Transformation of arctic bramble (Rubus arcticus L.) by Agrobacterium tumefaciens.

Abstract:

:Genetic transformation of arctic bramble (Rubus arcticus L.) was achieved utilizing a Ti-plasmid vector system of Agrobacterium tumefaciens. Internodal stem segments were inoculated with Agrobacterium strain EHA101 carrying a T-DNA with the CaMV 35 S promoter-gus-int marker gene from which β-glucuronidase (GUS) is expressed only in plants. Regenerants were produced on Murashige and Skoog medium. Growth of Agrobacterium was inhibited with cefotaxime. Kanamycin was used as the selective agent for the transformants. Regenerants were assayed by histochemical GUS staining, and by Southern analysis using a gus-int probe. Transgenic arctic bramble plants containing gus-int and expressing GUS were recovered. Expression has been stable for 3 years in micropropagation.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Kokko HI,Kärenlampi SO

doi

10.1007/s002990050491

subject

Has Abstract

pub_date

1998-07-01 00:00:00

pages

822-826

issue

10

eissn

0721-7714

issn

1432-203X

pii

10.1007/s002990050491

journal_volume

17

pub_type

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