Plant regeneration from protoplasts of wheat (Triticum aestivum cv. Hartog).

abstract：:To improve turfgrasses using genetic engineering, we have developed a transformation system in turf-type tall fescue, one of the most important turfgrass species. Embryogenic cell cultures were established after callus induction from embryos of mature seed. The agarose-bead method with nurse cells was used to culture ...

journal_title：Plant cell reports

authors： Ha SB,Wu FS,Thorne TK

更新日期：1992-11-01 00:00:00

abstract：:Separation of stem proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis permitted classification of 17 celery varieties (Apium graveolens L.) into 7 groups. Several of these groups could be further subdivided into 11 subgroups with gel electrophoresis patterns of 4 isozyme systems. Classification from...

journal_title：Plant cell reports

authors： Quiros CF,McGrath M,Stites JL

更新日期：1987-04-01 00:00:00

abstract：:We have previously developed procedures for the efficient production of sweet orange (Citrus sinensis L. Osbeck) and Carrizo citrange (C. sinensis L. Osbeck×Poncirus trifoliata L. Raf.) transgenic plants using an Agrobacterium tumefaciens-mediated transformation and shoot tip grafting in vitro regeneration system. We ...

journal_title：Plant cell reports

authors： Peña L,Cervera M,Juárez J,Navarro A,Pina JA,Navarro L

更新日期：1997-09-01 00:00:00

abstract：KEY MESSAGE:TaUBA functions as a negative regulator of salt and drought stress response in transgenic Arabidopsis, either the UBA domain or the zinc finger domain is crucial for TaUBA's function. TaUBA (DQ211935), which is a UBA domain-containing protein in wheat, was cloned and functionally characterized. Southern blo...

journal_title：Plant cell reports

authors： Li X,Zhang SS,Ma JX,Guo GY,Zhang XY,Liu X,Bi CL

更新日期：2015-05-01 00:00:00

abstract：:Suspension cultures derived from Lycium chinense Miller seedlings produced significant amounts of a hepatoprotective cerebroside. Callus was induced from the stem of aseptic seedlings of L. chinense and maintained on MS solid media supplemented with 1.0 ppm 2,4-D and 0.1 ppm kinetin. Suspension cultures were establish...

journal_title：Plant cell reports

authors： Jang YP,Lee YJ,Kim YC,Huh H

更新日期：1998-12-01 00:00:00

abstract：:In an effort to develop a chemically inducible system for insect management, we studied production of Cry1Ab Bacillus thuringiensis (Bt) protein and control of the diamondback moth (DBM), Plutella xylostella L., in inducer-treated and untreated tissues of a broccoli line transformed with a PR-1a/cry1Ab expression cass...

journal_title：Plant cell reports

authors： Cao J,Bates SL,Zhao JZ,Shelton AM,Earle ED

更新日期：2006-06-01 00:00:00

abstract：:Carotenoid cleavage dioxygenases (CCDs) are involved in the production of diverse apocarotenoids including phytohormones, the visual molecules and the aromatic volatile compounds derived from carotenoids. Here, we examined the spatial expression of four of the CCD genes (AtCcd1, 4, 7 and 8) among the nine members of t...

journal_title：Plant cell reports

authors： Liang YS,Jeon YA,Lim SH,Kim JK,Lee JY,Kim YM,Lee YH,Ha SH

更新日期：2011-06-01 00:00:00

abstract：KEY MESSAGE:The Arabidopsis AP2/ERF family transcription factor AtERF15 is nuclear-localized and positively regulates ABA and stress responses. Abscisic acid (ABA) is a major plant hormone that controls the expression of hundreds genes involved in various aspects of plant growth and development, such as seed developmen...

journal_title：Plant cell reports

authors： Lee SB,Lee SJ,Kim SY

更新日期：2015-01-01 00:00:00

abstract：KEY MESSAGE:Phytophthora infestans resistant somatic hybrids of S. × michoacanum (+) S. tuberosum and autofused 4 x S. × michoacanum were obtained. Our material is promising to introgress resistance from S. × michoacanum into cultivated potato background. Solanum × michoacanum (Bitter.) Rydb. (mch) is a wild diploid (2...

journal_title：Plant cell reports

authors： Smyda P,Jakuczun H,Dębski K,Sliwka J,Thieme R,Nachtigall M,Wasilewicz-Flis I,Zimnoch-Guzowska E

更新日期：2013-08-01 00:00:00

abstract：KEY MESSAGE:A novel rice constitutive promoter (P OsCon1 ) was isolated. The molecular mechanism of the promoter activity was investigated. P OsCon1 could be used as an alternative constitutive promoter for crop transgenic engineering. Monocot constitutive promoter is an important resource for crop transgenic engineeri...

