Abstract:
:Stem sections from shoot cultures maintained in vitro were used to produce transgenic plants of the potato, Solanum tuberosum L. cv. 'Russet Burbank'. Stem internode pieces inoculated with Agrobacterium tumefaciens containing coat protein genes from potato virus X and potato virus Y, produced shoots with a frequency of 60% in the absence of selection and 10% on medium containing 100 mg/l kanamycin monosulfate. Regenerated shoots were assayed for kanamycin resistance by placing stem segments on callus induction medium containing an increased level of kanamycin. Of a total 255 regenerated shoots, 47 (18%) were kanamycin resistant. Of the kanamycin resistant shoots, 25 (53%) expressed the PVX or PVY coat protein genes as assayed by enzyme-linked immunosorbent assay or Western immunoblot analysis.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Newell CA,Rozman R,Hinchee MA,Lawson EC,Haley L,Sanders P,Kaniewski W,Tumer NE,Horsch RB,Fraley RTdoi
10.1007/BF00233028subject
Has Abstractpub_date
1991-05-01 00:00:00pages
30-4issue
1eissn
0721-7714issn
1432-203Xjournal_volume
10pub_type
杂志文章abstract::The study used Actinidia deliciosa endosperm-derived callus to investigate aspects of the morphology, histology and chemistry of extracellular matrix (ECM) structures in morphogenically stable tissue from long-term culture. SEM showed ECM as a membranous layer or reticulated fibrillar and granular structure linking th...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0534-9
更新日期:2008-07-01 00:00:00
abstract::Cultured cells of tobacco (Nicotiana tabacum L. cv Petit Havana) were used to investigate signals regulating the expression of the "model" nuclear gene encoding the alternative oxidase (AOX) (AOX1), the terminal oxidase of the mitochondrial alternative respiratory pathway. Several conditions shown to induce AOX1 mRNA ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-004-0848-1
更新日期:2004-12-01 00:00:00
abstract::The Golgi apparatus in plants is organized as a multitude of individual stacks that are motile in the cytoplasm and in close association with the endoplasmic reticulum (ER) (Boevink et al. in Plant J 15:441-447, 1998). These stacks operate as a sorting centre for cargo molecules, providing modification and redirection...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-007-0390-z
更新日期:2007-09-01 00:00:00
abstract::We developed procedures for slow-growth storage of Cedrus atlantica and Cedrus libani microcuttings of juvenile and adult origin, noting factors favouring the extension of subculture intervals. Microcuttings could be stored effectively up to 6 months at 4 degrees C and reduced light intensity, provided that they were ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0129-2
更新日期:2006-07-01 00:00:00
abstract::Diuraphis noxia (Russian wheat aphid, RWA) is a major pest on wheat in South Africa and most other wheat growing countries. Being a probing-sucking insect, RWAs insert their stylets into the phloem sieve elements and feed on the phloem sap. This feeding causes necrotic lesions in resistant varieties, or decoloration o...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0001-9
更新日期:2006-02-01 00:00:00
abstract::Highly viable protoplasts were isolated in large numbers from in vitro-grown leaf and stem tissues of a haploid clone of the apple scion cultivar Golden Delicious (Malus Xdomestica Borkh.). Protoplasts from both sources divided rapidly to give microcallus, when cultured in a modified Kao and Michayluk-based medium. Fo...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00241946
更新日期:1993-01-01 00:00:00
abstract::Grapefruit (Citrus paradisi) tissue cultures were examined for qualitative and quantitative changes in flavanone-neohesperidoside content during somatic embryogenesis. Embryos cultured in vitro contain naringin and a rhamnosyl-transferase activity which is capable of rhamnosylating position 2 on the flavanone glucosid...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00270076
更新日期:1989-10-01 00:00:00
abstract::Plants were regenerated from leafy spurge (Euphorbia esula L.) cell suspensions obtained from stem callus. A North Dakota accession was highly regenerable, but two accessions from Oregon and Austria formed only a few plantlets. Organogenesis occurred in media without growth regulators, under fluorescent lights (30 to ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272536
更新日期:1988-06-01 00:00:00
