Demonstration and solubilization of S-adenosylmethionine: γ-tocopherol methyltransferase from Capsicum chromoplasts.

abstract：:The stability of foreign protein production in genetically engineered plant cells was studied. A cultured tobacco cell line was transformed with a chimeric molecule carrying a bacterial gene, ß-glucuronidase (GUS), under plant regulatory sequences. The specific GUS activity was monitored for 294 days with ten independ...

journal_title：Plant cell reports

authors： Gao J,Lee JM,An G

更新日期：1991-12-01 00:00:00

abstract：:Optimum conditions for the isolation and culture of protoplasts from high anthocyanin-producing callus of sweet potato (Ipomoea batatas) were investigated. Growth phase of callus and the ratio of callus-enzyme solution affected the yield of protoplasts. Composition of the medium and protoplast density were examined fo...

journal_title：Plant cell reports

authors： Nishimaki T,Nozue M

更新日期：1985-10-01 00:00:00

abstract：KEY MESSAGE:The novel sunflower gene HaGLP1 is the first germin-like protein characterized from the family Asteraceae. It alters the host redox status and confers protection against Sclerotinia sclerotiorum and Rhizoctonia solani. Germin-like proteins (GLPs) are a large, diverse and ubiquitous family of plant glycoprot...

journal_title：Plant cell reports

authors： Beracochea VC,Almasia NI,Peluffo L,Nahirñak V,Hopp EH,Paniego N,Heinz RA,Vazquez-Rovere C,Lia VV

更新日期：2015-10-01 00:00:00

abstract：:The growth of Distichlis spicata suspension cultures in LS medium without NaCl was inhibited 54% by 2 mM proline. In medium containing 260 mM NaCl, 10 mM proline inhibited growth by only 22%. The uptake and metabolism of 10 mM L-[1-(13)C] proline was followed by (13)C NMR and ninhydrin analyses of suspensions cultured...

journal_title：Plant cell reports

authors： Rodriguez MM,Heyser JW

更新日期：1988-08-01 00:00:00

abstract：KEY MESSAGE:Bacterial wilt resistant somatic hybrids were obtained via protoplast fusion between potato and eggplant and three types of nuclear genomes were identified in the hybrids through GISH and SSR analysis. ABSTRACT:Cultivated potato (Solanum tuberosum L.) lacks resistance to bacterial wilt caused by Ralstonia ...

journal_title：Plant cell reports

authors： Yu Y,Ye W,He L,Cai X,Liu T,Liu J

更新日期：2013-11-01 00:00:00

abstract：:The objective of this study was to correlate the time that DNA synthesis first occurs in haploid microspores of barley with cell cycle and plant morphological stages and to subsequently assess the influence of pretreatments on DNA synthesis at different stages of microspore development. Spikes with microspores in earl...

journal_title：Plant cell reports

authors： Shim YS,Kasha KJ

更新日期：2003-07-01 00:00:00

abstract：: Mature ovules of Dianthus (Caryophyllaceae) were histologically observed by clearing and serial sectioning to characterize the cells of the embryo sac. The results show that the mature embryo sac was located deep inside the hemitropous ovule due to thick nucellar tissue at the micropylar region. For the isolation of ...

journal_title：Plant cell reports

authors： Hoshino Y,Nishino E,Mii M

更新日期：2000-04-01 00:00:00

abstract：: Cells of a suspension culture of Doritaenopsis cv. New Toyohashi were placed in a mixture of 2 M glycerol and 0.4 M sucrose for 15 min at room temperature and then dehydrated with a vitrification solution (PVS2) for 1-3 h on ice and plunged into liquid nitrogen. The highest viability (64% by 2,3,5-triphenyltetrazoliu...

journal_title：Plant cell reports

authors： Tsukazaki H,Mii M,Tokuhara K,Ishikawa K

更新日期：2000-12-01 00:00:00

abstract：:A simple method using molecular hybridization was devised to quantitatively measure chloroplast DNA synthesis in vivo. Total cellular DNA isolated from Nicotiana tabacum suspension cells, labeled with (3)H-thymidine, was hybridized to nitrocellulose membrane-bound cloned chloroplast DNA (ct DNA) fragments. Colorless, ...

journal_title：Plant cell reports

authors： Cannon G,Heinhorst S,Siedlecki J,Weissbach A

更新日期：1985-04-01 00:00:00

abstract：KEY MESSAGE:MicroRNAs have higher expression stability than protein-coding genes in B. napus seeds and are therefore good reference genes for miRNA and mRNA RT-qPCR analysis. Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) has become the "gold standard" to gain insight into function of ...

journal_title：Plant cell reports

authors： Machado RD,Christoff AP,Loss-Morais G,Margis-Pinheiro M,Margis R,Körbes AP

