Abstract：
:Sugarcane cell suspensions were initiated from leaf callus and sub-cultured every 7 to 10 days by alternate transfer to MS based medium with 3.0 and 1.0 mg 1(-1)2,4-D. Suspensions older than 3 months gave the most reproducible yields of protoplasts. Isolated protoplasts required 50 mM Ca(2+) in the washing solution and 100 mM Ca(2+) in the culture medium to prevent lysis. At plating densities of 2.0-3.0×10(5) ml(-1), 18% or more of the isolated protoplasts produced cell colonies when cultured in droplets or sectors of Kao and Michayluk (1975) based medium with 1.2% w/v Sea Plaque agarose. Cell colonies were of two morphological types. Those consisting of small, tightly packed cells developed into morphogenic callus. The latter produced an abundance of green meristems from which shoots and whole plants were regenerated.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Chen WH,Davey MR,Power JB,Cocking ECdoi
10.1007/BF00269934subject
Has Abstractpub_date
1988-08-01 00:00:00pages
344-7issue
5eissn
0721-7714issn
1432-203Xjournal_volume
7pub_type
杂志文章abstract：:A novel genic male sterile (GMS) line in Brassica napus L., which was identified in 1999, was found to be controlled by a monogenic dominant gene, which we have designated as MDGMS. The microspores of the MDGMS abort before the degradation of the tapetal cell layer. The F1 fertility from any fertile lines crossed with...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0276-5
更新日期：2007-05-01 00:00:00
abstract：:Murashige and Skoog's modified medium in 1% Difco Bacto-agar supplemented with sugar alcohols (sorbsitol, mannitol), growth regulators (1-naphthalenacetic acid, 2,4-dichlorophenoxyacetic acid, benzyladenine, kinetin) and sugars (fructose, glucose, sucrose) induced aposporous gametophytes from pinnae of Pteris vittata ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232608
更新日期：1991-10-01 00:00:00
abstract：:The non-yellowing gene (NYE1), initially identified from Arabidopsis, is a key regulatory gene responsible for chlorophyll degradation during senescence. Here, FaNYE1, an orthologue of AtNYE1, was further identified from a major type of cool-season turf grass, tall fescue (Festuca arundinacea Schreb.), by RACE-PCR. It...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-011-1028-8
更新日期：2011-07-01 00:00:00
abstract：:The growth and sweet diterpene glucosides of Stevia plants propagated by stem-tip cultures were compared with those of the control plants propagated by seeds. There was no significant difference between the two groups both in growth and in chemical composition. As for the contents of sweet diterpene glucosides, howeve...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00270194
更新日期：1984-10-01 00:00:00
abstract：:Protoplasts isolated from mesophyll cells of Eruca sativa Lam., cultured on suitable medium, underwent sustained cell divisions to form calli. The plating efficiency was found to be 0.4%. The protoplast-derived calli subsequently produced plantlets through organogenesis (15.71%) and somatic embryogenesis (11.25%). Reg...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00272790
更新日期：1987-12-01 00:00:00
abstract：:Ma bamboo (Dendrocalamus latiflorus Munro) belongs to Dendrocalamus genus, Bambusease tribe, Bambusoideae subfamily, Poaceae family. It is a representative species of clumping bamboo, and a principal commercial species for various construction purposes using mature culms and for human consumption using young shoots. A...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-011-1112-0
更新日期：2011-11-01 00:00:00
abstract：KEY MESSAGE:Thirty genes involved in GA and ABA metabolism and signalling were identified, and the expression profiles indicated that they play crucial roles in the bud activity-dormancy transition in tea plants. Gibberellin (GA) and abscisic acid (ABA) are fundamental phytohormones that extensively regulate plant grow...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-017-2238-5