abstract::Spermidine and ornithine given to normal auxin-requiring cell suspensions of sugarbeet inhibited peroxidase secretion in the absence of Ca(2+). Habituated (organogenic or not) cells did not respond. Both compounds counteracted the Ca(2+) - promoted enzyme secretion by three cell lines. Auxins (2,4-D and BSAA) did not ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00571295
更新日期:1985-05-01 00:00:00
abstract:UNLABELLED:The quality and productivity of chrysanthemum are severely compromised by various abiotic stresses. Here, we describe the isolation of CdICE1 from Chrysanthemum dichrum using RACE PCR, which shared identical nucleotide of ICE1 ORF from Chrysanthemum grandiflorum variety 'Jinba'. CdICE1 contains a conserved b...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-012-1288-y
更新日期:2012-09-01 00:00:00
abstract:KEY MESSAGE:SlHDA3 functions as an inhibitor and regulates tomato fruit ripening and carotenoid accumulation. Post-translational modifications, including histones acetylation, play a pivotal role in the changes of chromatin structure dynamic modulation and gene activity. The regulation of histone acetylation is achieve...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-017-2211-3
更新日期:2018-01-01 00:00:00
abstract:: St. John's wort (Hypericum perforatum. cv 'Anthos') is a medicinal plant with evidence of efficacy as an anti-depressant. The present report describes the development of an in vitro regeneration system that utilizes thidiazuron [N-phenyl-N'-(1,2,3-thidiazol-yl)urea] for the induction of de novo shoots on etiolated hy...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050776
更新日期:2000-05-01 00:00:00
abstract::Mesophyll derived protoplasts of Solanum khasianum Clarke and S. aculeatissimum Jacq. were fused by electrofusion and resulted in interspecific hybrids. In total, 249 calluses were obtained from which more than 65% produced shoots. From the regenerated plantlets 45% could be identified as hybrids by chromosome counts ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00239891
更新日期:1994-01-01 00:00:00
abstract::Two clones of Populus nigra L. were tested in vivo and in vitro for their susceptibility to three different Agrobacterium tumefaciens wild-type strains evaluating number and size of resulting calluses. Strain C58 proved to be the most virulent.Various parameters affecting Agrobacterium-mediated transformation of P. ni...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233315
更新日期:1994-02-01 00:00:00
abstract::Salt tolerant cell lines have been selected from Medicago sativa, by a single step selection process on tissue culture medium containing 1% NaCl. Plants regenerated from these lines show improved salt tolerance compared to parent plants. The regenerated plants are vigorous, have flowered and are self fertile. The cell...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232511
更新日期:1991-12-01 00:00:00
abstract::The nuclear DNA content of sweet pepper (Capsicum annuum L. var. annuum, 2n = 24) has been measured by flow and image cytometries but the DNA content of each chromosome of this species has not yet been regarded. DNA content of individual chromosomes has been quantified by the flow karyotyping technique, which requires...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0539-4
更新日期:2008-07-01 00:00:00
abstract::Programmed cell death (PCD) is a process that occurs both in animals and in plants and is an essential element in developmental processes. Pollination is a key factor in fruit production and self-incompatibility is one of the main limiting factors of this process. PCD has recently been put forward as a possible cause ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0845-5
更新日期:2010-06-01 00:00:00
abstract::The effects of serial combinations of either indole-3-acetic acid, indole-3-butyric acid or α-naphthaleneacetic acid (0.5-10.0 mg/l) with either kinetin, 6-benzyl-amino-purine, zeatin or 6-methylaminopurine (0.5-5.0 mg/l) have been investigated to assess the morphogenetic potential of foliar explants of Duboisia myopo...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269711
更新日期:1986-02-01 00:00:00
abstract::Green fluorescent protein (GFP) has been used widely as a powerful bioluminescent reporter, but its visualization by existing methods in tissues or whole plants and its utilization for high-throughput screening remains challenging in many species. Here, we report a fluorescence image analyzer-based method for GFP dete...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0657-z
更新日期:2009-03-01 00:00:00
