Abstract:
:Extracts of cranberry, rich in flavonols and proanthocyanins, inactivated β-glucuronidase (GUS) in assays using either purified bacterial GUS or preparations of transgenic tobacco (Nicotiana tabacum L.) or transgenic cranberry (Vaccinium macrocarpon Ait.) expressing the gusA gene. Histochemical GUS assays produced random and generally unpredictable staining. The addition of polyvinylpolypyrrolidone (PVPP) during the preparation of transgenic cranberry leaf extracts increased the detectable GUS activity in fluorogenic assays more than 200-fold. Detectable GUS activity varied among transclones and also within a transclone depending on the developmental and physiological state of the tissue, as well as the growth environment. Use of PVPP altered the relative ranking of plants based on their total transgenic enzyme activity and resulted in different conclusions as to the effects of genotype or growth environment on transgene expression.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Serres R,McCown B,Zeldin Edoi
10.1007/s002990050294subject
Has Abstractpub_date
1997-06-01 00:00:00pages
641-646issue
9eissn
0721-7714issn
1432-203Xpii
10.1007/s002990050294journal_volume
16pub_type
杂志文章abstract::A DNA fragment encoding a 12-amino acid (aa) HIV-1 Tat transduction peptide fused to a 90-aa murine rotavirus NSP4 enterotoxin protein (Tat-NSP4(90)) was transferred to Solanum tuberosum by Agrobacterium tumefaciens-mediated transformation. The fusion gene was detected in the genomic DNA of transformed plant leaf tiss...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0697-3
更新日期:2004-01-01 00:00:00
abstract::Rhodamine-6G(R6G), a lipophilic dye which degrades mammalian mitochondria, was shown to arrest the division of Nicotiana protoplasts. When albino recipient-protoplasts were treated with R6G and fused with X-irradiated (green) donor- protoplasts, only green cybrid plants were obtained. The mtDNA of the cybrids was anal...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269126
更新日期:1986-06-01 00:00:00
abstract::Anthocyanin is the primary pigment contributing to red, violet, and blue flower color formation. The solubility of anthocyanins is enhanced by UDP glucose: flavonoid 3-O-glucosyltransferase (UFGT) through transfer of the glucosyl moiety from UDP-glucose to 3-hydroxyl group to produce the first stable pigments. To asse...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1006-1
更新日期:2011-06-01 00:00:00
abstract::Jasmonates enhance the expression of various genes involved in terpenoid indole alkaloid (TIA) biosynthesis in Catharanthus roseus. We applied precursor feeding to our C. roseus suspensions to determine how methyl jasmonate (MJ) alters the precursor availability for TIA biosynthesis. C. roseus suspensions were induced...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0109-y
更新日期:2006-06-01 00:00:00
abstract::A simple method using molecular hybridization was devised to quantitatively measure chloroplast DNA synthesis in vivo. Total cellular DNA isolated from Nicotiana tabacum suspension cells, labeled with (3)H-thymidine, was hybridized to nitrocellulose membrane-bound cloned chloroplast DNA (ct DNA) fragments. Colorless, ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269202
更新日期:1985-04-01 00:00:00
abstract:: Mature ovules of Dianthus (Caryophyllaceae) were histologically observed by clearing and serial sectioning to characterize the cells of the embryo sac. The results show that the mature embryo sac was located deep inside the hemitropous ovule due to thick nucellar tissue at the micropylar region. For the isolation of ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050753
更新日期:2000-04-01 00:00:00
abstract::Shoot buds of ginger were successfully encapsulated in 4% sodium alginate gel. Encapsulated buds were germinated in vitro to form roots and shoots. In vitro germination (emergence of sprouts) of encapsulated buds ranged from 16.7% to 81.8% on different media after 5 weeks of incubation. Normal plantlets with an averag...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233325
更新日期:1994-02-01 00:00:00
abstract::Randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were used to analyse the genetic diversity of Portuguese Prunus dulcis cultivars and their relationship to important foreign cultivars. Of the primers tested, 6 (out of 60) RAPD and 5 (out of 18) ISSR primers were selected for th...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0659-9
更新日期:2003-08-01 00:00:00
