Agrobacterium-mediated transformation of Solanum tuberosum L. cv. 'Russet Burbank'.

Abstract:

:Stem sections from shoot cultures maintained in vitro were used to produce transgenic plants of the potato, Solanum tuberosum L. cv. 'Russet Burbank'. Stem internode pieces inoculated with Agrobacterium tumefaciens containing coat protein genes from potato virus X and potato virus Y, produced shoots with a frequency of 60% in the absence of selection and 10% on medium containing 100 mg/l kanamycin monosulfate. Regenerated shoots were assayed for kanamycin resistance by placing stem segments on callus induction medium containing an increased level of kanamycin. Of a total 255 regenerated shoots, 47 (18%) were kanamycin resistant. Of the kanamycin resistant shoots, 25 (53%) expressed the PVX or PVY coat protein genes as assayed by enzyme-linked immunosorbent assay or Western immunoblot analysis.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Newell CA,Rozman R,Hinchee MA,Lawson EC,Haley L,Sanders P,Kaniewski W,Tumer NE,Horsch RB,Fraley RT

doi

10.1007/BF00233028

subject

Has Abstract

pub_date

1991-05-01 00:00:00

pages

30-4

issue

1

eissn

0721-7714

issn

1432-203X

journal_volume

10

pub_type

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