Abstract:
:Protocols were developed for regeneration and Agrobacterium-mediated transformation of Actinidia eriantha Benth. A. eriantha has a number of features that make it a useful tool for functional genomics in Actinidia: the vines are relatively small and non-vigorous in nature, flowers form all over the vine including on lower axillary branches and the species flowers prolifically in greenhouse conditions. Flowering and fruiting of transgenic A. eriantha plants was obtained within 2 years of transformation in a containment greenhouse. GUS (beta-glucuronidase) activity indicating stable expression of the uidA gene was observed in leaf, stem, root, petal and fruit tissues. Molecular evidence for incorporation of transgenes into the A. eriantha genome was obtained by PCR and DNA gel blot analysis. Inheritance of transgenic phenotypes was demonstrated in seedling progeny. Functional genomic studies in kiwifruit have been initiated using transgenic A. eriantha plants.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Wang T,Ran Y,Atkinson RG,Gleave AP,Cohen Ddoi
10.1007/s00299-005-0080-7keywords:
subject
Has Abstractpub_date
2006-05-01 00:00:00pages
425-31issue
5eissn
0721-7714issn
1432-203Xjournal_volume
25pub_type
杂志文章abstract::Embryogenic callus and suspension cultures of carrot (Daucus carota L., cv. Nantaise), growing on/in medium including 1 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D), were transferred to medium with or without this plant growth regulator, to impair or induce, respectively, further development of somatic embryos. The en...
journal_title:Plant cell reports
pub_type: 杂志文章
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journal_title:Plant cell reports
pub_type: 杂志文章
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272107
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journal_title:Plant cell reports
pub_type: 杂志文章
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00276676
更新日期:1987-04-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050004
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journal_title:Plant cell reports
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doi:10.1007/BF00269896
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00238604
更新日期:1995-03-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
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journal_title:Plant cell reports
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journal_title:Plant cell reports
pub_type: 杂志文章
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00237138
更新日期:1993-03-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0594-x
更新日期:2008-11-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0690-x
更新日期:2003-11-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1761-5
更新日期:2015-06-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0109-y
更新日期:2006-06-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0092-3
更新日期:2006-04-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269202
更新日期:1985-04-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232404
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journal_title:Plant cell reports
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pub_type: 杂志文章
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050520
更新日期:1998-09-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232066
更新日期:1996-02-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-004-0848-1
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0539-4
更新日期:2008-07-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050331
更新日期:1997-10-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0598-5
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00236463
更新日期:1991-06-01 00:00:00
abstract::Cell suspension cultures of tomato (Lycopersicon esculentum) adapted to growing continuously in the presence of 0.1 mM CdCl2 and accumulated phytochelatins (PCs, poly(γ-Glu-Cys)n-Gly). The highest level of PCs was measured 4 days after inoculation and coincided with the peak of cellular cadmium concentration. At this ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232275
更新日期:1990-12-01 00:00:00
abstract::Nineteen accessions of diploid Medicago sativa L. belonging to the four subspecies sativa, caerula, falcata and xvaria were screened for their ability to produce somatic embryos on hypocotyl-derived callus. Two medium protocols were used in this study, a three-step sequence with exposure of the callus cultures to a hi...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269379
更新日期:1985-10-01 00:00:00