Abstract：
:Previous work has shown that in rice suspension cells, NaCl at 0.4 M can induce Em gene expression and act synergistically with ABA, possibly by potentiating the ABA response pathway through a rate-limiting intermediate (R.M. Bostock and R.S. Quatrano (1992) Plant Physiol., 98, 1356-1363). Since calcium is an intermediate in ABA regulation of stomatal closure, we tested the effect of calcium changes on ABA-inducible Em gene expression in transiently transformed rice protoplasts. We show that calcium is required for ABA-inducible Em-GUS expression and can act in synergy with ABA. The trivalent ions lanthanum, gadolinium, and aluminum, which are known to interact with calcium- and other signaling pathways, can act at sub-millimolar concentrations to increase GUS reporter gene expression driven by several promoters in transiently transformed rice protoplasts. This effect is not specific for the ABA-inducible Em promoter, but is synergistic with ABA. The lanthanum synergy with ABA does not require calcium. In rice suspension cells, lanthanum alone does not induce Em gene expression, in contrast to transiently transformed protoplasts, yet can act synergistically with ABA to effectively increase the sensitivity to ABA greater than tenfold. Trivalent ions may be a useful tool to study the regulation of gene expression. The possible effects of trivalent ions on ABA signal transduction and gene expression are discussed.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Rock CD,Quatrano RSdoi
10.1007/BF00232374subject
Has Abstractpub_date
1996-01-01 00:00:00pages
371-6issue
5eissn
0721-7714issn
1432-203Xjournal_volume
15pub_type
杂志文章abstract：KEY MESSAGE:A multi-parallel approach gauging the mass spectrometry-based metabolite fingerprinting coupled with bioactivity and pathway evaluations could serve as an efficacious tool for inferring plant taxonomic orders. Thirty-four species from three plant families, namely Cornaceae (7), Fabaceae (9), and Rosaceae (1...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-016-2006-y
更新日期：2016-09-01 00:00:00
abstract：KEY MESSAGE:Tetraploid clementine displays mainly tetrasomic inheritance. Genetic structures of 2n SDR and 2 × gametes from DD clementine are complementary and will guides triploids citrus breeding strategies. Triploid breeding is developed worldwide to create new seedless cultivars. Citrus triploid hybrids can be reco...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-016-1972-4
更新日期：2016-08-01 00:00:00
abstract：:The nucleotide sequence of the cucumber (Cucumis sativus L. cv. Baekmibaekdadagi) chloroplast genome was completed. The circular double-stranded DNA, consisting of 155,527 bp, contained a pair of inverted repeat regions (IRa and IRb) of 25,187 bp each, which were separated by small and large single copy regions of 86,...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0097-y
更新日期：2006-04-01 00:00:00
abstract：:Quantitative real-time polymerase chain reaction (PCR) assays were designed that enabled the zygosity of transgenes in soybean [Glycine max (L.) Merrill] and peanut (Arachis hypogaea L.) to be determined. The two zygosity assays, based on TaqMan technology that uses a fluorogenic probe which hybridizes to a PCR target...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990100326
更新日期：2001-07-01 00:00:00
abstract：:Cryopreservation has been shown to improve the frequency of virus elimination - specifically cucumber mosaic virus and banana streak virus - from banana ( Musa spp.) plants. To understand the mode of action of cryopreservation for the eradication of viral particles, we examined the ultrastructure of meristem tips at e...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-002-0537-x
更新日期：2003-03-01 00:00:00
abstract：:In this paper we describe a simple method to improve the plating efficiency in plant cell cultures.Two-stage plating is used; in the first stage the cells are inoculated at high density in 0.2% agarized culture medium for ten days to facilitate growth; under this condition, each cell produces a single micro-colony tra...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00571305
更新日期：1985-05-01 00:00:00
abstract：:Plating rice anthers on a semisolid induction medium containing 250 or 500 mg/l colchicine for 24 or 48 h-incubations followed by transfer to colchicine-free medium and standard anther culture procedures resulted in overall 1.5- to 2.5- fold increases in doubled haploid green plant productions compared to control anth...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00231961
更新日期：1994-05-01 00:00:00
