Abstract：
:The cauliflower mosaic virus 35S (35S) and the enhanced 35S (E35S) promoters fused with maize alcohol dehydrogenase (Adh1) intron1 or maize shrunken locus (sh1) intronl along with maize Adh1 and rice actin (Act1) promoters fused to their respective first introns were tested for transient expression of the E.coli β-glucuronidase (gus) reporter gene in cultured barley (Hordeum vulgare L) cells. The plasmids, carrying the respective promoterintron combinations to drive the gus fused to nopaline synthase (nos) terminator, were introduced into cultured barley cells using a particle gun. The rice Act1 promoter with its first intron gave the highest expression of all promoter intron combinations studied. This was followed by the E35S promoter and no significant differences were observed between the other two promoters tested. The rice actin promoter is now being used to drive selectable marker genes to obtain stably transformed cereal cells.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Chibbar RN,Kartha KK,Datla RS,Leung N,Caswell K,Mallard CS,Steinhauer Ldoi
10.1007/BF00236096subject
Has Abstractpub_date
1993-07-01 00:00:00pages
506-9issue
9eissn
0721-7714issn
1432-203Xjournal_volume
12pub_type
杂志文章abstract：:A protocol is described for rapid and large-scale propagation of the woody aromatic and medicinal shrub Vitex negundo by in vitro culture of nodal segments from mature plants. Of the three different cytokinins - N6-benzyladenine (BA), kinetin, and thidiazuron - evaluated as supplements to Murashige and Skoog (MS) medi...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050576
更新日期：1998-12-01 00:00:00
abstract：KEY MESSAGE:Sensitivity to Erysiphe in Noccaea praecox with low metal supply is related to the failure in enhancing SA. Cadmium protects against fungal-infection by direct toxicity and/or enhanced fungal-induced JA signaling. Metal-based defense against biotic stress is an attractive hypothesis on evolutionary advantag...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-013-1427-0
更新日期：2013-08-01 00:00:00
abstract：:Programmed cell death (PCD) is a process that occurs both in animals and in plants and is an essential element in developmental processes. Pollination is a key factor in fruit production and self-incompatibility is one of the main limiting factors of this process. PCD has recently been put forward as a possible cause ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-010-0845-5
更新日期：2010-06-01 00:00:00
abstract：:Somatic embryos and plantlets have been obtained in quite a direct way from mesophyll protoplasts isolated from androgenetic plants of two cultivars ("Loras" and "Tower") of Brassica napus. The procedure consists of four steps. Proembryos were induced in a medium supplemented with a cytokinin and an auxin at comparati...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00270237
更新日期：1982-10-01 00:00:00
abstract：KEY MESSAGE:Overexpression of SoSnRK2.1 improved drought tolerance and growth of tobacco plants. Sucrose non-fermenting1-related protein kinase 2 (SnRK2) is a key enzyme in regulating ABA signal transduction in plants, and it plays a significant role in response to multiple abiotic stresses. In this research, SoSnRK2.1...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-016-2004-0
更新日期：2016-09-01 00:00:00
abstract：:Hypocotyl explants of Albizzia julibrissin were placed on Gamborg's B5 medium supplemented with various levels of paclobutrazol, uniconazole, prohexadione calcium, or GA3. Callus formation was evident within one week after placement of the explants on the culture media. Green nodule-like structures protruded from the ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00235303
更新日期：1993-12-01 00:00:00
abstract：:Valencia orange [Citrus sinensis (L.) Osbeck] is the leading commercial citrus species in the world for processed juice products; however, the presence of thermostable pectin methylesterase (TSPME) reduces its juice quality. A long-term strategy of this work is to eliminate or greatly reduce TSPME activity in Valencia...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0952-x
更新日期：2005-10-01 00:00:00
abstract：:Cultivation of Catharanthus roseus hairy root cultures at different temperatures was found to have an effect on growth rate and indole alkaloid content as well as lipid composition. When lowering the temperature, the roots responded by increasing the degree of unsaturation of cellular lipids, which was mainly due to a...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00234368
更新日期：1992-07-01 00:00:00