更新日期：2015-07-01 00:00:00

abstract：:The rooting capacities of tips of seedling, juvenile and mature shoots of Sequoiadendron giganteum were compared on different rooting media (inductive and expressive media) after passage on an elongating medium. None of the cuttings rooted when continuously kept on medium containing the auxin NAA and vitamin D2. Perox...

journal_title：Plant cell reports

authors： Berthon JY,Boyer N,Gaspar T

更新日期：1987-10-01 00:00:00

abstract：:Shoot and leaf segments of a non-regenerable Medicago sativa L. genotype were cocultivated with the "shooty" mutant of Agrobacterium tumefaciens carrying the pGV 2206 plasmid. Transformed callus lines were selected and regenerated on the hormone free B5 medium. Southern blot analysis demonstrated integration of T-DNA ...

journal_title：Plant cell reports

authors： Kuchuk N,Komarnitski I,Shakhovsky A,Gleba Y

更新日期：1990-04-01 00:00:00

abstract：:In order to construct transgenic rice plant with an introduced oryzacystatin (OC)-β-glucuronidase (GUS) fusion gene, we first introduced it into rice protoplasts by electroporation, together with a marker gene conferring hygromycinresistance (pUC-HPH). In a transient assay using the transfected protoplasts, both OC an...

journal_title：Plant cell reports

authors： Hosoyama H,Irie K,Abe K,Arai S

更新日期：1995-12-01 00:00:00

abstract：:Protoplasts isolated from mesophyll cells of Eruca sativa Lam., cultured on suitable medium, underwent sustained cell divisions to form calli. The plating efficiency was found to be 0.4%. The protoplast-derived calli subsequently produced plantlets through organogenesis (15.71%) and somatic embryogenesis (11.25%). Reg...

journal_title：Plant cell reports

authors： Sikdar SR,Chatterjee G,Das S,Sen SK

更新日期：1987-12-01 00:00:00

abstract：:Companion cells are metabolically active and functionally specialized cells that behave as terminals for the transport of materials between phloem and the surrounding tissue. Although previous research has clarified the distinct function of companion cells, it is still largely unknown how plants establish and maintain...

journal_title：Plant cell reports

authors： Tsuwamoto R,Harada T

更新日期：2011-09-01 00:00:00

abstract：:The activity of a predicted promoter, PMC8, from Milk vetch dwarf virus was evaluated by comparing it with the cauliflower mosaic virus 35S RNA promoter (P35S) and PNCR, a promoter from Soybean chlorotic mottle virus. When the GUS fusion gene was introduced into tobacco, PMC8 showed a similar expression profile to P35...

journal_title：Plant cell reports

authors： Shirasawa-Seo N,Sano Y,Nakamura S,Murakami T,Gotoh Y,Naito Y,Hsia CN,Seo S,Mitsuhara I,Kosugi S,Ohashi Y

更新日期：2005-06-01 00:00:00

abstract：:Stem sections from shoot cultures maintained in vitro were used to produce transgenic plants of the potato, Solanum tuberosum L. cv. 'Russet Burbank'. Stem internode pieces inoculated with Agrobacterium tumefaciens containing coat protein genes from potato virus X and potato virus Y, produced shoots with a frequency o...

journal_title：Plant cell reports

authors： Newell CA,Rozman R,Hinchee MA,Lawson EC,Haley L,Sanders P,Kaniewski W,Tumer NE,Horsch RB,Fraley RT

更新日期：1991-05-01 00:00:00

abstract：:Processes involved in transformation of regenerable soybean (Glycine max (L.) Merrill) immature zygotic cotyledons were studied by assaying the transient expression of the β-glucuronidase gene driven by the 35S promoter and terminated at the 3' end by the soybean 7S storage protein gene. The plasmid containing the chi...

journal_title：Plant cell reports

authors： Moore PJ,Moore AJ,Collins GB

更新日期：1994-07-01 00:00:00

abstract：:A simple and reliable method was developed for isolating pollen nuclei from Brassica napus and Triticum aestivum for DNA analysis using flow cytometry. The nuclei were released from pollen by ultrasonic treatment. The isolated nuclei following filtration through nylon mesh and a purification procedure were suitable fo...

journal_title：Plant cell reports

authors： Pan G,Zhou Y,Fowke LC,Wang H

更新日期：2004-10-01 00:00:00

abstract：KEY MESSAGE:By comparing series full-length cDNA libraries stressed and control, the dynamic process of salt stress response in Upland cotton was studied, and reactive oxygen species and gibberellins signaling pathways were proposed. The Upland cotton is the most important fiber plant with highly salt tolerance. Howeve...

