Abstract:
:The growth of Distichlis spicata suspension cultures in LS medium without NaCl was inhibited 54% by 2 mM proline. In medium containing 260 mM NaCl, 10 mM proline inhibited growth by only 22%. The uptake and metabolism of 10 mM L-[1-(13)C] proline was followed by (13)C NMR and ninhydrin analyses of suspensions cultured in the presence of 0 or 260 mM NaCl. Uptake of 85 to 92% of the exogenous proline occurred within 72 h in all media. In 10 mM proline and no NaCl, cellular proline reached a maximm of 51.5 μmoles/g FW compared to 1.9 μmoles/g FW in suspensions not grown on proline. In medium containing 260 mM NaCl and proline, cellular proline reached 59-65 μmoles/g FW compared to 30-40 μmoles/g FW in controls grown without proline. The (13)C-label in the proline-C1 was either retained in proline or disappeared, presumably released as carbon dioxide, by catabolism through the TCA cycle. Since no metabolite of (13)C-proline was detected by NMR, proline was considered to be the molecule which inhibited the suspension culture growth.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Rodriguez MM,Heyser JWdoi
10.1007/BF00269924subject
Has Abstractpub_date
1988-08-01 00:00:00pages
305-8issue
5eissn
0721-7714issn
1432-203Xjournal_volume
7pub_type
杂志文章abstract::Low initiation frequency is one of the main barriers in applying somatic embryogenesis to the clonal production of Pinus species. Factors affecting initiation, including basal medium, plant growth regulators, and Phytagel concentration, have been investigated in loblolly pine (Pinus taeda L.). BM1 basal medium proved ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050396
更新日期:1998-02-01 00:00:00
abstract::Cultured Catharanthus roseus cells exhibit transmembrane ferricyanide reduction through a plasma membrane redox system which may be associated with proton translocation. Evidence shows that endogenous pyridine nucleotides serve as hydrogen donors for the reaction. The proton translocating function of the redox system ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269886
更新日期:1985-12-01 00:00:00
abstract::Emission and plant uptake of atmospheric nitrogen oxides (NO + NO(2)) significantly influence regional climate change by regulating the oxidative chemistry of the lower atmosphere, species composition and the recycling of carbon and nutrients, etc. Plant uptake of nitrogen dioxide (NO(2)) is concentration-dependent an...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-010-0898-5
更新日期:2010-10-01 00:00:00
abstract::A collection of 29 pathogenesis-related 10 (PR10) genes of Medicago sativa and Medicago truncatula showed that they were almost all obtained from cDNA libraries of tissues undergoing abiotic or biotic stresses. The predicted proteins could be divided into two subclasses, PR10.1 and PR10.2, but in silico predicted mode...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0909-6
更新日期:2010-11-01 00:00:00
abstract::Cell suspension cultures of cowpea (Vigna sp.) were able to produce extracellular peroxidase. Different growth regulator concentrations induced different peroxidase activity in callus. The crude extracellular medium after four weeks of culture showed higher (6 times) specific peroxidase activity and higher thermo stab...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232092
更新日期:1990-07-01 00:00:00
abstract:KEY MESSAGE:The recovery of non-functional-enhanced green fluorescence protein can be used as indicator to facilitate the identification of mutants generated by CRISPR/Cas9. The CRISPR/Cas9 system is a powerful tool for genome editing and it has been employed to knock out genes of interest in multiple plant species. Id...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-019-02465-3
更新日期:2019-12-01 00:00:00
abstract::Genistein, 4',5,7-trihydroxyisoflavone, is an isoflavonoid compound predominantly restricted to legumes and known to possess phyto-oestrogenic and antioxidative activities. The key enzyme that redirects phenylpropanoid pathway intermediates from flavonoids to isoflavonoids is the isoflavone synthase (IFS). Brassica na...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1052-8
更新日期:2011-08-01 00:00:00
abstract::A cDNA clone encoding an ADP-ribosylation factor from potato (Solanum tuberosum L.) was isolated. The nucleotide and deduced amino acid sequences show high homology to known ADP-ribosylation factor sequences from Arabidopsis, yeast, cow and man. In northern blot experiments, all tissues analysed showed expression of t...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233786
更新日期:1994-12-01 00:00:00