abstract：:Valencia orange [Citrus sinensis (L.) Osbeck] is the leading commercial citrus species in the world for processed juice products; however, the presence of thermostable pectin methylesterase (TSPME) reduces its juice quality. A long-term strategy of this work is to eliminate or greatly reduce TSPME activity in Valencia...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0952-x
更新日期：2005-10-01 00:00:00
abstract：:Embryo development and germination of Cyclamen persicum have been comparatively characterized for zygotic and somatic embryos with regard to mitotic activity and morphology in order to identify developmental abnormalities in somatic embryogenesis. Zygotic embryo development proved to be highly synchronous with distinc...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0130-9
更新日期：2006-07-01 00:00:00
abstract：:Transgenic plants offer advantages for biomolecule production because plants can be grown on a large scale and the recombinant macromolecules can be easily harvested and extracted. We introduced an Aspergillus phytase gene into canola (Brassica napus) (line 9412 with low erucic acid and low glucosinolates) by Agrobact...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0036-y
更新日期：2006-03-01 00:00:00
abstract：KEY MESSAGE:Transcript profiling during the early induction phase of oil palm tissue culture and RNAi studies in a model somatic embryogenesis system showed that EgENOD93 expression is essential for somatic embryogenesis. Micropropagation of oil palm through tissue culture is vital for the generation of superior and un...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-020-02571-7
更新日期：2020-11-01 00:00:00
abstract：:Histone deacetylases (HDACs) mediate histone deacetylation and act in concert with histone acetyltransferases to regulate dynamic and reversible histone acetylation which modifies chromatin structure and function, affects gene transcription, thus, controlling multiple cellular processes. HDACs are widely distributed i...
journal_title：Plant cell reports
pub_type： 杂志文章,评审
doi：10.1007/s00299-013-1393-6
更新日期：2013-04-01 00:00:00
abstract：:A selectable marker gene (SMG), usually conferring resistance to an antibiotic or herbicide, is generally introduced into the plant cells with the gene(s) for the trait of interest to allow only the cells that have integrated and express the foreign sequences to regenerate into a plant. The availability of several SMG...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-007-0321-z
更新日期：2007-07-01 00:00:00
abstract：:Somatic embroys and subsequent plant regeneration were obtained from isolated leaf epidermis of Gaillardia picta. Abaxial and adaxial epidermal peels (monolayer) from 45 days old aseptic seedlings were isolated and segments measuring 5 mm x 3 mm were cultured on B5 basal medium supplemented with various growth regulat...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232378
更新日期：1992-01-01 00:00:00
abstract：:Developmental biology studies in general benefit from model organisms that are well characterized. Arabidopsis thaliana fulfills this criterion and represents one of the best experimental systems to study developmental processes in higher plants. Light is a crucial factor that drives photosynthesis, but that also regu...
journal_title：Plant cell reports
pub_type： 杂志文章,评审
doi：10.1007/s00299-014-1591-x
更新日期：2014-05-01 00:00:00
abstract：KEY MESSAGE:Two lines of Taxus × media hairy roots harbouring or not the TXS transgene demonstrated diverse gene expression and taxane yield during cultivation in PFD-supported two liquid-phase culture system. Two lines of Taxus × media hairy roots were subjected to single or twice-repeated supplementation with methyl ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-018-2351-0
更新日期：2019-01-01 00:00:00
abstract：: Cells of a suspension culture of Doritaenopsis cv. New Toyohashi were placed in a mixture of 2 M glycerol and 0.4 M sucrose for 15 min at room temperature and then dehydrated with a vitrification solution (PVS2) for 1-3 h on ice and plunged into liquid nitrogen. The highest viability (64% by 2,3,5-triphenyltetrazoliu...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990000255
更新日期：2000-12-01 00:00:00
abstract：:Root vigor is an important trait for the growth of terrestrial plants, especially in water-deficit environments. Although deserts plants are known for their highly developed root architecture, the molecular mechanism responsible for this trait has not been determined. Here we established an efficient protocol for the ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-010-0863-3
更新日期：2010-07-01 00:00:00
abstract：:Using a conventional sexual crossing technique, Nicotiana tabacum x N. acuminata was not produced. After the fertilized ovules were cultured for 20 days in a liquid Nitsch H medium, germination was observed. The roots grew rapidly but leaves did not. However, plantlets were produced in an H medium containing Benzylade...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00268613