abstract::Leaf explants ofKalanchoe laciniata were cocultivated for different days (2, 4, 6 and 8 days) with disarmedAgrobacterium tumefaciens strains A208SE, GV3111SE and EHA101 carring a binary vector pROA93. The vector contains a cauliflower mosaic virus 35S promotor which drives the coding sequence of neomycin phosphotransf...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00716668
更新日期:1989-06-01 00:00:00
abstract:: Cells of a suspension culture of Doritaenopsis cv. New Toyohashi were placed in a mixture of 2 M glycerol and 0.4 M sucrose for 15 min at room temperature and then dehydrated with a vitrification solution (PVS2) for 1-3 h on ice and plunged into liquid nitrogen. The highest viability (64% by 2,3,5-triphenyltetrazoliu...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000255
更新日期:2000-12-01 00:00:00
abstract::TransgenicRhododendron plants were obtained byAgrobacterium tumefaciens-mediated gene transfer.A. tumefaciens harboring a binary vector that contained the chimeric neomycin phosphotransferase II (NPTII) and (3-glucuronidase (GUS) genes was co-cultivated with stem and leaf segments fromRhododendron tissues culturedin v...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01275445
更新日期:1996-11-01 00:00:00
abstract::An efficient plant regeneration system from leaf-derived callus in 6 genotypes of Avena sativa L. has been established. Regenerable callus was induced in the basal 1-2 mm region of 2 to 5 day old seedlings. Plants were regenerated from the regenerable callus and grown to maturity. The frequency of regenerable callus f...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00238604
更新日期:1995-03-01 00:00:00
abstract::Low initiation frequency is one of the main barriers in applying somatic embryogenesis to the clonal production of Pinus species. Factors affecting initiation, including basal medium, plant growth regulators, and Phytagel concentration, have been investigated in loblolly pine (Pinus taeda L.). BM1 basal medium proved ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050396
更新日期:1998-02-01 00:00:00
abstract:KEY MESSAGE:Overexpression of SoSnRK2.1 improved drought tolerance and growth of tobacco plants. Sucrose non-fermenting1-related protein kinase 2 (SnRK2) is a key enzyme in regulating ABA signal transduction in plants, and it plays a significant role in response to multiple abiotic stresses. In this research, SoSnRK2.1...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-016-2004-0
更新日期:2016-09-01 00:00:00
abstract::Chimeric constructs consisting of the gus coding region fused downstream of promoterun-translated leader sequences from the tobacco osmotin and PR-S genes, the potato proteinase inhibitor 2 gene (pin2), and the cauliflower mosaic virus (CaMV) 35S promoter were biolistically transferred into sugarbeet suspension cells....
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233152
更新日期:1996-08-01 00:00:00
abstract::Shoot buds of ginger were successfully encapsulated in 4% sodium alginate gel. Encapsulated buds were germinated in vitro to form roots and shoots. In vitro germination (emergence of sprouts) of encapsulated buds ranged from 16.7% to 81.8% on different media after 5 weeks of incubation. Normal plantlets with an averag...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233325
更新日期:1994-02-01 00:00:00
abstract:: Somatic embryogenesis and whole plant regeneration were achieved in callus cultures derived from immature zygotic embryos of Acacia mangium. Embryogenic callus was induced on MS medium containing combinations of TDZ (1-2 mg/l), IAA (0.25-2 mg/l) and a mixture of amino acids. Globular embryos developed on embryogenic ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000288
更新日期:2001-01-01 00:00:00
abstract:KEY MESSAGE:The Arabidopsis AP2/ERF family transcription factor AtERF15 is nuclear-localized and positively regulates ABA and stress responses. Abscisic acid (ABA) is a major plant hormone that controls the expression of hundreds genes involved in various aspects of plant growth and development, such as seed developmen...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1688-2
更新日期:2015-01-01 00:00:00
abstract::We developed an efficient procedure for transformation and regeneration of L. esculentum cv. Moneymaker from cotyledon explants. The effect of two parameters on the transformation frequency was investigated in detail. The use of feeder layers during cocultivation proved to be critical. In addition, it was found that A...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232816
更新日期:1993-09-01 00:00:00