Regeneration of plants from callus tissue of the pasture legume Centrosema brasilianum.

Abstract:

:Plants were regenerated from leaf explants of Centrosema brasilianum cultured in vitro. Callus and buds were produced on Murashige and Skoog medium (MS), 0.8% agar, 0.1 mg/l NAA and 1 mg/l BAP. Regeneration of multiple shoots was achieved by transferring callus onto fresh medium containing 0.01 and 1 mg/l of NAA and BAP, respectively. Shoots formed roots upon transfer to MS with 0.01 mg/l NAA. Plantlets were succesfully transferred to soil. Leaf-derived calli of Centrosema arenarium, C. macrocarpum, C. pascuorum, C. pubescens, and C. virginianum did not produce shoots when cultured in vitro.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Angeloni PN,Rey HY,Mroginski LA

doi

10.1007/BF00236268

subject

Has Abstract

pub_date

1992-09-01 00:00:00

pages

519-21

issue

10

eissn

0721-7714

issn

1432-203X

journal_volume

11

pub_type

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