Effect of promoter-leader sequences on transient expression of reporter gene chimeras biolistically transferred into sugarbeet (Beta vulgaris) suspension cells.

abstract：:A method for regenerating pigeonpea [Cajanus cajan (L.) Millsp.] plants has been developed using distal cotyledonary segments of mature seeds as explants. A large number of shoot buds were induced directly from explants of genotypes T-15-15 and GAUT-82-90 when cultured on six different basal media fortified with 22.2 ...

journal_title：Plant cell reports

authors： Mohan ML,Krishnamurthy KV

更新日期：1998-06-01 00:00:00

abstract：:Advancement in the field of genetics and genomics after the discovery of Mendel's laws of inheritance has led to map the genes controlling qualitative and quantitative traits in crop plant species. Mapping of genomic regions controlling the variation of quantitatively inherited traits has become routine after the adve...

journal_title：Plant cell reports

authors： Kumar J,Gupta DS,Gupta S,Dubey S,Gupta P,Kumar S

更新日期：2017-08-01 00:00:00

abstract：:Recent developments in modern biotechnology such as the use of RNA interference (RNAi) have broadened the scope of crop genetic modification. RNAi strategies have led to significant achievements in crop protection against biotic and abiotic stresses, modification of plant traits, and yield improvement. As RNAi-derived...

journal_title：Plant cell reports

authors： Darsan Singh JK,Mat Jalaluddin NS,Sanan-Mishra N,Harikrishna JA

更新日期：2019-12-01 00:00:00

abstract：: Rooted regenerated shoots obtained from leaf and nodal segments of Rosmarinus officinalis were grown on a basal nutrient medium for 9 weeks. The regenerants were shown by means of HPLC and mass spectrometry to contain carnosic acid, a diterpenoid with antioxidant and medicinal properties. Five-week-old nodular green ...

journal_title：Plant cell reports

authors： Caruso JL,Callahan J,DeChant C,Jayasimhulu K,Winget GD

更新日期：2000-04-01 00:00:00

abstract：:Medicago sativa lines with a high incidence of regeneration were established as suspension cultures and used to select for NaCl tolerant lines. Attempts were then made to regenerate plants from these lines. Regeneration was severely depressed in NaCl tolerant calli and the only plants that were successfully regenerate...

journal_title：Plant cell reports

authors： Smith MK,McComb JA

更新日期：1983-06-01 00:00:00

abstract：:Somatic embryos and plantlets have been obtained in quite a direct way from mesophyll protoplasts isolated from androgenetic plants of two cultivars ("Loras" and "Tower") of Brassica napus. The procedure consists of four steps. Proembryos were induced in a medium supplemented with a cytokinin and an auxin at comparati...

journal_title：Plant cell reports

authors： Li LC,Kohlenbach HW

更新日期：1982-10-01 00:00:00

abstract：:In vitro culture of pineapple (Ananas comosus) was studied to determine the efficiency of axillary bud culture for rapid propagation of several cultivars. The technique used maximizes the success rate of various steps in the production of pineapple plants. Rapid mass multiplication of plantlets started 9 months after ...

journal_title：Plant cell reports

authors： Dewald MG,Moore GA,Sherman WB,Evans MH

更新日期：1988-12-01 00:00:00

abstract：:Plant meiotic prophase I is a complicated process involving the late zygotene and pachytene stages, both crucial for completing synapsis and recombination. Using David Lily (Lilium davidii var. Willmottiae) as our research material, we performed suppression subtractive hybridization to construct EST library of anthers...

journal_title：Plant cell reports

authors： Mu C,Wang S,Zhang S,Pan J,Chen N,Li X,Wang Z,Liu H

更新日期：2011-10-01 00:00:00

abstract：:An endo-pectate lyase (PL; EC 4.2.2.2), originally cloned fiom the phytopathogenic bacterium Erwinia chrysanthemi EC16, was expressed in recA (-) E. coli strain DK1, purified to a single band by isoelectric focusing and used to induce berberine production in established plant suspension cultures of Thalictrum minus L....