更新日期：1986-10-01 00:00:00
abstract：:In leek (Allium ampeloprasum L.) a cyclic system of somatic embryogenesis was developed. Somatic embryos used for cyclic embryogenesis were able to develop the same type of embryogenic callus as zygotic embryos in the primary cycle. For the first time a comparison of the efficiencies of both expiants was made. Ten fam...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233638
更新日期：1995-01-01 00:00:00
abstract：KEY MESSAGE:Overexpression of SoSnRK2.1 improved drought tolerance and growth of tobacco plants. Sucrose non-fermenting1-related protein kinase 2 (SnRK2) is a key enzyme in regulating ABA signal transduction in plants, and it plays a significant role in response to multiple abiotic stresses. In this research, SoSnRK2.1...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-016-2004-0
更新日期：2016-09-01 00:00:00
abstract：:Protoplasts isolated from cultured rice cells of an A-58 cytoplasmic male sterile line (A-58 MS)(Oryza sativa L.) were used to investigate the regeneration of rice plants. A cultured cell line (T3) of A-58 MS with a high growth rate and dense cytoplasm was selected. About 10% of the protoplasts prepared from this esta...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269240
更新日期：1986-04-01 00:00:00
abstract：:The voltage-dependent anion channels (VDACs), mitochondrial outer membrane components, are present in organisms from fungi to animals and plants. They are thought to function in the regulation of metabolite transport between mitochondria and the cytoplasm. Sufficient knowledge on plant VDACs has been accumulated, so t...
journal_title：Plant cell reports
pub_type： 杂志文章,评审
doi：10.1007/s00299-009-0741-z
更新日期：2009-09-01 00:00:00
abstract：KEY MESSAGE:Arabidopsis GI negatively regulates chloroplast biogenesis and resistance to the herbicide butafenacil by enhanced activity and transcriptional levels of antioxidant enzymes Chloroplast biogenesis is blocked by retrograde signaling triggered by diverse internal and external cues, including sugar, reactive o...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-019-02409-x
更新日期：2019-07-01 00:00:00
abstract：KEY MESSAGE:A R2R3-MYB transcription factor EsAN2 was isolated from Epimedium sagittatum and functionally characterized to regulate the anthocyanin biosynthetic pathway. Epimedium plants are used widely both as traditional Chinese medicinal herbs and ornamental perennials. Anthocyanins, acting as major contributors to ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-015-1929-z
更新日期：2016-04-01 00:00:00
abstract：KEY MESSAGE:A chalcone synthase gene ( PaCHS ) was isolated and functionally characterized from liverwort. The ectopic expression of PaCHS in Marchantia paleacea callus raised the flavonoids content. Chalcone synthase (CHS; EC 2.3.1.74) is pivotal for the biosynthesis of flavonoid and anthocyanin pigments in plants. It...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1702-8
更新日期：2015-02-01 00:00:00
abstract：:Somatic hybrids between Solanum tuberosum cv 'Brodick' and S. sanctae-rosae were selected for having elevated chromosome numbers, intermediate morphology and potato cyst nematode resistance (derived from S. sanctae-rosae). DNA was extracted from this material and subjected to inter-SSR PCR directed by oligonucleotide ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050735
更新日期：1999-12-01 00:00:00
abstract：:The conserved domains of reverse transcriptase (RT) genes of Ty1-copia and Ty3-gypsy groups of long terminal repeat (LTR) retrotransposons were amplified from mungbean (Vigna radiata) genome using degenerate primers, cloned and sequenced. Among these 34% and 65% of respective clones of copia and gypsy RT sequences pos...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0008-2
更新日期：2006-03-01 00:00:00
abstract：:Hypocotyl explants of Albizzia julibrissin were placed on Gamborg's B5 medium supplemented with various levels of paclobutrazol, uniconazole, prohexadione calcium, or GA3. Callus formation was evident within one week after placement of the explants on the culture media. Green nodule-like structures protruded from the ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00235303
更新日期：1993-12-01 00:00:00
abstract：:Plant regeneration in Arachis pintoi was obtained via two developmental pathways: organogenesis and somatic embryogenesis. Organogenic callus cultures were initiated from pieces of leaf on MS medium supplemented with NAA or 2,4-D in combination with BA, KIN or 2iP. The most suitable combination for plant regeneration ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990000198
更新日期：2000-09-01 00:00:00