journal_title：Plant cell reports

authors： Li J,Xu RF,Qin RY,Ma H,Li H,Zhang YP,Li L,Wei PC,Yang JB

更新日期：2014-10-01 00:00:00

abstract：:Protoplasts isolated from cell suspensions of albinoMedicago borealis andM. sativa were fused chemically, using two methods, and electrically. Although a small scale method of chemical fusion gave the highest fusion frequency, electrofusion was the superior technique on the basis of throughput of green somatic hybrid ...

journal_title：Plant cell reports

authors： Gilmour DM,Davey MR,Cocking EC

更新日期：1989-05-01 00:00:00

abstract：:The production of cybrids, combining nucleus of a species with alien cytoplasmic organelles, is a valuable method used for improvement of various crops. Several citrus cybrids have been created by somatic hybridization. These genotypes are interesting models to analyze the impact of cytoplasmic genome change on nuclea...

journal_title：Plant cell reports

authors： Bassene JB,Froelicher Y,Navarro L,Ollitrault P,Ancillo G

更新日期：2011-06-01 00:00:00

abstract：:Mimosa tenuiflora (Willd.) Poiret (Leguminosae) was micropropagated throughin vitro culture of axillary buds on Murashige and Skoog (MS) medium. Shoot formation was achieved when the media were supplemented with 0.1 mg.L(-1) IAA + 3 mg.L(-1) KN.In vitro rooting of regenerated shoots was achieved when 0.1 mg.L(-1) KN w...

journal_title：Plant cell reports

authors： Villarreal ML,Rojas G

更新日期：1996-11-01 00:00:00

abstract：:Plant regeneration in Arachis pintoi was obtained via two developmental pathways: organogenesis and somatic embryogenesis. Organogenic callus cultures were initiated from pieces of leaf on MS medium supplemented with NAA or 2,4-D in combination with BA, KIN or 2iP. The most suitable combination for plant regeneration ...

journal_title：Plant cell reports

authors： Rey HY,Scocchi AM,Gonzalez AM,Mroginski LA

更新日期：2000-09-01 00:00:00

abstract：:The transit peptide sequence of ibAGP2 (TP2) was found to be capable of targeting protein into the chloroplast in the Arabidopsis protoplasts. TP2 was fused to a beta-glucuronidase (GUS) reporter gene and expressed in Arabidopsis under the control of the ibAGP2 promoter with the aim of dissecting the effect of the tra...

journal_title：Plant cell reports

authors： Kwak MS,Oh MJ,Paek KH,Shin JS,Bae JM

更新日期：2008-08-01 00:00:00

abstract：:The hybridity of eleven somatic hybrids between a diploidS. tuberosum and a diploidS. phureja clone could be verified because the parent karyotypes differed in their C-banding patterns. The hybrids were hypotetraploid and some carried structurally rearranged chromosomes and/or minute centric fragments. The nucleolar c...

journal_title：Plant cell reports

authors： Pijnacker LP,Ferwerda MA,Puite KJ,Schaart JG

更新日期：1989-02-01 00:00:00

abstract：:Microspore-derived embryos from Brassica napus L. were dried to less than 15% moisture and stored dry for a minimum of 7 days. Successful plant regeneration was observed when embryos at the cotyledonary stage of development were treated with 50 uM ABA for 7 days prior to desiccation. Solid agar or liquid medium gave s...

journal_title：Plant cell reports

authors： Senaratna T,Kott L,Beversdorf WD,McKersie BD

更新日期：1991-09-01 00:00:00

abstract：:Domain of Unknown Function 966 (DUF966) gene family was found in the protein family database, which consisted of seven genes in rice. The proteins encoded by these genes contained one or two highly conserved DUF966 domains. The available data of public microarray databases implied that these genes might play crucial r...

journal_title：Plant cell reports

authors： Luo C,Guo C,Wang W,Wang L,Chen L

更新日期：2014-02-01 00:00:00

abstract：:Jasmonates enhance the expression of various genes involved in terpenoid indole alkaloid (TIA) biosynthesis in Catharanthus roseus. We applied precursor feeding to our C. roseus suspensions to determine how methyl jasmonate (MJ) alters the precursor availability for TIA biosynthesis. C. roseus suspensions were induced...

journal_title：Plant cell reports

authors： Lee-Parsons CW,Royce AJ

更新日期：2006-06-01 00:00:00

abstract：:Hypocotyl explants of three leguminous forest tree species, Albizia amara, A. lucida and A. richardiana, have differentiated shoot buds on B5 basal medium. Maximum number of shoots per explant developed on basal medium augmented with 2,4-D (0.1 μM) in A. amara (2) and BA (10 μM) for both A. lucida (2) and A. richardia...

