Separation of root nodule cells and identification of tissue-specific genes.

Abstract:

:The interior tissues of the alfalfa (Medicago sativa) root nodule differ in form and function from the peripheral layers. The interior tissues are specialized for the fixation of nitrogen in cells infected by rhizobia. In contrast, the peripheral nodule tissues perform roles that assist the interior tissues: they provide metabolic support and protect the interior tissues from damaging levels of oxygen. We used a novel microdissection technique to separate these tissue types, allowing immunological and molecular comparison between the nodule interior and periphery. Using differential mRNA display reverse transcription and polymerase chain reaction, we compared the mRNA profiles of the separated tissues and identified a transcript specific to the nodule interior, and several peripheral-specific candidate genes.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Ganter G,Duquette M,Dunn K

doi

10.1007/s002990000216

subject

Has Abstract

pub_date

2000-09-01 00:00:00

pages

921-925

issue

9

eissn

0721-7714

issn

1432-203X

pii

10.1007/s002990000216

journal_volume

19

pub_type

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