
更新日期：2012-10-01 00:00:00
abstract：KEY MESSAGE:The gene and cDNA sequence encoding PAL from Chinese medicinal plant Rhus chinensis were cloned and analyzed, furthermore the biochemical properties, kinetic parameters, differential expression and key sites were studied. Rhus chinensis is a well-known Chinese medicinal plant. Phenylalanine ammonia-lyase (P...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-013-1413-6
更新日期：2013-08-01 00:00:00
abstract：:Quantitative trait loci (QTLs) associated with androgenic responsiveness in triticale were analyzed using a population of 90 DH lines derived from the F1 cross between inbred line 'Saka 3006' and cv. 'Modus', which was used in a number of earlier studies on molecular mapping in this crop. Using Windows QTL Cartographe...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-012-1320-2
更新日期：2012-11-01 00:00:00
abstract：:Accurate and timely detection of transgene copy number in sugarcane is currently hampered by the requirement to use Southern blotting, needing relatively large amounts of genomic DNA and, therefore, the continued growth and maintenance of bulky plants in containment glasshouses. In addition, the sugarcane genome is bo...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-011-1150-7
更新日期：2012-01-01 00:00:00
abstract：:Sweet cherry shows S-RNase-based gametophytic self-incompatibility, which prevents self- and cross-fertilization between genetically related individuals. The specificity of the self-incompatible reaction is determined by two genes located in the S-locus. These encode a pistil-expressed ribonuclease (S-RNase) that inhi...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-010-0857-1
更新日期：2010-07-01 00:00:00
abstract：KEY MESSAGE:The novel sunflower gene HaGLP1 is the first germin-like protein characterized from the family Asteraceae. It alters the host redox status and confers protection against Sclerotinia sclerotiorum and Rhizoctonia solani. Germin-like proteins (GLPs) are a large, diverse and ubiquitous family of plant glycoprot...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-015-1819-4
更新日期：2015-10-01 00:00:00
abstract：:The full-length cDNAs of eight S ribonucleases (S-RNases) were cloned from stylar RNA of European pear cultivars that could not be characterized by the cleaved amplified polymorphic sequences (CAPS) marker system for genotyping European pear cultivars harboring nine S alleles Sa, Sb, Sd, Se, Sh, Sk, Sl, Sq, and Sr. Co...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0254-y
更新日期：2007-03-01 00:00:00
abstract：:An excellent new medium was developed for the production of shikonin derivatives by suspension cultures of Lithospermum erythrorhizon. We investigated the effects of all the components of White's medium on the production of these derivatives. Nitrate, phosphate, copper, sulfate and sucrose had especially marked effect...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269273
更新日期：1981-12-01 00:00:00
abstract：KEY MESSAGE:We demonstrate the role of DREB1A transcription factor in better root and shoot partitioning and higher transpiration efficiency in transgenic chickpea under drought stress Chickpea (Cicer arietinum L.) is mostly exposed to terminal drought stress which adversely influences its yield. Development of cultiva...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1699-z
更新日期：2015-02-01 00:00:00
abstract：:The hybridity of eleven somatic hybrids between a diploidS. tuberosum and a diploidS. phureja clone could be verified because the parent karyotypes differed in their C-banding patterns. The hybrids were hypotetraploid and some carried structurally rearranged chromosomes and/or minute centric fragments. The nucleolar c...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00716844
更新日期：1989-02-01 00:00:00
abstract：KEY MESSAGE:This work provides a detailed histological analysis of the development of Jatropha curcas seeds, together with an assessment of the role of programmed cell death in this process. Seeds of Jatropha curcas are a potential source of raw material for the production of biodiesel, but very little is known about h...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-017-2184-2
更新日期：2017-11-01 00:00:00
abstract：:Reliable regeneration protocols for Eucalyptus camaldulensis using leaf explants from in vitro-grown plants have been developed. Out of the 24 clones tested 13 were regenerated and of these, 6 showed regeneration from more than 60% of the explants. Identical protocols were also successful in the regeneration of some c...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050321
