Abstract:
KEY MESSAGE:SlHDA3 functions as an inhibitor and regulates tomato fruit ripening and carotenoid accumulation. Post-translational modifications, including histones acetylation, play a pivotal role in the changes of chromatin structure dynamic modulation and gene activity. The regulation of histone acetylation is achieved by the action of histone acetyltransferases and deacetylases, which play crucial roles in the regulation of transcription activation. There is an increasing research focus on histone deacetylation in crops, but the role of histone deacetylase genes (HDACs) in tomato has not been elucidated. With the aim of characterizing the tomato RPD3/HDA1 family histone deacetylase genes, SlHDA3 was isolated and its RNA interference (RNAi) lines was obtained. The fruit of SlHDA3 RNAi lines exhibited accelerated ripening process along with short shelf life characteristics. The accumulation of carotenoid was increased due to the alteration of the carotenoid pathway flux. Climacteric ethylene production also stimulated along with significantly up-regulated expression of ethylene biosynthetic genes (ACS2, ACS4, ACO1 and ACO3) and fruit ripening-associated genes (RIN, E4, E8, PG, Pti4, LOXB, Cnr and TAGL1) in SlHDA3 RNAi lines. Besides, fruit cell wall metabolism-associated genes (HEX, MAN, TBG4, XTH5 and XYL) were enhanced in transgenic lines. Relative to wild type (WT) plants, SlHDA3 RNAi seedlings displayed shorter hypocotyls and more sensitivity to ACC (1-aminocyclopropane-1-carboxylate). These results indicated that SlHDA3 is involved in the regulation of fruit ripening by affecting ethylene biosynthesis and carotenoid accumulation.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Guo JE,Hu Z,Yu X,Li A,Li F,Wang Y,Tian S,Chen Gdoi
10.1007/s00299-017-2211-3subject
Has Abstractpub_date
2018-01-01 00:00:00pages
125-135issue
1eissn
0721-7714issn
1432-203Xpii
10.1007/s00299-017-2211-3journal_volume
37pub_type
杂志文章abstract::The growth of Distichlis spicata suspension cultures in LS medium without NaCl was inhibited 54% by 2 mM proline. In medium containing 260 mM NaCl, 10 mM proline inhibited growth by only 22%. The uptake and metabolism of 10 mM L-[1-(13)C] proline was followed by (13)C NMR and ninhydrin analyses of suspensions cultured...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269924
更新日期:1988-08-01 00:00:00
abstract::Cell suspension cultures of bean (Phaseolus vulgaris) cv. Imuna accumulated isoflavonoid phytoalexins on exposure to elicitor from the phytopathogenic fungus Colletotrichum lindemuthianum (CL). This was preceeded by rapid increases in the activities of phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS). How...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269060
更新日期:1989-12-01 00:00:00
abstract::An efficient culture system has been developed for repeated cycles of somatic embryogenesis in microspore-derived embryos of Brassica juncea without a callus phase. Haploid embryos produced through anther culture showed a high propensity for direct production of somatic embryos in response to 2 mgL(-1) BA and 0.1 mgL(...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00237138
更新日期:1993-03-01 00:00:00
abstract::Protoplasts isolated enzymatically from seedling roots, hypocotyls and cotyledons of Lotus corniculatus L. produced callus which underwent prolific shoot regeneration. The rapidity and ease of recovering plants from protoplast-derived tissues makes this forage legume an attractive experimental system for genetic manip...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00270177
更新日期:1983-04-01 00:00:00
abstract::Most perennial plants must pass through a long juvenile phase of vegetative development before they are capable of flowering. We have developed a method specifying inflorescence proliferation to bypass juvenility and maintain the adult phase. Bamboo ( Bambusa edulis) inflorescences were amplified by incubation in Mura...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0571-3
更新日期:2003-06-01 00:00:00
abstract:UNLABELLED:Calmodulin-binding proteins (CBPs) have been known to be involved in both biotic and abiotic stress responses. Recently, two closely related CBPs, Arabidopsis SAR Deficient 1 and CBP60g, were found to belong to a new family of transcription factors that regulate salicylic acid (SA) biosynthesis triggered by ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-012-1247-7
更新日期:2012-07-01 00:00:00
abstract::Stable cell suspension cultures have been established from immature endosperms of A69Y wild-type and opaque-2 maize (Zea mays L.). Cultured cells are capable of storage protein (zein) synthesis and accumulation throughout the growth period. Electrophoretic patterns of zeins show, for opaque-2 cells, the preferential i...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232330
