Abstract:
:An Arabidopsis mutant line T90, exhibiting a stem-specific and wound-responsive GUS expression was identified from a population of Arabidopsis thaliana tagged with a promoterless β-glucuronidase (GUS) in the T-DNA. Sequence flanking the insertion from the right border was amplified by TAIL PCR and cloned. The insertion was located in the third chromosome, 57 bp upstream of the ATG start codon in 5' untranslated region (UTR) of the fatty acyl-CoA reductase 6 (FAR6) gene. RT-PCR analysis of the FAR6 gene revealed that the gene is expressed predominantly in stem tissue. Semi-quantitative RT-PCR showed that the expression is also induced by wounding in the epidermal layer of mature stem internodes. The transcription initiation site (TSS) was identified by 5' RACE PCR. Different 5' deletion fragments of the promoter sequences were developed and linked to the GUS reporter gene as transcriptional fusions and the expression patterns of GUS were histochemically analyzed in transgenic Arabidopsis plants. Sequences from -510 bp upstream to the transcriptional start site were sufficient to exhibit wound-inducible GUS expression in the stems. The addition of further upstream sequences (-510 to -958, -1,400 or -1,456) enhanced and extended the wound-inducible GUS expression throughout the mature stem.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Gupta NC,Jain PK,Bhat SR,Srinivasan Rdoi
10.1007/s00299-011-1205-9subject
Has Abstractpub_date
2012-05-01 00:00:00pages
839-50issue
5eissn
0721-7714issn
1432-203Xjournal_volume
31pub_type
杂志文章abstract::It has been reported that long term cell cultures from maize endosperms are not completely de-differentiated, maintaining some tissue-specific synthesis. We analyzed the expression of zein (the major storage protein of maize seeds) in cultures derived from wildtype and opaque-2 maize endosperms. In wildtype cultures, ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272049
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journal_title:Plant cell reports
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journal_title:Plant cell reports
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272972
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journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s002990100346
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050520
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-002-0550-0
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233291
更新日期:1994-11-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231971
更新日期:1994-05-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01088288
更新日期:1997-02-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233367
更新日期:1992-07-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272634
更新日期:1982-12-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-016-2017-8
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233152
更新日期:1996-08-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232932
更新日期:1994-08-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00274124
更新日期:1989-05-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1080-4
更新日期:2011-09-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232412
更新日期:1992-12-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
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journal_title:Plant cell reports
pub_type: 杂志文章
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journal_title:Plant cell reports
pub_type: 杂志文章,评审
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更新日期:2019-09-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
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更新日期:2009-11-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
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更新日期:1995-01-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-016-2065-0
更新日期:2017-01-01 00:00:00
abstract:KEY MESSAGE:Transgenic Arabidopsis and lettuce plants overexpressing AtHSP17.8 showed ABA-hypersensitive but abiotic stress-resistant phenotypes. ABA treatment caused a dramatic induction of early ABA-responsive genes in AtHSP17.8 -overexpressing transgenic lettuce. Plant small heat shock proteins function as chaperone...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-013-1506-2
更新日期:2013-12-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232455
更新日期:1996-04-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1016-z
更新日期:2011-05-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232364
更新日期:1996-01-01 00:00:00
abstract::Ginger (Zingiber officinale Roscoe) cultivars are susceptible to soft rot disease caused by Pythium aphanidermatum. We analyzed changes in transcript levels of 41 genes in the highly susceptible ginger cultivar varada, a less susceptible wild accession (wild ginger), and a Pythium aphanidermatum-resistant relative, Z....
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0594-x
更新日期:2008-11-01 00:00:00