更新日期：2018-03-01 00:00:00
abstract：:MicroRNAs (miRNAs) are small non-coding RNAs that play vital roles in plant abiotic stress responses via cleavage or translational inhibition of their target mRNAs. Populus euphratica is a typical stress-resistant sessile organism that grows in desert areas. Here, we identified sequences of 12 miRNA precursors from 11...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-011-1096-9
更新日期：2011-10-01 00:00:00
abstract：KEY MESSAGE:Marker-free transgenic plants can be generated with high efficiency by using the Cre/ lox P self-excision system controlled by the pollen- and embryo-specific Arabidopsis DLL promoter. In this work, we aimed to study the feasibility of using the pollen- and embryo-specific DLL promoter of the At4g16160 gene...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1726-0
更新日期：2015-03-01 00:00:00
abstract：:This study aimed at establishing a protocol to increase the number of regenerated shoots and to limit the recovery of "escapes" during the regeneration of transgenic flax plants (cv Barbara). Here, we describe how light, adapted media and selection scheme could stimulate the transformation process, the organogenic pot...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-008-0638-2
更新日期：2009-03-01 00:00:00
abstract：:KEY MESSAGE : Rooting of Artemisia annua increases trichome size on leaves and helps drive the final steps of the biosynthesis of the sesquiterpene antimalarial drug, artemisinin. ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-012-1355-4
更新日期：2013-02-01 00:00:00
abstract：:Yucca valida is an important potential source of steroidal saponins closely related to Yucca schidigera, the species that is commercially exploited from the wild as a source of steroidal extracts. Neither of the species has been domesticated mainly because of their slow growth and long life span before harvesting. Her...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0144-3
更新日期：2006-08-01 00:00:00
abstract：:The ability to germinate orchids from seeds in vitro presents a useful and viable method for the propagation of valuable germplasm, maintaining the genetic heterogeneity inherent in seeds. Given the ornamental and medicinal importance of many species within the genus Dendrobium, this review explores in vitro technique...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-015-1829-2
更新日期：2015-10-01 00:00:00
abstract：:Cultured Catharanthus roseus cells exhibit transmembrane ferricyanide reduction through a plasma membrane redox system which may be associated with proton translocation. Evidence shows that endogenous pyridine nucleotides serve as hydrogen donors for the reaction. The proton translocating function of the redox system ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269886
更新日期：1985-12-01 00:00:00
abstract：:Plant meiotic prophase I is a complicated process involving the late zygotene and pachytene stages, both crucial for completing synapsis and recombination. Using David Lily (Lilium davidii var. Willmottiae) as our research material, we performed suppression subtractive hybridization to construct EST library of anthers...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-011-1106-y
更新日期：2011-10-01 00:00:00
abstract：KEY MESSAGE:Several hypomethylated sites within the Karma region of EgDEF1 and hotspot regions in chromosomes 1, 2, 3, and 5 may be associated with mantling. One of the main challenges faced by the oil palm industry is fruit abnormalities, such as the "mantled" phenotype that can lead to reduced yields. This clonal abn...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-020-02561-9
更新日期：2020-09-01 00:00:00
abstract：:Chimeric constructs consisting of the gus coding region fused downstream of promoterun-translated leader sequences from the tobacco osmotin and PR-S genes, the potato proteinase inhibitor 2 gene (pin2), and the cauliflower mosaic virus (CaMV) 35S promoter were biolistically transferred into sugarbeet suspension cells....
