Downregulation of putative UDP-glucose: flavonoid 3-O-glucosyltransferase gene alters flower coloring in Phalaenopsis.

Abstract:

:Anthocyanin is the primary pigment contributing to red, violet, and blue flower color formation. The solubility of anthocyanins is enhanced by UDP glucose: flavonoid 3-O-glucosyltransferase (UFGT) through transfer of the glucosyl moiety from UDP-glucose to 3-hydroxyl group to produce the first stable pigments. To assess the possibility that UFGT is involved in the flower color formation in Phalaenopsis, the transcriptional activities of PeUFGT3, and other flower color-related genes in developing red or white flower buds were examined using RT-PCR analysis. In contrast with chalcone synthase, chalcone isomerase, and anthocyanidin synthase genes, PeUFGT3 transcriptional activity was higher expressed in the red color of Phalaenopsis cultivars. In the red labellum of Phalaenopsis 'Luchia Lady', PeUFGT3 also showed higher expression levels than that in the white perianth. PeUFGT3 was predominantly expressed in the red region of flower among various Phalaenopsis cultivars. To investigate the role of PeUFGT3 in red flower color formation, PeUFGT3 was specifically knocked down using RNA interference technology via virus inducing gene silencing in Phalaenopsis. The PeUFGT3-suppressed Phalaenopsis exhibited various levels of flower color fading that was well correlated with the extent of reduced level of PeUFGT3 transcriptional activity. Furthermore, there was a significant decrease in anthocyanin content in the PeUFGT3-suppressed Phalaenopsis flowers. The decrease of anthocyanin content due to PeUFGT3 gene silencing possibly caused the faded flower color in PeUFGT3-suppressed Phalaenopsis. Consequently, these results suggested that the glycosylation-related gene PeUFGT3 plays a critical role in red color formation in Phalaenopsis.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Chen WH,Hsu CY,Cheng HY,Chang H,Chen HH,Ger MJ

doi

10.1007/s00299-011-1006-1

subject

Has Abstract

pub_date

2011-06-01 00:00:00

pages

1007-17

issue

6

eissn

0721-7714

issn

1432-203X

journal_volume

30

pub_type

杂志文章
  • Small heat shock protein LimHSP16.45 protects pollen mother cells and tapetal cells against extreme temperatures during late zygotene to pachytene stages of meiotic prophase I in David Lily.

    abstract::Plant meiotic prophase I is a complicated process involving the late zygotene and pachytene stages, both crucial for completing synapsis and recombination. Using David Lily (Lilium davidii var. Willmottiae) as our research material, we performed suppression subtractive hybridization to construct EST library of anthers...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-011-1106-y

    authors: Mu C,Wang S,Zhang S,Pan J,Chen N,Li X,Wang Z,Liu H

    更新日期:2011-10-01 00:00:00

  • Establishment of a transgenic hairy root system in wild and domesticated watermelon (Citrullus lanatus) for studying root vigor under drought.

    abstract::Root vigor is an important trait for the growth of terrestrial plants, especially in water-deficit environments. Although deserts plants are known for their highly developed root architecture, the molecular mechanism responsible for this trait has not been determined. Here we established an efficient protocol for the ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-010-0863-3

    authors: Kajikawa M,Morikawa K,Abe Y,Yokota A,Akashi K

    更新日期:2010-07-01 00:00:00

  • Somatic embryogenesis and in vitro flowering of 3 species of bamboo.

    abstract::Plant regeneration via somatic embryogenesis was achieved in callus cultures derived from nodal explants of in vitro grown seedlings and excised mature zygotic embryos of three bamboo species on Murashige and Skoog's (MS) basal medium supplemented with 0.5 mg/l kinetin (Kn), 2.0 mg/l 2,4-dichlorophenoxyacetic acid (2,...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00231624

    authors: Rout GR,Das P

    更新日期:1994-09-01 00:00:00

  • Cryopreservation of Doritaenopsis suspension culture by vitrification.

    abstract:: Cells of a suspension culture of Doritaenopsis cv. New Toyohashi were placed in a mixture of 2 M glycerol and 0.4 M sucrose for 15 min at room temperature and then dehydrated with a vitrification solution (PVS2) for 1-3 h on ice and plunged into liquid nitrogen. The highest viability (64% by 2,3,5-triphenyltetrazoliu...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s002990000255

    authors: Tsukazaki H,Mii M,Tokuhara K,Ishikawa K

    更新日期:2000-12-01 00:00:00

  • Isolation and culture of protoplasts of pigeonpea (Cajanus cajan L.).