journal_title：Plant cell reports

authors： Smolko DD,Peretti SW

更新日期：1994-12-01 00:00:00

abstract：:Agrobacterium-mediated transformation frequency is very low with cells from some species such as soybeans. Studies were conducted to investigate the Agrobacterium-mediated transformation frequency in near-isogenic lipoxygenase mutant lines of soybeans, since the nigh level of lipoxygenase activity in soybean embryos m...

journal_title：Plant cell reports

authors： Bhatt D,Parrott WA,Collins GB,Hildebrand DF

更新日期：1991-03-01 00:00:00

abstract：:Plants were regenerated from leafy spurge (Euphorbia esula L.) cell suspensions obtained from stem callus. A North Dakota accession was highly regenerable, but two accessions from Oregon and Austria formed only a few plantlets. Organogenesis occurred in media without growth regulators, under fluorescent lights (30 to ...

journal_title：Plant cell reports

authors： Davis DG,Olson PA,Stolzenberg RL

更新日期：1988-06-01 00:00:00

abstract：:The activity and activation potential of polyphenoloxidase (PPO, E.C. 1.10.3.1.) of tissue from shoot tips, adult leaves and embryogenic and non-embryogenic cell suspension cultures of Euphorbia pulcherrima was investigated using an oxygen probe technique. PPO derived from differentiated in vivo plant tissue (shoot ti...

journal_title：Plant cell reports

authors： Grotkass C,Lieberei R,Preil W

更新日期：1995-04-01 00:00:00

abstract：: The influences of light conditions, sucrose and ethylene on in vitro formation and storability of onion (Allium cepa L.) bulblets were studied in various accessions. Light, sucrose and ethylene influenced bulb formation. Storability was primarily enhanced by a high sucrose concentration (100 g/l) in the culture mediu...

journal_title：Plant cell reports

authors： Kästner U,Klahr A,Keller ERJ,Kahane R

更新日期：2001-02-01 00:00:00

abstract：:In an effort to develop a chemically inducible system for insect management, we studied production of Cry1Ab Bacillus thuringiensis (Bt) protein and control of the diamondback moth (DBM), Plutella xylostella L., in inducer-treated and untreated tissues of a broccoli line transformed with a PR-1a/cry1Ab expression cass...

journal_title：Plant cell reports

authors： Cao J,Bates SL,Zhao JZ,Shelton AM,Earle ED

更新日期：2006-06-01 00:00:00

abstract：:A protocol for protoplast isolation was developed and tested with five Capsicum genotypes representing two cultivated species, C. annuum and C. chinense. Key variables included growth conditions for source plants and the concentration of mannitol used as osmoticum. Protoplasts isolated from each of the genotypes becam...

journal_title：Plant cell reports

authors： Murphy JF,Kyle MM

更新日期：1994-04-01 00:00:00

abstract：KEY MESSAGE:A chalcone synthase gene ( PaCHS ) was isolated and functionally characterized from liverwort. The ectopic expression of PaCHS in Marchantia paleacea callus raised the flavonoids content. Chalcone synthase (CHS; EC 2.3.1.74) is pivotal for the biosynthesis of flavonoid and anthocyanin pigments in plants. It...

journal_title：Plant cell reports

authors： Yu HN,Wang L,Sun B,Gao S,Cheng AX,Lou HX

更新日期：2015-02-01 00:00:00

abstract：:In vitro adventitious shoot formation was examined in petioles and laminae ofPaulownia elongata, P. fortunei, andP. 'Henan 1'. Fully expanded, dark green, thick, older leaves were healthier and exhibited greater callus and shoot production than did young leaves. The ten clones used varied greatly in adventitious shoot...

journal_title：Plant cell reports

authors： Bergmann BA,Moon HK

更新日期：1997-02-01 00:00:00

abstract：:The production of cybrids, combining nucleus of a species with alien cytoplasmic organelles, is a valuable method used for improvement of various crops. Several citrus cybrids have been created by somatic hybridization. These genotypes are interesting models to analyze the impact of cytoplasmic genome change on nuclea...

