Plant regeneration in Arachis pintoi (Leguminosae) through leaf culture.

Abstract:

:Plant regeneration in Arachis pintoi was obtained via two developmental pathways: organogenesis and somatic embryogenesis. Organogenic callus cultures were initiated from pieces of leaf on MS medium supplemented with NAA or 2,4-D in combination with BA, KIN or 2iP. The most suitable combination for plant regeneration through organogenesis was an initial medium composed of 10 mg/l NAA+1 mg/l BA followed by transfer of the callus to a shoot induction medium (MS+1 mg/l BA). Rooting of regenerated shoots was readily achieved by culture on MS+0.01 mg/l NAA. Embryogenic callus cultures were initiated from pieces of leaf on MS medium supplemented with PICL in combination with KIN, ZEA, BA or 2iP, and the most suitable combinations were 20 mg/l PICL+1 mg/l BA or 2iP. When pieces of embryogenic callus were subcultured on MS+1 mg/l BA, somatic embryos were differentiated and developed further into well-developed plants in MS+1 g/l AC followed by MS medium devoid of plant growth regulators.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Rey HY,Scocchi AM,Gonzalez AM,Mroginski LA

doi

10.1007/s002990000198

subject

Has Abstract

pub_date

2000-09-01 00:00:00

pages

856-862

issue

9

eissn

0721-7714

issn

1432-203X

pii

10.1007/s002990000198

journal_volume

19

pub_type

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