Detectable ,-glucuronidase activity in transgenic cranberry is affected by endogenous inhibitors and plant development.

Abstract:

:Extracts of cranberry, rich in flavonols and proanthocyanins, inactivated β-glucuronidase (GUS) in assays using either purified bacterial GUS or preparations of transgenic tobacco (Nicotiana tabacum L.) or transgenic cranberry (Vaccinium macrocarpon Ait.) expressing the gusA gene. Histochemical GUS assays produced random and generally unpredictable staining. The addition of polyvinylpolypyrrolidone (PVPP) during the preparation of transgenic cranberry leaf extracts increased the detectable GUS activity in fluorogenic assays more than 200-fold. Detectable GUS activity varied among transclones and also within a transclone depending on the developmental and physiological state of the tissue, as well as the growth environment. Use of PVPP altered the relative ranking of plants based on their total transgenic enzyme activity and resulted in different conclusions as to the effects of genotype or growth environment on transgene expression.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Serres R,McCown B,Zeldin E

doi

10.1007/s002990050294

subject

Has Abstract

pub_date

1997-06-01 00:00:00

pages

641-646

issue

9

eissn

0721-7714

issn

1432-203X

pii

10.1007/s002990050294

journal_volume

16

pub_type

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