abstract:KEY MESSAGE:Induced mutations in the waxy locus in rice endosperm did not abolish GBSS activity completely. Compensatory mechanisms in endosperm and leaves caused a major reprogramming of the starch biosynthetic machinery. The mutation of genes in the starch biosynthesis pathway has a profound effect on starch quality ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-019-02388-z
更新日期:2019-03-01 00:00:00
abstract::The gene coding for the green fluorescent protein (GFP) from jellyfish was introduced into conifer tissues by microprojectile bombardment and its transient expression was detected. Two versions of the GFP gene, wild-type GFP and modified GFP with a cryptic intron removed, were directly compared for their expression in...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01088278
更新日期:1997-02-01 00:00:00
abstract::Hairy root cultures of Lithospermum canescens were established using three strains of Agrobacterium rhizogenes: ATCC 15834, LBA 9402 and NCIB 8196. Eight lines resulting from infection with A. rhizogenes ATCC 15834 demonstrated sufficient biomass increase and were submitted to further investigations. The contents of a...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0161-2
更新日期:2006-10-01 00:00:00
abstract::The direct induction of adventitious shoots from leaf explants obtained from adult plants of Zhanhua winter jujube, an elite variety of Zizyphus jujuba Mill., is reported. The percentage of leaf explants producing shoots and the average number of shoots per explant were significantly improved when 10-day-old leaves we...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0920-5
更新日期:2005-03-01 00:00:00
abstract:KEY MESSAGE:Drastic changes in soil water content can activate the short-term high expression of key enzyme-encoding genes involved in secondary metabolite synthesis thereby increasing the content of secondary metabolites. Bupleurum chinense DC. is a traditional medicinal herb that is famous for its abundant saikosapon...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-019-02436-8
更新日期:2019-09-01 00:00:00
abstract::The effect ofin planta treatments with auxin inhibitors such as 2,3,5 triiodobenzoic acid (TIBA) on regeneration of plantsin vitro is not known. Here, we show the beneficial effect of preconditioning sugarbeet plants in the greenhouse with TIBA (3 mg/1) for efficientin vitro plant regeneration via a callus phase from ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01890855
更新日期:1996-12-01 00:00:00
abstract::The expression and functionality of a resveratrol synthase (RS) gene from peanut (Arachis hypogaea) was studied using an Agrobacterium tumefaciens-mediated transient expression system in Nicotiana benthamiana leaves. Functional analysis of RS was demonstrated by tracking its expression during 96 h. To measure the tran...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0664-0
更新日期:2009-04-01 00:00:00
abstract::This article describes conditions to optimize the yield of viable protoplasts from callus tissue of Asparagus densiflorus cv. Sprengeri and their subsequent regeneration into plantlets. Callus tissue was initiated by culturing spear sections (5-7 mm) on Murashige and Skoog (MS) medium supplemented with 0.8% (wt/vol) B...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050364
更新日期:1997-12-01 00:00:00
abstract::RNA interference (RNAi) is a sequence-specific, posttranscriptional gene silencing (PTGS) process in plants that is mediated by dsRNA homologous to the silenced gene(s). In this study, we report an efficient method to produce dsRNA using a bacterial expression system. Two fragments of the Sugarcane Mosaic Virus (SCMV)...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0911-z
更新日期:2010-11-01 00:00:00
abstract::A restricted part of the undeveloped stem of the garlic clove, called the "stem disc", which is just under the basement of the immature foliage leaves, proved to be a very potent explant for the micropropagation of garlic. Twenty to thirty tissue-cultured shoots consistently were differentiated from a single clove dur...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050481
更新日期:1998-07-01 00:00:00
abstract:KEY MESSAGE:The Arabidopsis SAUR36, renamed RAG1, integrates auxin and gibberellin signals to regulate apical hook maintenance in etiolated seedlings, hypocotyl elongation in the light and fertility. Phytohormone signalling intermediates integrate responses to developmental cues and the variety of environmental inputs ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-013-1406-5
更新日期:2013-06-01 00:00:00