更新日期:1991-02-01 00:00:00
abstract:KEY MESSAGE:MicroRNAs have higher expression stability than protein-coding genes in B. napus seeds and are therefore good reference genes for miRNA and mRNA RT-qPCR analysis. Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) has become the "gold standard" to gain insight into function of ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1773-1
更新日期:2015-07-01 00:00:00
abstract::High yields of protoplasts were obtained from leaves of aseptically grown plants and calli originated from different explants, in several cultivars of Cajanus cajan L. The protoplasts divided to form cell clusters in modified KM 8p medium and developed to protocolonies after dilution with liquid Caboche's medium withi...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232691
更新日期:1992-08-01 00:00:00
abstract::Accurate and timely detection of transgene copy number in sugarcane is currently hampered by the requirement to use Southern blotting, needing relatively large amounts of genomic DNA and, therefore, the continued growth and maintenance of bulky plants in containment glasshouses. In addition, the sugarcane genome is bo...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1150-7
更新日期:2012-01-01 00:00:00
abstract::A method to obtain plants from embryogenic callus of Brassica nigra and protoplasts of hypocotyl expiants is described. Callus was initiated on Murashige and Skoog medium containing kinetin (kn) and 2,4-dichlorophenoxy acetic acid (2,4-D). Lowering of auxin induced embryo formation. Supplementation with gibberellic ac...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232265
更新日期:1990-12-01 00:00:00
abstract::An early identification of fusion products was based on the presumed vigorous growth of hybrid calluses after fusion between Solanum brevidens and S. tuberosum leaf protoplasts. The S. brevidens protoplasts were unable to form multicellular colonies under the applied culture conditions. Three size groups of calluses w...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00234700
更新日期:1993-05-01 00:00:00
abstract::Fully fertile plants with the expected chromosome number 2n=40 were regenerated from excised leaf sections of Glycine clandestina. Shoots formed directly on the explants through organogenesis. Utilizing the same media and procedures fully fertile plants were also regenerated from cotyledon and hypocotyl tissue of the ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269116
更新日期:1986-06-01 00:00:00
abstract::Arabinogalactan proteins (AGPs) are important proteoglycans regulating somatic embryogenesis in diverse plant species. Embryogenic cells of somatic embryos are covered by special extracellular cell wall layer called extracellular surface matrix network (ECMSN) at their early developmental stages. Here we show that hig...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-007-0429-1
更新日期:2008-02-01 00:00:00
abstract::Five varieties of durum wheat: Appulo, Ofanto, Latino, Creso, and Castello (Triticum durum Desf.) adapted to the semi-arid mediterranean environment have been tested for their in vitro response. Compact, embryogenic, highly regenerable calli originated from primary callus derived through proliferation of the scutellum...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00193145
更新日期:1991-09-01 00:00:00
abstract:KEY MESSAGE:FLA, the homolog of ubiquitin-specific protease does not have deubiquitination activity, but it is essential for flower and chloroplast development in rice. Ubiquitin-specific proteases (UBPs) are widely distributed and highly conserved proteins and are also members of the most important family of deubiquit...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-018-2368-4
更新日期:2019-03-01 00:00:00
abstract:KEY MESSAGE:We provide evidence that nucleotide sequence and methylation status changes occur in the Arabidopsis genome during in vitro tissue culture at a frequency high enough to represent an important source of variation. Somaclonal variation is a general consequence of the tissue culture process that has to be anal...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-017-2217-x
更新日期:2018-01-01 00:00:00
abstract::Glyoxalase-I activity in growingDatura callus showed 184% increase with the age of the culture. Spermidine increased the enzyme activity together with DNA and protein synthesis. With the addition of mitotic inhibitors, vinblastine and methylglyoxal in the growth medium, the enzyme activity was inhibited by 92 percent ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF02441015
更新日期:1984-06-01 00:00:00