abstract::The variegated Saintpaulia cultivar Thamires (Saintpaulia sp.), which has pink petals with blue splotches, is generally maintained by leaf cuttings. In contrast, tissue culture-derived progeny of the cultivar showed not only a high percentage of mutants with solid-blue petals but also other solid-color variants, which...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1016-z
更新日期:2011-05-01 00:00:00
abstract:KEY MESSAGE:Sustainability and safety aspects of plant cell cultures as food are presented. Applicability of dairy side streams as carbon source and use of natural growth enhancers in cultivation are shown. Biotechnologically produced cellular products are currently emerging to replace and add into the portfolio of agr...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-020-02592-2
更新日期:2020-12-01 00:00:00
abstract:KEY MESSAGE:This study established an efficient method of regenerating plants of Ficus lyrata and producing purple-leaved F. lyrata plants through genetic transformation using a VvMybA1 gene of grapevine. ABSTRACT:Ficus lyrata, a species with unique violin- or guitar-shaped leaves, was regenerated from leaf-derived ca...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-013-1491-5
更新日期:2013-11-01 00:00:00
abstract:KEY MESSAGE:A novel rice constitutive promoter (P OsCon1 ) was isolated. The molecular mechanism of the promoter activity was investigated. P OsCon1 could be used as an alternative constitutive promoter for crop transgenic engineering. Monocot constitutive promoter is an important resource for crop transgenic engineeri...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1644-1
更新日期:2014-10-01 00:00:00
abstract::Eukaryotic gene expression is regulated at least by two processes, RNA interference at the post-transcriptional level and chromatin modification at the transcriptional level. Distinct small RNAs (approximately 21-24 nucleotides; sRNAs) were demonstrated to play vital roles in facilitating gene silencing. In plants, th...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1046-6
更新日期:2011-07-01 00:00:00
abstract::The transit peptide sequence of ibAGP2 (TP2) was found to be capable of targeting protein into the chloroplast in the Arabidopsis protoplasts. TP2 was fused to a beta-glucuronidase (GUS) reporter gene and expressed in Arabidopsis under the control of the ibAGP2 promoter with the aim of dissecting the effect of the tra...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0563-4
更新日期:2008-08-01 00:00:00
abstract:KEY MESSAGE:We describe the potato CDPK family and place StCDPK7 as a player in potato response to Phytophthora infestans infection, identifying phenylalanine ammonia lyase as its specific phosphorylation target in vitro. Calcium-dependent protein kinases (CDPKs) decode calcium (Ca2+) signals and activate different sig...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-017-2144-x
更新日期:2017-07-01 00:00:00
abstract::Cotyledon explants from zygotic embryos of Panax ginseng produced somatic embryos on Murashige and Skoog basal medium without growth regulators. Somatic embryos developed directly from epidermal cells at the cotyledon base. Somatic embryos were always formed from the side of the cotyledon opposite to the one attached ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050331
更新日期:1997-10-01 00:00:00
abstract::The expression and functionality of a resveratrol synthase (RS) gene from peanut (Arachis hypogaea) was studied using an Agrobacterium tumefaciens-mediated transient expression system in Nicotiana benthamiana leaves. Functional analysis of RS was demonstrated by tracking its expression during 96 h. To measure the tran...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0664-0
更新日期:2009-04-01 00:00:00
abstract::Extracts of cranberry, rich in flavonols and proanthocyanins, inactivated β-glucuronidase (GUS) in assays using either purified bacterial GUS or preparations of transgenic tobacco (Nicotiana tabacum L.) or transgenic cranberry (Vaccinium macrocarpon Ait.) expressing the gusA gene. Histochemical GUS assays produced ran...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050294
更新日期:1997-06-01 00:00:00
abstract::Programmed cell death (PCD) is a genetically encoded, active process that results in the death of individual cells, tissues, or whole organs, which plays an important role in the life cycles of plants and animals. Previous studies show that methyl salicylate (MeSA) is a defense signal molecular associated with systemi...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1031-0
更新日期:2011-07-01 00:00:00