journal_title：Plant cell reports

authors： Tomar UK,Gupta SC

更新日期：1988-10-01 00:00:00

abstract：: An Agrobacterium tumefaciens-mediated transformation method has been developed for onions (Allium cepa L.) using immature embryos as the explant source. Transgenic plants were recovered from the open-pollinated onion cultivar Canterbury Longkeeper at a maximum transformation frequency from immature embryos of 2.7%. T...

journal_title：Plant cell reports

authors： Eady CC,Weld RJ,Lister CE

更新日期：2000-03-01 00:00:00

abstract：:Globe artichoke represents a natural source of phenolic compounds with dicaffeoylquinic acids along with their biosynthetic precursor chlorogenic acid (5-caffeoylquinic acid) as the predominant molecules. We report the isolation and characterization of a full-length cDNA and promoter of a globe artichoke p-coumaroyl e...

journal_title：Plant cell reports

authors： Moglia A,Comino C,Portis E,Acquadro A,De Vos RC,Beekwilder J,Lanteri S

更新日期：2009-06-01 00:00:00

abstract：:The cauliflower mosaic virus 35S (35S) and the enhanced 35S (E35S) promoters fused with maize alcohol dehydrogenase (Adh1) intron1 or maize shrunken locus (sh1) intronl along with maize Adh1 and rice actin (Act1) promoters fused to their respective first introns were tested for transient expression of the E.coli β-glu...

journal_title：Plant cell reports

authors： Chibbar RN,Kartha KK,Datla RS,Leung N,Caswell K,Mallard CS,Steinhauer L

更新日期：1993-07-01 00:00:00

abstract：:Plating rice anthers on a semisolid induction medium containing 250 or 500 mg/l colchicine for 24 or 48 h-incubations followed by transfer to colchicine-free medium and standard anther culture procedures resulted in overall 1.5- to 2.5- fold increases in doubled haploid green plant productions compared to control anth...

journal_title：Plant cell reports

authors： Alemanno L,Guiderdoni E

更新日期：1994-05-01 00:00:00

abstract：:A biolistic transformation procedure was used to transform embryogenic Pinus radiata tissue with constructs containing the Zea mays UBI1 (ubiquitin)-promoter followed by the P. radiata CAD (cinnamyl alcohol dehydrogenase) cDNA in sense or anti-sense orientation or in the form of an inverted-repeat. The effect of the d...

journal_title：Plant cell reports

authors： Wagner A,Phillips L,Narayan RD,Moody JM,Geddes B

更新日期：2005-05-01 00:00:00

abstract：:The variation among and within different populations of the regeneration ability from leaf, cotyledon and hypocotyl explants has been studied. A control population and two lines selected by their regeneration capacity from leaf explants were used. Significant differences among the plants of the control population,for ...

journal_title：Plant cell reports

authors： Molina RV,Nuez F

更新日期：1995-01-01 00:00:00

abstract：:Protoplasts isolated from mesophyll cells of Eruca sativa Lam., cultured on suitable medium, underwent sustained cell divisions to form calli. The plating efficiency was found to be 0.4%. The protoplast-derived calli subsequently produced plantlets through organogenesis (15.71%) and somatic embryogenesis (11.25%). Reg...

journal_title：Plant cell reports

authors： Sikdar SR,Chatterjee G,Das S,Sen SK

更新日期：1987-12-01 00:00:00

abstract：:The ample supply of O2 proved to be of great importance for berberine production in cell suspension culture ofThalictrum minus, as the specific O2 consumption rate of berberine-producing cells was twice as high as that of non-producing cells. Furthermore, berberine yield increased with increases in the volumetric O2 t...

journal_title：Plant cell reports

authors： Kobayashi Y,Fukui H,Tabata M

更新日期：1989-04-01 00:00:00

abstract：:Haploid plants were regenerated from cultured unfertilized ovaries of Hordeum vulgare L. (barley). Optimal response was obtained by the addition of 0.6 μM 4-chloro-2-methylphenoxyacetic acid (MCPA), 2.8 μM indole-3-acetic acid (IAA) and 4.4 μM 6-benzyladenine (BA) in the N6 medium. Further increase in the rate of call...

journal_title：Plant cell reports

authors： Castillo AM,Cistué L

更新日期：1993-01-01 00:00:00

abstract：:Somatic embroys and subsequent plant regeneration were obtained from isolated leaf epidermis of Gaillardia picta. Abaxial and adaxial epidermal peels (monolayer) from 45 days old aseptic seedlings were isolated and segments measuring 5 mm x 3 mm were cultured on B5 basal medium supplemented with various growth regulat...

journal_title：Plant cell reports

authors： Pillai KG,Rao IU,Rao IV,Ram HY

更新日期：1992-01-01 00:00:00