Abstract:
:Plant regeneration in Arachis pintoi was obtained via two developmental pathways: organogenesis and somatic embryogenesis. Organogenic callus cultures were initiated from pieces of leaf on MS medium supplemented with NAA or 2,4-D in combination with BA, KIN or 2iP. The most suitable combination for plant regeneration through organogenesis was an initial medium composed of 10 mg/l NAA+1 mg/l BA followed by transfer of the callus to a shoot induction medium (MS+1 mg/l BA). Rooting of regenerated shoots was readily achieved by culture on MS+0.01 mg/l NAA. Embryogenic callus cultures were initiated from pieces of leaf on MS medium supplemented with PICL in combination with KIN, ZEA, BA or 2iP, and the most suitable combinations were 20 mg/l PICL+1 mg/l BA or 2iP. When pieces of embryogenic callus were subcultured on MS+1 mg/l BA, somatic embryos were differentiated and developed further into well-developed plants in MS+1 g/l AC followed by MS medium devoid of plant growth regulators.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Rey HY,Scocchi AM,Gonzalez AM,Mroginski LAdoi
10.1007/s002990000198subject
Has Abstractpub_date
2000-09-01 00:00:00pages
856-862issue
9eissn
0721-7714issn
1432-203Xpii
10.1007/s002990000198journal_volume
19pub_type
杂志文章abstract::The human Bcl-xL gene was transformed into peanut cultivar Georgia Green via microprojectile bombardment. Following selection on hygromycin-containing medium and regeneration, eighty hygromycin-resistant callus clusters were recovered. Southern blot analysis of ten fertile lines revealed multiple insertions of the Bcl...
journal_title:Plant cell reports
pub_type: 杂志文章
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journal_title:Plant cell reports
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journal_title:Plant cell reports
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journal_title:Plant cell reports
pub_type: 杂志文章
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更新日期:1993-01-01 00:00:00
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journal_title:Plant cell reports
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pub_type: 杂志文章,评审
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journal_title:Plant cell reports
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更新日期:2011-08-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1752-6
更新日期:2015-06-01 00:00:00
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更新日期:2018-12-01 00:00:00
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更新日期:2013-12-01 00:00:00
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journal_title:Plant cell reports
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更新日期:2013-11-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章,评审
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更新日期:2018-10-01 00:00:00
abstract::DNA transfer into zygotic embryos of Eucalyptus globulus by microprojectile bombardment was studied with two devices: a gunpowder apparatus and a compressed-helium system. Using, as a test, the transient expression of a reporter gene, we optimized the physical and biological conditions of bombardment. Six-day-old cult...
journal_title:Plant cell reports
pub_type: 杂志文章
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更新日期:1995-07-01 00:00:00
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journal_title:Plant cell reports
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更新日期:1996-11-01 00:00:00