Reactivation of a silenced minimal Mutator transposable element system following low-energy nitrogen ion implantation in maize.

Abstract:

:In maize, Mutator transposable elements are either active or silenced within the genome. In response to environmental stress, silenced Mutator elements could be reactivated, leading to changes in genome structure and gene function. However, there is no direct experimental evidence linking environmental stress and Mutator transposon reactivation. Using a maize line that contains a single inactive MuDR and a lone nonautonomous Mutator element, a Mu1 insertion in the recessive reporter allele a1-mum2 in an inactive Mutator background, we directly assessed Mutator reactivation following low-energy nitrogen ion implantation. We observed that N(+) implantation decreased cytosine methylation in MuDR terminal inverted repeats and increased expression of mudrA and mudrB. Both changes were associated with increased transpositional activity of MuDR through reactivation of the inactive minimal Mutator transposable element system. This study provides direct evidence linking environmental stress agents and Mutator transposon mobilization in maize. In addition, the observed changes to DNA methylation suggest a new mechanism for mutations by low-energy ion implantation.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Qian Y,Cheng X,Liu Y,Jiang H,Zhu S,Cheng B

doi

10.1007/s00299-010-0922-9

subject

Has Abstract

pub_date

2010-12-01 00:00:00

pages

1365-76

issue

12

eissn

0721-7714

issn

1432-203X

journal_volume

29

pub_type

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