The tissue expression pattern of the AtGRP5 regulatory region is controlled by a combination of positive and negative elements.

abstract：KEY MESSAGE:Overexpression of Withania somnifera SGT gene (WssgtL3.1) in transgenic Arabidopsis improves various agronomic and physiological traits and alters conjugated sterol levels to mitigate the effect of salt stress. Sterols are essential constituents of cell membranes that are involved in several biological func...

journal_title：Plant cell reports

authors： Mishra MK,Tiwari S,Misra P

更新日期：2021-02-01 00:00:00

abstract：KEY MESSAGE:A plant-derived 0.3 kb constitutive promoter was obtained from AtTCTP expression analysis, and successfully applied to the expression of a selectable marker gene for production of transgenic creeping bentgrass plants. The isolation and use of an efficient promoter is essential to develop a vector system for...

journal_title：Plant cell reports

authors： Han YJ,Kim YM,Hwang OJ,Kim JI

更新日期：2015-02-01 00:00:00

abstract：:Plant regeneration in Arachis pintoi was obtained via two developmental pathways: organogenesis and somatic embryogenesis. Organogenic callus cultures were initiated from pieces of leaf on MS medium supplemented with NAA or 2,4-D in combination with BA, KIN or 2iP. The most suitable combination for plant regeneration ...

journal_title：Plant cell reports

authors： Rey HY,Scocchi AM,Gonzalez AM,Mroginski LA

更新日期：2000-09-01 00:00:00

abstract：:A simple and reliable method was developed for isolating pollen nuclei from Brassica napus and Triticum aestivum for DNA analysis using flow cytometry. The nuclei were released from pollen by ultrasonic treatment. The isolated nuclei following filtration through nylon mesh and a purification procedure were suitable fo...

journal_title：Plant cell reports

authors： Pan G,Zhou Y,Fowke LC,Wang H

更新日期：2004-10-01 00:00:00

abstract：:Molecular analysis of gene expression differences between green and red lettuce leaves was performed using the SSH method. BlastX comparisons of subtractive expressed sequence tags (ESTs) indicated that 7.6% of clones encoded enzymes involved in secondary metabolism. Such clones had a particularly high abundance of fl...

journal_title：Plant cell reports

authors： Park JS,Choung MG,Kim JB,Hahn BS,Kim JB,Bae SC,Roh KH,Kim YH,Cheon CI,Sung MK,Cho KJ

更新日期：2007-04-01 00:00:00

abstract：:The regeneration of somatic seedlings from selected 100-year-old cork oak trees is reported. The induction of somatic embryogenesis from leaves of epicormic shoots was significantly affected by genotype, harvesting time and their interaction. Leaves from all five selected trees produced somatic embryos when the segmen...

journal_title：Plant cell reports

authors： Hernández I,Celestino C,Alegre J,Toribio M

更新日期：2003-04-01 00:00:00

abstract：:A cDNA clone encoding an ADP-ribosylation factor from potato (Solanum tuberosum L.) was isolated. The nucleotide and deduced amino acid sequences show high homology to known ADP-ribosylation factor sequences from Arabidopsis, yeast, cow and man. In northern blot experiments, all tissues analysed showed expression of t...

journal_title：Plant cell reports

authors： Szopa J,Müller-Röber B

更新日期：1994-12-01 00:00:00

abstract：:A new method of estimating the amount of berberine released from minute cell colonies of Thalictrum minus has been devised to facilitate the selection of high berberine-producing cell lines. In this system, cell aggregates obtained from a cell suspension culture are grown on small pieces of an agar culture medium and ...

journal_title：Plant cell reports

authors： Suzuki T,Yoshioka T,Hara Y,Tabata M,Fujita Y

更新日期：1987-06-01 00:00:00

abstract：:The effects of NaCl, feeder cells and the embedding of protoplasts in calcium alginate have been investigated in an attempt to improve culture conditions of recalcitrant sugar beet (Beta vulgaris L.) mesophyll protoplasts. While the use of NaCl in all instances proved detrimental to protoplast development, the other t...

journal_title：Plant cell reports

authors： Hall RD,Pedersen C,Krens FA

更新日期：1993-04-01 00:00:00

abstract：:Conditions necessary for the isolation and culture of protoplasts from suspension cultures of sugar, fodder and garden beets were investigated. Good yields of protoplasts were obtained by treating cells with a mixture of cellulase, Macerozyme and Driselase enzymes. Nutritional requirements of beet protoplasts were fou...

journal_title：Plant cell reports

authors： Bhat SR,Ford-Lloyd BV,Callow JA

更新日期：1985-12-01 00:00:00

abstract：:A biolistic transformation procedure was used to transform embryogenic Pinus radiata tissue with constructs containing the Zea mays UBI1 (ubiquitin)-promoter followed by the P. radiata CAD (cinnamyl alcohol dehydrogenase) cDNA in sense or anti-sense orientation or in the form of an inverted-repeat. The effect of the d...