    abstract::High yields of protoplasts were obtained from leaves of aseptically grown plants and calli originated from different explants, in several cultivars of Cajanus cajan L. The protoplasts divided to form cell clusters in modified KM 8p medium and developed to protocolonies after dilution with liquid Caboche's medium withi...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232691

    authors: Sarangi BK,Kuchuk N,Gleba YY

    更新日期:1992-08-01 00:00:00

  • Regeneration of protoplast-derived green plants of Kentucky blue grass (Poa pratensis L.).

    abstract::Green plants were repeatedly regenerated from suspension-derived protoplasts of Kentucky blue grass (Poa pratensis L.) cv. "Geronimo". One suspension was capable of donating competent protoplasts during long-term culture i. e. 10-16 months after its establishment. The plating efficiency of the protoplasts from three d...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00233055

    authors: Nielsen KA,Larsen E,Knudsen E

    更新日期:1993-08-01 00:00:00

  • Increased doubled haploid plant regeneration from rice (Oryza sativa L.) anthers cultured on colchicine-supplemented media.

    abstract::Plating rice anthers on a semisolid induction medium containing 250 or 500 mg/l colchicine for 24 or 48 h-incubations followed by transfer to colchicine-free medium and standard anther culture procedures resulted in overall 1.5- to 2.5- fold increases in doubled haploid green plant productions compared to control anth...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00231961

    authors: Alemanno L,Guiderdoni E

    更新日期:1994-05-01 00:00:00

  • Phenylalanine ammonia-lyase, phenolic acids and ethylene in alfalfa (Medicago sativa L.) cell cultures in relation to their embryogenic ability.

    abstract::Phenylalanine ammonia-lyase (PAL) activity, contents of phenolic acids and ethylene production during the lag-phase, and contents of phenolic acids at the late exponential phase, showed significant differences in embryogenic (EC) and non-embryogenic (NEC) suspension cultures of Medicago sativa L. Maximum PAL activity ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232569

    authors: Cvikrová M,Meravý L,Macháčková I,Eder J

    更新日期:1991-08-01 00:00:00

  • Transient gene expression after electroporation of protoplasts derived from embryogenic maize callus.

    abstract::Protoplasts isolated from embryogenic callus cultures derived from immature embryos ofZea mays L. are suitable for analysis of transient gene expression using electroporation-mediated DNA transfer. Expression of introduced genes is comparable to the levels obtained with protoplasts from Black Mexican Sweet suspension ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00716827

    authors: Planckaert F,Walbot V

    更新日期:1989-03-01 00:00:00

  • A method for the rapid production of fine plant cell suspension cultures.

    abstract::A method has been devised which allows the rapid production of fine suspension cultures of small aggregate size from suspension cultures of large average aggregate size, such as those of Capsicum frutescens. The method, which uses a Waring blender for aseptic homogenisation of cultures, has also been shown to be effec...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269538

    authors: Williams PD,Wilkinson AK,Lewis JA,Black GM,Mavituna F

    更新日期:1988-10-01 00:00:00

  • Myb14, a direct activator of STS, is associated with resveratrol content variation in berry skin in two grape cultivars.

    abstract:KEY MESSAGE:High and low resveratrol (Res) contents in two cultivars are correlated with the expression abundance of Myb14 , which could directly activate transcriptional expression of stilbene synthase gene ( STS ). Resveratrol (3,5,4'-trihydroxystilbene) is one of the natural polyphenols produced by secondary metabol...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-014-1642-3

    authors: Fang L,Hou Y,Wang L,Xin H,Wang N,Li S

    更新日期:2014-10-01 00:00:00

  • In vitro adventitious shoot production inPaulownia.

    abstract::In vitro adventitious shoot formation was examined in petioles and laminae ofPaulownia elongata, P. fortunei, andP. 'Henan 1'. Fully expanded, dark green, thick, older leaves were healthier and exhibited greater callus and shoot production than did young leaves. The ten clones used varied greatly in adventitious shoot...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF01088288

    authors: Bergmann BA,Moon HK

    更新日期:1997-02-01 00:00:00

  • Progress of tissue culture and genetic transformation research in pigeon pea [Cajanus cajan (L.) Millsp.].

    abstract::Pigeon pea [Cajanus cajan (L.) Millsp.] (Family: Fabaceae) is an important legume crop cultivated across 50 countries in Asia, Africa, and the Americas; and ranks fifth in area among pulses after soybean, common bean, peanut, and chickpea. It is consumed as a major source of protein (21%) to the human population in ma...

    journal_title:Plant cell reports

    pub_type: 杂志文章,评审

    doi:10.1007/s00299-010-0899-4

    authors: Krishna G,Reddy PS,Ramteke PW,Bhattacharya PS

    更新日期:2010-10-01 00:00:00

  • Stable endosperm cell suspension cultures from wild-type and opaque-2 maize.