abstract::Previous work has shown that in rice suspension cells, NaCl at 0.4 M can induce Em gene expression and act synergistically with ABA, possibly by potentiating the ABA response pathway through a rate-limiting intermediate (R.M. Bostock and R.S. Quatrano (1992) Plant Physiol., 98, 1356-1363). Since calcium is an intermed...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232374
更新日期:1996-01-01 00:00:00
abstract:KEY MESSAGE:Phosphate starvation increased the production of phenolic acids by inducing the key enzyme genes in a positive feedback pathway in Saliva miltiorrhiza hairy roots. SPX may be involved in this process. Salvia miltiorrhiza is a wildly popular traditional Chinese medicine used for the treatment of coronary hea...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-016-2007-x
更新日期:2016-09-01 00:00:00
abstract::The in vivo nitrate reductase activity in 8 day old dark-grown sugarcane callus was over three fold that of the light-grown callus. NADH (0.3 mM) in the reaction system, increased the in vivo nitrate reductase activity by more than two fold both in the dark- and the light-grown callus tissues. The NADH dependence of n...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00270207
更新日期:1984-08-01 00:00:00
abstract::TransgenicRhododendron plants were obtained byAgrobacterium tumefaciens-mediated gene transfer.A. tumefaciens harboring a binary vector that contained the chimeric neomycin phosphotransferase II (NPTII) and (3-glucuronidase (GUS) genes was co-cultivated with stem and leaf segments fromRhododendron tissues culturedin v...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01275445
更新日期:1996-11-01 00:00:00
abstract::Efficient protoplast culture and plant regeneration of five U.S. rice cultivars (Oryza sativa L.) - Mercury, Lacassine, Maybelle, Cypress, and Lemont - were obtained from suspension cells maintained in modified General Medium. Embryogenic suspension cells were developed from calli grown on the original callus inductio...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01690249
更新日期:1995-01-01 00:00:00
abstract::Expiants of five plant species (Allium cepa, Antirrhinum majus, Brassica campestris. Glycine max, and Nicotiana tabacum) were co-cultivated with three Agrobacterium tumefaciens strains under different conditions to assess the effects of acetosyringone and medium pH on strain virulence. Tumours were incited on all dico...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00235354
更新日期:1991-04-01 00:00:00
abstract::An efficient culture system has been developed for repeated cycles of somatic embryogenesis in microspore-derived embryos of Brassica juncea without a callus phase. Haploid embryos produced through anther culture showed a high propensity for direct production of somatic embryos in response to 2 mgL(-1) BA and 0.1 mgL(...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00237138
更新日期:1993-03-01 00:00:00
abstract::Surface-sterilized leaf disks of horse-radish (Armoracia lapathifolia) were immersed in a suspension of Agrobacterium rhizogenes harboring the root-inducing plasmid (pRi) and cultured on a solid medium. Within about 10 days after inoculation, adventitious roots (hairy roots) emerged from the leaf disks. No roots emerg...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00271999
更新日期:1987-07-01 00:00:00
abstract::Compact, friable and embryogenic calli were initiated from immature inflorescences and young leaf bases of one week old seedlings of Paspalum scrobiculatum cultured on MS medium supplemented with 2,4-D. A stable, embryogenic suspension culture was initiated from these calli and maintained in a liquid version of the sa...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00274134
更新日期:1989-05-01 00:00:00
abstract::We developed an efficient procedure for transformation and regeneration of L. esculentum cv. Moneymaker from cotyledon explants. The effect of two parameters on the transformation frequency was investigated in detail. The use of feeder layers during cocultivation proved to be critical. In addition, it was found that A...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232816
更新日期:1993-09-01 00:00:00
abstract::The introgression of new desirable characters is very necessary in common bean (Phaseolus vulgaris L.)Interspecific hybridization of P. vulgaris L. with P. angustissimus A. Gray, a wild species with narrow leaves, could be successfully achieved for the first time using embryo culture. Sixteen to twenty-three-day old e...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00268593
更新日期:1986-10-01 00:00:00