Abstract:
:Cotyledon explants from zygotic embryos of Panax ginseng produced somatic embryos on Murashige and Skoog basal medium without growth regulators. Somatic embryos developed directly from epidermal cells at the cotyledon base. Somatic embryos were always formed from the side of the cotyledon opposite to the one attached to the medium surface regardless of cotyledon orientation. The frequency of somatic embryo formation from the abaxial epidermis (66%) was much higher than that from the adaxial epidermis (12%). Differences in embryogenic response were likely related to cell structure. Abaxial epidermal cells were filled with reserve materials (lipid bodies), while adaxial epidermal cells were devoid of any prominent reserves. During germination, the reserve materials in the cells of the cotyledons disappeared rapidly. At the same time, the competency of somatic embryo formation from cotyledon explants declined rapidly to zero. Upon culture of the cotyledon explants (for somatic embryo induction), lipid bodies slowly disappeared, but starch grains accumulated prominently. Reserve materials disappeared after commencement of embryogenic cell division. During germination, lipid bodies rapidly disappeared, and chloroplasts developed instead of starch grains.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Choi YE,Yang DC,Kim HS,Choi KTdoi
10.1007/s002990050331subject
Has Abstractpub_date
1997-10-01 00:00:00pages
841-846issue
12eissn
0721-7714issn
1432-203Xpii
10.1007/s002990050331journal_volume
16pub_type
杂志文章abstract:KEY MESSAGE:The first Good Manufacturing Practices production of a purification-free rice-based oral cholera vaccine (MucoRice-CTB) from transgenic plants in a closed cultivation system yielded a product meeting regulatory requirements. Despite our knowledge of their advantages, plant-based vaccines remain unavailable ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1911-9
更新日期:2016-03-01 00:00:00
abstract::Oil palm is a major economic crop for Malaysia. The major challenges faced by the industry are labor shortage, availability of arable land and unstable commodity price. This has caused the industry to diversify its applications into higher value products besides increasing its yield. While conventional breeding has it...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-014-1722-4
更新日期:2015-04-01 00:00:00
abstract::The effect of various inhibitors on differentiation (shoot morphogenesis) in callus cultures of Brassica, Datura and Nicotiana has been investigated. Hormone medium without any inhibitor (control), resulted in 6% shoot formation. Addition of inhibitors such as actinomycin D, cordycepin, abscisic acid, trigonelline and...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00270062
更新日期:1990-03-01 00:00:00
abstract:KEY MESSAGE:Marker-free transgenic plants can be generated with high efficiency by using the Cre/ lox P self-excision system controlled by the pollen- and embryo-specific Arabidopsis DLL promoter. In this work, we aimed to study the feasibility of using the pollen- and embryo-specific DLL promoter of the At4g16160 gene...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1726-0
更新日期:2015-03-01 00:00:00
abstract:KEY MESSAGE:Induced mutations in the waxy locus in rice endosperm did not abolish GBSS activity completely. Compensatory mechanisms in endosperm and leaves caused a major reprogramming of the starch biosynthetic machinery. The mutation of genes in the starch biosynthesis pathway has a profound effect on starch quality ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-019-02388-z
更新日期:2019-03-01 00:00:00
abstract::Globe artichoke represents a natural source of phenolic compounds with dicaffeoylquinic acids along with their biosynthetic precursor chlorogenic acid (5-caffeoylquinic acid) as the predominant molecules. We report the isolation and characterization of a full-length cDNA and promoter of a globe artichoke p-coumaroyl e...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-009-0695-1
更新日期:2009-06-01 00:00:00
abstract::Shoot buds of ginger were successfully encapsulated in 4% sodium alginate gel. Encapsulated buds were germinated in vitro to form roots and shoots. In vitro germination (emergence of sprouts) of encapsulated buds ranged from 16.7% to 81.8% on different media after 5 weeks of incubation. Normal plantlets with an averag...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233325
更新日期:1994-02-01 00:00:00
abstract::TransgenicRhododendron plants were obtained byAgrobacterium tumefaciens-mediated gene transfer.A. tumefaciens harboring a binary vector that contained the chimeric neomycin phosphotransferase II (NPTII) and (3-glucuronidase (GUS) genes was co-cultivated with stem and leaf segments fromRhododendron tissues culturedin v...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01275445