    abstract::Stable cell suspension cultures have been established from immature endosperms of A69Y wild-type and opaque-2 maize (Zea mays L.). Cultured cells are capable of storage protein (zein) synthesis and accumulation throughout the growth period. Electrophoretic patterns of zeins show, for opaque-2 cells, the preferential i...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232330

    authors: Manzocchi LA

    更新日期:1991-02-01 00:00:00

  • Plant regeneration from protoplasts of wheat (Triticum aestivum cv. Hartog).

    abstract::Morphologically normal green plants have reproducibly been regenerated from protoplasts of an Australian wheat (Triticum aestivum cv. Hartog). The protoplasts were isolated from fine embryogenic suspension cultures which were initiated from embryogenic callus. Protoplasts were incubated in a modified liquid MS medium ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00231832

    authors: He DG,Yang YM,Scott KJ

    更新日期:1992-02-01 00:00:00

  • The possible action mechanisms of indole-3-acetic acid methyl ester in Arabidopsis.

    abstract::We previously reported that Arabidopsis indole-3-acetic acid (IAA)-methyltransferase-1 (IAMT1) catalyzes the conversion of IAA, an essential phytohormone, to methyl-IAA (MeIAA) and that IAMT1 plays an important role in leaf development. Here, we present the possible mechanisms of action of MeIAA in Arabidopsis. We sho...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-007-0458-9

    authors: Li L,Hou X,Tsuge T,Ding M,Aoyama T,Oka A,Gu H,Zhao Y,Qu LJ

    更新日期:2008-03-01 00:00:00

  • Expression analysis of defense-related genes in Zingiber (Zingiberaceae) species with different levels of compatibility to the soft rot pathogen Pythium aphanidermatum.

    abstract::Ginger (Zingiber officinale Roscoe) cultivars are susceptible to soft rot disease caused by Pythium aphanidermatum. We analyzed changes in transcript levels of 41 genes in the highly susceptible ginger cultivar varada, a less susceptible wild accession (wild ginger), and a Pythium aphanidermatum-resistant relative, Z....

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-008-0594-x

    authors: Kavitha PG,Thomas G

    更新日期:2008-11-01 00:00:00

  • Multiple shoot induction by benzyladenine and complete plant regeneration from seed explants of chickpea (Cicer arietinum L.).

    abstract::The efficacy of benzyladenine (BA) to induce multiple shoots from seed explants of chickpea (Cicer arietinum L.) was assessed. Shoot differentiation was influenced by the type of seed explant, genotype and concentration of BA. Orientation of the explant also strongly influenced the shoot regeneration response. The opt...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF01142325

    authors: Polisetty R,Paul V,Deveshwar JJ,Khetarpal S,Suresh K,Chandra R

    更新日期:1997-05-01 00:00:00

  • Phytochelatin accumulation and stress tolerance in tomato cells exposed to cadmium.

    abstract::Cell suspension cultures of tomato (Lycopersicon esculentum) adapted to growing continuously in the presence of 0.1 mM CdCl2 and accumulated phytochelatins (PCs, poly(γ-Glu-Cys)n-Gly). The highest level of PCs was measured 4 days after inoculation and coincided with the peak of cellular cadmium concentration. At this ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232275

    authors: Gupta SC,Goldsbrough PB

    更新日期:1990-12-01 00:00:00

  • Ploidy variation among herbicide-resistant bermudagrass plants of cv. TifEagle transformed with the bar gene.

    abstract::A protocol was developed for biolistic transformation of hybrid bermudagrass cv. TifEagle using the bar gene. TifEagle is an ultradwarf used exclusively on golf greens. Herbicide resistance should serve as a useful management tool, especially if methyl-bromide is unavailable for fumigation prior to plant establishment...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-003-0736-0

    authors: Goldman JJ,Hanna WW,Fleming GH,Ozias-Akins P

    更新日期:2004-03-01 00:00:00

  • An examination of factors affecting the efficiency ofAgrobacterium-mediated transformation of tomato.

    abstract::An improved protocol forAgrobacterium-mediated transformation of the tomato cultivar Moneymaker was developed by examining the effects of six different factors on the efficiency of transformation. Explant size, explant orientation, gelling agent and plate sealant were found to affect transformation efficiency. Two oth...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF01890875

    authors: Frary A,Earle ED

    更新日期:1996-12-01 00:00:00

  • Reverse transcriptase domain sequences from Mungbean (Vigna radiata) LTR retrotransposons: sequence characterization and phylogenetic analysis.

    abstract::The conserved domains of reverse transcriptase (RT) genes of Ty1-copia and Ty3-gypsy groups of long terminal repeat (LTR) retrotransposons were amplified from mungbean (Vigna radiata) genome using degenerate primers, cloned and sequenced. Among these 34% and 65% of respective clones of copia and gypsy RT sequences pos...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-005-0008-2

    authors: Dixit A,Ma KH,Yu JW,Cho EG,Park YJ

    更新日期:2006-03-01 00:00:00

  • Cadmium inducible Fe deficiency responses observed from macro and molecular views in tobacco plants.