journal_title：Plant cell reports

authors： Wagner A,Phillips L,Narayan RD,Moody JM,Geddes B

更新日期：2005-05-01 00:00:00

abstract：:Various inhibitors of polyamine biosynthesis were used to study the role of polyamines in DNA synthesis and cell division in suspension cultures of Catharanthus roseus (L.) G. Don. Arginine decarboxylase (ADC; EC 4.1.1.19) was the major enzyme responsible for putrescine production. DL α-difluoromethylarginine inhibite...

journal_title：Plant cell reports

authors： Minocha R,Minocha SC,Komamine A,Shortle WC

更新日期：1991-06-01 00:00:00

abstract：:Significantly higher than normal mitotic index (MI) values were induced in Petunia cell suspensions following treatments with colchicine, aphidicolin, drastic medium replacement, or a sequential application of aphidicolin and colchicine. This last treatment yielded the highest MI values: cells incubated with 30 μg/ml ...

journal_title：Plant cell reports

authors： Guri A,Zelcer A,Izhar S

更新日期：1984-12-01 00:00:00

abstract：:Emission and plant uptake of atmospheric nitrogen oxides (NO + NO(2)) significantly influence regional climate change by regulating the oxidative chemistry of the lower atmosphere, species composition and the recycling of carbon and nutrients, etc. Plant uptake of nitrogen dioxide (NO(2)) is concentration-dependent an...

journal_title：Plant cell reports

authors： Hu Y,Sun G

更新日期：2010-10-01 00:00:00

abstract：KEY MESSAGE:Our studies indicate a potential important elicitor candidate which can aid in the fight against a worldwide disease, rice blast. In this study, we report the purification, identification, characterization, and gene cloning of a novel hypersensitive response-inducing protein elicitor (MoHrip2) secreted from...

journal_title：Plant cell reports

authors： Chen M,Zhang C,Zi Q,Qiu D,Liu W,Zeng H

更新日期：2014-11-01 00:00:00

abstract：:LITHOSPERMUM ERYTHRORHIZON: , which are capable of producing red pigments, have been established. The red pigments were formed on the stems of L. erythrorhizon shoots cultured both on solid and in liquid media without phytohormones at 25 °C in the dark. Thin-layer chromatography, high-performance liquid chromatography...

journal_title：Plant cell reports

authors： Touno K,Harada K,Yoshimatsu K,Yazaki K,Shimomura K

更新日期：2000-11-01 00:00:00

abstract：:Through the use of Capsicum chromoplast membranes, we report for the first time the direct methylation of γ-tocopherol into α-tocopherol in the presence of S-adenosylmethionine. Furthermore the S-adenosylmethionine: γ-tocopherol methyltransferase activity has been solubilized. On a protein basis, the activity recovere...

journal_title：Plant cell reports

authors： Camara B,d'Harlingue A

更新日期：1985-02-01 00:00:00

abstract：KEY MESSAGE:Thirty genes involved in GA and ABA metabolism and signalling were identified, and the expression profiles indicated that they play crucial roles in the bud activity-dormancy transition in tea plants. Gibberellin (GA) and abscisic acid (ABA) are fundamental phytohormones that extensively regulate plant grow...

journal_title：Plant cell reports

authors： Yue C,Cao H,Hao X,Zeng J,Qian W,Guo Y,Ye N,Yang Y,Wang X

更新日期：2018-03-01 00:00:00

abstract：:High yields of viable protoplasts were obtained by enzymatic treatment from cotyledonary leaves of various greenhouse grown Phaseolus vulgaris L. cultivars. The protoplasts divided and formed cell clusters in a liquid medium. Early transfer before 10 days in the same medium was necessary for the development of cell co...

journal_title：Plant cell reports

authors： Crepy L,Barros LM,Valente VR

更新日期：1986-04-01 00:00:00

abstract：:A method has been devised which allows the rapid production of fine suspension cultures of small aggregate size from suspension cultures of large average aggregate size, such as those of Capsicum frutescens. The method, which uses a Waring blender for aseptic homogenisation of cultures, has also been shown to be effec...

journal_title：Plant cell reports

authors： Williams PD,Wilkinson AK,Lewis JA,Black GM,Mavituna F

更新日期：1988-10-01 00:00:00

abstract：:A procedure to produce fruits from cultured shoot tips of Cucumis sativus L. cultivar 'Marketmore-76' in vitro is described. Four-week-old shoot tips, derived from sterile germinated seedlings on a MS medium, were cultured in a 3.8-1 Mason jar using an automated plant culture system. Tips readily generated roots, leav...