abstract：:Polyclonal antibodies against a part of pea (Pisum sativum L.) LOXG protein have been raised to study the pattern of distribution of related lipoxygenases in pea carpels. The antiserum recognized three lipoxygenase polypeptides in carpels. One of them became undetectable 24 hours after fruit development induction, sug...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232465
更新日期：1996-04-01 00:00:00
abstract：:Leaf, rhizome, and stem explants of Helianthus smithii, a wild relative of the cultivated sunflower, have been tested for their morphogenic potential on media containing a range of hormonal combinations including α-naphthaleneacetic acid and 6-benzylaminopurine (0-2 mg/l). Somatic embryos appeared on the majority of t...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050303
更新日期：1997-07-01 00:00:00
abstract：:Protoclones and aggregate clones have been prepared from 5 callus strains of C. ternata chosen for their dihydrofuroquinoline-accumulating capacities in a population of well-established strains. The results show that it is possible to obtain cell lines which accumulate higher alkaloid contents than the highest alkaloi...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269565
更新日期：1987-10-01 00:00:00
abstract：:In maize, Mutator transposable elements are either active or silenced within the genome. In response to environmental stress, silenced Mutator elements could be reactivated, leading to changes in genome structure and gene function. However, there is no direct experimental evidence linking environmental stress and Muta...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-010-0922-9
更新日期：2010-12-01 00:00:00
abstract：:A protocol previously developed for B. napus microspore culture was modified to produce embryos from several lines of Brassica campestris. Bud size, genotype, media constituents, and incubation time and temperature were examined. Donor plants were grown in a growth cabinet at a day/night temperature of 10/5°C. Microsp...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00235072
更新日期：1992-06-01 00:00:00
abstract：:Globe artichoke represents a natural source of phenolic compounds with dicaffeoylquinic acids along with their biosynthetic precursor chlorogenic acid (5-caffeoylquinic acid) as the predominant molecules. We report the isolation and characterization of a full-length cDNA and promoter of a globe artichoke p-coumaroyl e...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-009-0695-1
更新日期：2009-06-01 00:00:00
abstract：:Ethylene emanation rates were assessed from leaf tissues of an embryogenic seed plant (Cycle 0) and regeneration cycle plants selected for enhanced embryogenesis (Cycles I, II and IV). In all experiments, ethylene was assessed from the basal 1 cm portion of the innermost leaf. Ethylene emanation was five-fold higher i...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00272059
更新日期：1989-03-01 00:00:00
abstract：:A procedure has been developed for the clonal propagation of Picrorhiza kurroa Royle ex Benth. through shoot tip culture. Murashige and Skoog's medium (1962) supplemented with kinetin (3.0 to 5.0 mg/l) supported rapid proliferation of multiple shoots from the explants. Addition of indole-3-acetic acid (1.0 mg/l) to th...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269324
更新日期：1988-05-01 00:00:00
abstract：:Transgenic papaya (Carica papaya L.) plants were regenerated from embryogenic cultures that were cocultivated with a disarmed C58 strain of Agrobacterium tumefaciens containing one of the following binary cosmid vectors: pGA482GG or pGA482GG/cpPRV-4. The T-DNA region of both binary vectors includes the chimeric genes ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00237128
更新日期：1993-03-01 00:00:00
abstract：:The AtGRP5 gene from Arabidopsis thaliana encodes a glycine-rich protein which has a major activity in protoderm-derived cells and is expressed in cells that undergo the first anatomical modifications leading to somatic embryo development. It has been previously demonstrated that its minimum promoter is 316 bp long in...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-010-0835-7
更新日期：2010-05-01 00:00:00
abstract：:This article describes conditions to optimize the yield of viable protoplasts from callus tissue of Asparagus densiflorus cv. Sprengeri and their subsequent regeneration into plantlets. Callus tissue was initiated by culturing spear sections (5-7 mm) on Murashige and Skoog (MS) medium supplemented with 0.8% (wt/vol) B...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050364
更新日期：1997-12-01 00:00:00