    abstract::Responses induced by Cd exposure were assessed in tobacco seedlings (Nicotiana tabacum L.) using macro and molecular indices. The 100 microM of Cd exposure reduced the total dry weight and chlorophyll index of the seedlings as much as the genuine Fe-deficiency. Concentration of Fe in the shoots decreased, whereas that...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-005-0092-3

    authors: Yoshihara T,Hodoshima H,Miyano Y,Shoji K,Shimada H,Goto F

    更新日期:2006-04-01 00:00:00

  • Differences in pectic polysaccharides between carrot embryogenic and non-embryogenic calli.

    abstract::Cell walls and media were obtained from three kinds of carrot cell culture, namely, embryogenic callus (EC), non-embryogenic callus (NC) and somatic embryos (SE), and analyzed for their sugar content and sugar composition by electrophoresis and gas chromatography. EC formed large cell clusters while NC formed small cl...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232028

    authors: Kikuchi A,Satoh S,Nakamura N,Fujii T

    更新日期:1995-02-01 00:00:00

  • Efficient callus formation and plant regeneration from leaves of oats (Avena sativa L.).

    abstract::An efficient plant regeneration system from leaf-derived callus in 6 genotypes of Avena sativa L. has been established. Regenerable callus was induced in the basal 1-2 mm region of 2 to 5 day old seedlings. Plants were regenerated from the regenerable callus and grown to maturity. The frequency of regenerable callus f...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00238604

    authors: Chen H,Xu G,Loschke DC,Tomaska L,Rolfe BG

    更新日期:1995-03-01 00:00:00

  • Heading date gene, dth3 controlled late flowering in O. Glaberrima Steud. by down-regulating Ehd1.

    abstract::Heading date in rice is an important agronomic trait controlled by several genes. In this study, flowering time of variety Dianjingyou 1 (DJY1) was earlier than a near-isogenic line (named NIL) carried chromosome segment from African rice on chromosome 3S, when grown in both long-day (LD) and short-day (SD) conditions...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-011-1129-4

    authors: Bian XF,Liu X,Zhao ZG,Jiang L,Gao H,Zhang YH,Zheng M,Chen LM,Liu SJ,Zhai HQ,Wan JM

    更新日期:2011-12-01 00:00:00

  • An efficient adventitious shoot regeneration system for Zhanhua winter jujube (Zizyphus jujuba Mill.) using leaf explants.

    abstract::The direct induction of adventitious shoots from leaf explants obtained from adult plants of Zhanhua winter jujube, an elite variety of Zizyphus jujuba Mill., is reported. The percentage of leaf explants producing shoots and the average number of shoots per explant were significantly improved when 10-day-old leaves we...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-005-0920-5

    authors: Gu XF,Zhang JR

    更新日期:2005-03-01 00:00:00

  • Cloning and characterization of HsfA2 from Lily (Lilium longiflorum).

    abstract::Heat shock transcription factors (Hsfs) are the terminal components of the signal transduction chain mediating the activation of genes responsive to both heat stress and a large number of chemical stressors. This paper aims to clone Hsf from lily and characterize its function by analyses of mRNA expression, transactiv...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-010-0873-1

    authors: Xin H,Zhang H,Chen L,Li X,Lian Q,Yuan X,Hu X,Cao L,He X,Yi M

    更新日期:2010-08-01 00:00:00

  • Analyzing serial cDNA libraries revealed reactive oxygen species and gibberellins signaling pathways in the salt response of Upland cotton (Gossypium hirsutum L.).

    abstract:KEY MESSAGE:By comparing series full-length cDNA libraries stressed and control, the dynamic process of salt stress response in Upland cotton was studied, and reactive oxygen species and gibberellins signaling pathways were proposed. The Upland cotton is the most important fiber plant with highly salt tolerance. Howeve...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-015-1761-5

    authors: Shi G,Guo X,Guo J,Liu L,Hua J

    更新日期:2015-06-01 00:00:00

  • Inefficient expression of the DNA-binding domain of GAL4 in transgenic plants.

    abstract::The DNA-binding domain of the yeast transcriptional activator GAL4 was expressed in transgenic tobacco plants in order to attempt to obtain specific repression of reporter genes. Expression of the GUS and the NPTII gene was essentially the same regardless of the presence of a gene encoding a functional GAL4 DNA-bindin...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232920

    authors: Reichel C,Feltkamp D,Walden R,Steinbiß HH,Schell J,Rosahl S

    更新日期:1995-10-01 00:00:00