journal_title：Plant cell reports

authors： Shi G,Guo X,Guo J,Liu L,Hua J

更新日期：2015-06-01 00:00:00

abstract：:Cryopreservation has been shown to improve the frequency of virus elimination - specifically cucumber mosaic virus and banana streak virus - from banana ( Musa spp.) plants. To understand the mode of action of cryopreservation for the eradication of viral particles, we examined the ultrastructure of meristem tips at e...

journal_title：Plant cell reports

authors： Helliot B,Swennen R,Poumay Y,Frison E,Lepoivre P,Panis B

更新日期：2003-03-01 00:00:00

abstract：:Most perennial plants must pass through a long juvenile phase of vegetative development before they are capable of flowering. We have developed a method specifying inflorescence proliferation to bypass juvenility and maintain the adult phase. Bamboo ( Bambusa edulis) inflorescences were amplified by incubation in Mura...

journal_title：Plant cell reports

authors： Lin CS,Chen CT,Lin CC,Chang WC

更新日期：2003-06-01 00:00:00

abstract：:The full-length cDNAs of eight S ribonucleases (S-RNases) were cloned from stylar RNA of European pear cultivars that could not be characterized by the cleaved amplified polymorphic sequences (CAPS) marker system for genotyping European pear cultivars harboring nine S alleles Sa, Sb, Sd, Se, Sh, Sk, Sl, Sq, and Sr. Co...

journal_title：Plant cell reports

authors： Moriya Y,Yamamoto K,Okada K,Iwanami H,Bessho H,Nakanishi T,Takasaki T

更新日期：2007-03-01 00:00:00

abstract：:Morphologically normal green plants have reproducibly been regenerated from protoplasts of an Australian wheat (Triticum aestivum cv. Hartog). The protoplasts were isolated from fine embryogenic suspension cultures which were initiated from embryogenic callus. Protoplasts were incubated in a modified liquid MS medium ...

journal_title：Plant cell reports

authors： He DG,Yang YM,Scott KJ

更新日期：1992-02-01 00:00:00

abstract：:A protocol for protoplast isolation was developed and tested with five Capsicum genotypes representing two cultivated species, C. annuum and C. chinense. Key variables included growth conditions for source plants and the concentration of mannitol used as osmoticum. Protoplasts isolated from each of the genotypes becam...

journal_title：Plant cell reports

authors： Murphy JF,Kyle MM

更新日期：1994-04-01 00:00:00

abstract：:The tobacco cell line TX1 (Nicotiana tabacum) accumulates up to 10 mg cinnamoyl putrescines/g dry weight. This level was further increased under various growth limiting conditions. The negative effect of accumulated phosphate on the optimal expression of cinnamoyl putrescine biosynthesis was prevented by the fedbatch ...

journal_title：Plant cell reports

authors： Schiel O,Jarchow-Redecker K,Piehl GW,Lehmann J,Berlin J

更新日期：1984-02-01 00:00:00

abstract：:Significantly higher than normal mitotic index (MI) values were induced in Petunia cell suspensions following treatments with colchicine, aphidicolin, drastic medium replacement, or a sequential application of aphidicolin and colchicine. This last treatment yielded the highest MI values: cells incubated with 30 μg/ml ...

journal_title：Plant cell reports

authors： Guri A,Zelcer A,Izhar S

更新日期：1984-12-01 00:00:00

abstract：:Separation of stem proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis permitted classification of 17 celery varieties (Apium graveolens L.) into 7 groups. Several of these groups could be further subdivided into 11 subgroups with gel electrophoresis patterns of 4 isozyme systems. Classification from...

journal_title：Plant cell reports

authors： Quiros CF,McGrath M,Stites JL

更新日期：1987-04-01 00:00:00

abstract：:Recent developments in modern biotechnology such as the use of RNA interference (RNAi) have broadened the scope of crop genetic modification. RNAi strategies have led to significant achievements in crop protection against biotic and abiotic stresses, modification of plant traits, and yield improvement. As RNAi-derived...

journal_title：Plant cell reports

authors： Darsan Singh JK,Mat Jalaluddin NS,Sanan-Mishra N,Harikrishna JA

更新日期：2019-12-01 00:00:00

abstract：:Factors involved in promoting caulogenesis from hypocotyl explants of Phyllanthus fraternus were studied. Hypocotyl explants were cultured on B5 medium supplemented with 2,4-D or NAA in the presence and absence of BAP (at concentrations 0, 10(-7), 10(-6) and 10(-5)M). Adventitious shoots differentiated from callus dev...

journal_title：Plant cell reports

authors： Rajasubramaniam S,Pardha Saradhi P

更新日期：1994-08-01 00:00:00