abstract：:Tissue culture methods for improvement of cotton has lagged seriously compared to other major crops. A method for regeneration of cotton which includes a morphogenetically competent cell suspension was needed to facilitate selection of stress-resistant variants and gene manipulation. Preliminary screening of eight str...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00268487
更新日期：1987-06-01 00:00:00
abstract：KEY MESSAGE:By comparing series full-length cDNA libraries stressed and control, the dynamic process of salt stress response in Upland cotton was studied, and reactive oxygen species and gibberellins signaling pathways were proposed. The Upland cotton is the most important fiber plant with highly salt tolerance. Howeve...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-015-1761-5
更新日期：2015-06-01 00:00:00
abstract：:Taro (Colocasia esculenta) is one of the most important crops in the Pacific Islands, however, taro yields have been declining in Hawaii over the past 30 years partly due to diseases caused by oomycete and fungal pathogens. In this study, an efficient Agrobacterium tumefaciens-mediated transformation method for taro i...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-008-0519-8
更新日期：2008-05-01 00:00:00
abstract：:Randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were used to analyse the genetic diversity of Portuguese Prunus dulcis cultivars and their relationship to important foreign cultivars. Of the primers tested, 6 (out of 60) RAPD and 5 (out of 18) ISSR primers were selected for th...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-003-0659-9
更新日期：2003-08-01 00:00:00
abstract：:Seeds are heterogeneous storage reserves with wide array of storage compounds that include various soluble carbohydrates, starch polymer, storage proteins and lipids. These stored reserves comprise 70% of the world's caloric intake in the form of food and animal feed produced through sustainable agriculture, which con...
journal_title：Plant cell reports
pub_type： 社论
doi：10.1007/s00299-017-2135-y
更新日期：2017-05-01 00:00:00
abstract：:To study the developmental transition of chloroplasts from C(3) to C(4) photosynthesis in the terrestrial single-cell C(4) species Bienertia sinuspersici, a regeneration protocol was developed. Stem explant material developed callus either with or without red nodular structures (RNS) when cultured on Murashige-Skoog (...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-011-1067-1
更新日期：2011-08-01 00:00:00
abstract：:The hybridity of eleven somatic hybrids between a diploidS. tuberosum and a diploidS. phureja clone could be verified because the parent karyotypes differed in their C-banding patterns. The hybrids were hypotetraploid and some carried structurally rearranged chromosomes and/or minute centric fragments. The nucleolar c...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00716844
更新日期：1989-02-01 00:00:00
abstract：:Cotyledon explants from zygotic embryos of Panax ginseng produced somatic embryos on Murashige and Skoog basal medium without growth regulators. Somatic embryos developed directly from epidermal cells at the cotyledon base. Somatic embryos were always formed from the side of the cotyledon opposite to the one attached ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050331
更新日期：1997-10-01 00:00:00
abstract：:Cotyledon explants of muskmelon (Cucumis melo L., cv. Amarillo Oro) seedlings were co-cultivated with disarmed Agrobacterium tumefaciens strain LBA4404 that contained the binary vector plasmid pBI121.1. The T-DNA region of this binary vector contains the Nopaline synthase/neomycin phosphotransferase II (NPTII) chimeri...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00239881
更新日期：1994-01-01 00:00:00
abstract：:A system to regenerate fertile rice (Oryza sativa L.) plants (both indica and japonica varieties) from protoplasts isolated from anther-derived embryogenic haploid suspension cultures has been established. Green plants were regenerated from protoplast-derived cell clusters five months after suspension culture initiati...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232422
更新日期：1992-12-01 00:00:00
abstract：: A novel procedure has been developed to produce rice (Oryza sativa L.) tolerant to the herbicide phosphinothricin (PPT) by means of in vitro selection. First, sublethal and lethal concentrations of PPT on 7-day-old seedlings were determined and morphogenetic events in response to the PPT treatment evaluated. Differen...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990000276
更新日期：2000-12-01 00:00:00