更新日期:1996-11-01 00:00:00
abstract:KEY MESSAGE:MicroRNAs have higher expression stability than protein-coding genes in B. napus seeds and are therefore good reference genes for miRNA and mRNA RT-qPCR analysis. Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) has become the "gold standard" to gain insight into function of ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1773-1
更新日期:2015-07-01 00:00:00
abstract::The efficacy of benzyladenine (BA) to induce multiple shoots from seed explants of chickpea (Cicer arietinum L.) was assessed. Shoot differentiation was influenced by the type of seed explant, genotype and concentration of BA. Orientation of the explant also strongly influenced the shoot regeneration response. The opt...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01142325
更新日期:1997-05-01 00:00:00
abstract::Rapid clonal propagation in three stolon bearing species of Nephrolepis was achieved by tissue culture methods. Explants from young stolons were grown on B5 (Gamborg et al. 1968) medium supplemented with indolebutyric acid where they produced several leaf primordia. These leaf primordia were isolated and grown into en...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272634
更新日期:1982-12-01 00:00:00
abstract::A cDNA clone encoding a putative RTE1-like protein (Rh-RTH1) was obtained from total RNA isolated from senescing rose (Rosa hybrida cv. Tineke) petals using RT-PCR and RACE techniques. The cDNA (1,061 bp) contained an open reading frame of 684 bp corresponding to 227 amino acids. The amino acid sequence had 60.0, 49.6...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0875-z
更新日期:2010-08-01 00:00:00
abstract::We have assessed the capacity of cultured protoplasts from two tissue sources of several commercially-grown broccoli cultivars to regenerate plants. A procedure that employs hypocotyl protoplasts and a culture medium with a high NAA:2,4-D auxin ratio was developed. The procedure permits highly efficient formation of c...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232858
更新日期:1990-10-01 00:00:00
abstract:KEY MESSAGE:Galactoglucomannan oligosaccharides seem to interact with auxin in xylogenic cell culture, thus influencing mainly metaxylem-like tracheary element differentiation depending on timing with hormones and the process kinetics. Complex mapping of Zinnia mesophyll cell transdifferentiation into tracheary element...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-012-1379-9
更新日期:2013-04-01 00:00:00
abstract:KEY MESSAGE:The gene and cDNA sequence encoding PAL from Chinese medicinal plant Rhus chinensis were cloned and analyzed, furthermore the biochemical properties, kinetic parameters, differential expression and key sites were studied. Rhus chinensis is a well-known Chinese medicinal plant. Phenylalanine ammonia-lyase (P...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-013-1413-6
更新日期:2013-08-01 00:00:00
abstract::The effect of the polyamine spermidine on the growth of crown gall tumors was determined using the potato disc bioassay. Addition of lmM spermidine resulted in a 30-50% increase in tumor growth. The spermidine effect was found to be biphasic, with lmM being optimal. Closely related polyamines including spermine, as we...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269212
更新日期:1985-04-01 00:00:00
abstract::We previously reported that Arabidopsis indole-3-acetic acid (IAA)-methyltransferase-1 (IAMT1) catalyzes the conversion of IAA, an essential phytohormone, to methyl-IAA (MeIAA) and that IAMT1 plays an important role in leaf development. Here, we present the possible mechanisms of action of MeIAA in Arabidopsis. We sho...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-007-0458-9
更新日期:2008-03-01 00:00:00
abstract::Somatic hybrids between Solanum tuberosum cv 'Brodick' and S. sanctae-rosae were selected for having elevated chromosome numbers, intermediate morphology and potato cyst nematode resistance (derived from S. sanctae-rosae). DNA was extracted from this material and subjected to inter-SSR PCR directed by oligonucleotide ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050735
更新日期:1999-12-01 00:00:00
abstract::The sequences of the 3' untranslated region (UTR) of the manganese superoxide dismutase (MnSOD) genes in wheat (Triticum aestivum) were found to be quite variable with different predicted thermostabilities. The degradation rates of the 3' UTR variants and the coding region were measured following exposure to endogenou...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0046-9
