Production of Nicotiana tabacum x Nicotiana acuminata hybrid by ovule culture.

Abstract:

:Using a conventional sexual crossing technique, Nicotiana tabacum x N. acuminata was not produced. After the fertilized ovules were cultured for 20 days in a liquid Nitsch H medium, germination was observed. The roots grew rapidly but leaves did not. However, plantlets were produced in an H medium containing Benzyladenine or Kinetin (0.01-0.1 mg/l). The plantlets grew and flowered in a greenhouse. The chromosome number of the hybrid was 36 and its morphological characteristics were intermediate between those of parental species.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Iwai S,Kishi C,Nakata K,Kawashima N

doi

10.1007/BF00268613

subject

Has Abstract

pub_date

1986-10-01 00:00:00

pages

403-4

issue

5

eissn

0721-7714

issn

1432-203X

journal_volume

5

pub_type

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