journal_title：Plant cell reports

authors： Tisserat B,Galletta PD

更新日期：1993-11-01 00:00:00

abstract：:Chimeric constructs consisting of the gus coding region fused downstream of promoterun-translated leader sequences from the tobacco osmotin and PR-S genes, the potato proteinase inhibitor 2 gene (pin2), and the cauliflower mosaic virus (CaMV) 35S promoter were biolistically transferred into sugarbeet suspension cells....

journal_title：Plant cell reports

authors： Ingersoll JC,Heutte TM,Owens LD

更新日期：1996-08-01 00:00:00

abstract：:Most infections of plant roots are initiated in the region of elongation; the mechanism for this tissue-specific localization pattern is unknown. In alfalfa expressing PsUGT1 antisense mRNA under the control of the cauliflower mosaic virus (CaMV) 35S promoter, the cell cycle in roots is completed in 48 h instead of 24...

journal_title：Plant cell reports

authors： Woo HH,Hirsch AM,Hawes MC

更新日期：2004-07-01 00:00:00

abstract：:Recently, five novel fluorescent proteins have been isolated from non-bioluminescent species of reef-coral organisms and have been made available through ClonTech. They are AmCyan, AsRed, DsRed, ZsGreen and ZsYellow. These proteins are valuable as reporters for transformation because they do not require a substrate or...

journal_title：Plant cell reports

authors： Wenck A,Pugieux C,Turner M,Dunn M,Stacy C,Tiozzo A,Dunder E,van Grinsven E,Khan R,Sigareva M,Wang WC,Reed J,Drayton P,Oliver D,Trafford H,Legris G,Rushton H,Tayab S,Launis K,Chang YF,Chen DF,Melchers L

更新日期：2003-11-01 00:00:00

abstract：:The in vivo nitrate reductase activity in 8 day old dark-grown sugarcane callus was over three fold that of the light-grown callus. NADH (0.3 mM) in the reaction system, increased the in vivo nitrate reductase activity by more than two fold both in the dark- and the light-grown callus tissues. The NADH dependence of n...

journal_title：Plant cell reports

authors： Khan BM,Dwivedi UN,Rawal SK,Mascarenhas AF

更新日期：1984-08-01 00:00:00

abstract：:An endo-pectate lyase (PL; EC 4.2.2.2), originally cloned fiom the phytopathogenic bacterium Erwinia chrysanthemi EC16, was expressed in recA (-) E. coli strain DK1, purified to a single band by isoelectric focusing and used to induce berberine production in established plant suspension cultures of Thalictrum minus L....

journal_title：Plant cell reports

authors： Smolko DD,Peretti SW

更新日期：1994-12-01 00:00:00

abstract：:Cell structures were compared between alkaloid-producing and non-producing cell cultures of Coptis japonica and Thalictrum minus by electron microscopic observation. In alkaloid-producing cells of C. japonica, prior to the onset of alkaloid synthesis, the vacuoles showed a greater volume than in non-producing cells. T...

journal_title：Plant cell reports

authors： Yamamoto H,Nakagawa K,Fukui H,Tabata M

更新日期：1986-02-01 00:00:00

abstract：KEY MESSAGE:Transcript profiling during the early induction phase of oil palm tissue culture and RNAi studies in a model somatic embryogenesis system showed that EgENOD93 expression is essential for somatic embryogenesis. Micropropagation of oil palm through tissue culture is vital for the generation of superior and un...

journal_title：Plant cell reports

authors： Chan PL,Rose RJ,Abdul Murad AM,Zainal Z,Ong PW,Ooi LC,Low EL,Ishak Z,Yahya S,Song Y,Singh R

更新日期：2020-11-01 00:00:00

abstract：KEY MESSAGE:The zeaxanthin epoxidase gene ( MsZEP ) was cloned and characterized from alfalfa and validated for its function of tolerance toward drought and salt stresses by heterologous expression in Nicotiana tabacum. Zeaxanthin epoxidase (ZEP) plays important roles in plant response to various environment stresses d...

journal_title：Plant cell reports

authors： Zhang Z,Wang Y,Chang L,Zhang T,An J,Liu Y,Cao Y,Zhao X,Sha X,Hu T,Yang P

更新日期：2016-02-01 00:00:00

abstract：:Human growth hormone (hGH), a pituitary-derived polypeptide, evidences a wide range of biological functions, including protein synthesis, cell proliferation, and metabolism. A synthetic hGH gene (shGH) has been synthesized on the basis of plant-optimized codon usage via an overlap PCR strategy and located in a plant e...

journal_title：Plant cell reports

authors： Kim TG,Baek MY,Lee EK,Kwon TH,Yang MS

更新日期：2008-05-01 00:00:00