Transformation of Actinidia eriantha: a potential species for functional genomics studies in Actinidia.

Abstract:

:Protocols were developed for regeneration and Agrobacterium-mediated transformation of Actinidia eriantha Benth. A. eriantha has a number of features that make it a useful tool for functional genomics in Actinidia: the vines are relatively small and non-vigorous in nature, flowers form all over the vine including on lower axillary branches and the species flowers prolifically in greenhouse conditions. Flowering and fruiting of transgenic A. eriantha plants was obtained within 2 years of transformation in a containment greenhouse. GUS (beta-glucuronidase) activity indicating stable expression of the uidA gene was observed in leaf, stem, root, petal and fruit tissues. Molecular evidence for incorporation of transgenes into the A. eriantha genome was obtained by PCR and DNA gel blot analysis. Inheritance of transgenic phenotypes was demonstrated in seedling progeny. Functional genomic studies in kiwifruit have been initiated using transgenic A. eriantha plants.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Wang T,Ran Y,Atkinson RG,Gleave AP,Cohen D

doi

10.1007/s00299-005-0080-7

keywords:

subject

Has Abstract

pub_date

2006-05-01 00:00:00

pages

425-31

issue

5

eissn

0721-7714

issn

1432-203X

journal_volume

25

pub_type

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