更新日期：1997-09-01 00:00:00
abstract：:Four diverse strains of Agrobacterium tumefaciens (C58, Ach5, GV3111, and A281) were capable of inducing tumors at a high frequency on inoculated stems of lentil (Lens culinaris Medik. cultivar Laird) in vivo, and on excised shoot apices in vitro. GV3111 and Ach5 produced the largest and heaviest tumors in vivo, while...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00234579
更新日期：1991-12-01 00:00:00
abstract：: An Agrobacterium tumefaciens-mediated transformation method has been developed for onions (Allium cepa L.) using immature embryos as the explant source. Transgenic plants were recovered from the open-pollinated onion cultivar Canterbury Longkeeper at a maximum transformation frequency from immature embryos of 2.7%. T...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050743
更新日期：2000-03-01 00:00:00
abstract：:Expiants of five plant species (Allium cepa, Antirrhinum majus, Brassica campestris. Glycine max, and Nicotiana tabacum) were co-cultivated with three Agrobacterium tumefaciens strains under different conditions to assess the effects of acetosyringone and medium pH on strain virulence. Tumours were incited on all dico...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00235354
更新日期：1991-04-01 00:00:00
abstract：:Responses induced by Cd exposure were assessed in tobacco seedlings (Nicotiana tabacum L.) using macro and molecular indices. The 100 microM of Cd exposure reduced the total dry weight and chlorophyll index of the seedlings as much as the genuine Fe-deficiency. Concentration of Fe in the shoots decreased, whereas that...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0092-3
更新日期：2006-04-01 00:00:00
abstract：:A protocol was developed for biolistic transformation of hybrid bermudagrass cv. TifEagle using the bar gene. TifEagle is an ultradwarf used exclusively on golf greens. Herbicide resistance should serve as a useful management tool, especially if methyl-bromide is unavailable for fumigation prior to plant establishment...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-003-0736-0
更新日期：2004-03-01 00:00:00
abstract：KEY MESSAGE:The oil palm EgPAL1 gene promoter and its regulatory region were functional as a promoter in the heterologous system of Arabidopsis according to the cis-acting elements present in that region. The promoter was developmentally regulated, vascular tissue specific and responsive to water stress agents. Phenyla...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-017-2228-7
更新日期：2018-02-01 00:00:00
abstract：:Diarrheal diseases caused by Vibrio cholerae and enterotoxigenic Escherichia coli (ETEC) are worldwide health problems that might be prevented with vaccines based on edible plants expressing the B subunit from either the cholera toxin (CTB) or the E. coli heat labile toxin (LTB). In this work we analyzed the immunity ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-007-0439-z
更新日期：2008-01-01 00:00:00
abstract：:A cDNA clone encoding an ADP-ribosylation factor from potato (Solanum tuberosum L.) was isolated. The nucleotide and deduced amino acid sequences show high homology to known ADP-ribosylation factor sequences from Arabidopsis, yeast, cow and man. In northern blot experiments, all tissues analysed showed expression of t...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233786
更新日期：1994-12-01 00:00:00
abstract：KEY MESSAGE:Overexpressing TaUb2 promoted stem growth and resulted in early flowering in transgenic tobacco plants. Ubiquitin are involved in the production, metabolism and proper function of gibberellin. The ubiquitin-26S proteasome system (UPS), in which ubiquitin (Ub) functions as a marker, is a post-translational r...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-012-1331-z
更新日期：2012-12-01 00:00:00
abstract：:Sugarcane cell suspensions were initiated from leaf callus and sub-cultured every 7 to 10 days by alternate transfer to MS based medium with 3.0 and 1.0 mg 1(-1)2,4-D. Suspensions older than 3 months gave the most reproducible yields of protoplasts. Isolated protoplasts required 50 mM Ca(2+) in the washing solution an...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269934
更新日期：1988-08-01 00:00:00
abstract：:Grapefruit (Citrus paradisi) tissue cultures were examined for qualitative and quantitative changes in flavanone-neohesperidoside content during somatic embryogenesis. Embryos cultured in vitro contain naringin and a rhamnosyl-transferase activity which is capable of rhamnosylating position 2 on the flavanone glucosid...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00270076
更新日期：1989-10-01 00:00:00