abstract::Genetic transformation of arctic bramble (Rubus arcticus L.) was achieved utilizing a Ti-plasmid vector system of Agrobacterium tumefaciens. Internodal stem segments were inoculated with Agrobacterium strain EHA101 carrying a T-DNA with the CaMV 35 S promoter-gus-int marker gene from which β-glucuronidase (GUS) is exp...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050491
更新日期:1998-07-01 00:00:00
abstract::A protocol for protoplast isolation was developed and tested with five Capsicum genotypes representing two cultivated species, C. annuum and C. chinense. Key variables included growth conditions for source plants and the concentration of mannitol used as osmoticum. Protoplasts isolated from each of the genotypes becam...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00234146
更新日期:1994-04-01 00:00:00
abstract::The Golgi apparatus in plants is organized as a multitude of individual stacks that are motile in the cytoplasm and in close association with the endoplasmic reticulum (ER) (Boevink et al. in Plant J 15:441-447, 1998). These stacks operate as a sorting centre for cargo molecules, providing modification and redirection...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-007-0390-z
更新日期:2007-09-01 00:00:00
abstract::Polyclonal antibodies against a part of pea (Pisum sativum L.) LOXG protein have been raised to study the pattern of distribution of related lipoxygenases in pea carpels. The antiserum recognized three lipoxygenase polypeptides in carpels. One of them became undetectable 24 hours after fruit development induction, sug...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232465
更新日期:1996-04-01 00:00:00
abstract::We developed procedures for slow-growth storage of Cedrus atlantica and Cedrus libani microcuttings of juvenile and adult origin, noting factors favouring the extension of subculture intervals. Microcuttings could be stored effectively up to 6 months at 4 degrees C and reduced light intensity, provided that they were ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0129-2
更新日期:2006-07-01 00:00:00
abstract:: An Agrobacterium tumefaciens-mediated transformation method has been developed for onions (Allium cepa L.) using immature embryos as the explant source. Transgenic plants were recovered from the open-pollinated onion cultivar Canterbury Longkeeper at a maximum transformation frequency from immature embryos of 2.7%. T...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050743
更新日期:2000-03-01 00:00:00
abstract::The goal of the study was to characterize the optimal developmental stage of zygotic embryo expiants of the hardwood forest tree species yellow-poplar (Liriodendron tulipifera L.) for the initiation of embryogenic cultures, using morphological measurements and polypeptide profiles of the embryos. Developing zygotic em...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00234289
更新日期:1991-07-01 00:00:00
abstract::Methods of shoot and plant development from cotyledon explants of the tomato cultivar 'Bonner Beste' and its mutant chloronerva are described. Cotyledon mesophyll protoplasts of the mutant chloronerva have been isolated and cultured, and induced to regenerate shoots and plants. By grafting regenerated shoots of the ch...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00270102
更新日期:1983-08-01 00:00:00
abstract:KEY MESSAGE:A plant-derived 0.3 kb constitutive promoter was obtained from AtTCTP expression analysis, and successfully applied to the expression of a selectable marker gene for production of transgenic creeping bentgrass plants. The isolation and use of an efficient promoter is essential to develop a vector system for...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1705-5
更新日期:2015-02-01 00:00:00
abstract:KEY MESSAGE:We describe the potato CDPK family and place StCDPK7 as a player in potato response to Phytophthora infestans infection, identifying phenylalanine ammonia lyase as its specific phosphorylation target in vitro. Calcium-dependent protein kinases (CDPKs) decode calcium (Ca2+) signals and activate different sig...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-017-2144-x
更新日期:2017-07-01 00:00:00
abstract::Leaves excised from shoot cultures of Prunus avium cvs. F12/1 and Charger and genotype 1908, and from five genotypes of P. serotina and two hybrids of P. avium×P. sargentii developed shoots on Woody Plant medium (WPM) supplemented with either benzyladenine (BA) or thidiazuron (TDZ). Regeneration in both P. avium 1908 ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050436
更新日期:1998-04-01 00:00:00
abstract::Several lipid classes constitute the universal matrix of the biological membranes. With their amphipathic nature, lipids not only build the continuous barrier that confers identity to every cell and organelle, but they are also active actors that modulate the activity of the proteins immersed in the lipid bilayer. The...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-014-1735-z
更新日期:2015-04-01 00:00:00