更新日期:2006-03-01 00:00:00
abstract::Tef [Eragrostis tef (Zucc.) Trotter] is the most important cereal in Ethiopia. In its wild relative E. mexicana, regeneration of six green plants resulted from culture of 121 non-pollinated immature pistils. In the allotetraploid crop species tef, however, only callus and root formation was obtained by this method. By...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0200-z
更新日期:2006-12-01 00:00:00
abstract::A transformed root clone of Datura candida×D. aurea was established following infection with Agrobacterium rhizogenes strain A4. This clone was examined for its growth and hyoscyamine and scopolamine content under various culture conditions. Among the three basal culture media tested, half-strength Gamborg's B5 medium...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050415
更新日期:1998-03-01 00:00:00
abstract::Putrescine, spermidine, and spermine levels during somatic embryogenesis of interior spruce (Picea glauca x Picea engelmannii complex) were quantified On abscisic acid supplemented growth medium putrescine and spermidine levels increased two-fold coinciding with maturation of the early somatic embryos to globular embr...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232981
更新日期:1996-03-01 00:00:00
abstract::In maize, Mutator transposable elements are either active or silenced within the genome. In response to environmental stress, silenced Mutator elements could be reactivated, leading to changes in genome structure and gene function. However, there is no direct experimental evidence linking environmental stress and Muta...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0922-9
更新日期:2010-12-01 00:00:00
abstract::Approximately 10(6) protoplast-derived cell colonies of sainfoin were stressed with streptomycin and two resistant colonies were recovered. Plants regenerated from these colonies could be recallused on streptomycin-containing medium three years after growth in the absence of the antibiotic.Ultrastructural studies show...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269636
更新日期:1986-12-01 00:00:00
abstract::Brassica juncea L. is a Zn/Cd accumulator. To determine the physiological basis of its metal accumulation phenotype, the functional properties and role of the metal efflux transporter BjCET2 were investigated using transgenic technology. Heterologous expression of BjCET2 in the double mutant yeast strain Deltazrc1Delt...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-009-0723-1
更新日期:2009-08-01 00:00:00
abstract:KEY MESSAGE:TaUBA functions as a negative regulator of salt and drought stress response in transgenic Arabidopsis, either the UBA domain or the zinc finger domain is crucial for TaUBA's function. TaUBA (DQ211935), which is a UBA domain-containing protein in wheat, was cloned and functionally characterized. Southern blo...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1739-3
更新日期:2015-05-01 00:00:00
abstract:KEY MESSAGE:Transcript profiling during the early induction phase of oil palm tissue culture and RNAi studies in a model somatic embryogenesis system showed that EgENOD93 expression is essential for somatic embryogenesis. Micropropagation of oil palm through tissue culture is vital for the generation of superior and un...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-020-02571-7
更新日期:2020-11-01 00:00:00
abstract::Cell proliferation has been induced from the cambial zone of a branch of Acacia senegal, kept on the basal Knop and Ball medium in the presence of auxin. Transferred on the more complete nutrient medium of Schenk and Hildebrandt, the colonies gave rise to several cell lines. One of the friable lines, consisting of agg...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00268603
更新日期:1986-10-01 00:00:00
abstract::Protoplasts were isolated from embryogenic callus ofAbies alba L. which originated from immature seeds. The protoplasts were immobilized in alginate beads in order to follow the development of single protoplasts.Surrounding culture medium was modified from Kao and Michayluk (1975). After cell wall regeneration subsequ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00716854
更新日期:1989-02-01 00:00:00
abstract::Callus derived from stem segments of loblolly pine (Pinus taeda L.) was subcultured to media containing 0.5% of various mono-, di-, tri- or polysaccharides. None of the carbohydrate sources tested were superior to sucrose. Growth on twelve of the 20 carbohydrates tested was more than 75% of that obtained for sucrose; ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00276676
更新日期:1987-04-01 00:00:00