abstract::A genetic transformation protocol for green ash (Fraxinus pennsylvanica) hypocotyl explants was developed. Green ash hypocotyls were transformed using Agrobacterium tumefaciens strain EHA105 harboring binary vector pq35GR containing the neomycin phosphotransferase (nptII) and beta-glucuronidase (GUS) fusion gene, and ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-009-0697-z
更新日期:2009-06-01 00:00:00
abstract::Growth inhibition towards Rhizopus nigricans, Fusarium oxysporum f. sp. radicis-lycopersici, Verticillium albo-atrum and Pythium ultimum was observed in vitro using a purified chitosanase from an actinomycete, Streptomyces sp, strain N174. The corresponding gene, with its own signal peptide, was inserted into pBI121.7...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00193724
更新日期:1995-12-01 00:00:00
abstract::Most infections of plant roots are initiated in the region of elongation; the mechanism for this tissue-specific localization pattern is unknown. In alfalfa expressing PsUGT1 antisense mRNA under the control of the cauliflower mosaic virus (CaMV) 35S promoter, the cell cycle in roots is completed in 48 h instead of 24...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-004-0787-x
更新日期:2004-07-01 00:00:00
abstract:KEY MESSAGE:Marker-free transgenic plants can be generated with high efficiency by using the Cre/ lox P self-excision system controlled by the pollen- and embryo-specific Arabidopsis DLL promoter. In this work, we aimed to study the feasibility of using the pollen- and embryo-specific DLL promoter of the At4g16160 gene...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1726-0
更新日期:2015-03-01 00:00:00
abstract::A novel member of the WRKY gene family, designated TcWRKY53, was isolated from a cadmium (Cd)-treated Thlaspi caerulescens cDNA library by differential screening. WRKY proteins specifically bind to W-boxes, which are found in the promoters of many genes involved in defense and response to environmental stress. TcWRKY5...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-007-0499-0
更新日期:2008-04-01 00:00:00
abstract::Responses induced by Cd exposure were assessed in tobacco seedlings (Nicotiana tabacum L.) using macro and molecular indices. The 100 microM of Cd exposure reduced the total dry weight and chlorophyll index of the seedlings as much as the genuine Fe-deficiency. Concentration of Fe in the shoots decreased, whereas that...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0092-3
更新日期:2006-04-01 00:00:00
abstract::An improved protocol forAgrobacterium-mediated transformation of the tomato cultivar Moneymaker was developed by examining the effects of six different factors on the efficiency of transformation. Explant size, explant orientation, gelling agent and plate sealant were found to affect transformation efficiency. Two oth...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01890875
更新日期:1996-12-01 00:00:00
abstract:KEY MESSAGE:This work provides a detailed histological analysis of the development of Jatropha curcas seeds, together with an assessment of the role of programmed cell death in this process. Seeds of Jatropha curcas are a potential source of raw material for the production of biodiesel, but very little is known about h...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-017-2184-2
更新日期:2017-11-01 00:00:00
abstract::Morphologically normal green plants have reproducibly been regenerated from protoplasts of an Australian wheat (Triticum aestivum cv. Hartog). The protoplasts were isolated from fine embryogenic suspension cultures which were initiated from embryogenic callus. Protoplasts were incubated in a modified liquid MS medium ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231832
更新日期:1992-02-01 00:00:00
abstract::A cDNA clone encoding a putative RTE1-like protein (Rh-RTH1) was obtained from total RNA isolated from senescing rose (Rosa hybrida cv. Tineke) petals using RT-PCR and RACE techniques. The cDNA (1,061 bp) contained an open reading frame of 684 bp corresponding to 227 amino acids. The amino acid sequence had 60.0, 49.6...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0875-z
更新日期:2010-08-01 00:00:00
abstract:KEY MESSAGE:A R2R3-MYB transcription factor EsAN2 was isolated from Epimedium sagittatum and functionally characterized to regulate the anthocyanin biosynthetic pathway. Epimedium plants are used widely both as traditional Chinese medicinal herbs and ornamental perennials. Anthocyanins, acting as major contributors to ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1929-z
更新日期:2016-04-01 00:00:00