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233152
更新日期：1996-08-01 00:00:00
abstract：:Plant regeneration in Arachis pintoi was obtained via two developmental pathways: organogenesis and somatic embryogenesis. Organogenic callus cultures were initiated from pieces of leaf on MS medium supplemented with NAA or 2,4-D in combination with BA, KIN or 2iP. The most suitable combination for plant regeneration ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990000198
更新日期：2000-09-01 00:00:00
abstract：:The microprojectile bombardment of immature embryos has proven to be effective in transforming many indica rice varieties. One of the drawbacks of using immature embryos is the requirement of a large number of high quality immature embryos, which itself is a tedious and laborious process. To circumvent these problems,...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232364
更新日期：1996-01-01 00:00:00
abstract：:Accurate and timely detection of transgene copy number in sugarcane is currently hampered by the requirement to use Southern blotting, needing relatively large amounts of genomic DNA and, therefore, the continued growth and maintenance of bulky plants in containment glasshouses. In addition, the sugarcane genome is bo...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-011-1150-7
更新日期：2012-01-01 00:00:00
abstract：:To develop an Agrobacterium mediated transformation protocol for chrysanthemum we studied the transformation efficiency of commonly used A.tumefaciens strains on 14 genotypes by comparing tumour size and frequency. One genotype was analyzed in detail using 14 strains of both A.tumefaciens and A.rhizogenes. Only a few ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232106
更新日期：1991-01-01 00:00:00
abstract：:Carotenoid cleavage dioxygenases (CCDs) are involved in the production of diverse apocarotenoids including phytohormones, the visual molecules and the aromatic volatile compounds derived from carotenoids. Here, we examined the spatial expression of four of the CCD genes (AtCcd1, 4, 7 and 8) among the nine members of t...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-010-0999-1
更新日期：2011-06-01 00:00:00
abstract：:Green plants were repeatedly regenerated from suspension-derived protoplasts of Kentucky blue grass (Poa pratensis L.) cv. "Geronimo". One suspension was capable of donating competent protoplasts during long-term culture i. e. 10-16 months after its establishment. The plating efficiency of the protoplasts from three d...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233055
更新日期：1993-08-01 00:00:00
abstract：KEY MESSAGE:The radish WRKY gene family was genome-widely identified and played critical roles in response to multiple abiotic stresses. The WRKY is among the largest transcription factors (TFs) associated with multiple biological activities for plant survival, including control response mechanisms against abiotic stre...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-017-2190-4
更新日期：2017-11-01 00:00:00
abstract：KEY MESSAGE:We developed a method for detection of mutants in a large number of plants, and found this method to be applicable to detection of a mutant allele at a concentration of 1/1000. Many techniques for SNP analysis have been developed, but most of these techniques are not so sensitive to be used for detection of...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-018-2274-9
更新日期：2018-06-01 00:00:00
abstract：:Vitrification is a technically simple method for cryopreserving plant germplasm, requiring only the application of suitable cryoprotectants and rapid cooling rates. Sweetpotato (Ipomoea batatas [L.] Lam.) shoot tips obtained from in vitro plants survived liquid nitrogen (-196°C) exposure following a vitrification-indu...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232527
更新日期：1992-05-01 00:00:00
abstract：:A reliable and efficient system for transformation and regeneration of 'Chardonnay' (Vitis vinifera L.) plants via microprojectile bombardment was developed. Improvements over the previous biolistic transformation system included: (1) the use of gold particles for bombardment; (2) step-wise selection at 10 then 15 mg/...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-003-0682-x
更新日期：2003-11-01 00:00:00
abstract：:The direct induction of adventitious shoots from leaf explants obtained from adult plants of Zhanhua winter jujube, an elite variety of Zizyphus jujuba Mill., is reported. The percentage of leaf explants producing shoots and the average number of shoots per explant were significantly improved when 10-day-old leaves we...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0920-5
更新日期：2005-03-01 00:00:00
abstract：:Intertribal somatic hybrids between Brassica napus (2n = 38, AACC) and a dye and medicinal plant Isatis indigotica (2n = 14, II) were obtained by fusions of mesophyll protoplasts. From a total of 237 calli, only one symmetric hybrid (S2) and five asymmetric hybrids (As1, As4, As6, As7 and As12) were established in the...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-009-0712-4
更新日期：2009-07-01 00:00:00
abstract：KEY MESSAGE:Wheat MAPK gene TaMPK14 is N starvation response and is crucial in modulating plant low-N stress tolerance. Improving plant N use efficiency (NUE) contributes largely to the sustainable crop production worldwide. In this study, TaMPK14, a mitogen-activated protein kinase (MAPK) family gene in T. aestivum, w...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-020-02564-6
更新日期：2020-10-01 00:00:00