journal_title：Plant cell reports

authors： Bassene JB,Froelicher Y,Navarro L,Ollitrault P,Ancillo G

更新日期：2011-06-01 00:00:00

abstract：KEY MESSAGE:In GhCFE5 homoeologs, GhCFE5D interacted with more actin homologs and stronger interaction activity than GhCFE5A. GhCFE5D - but not GhCFE5A -overexpression severely disrupted actin cytoskeleton organization and significantly suppressed cell elongation. Homoeologous genes are common in polyploid plants; howe...

journal_title：Plant cell reports

authors： Lv F,Li P,Zhang R,Li N,Guo W

更新日期：2016-04-01 00:00:00

abstract：:In a study of the 5'-flanking sequence of the Zea mays L. (maize) Glb1 gene in vitro, serial promoter deletions were generated and linked with the β-glucuronidase (GUS) reporter gene. The promoter deletion-GUS fusions were introduced into the maize P3377 cell line by particle bombardment. GUS assays indicated that tre...

journal_title：Plant cell reports

authors： Liu S,Kriz A,Duncan D,Widholm J

更新日期：1998-05-01 00:00:00

abstract：:The in vivo nitrate reductase activity in 8 day old dark-grown sugarcane callus was over three fold that of the light-grown callus. NADH (0.3 mM) in the reaction system, increased the in vivo nitrate reductase activity by more than two fold both in the dark- and the light-grown callus tissues. The NADH dependence of n...

journal_title：Plant cell reports

authors： Khan BM,Dwivedi UN,Rawal SK,Mascarenhas AF

更新日期：1984-08-01 00:00:00

abstract：:Rosmarinic acid (RA) is a natural antioxidant produced by cell suspension cultures of sage (Salvia officinalis L.). The growth and production of RA by these cells can be modified by the type of culture medium. Production can be increased 10-fold to attain 6.4 g.1(-1) under optimal conditions. Investigation of kinetics...

journal_title：Plant cell reports

authors： Hippolyte I,Marin B,Baccou JC,Jonard R

更新日期：1992-04-01 00:00:00

abstract：KEY MESSAGE:The first Good Manufacturing Practices production of a purification-free rice-based oral cholera vaccine (MucoRice-CTB) from transgenic plants in a closed cultivation system yielded a product meeting regulatory requirements. Despite our knowledge of their advantages, plant-based vaccines remain unavailable ...

journal_title：Plant cell reports

authors： Kashima K,Yuki Y,Mejima M,Kurokawa S,Suzuki Y,Minakawa S,Takeyama N,Fukuyama Y,Azegami T,Tanimoto T,Kuroda M,Tamura M,Gomi Y,Kiyono H

更新日期：2016-03-01 00:00:00

abstract：KEY MESSAGE:MicroRNAs have higher expression stability than protein-coding genes in B. napus seeds and are therefore good reference genes for miRNA and mRNA RT-qPCR analysis. Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) has become the "gold standard" to gain insight into function of ...

journal_title：Plant cell reports

authors： Machado RD,Christoff AP,Loss-Morais G,Margis-Pinheiro M,Margis R,Körbes AP

更新日期：2015-07-01 00:00:00

abstract：KEY MESSAGE:A R2R3-MYB transcription factor EsAN2 was isolated from Epimedium sagittatum and functionally characterized to regulate the anthocyanin biosynthetic pathway. Epimedium plants are used widely both as traditional Chinese medicinal herbs and ornamental perennials. Anthocyanins, acting as major contributors to ...

journal_title：Plant cell reports

authors： Huang W,Khaldun AB,Lv H,Du L,Zhang C,Wang Y

更新日期：2016-04-01 00:00:00

abstract：:Independent carrot (Daucus carota) hairy root lines were established by inoculation of discs taken from the same carrot with Agrobacterium rhizogenes 8196 and A. tumefaciens C58C1(pRi8196) carrying pRi8196. Several lines were compared with respect to T-DNA length. One of them was found to have integrated sequences cov...

journal_title：Plant cell reports

authors： David C,Petit A,Tempé J

更新日期：1988-03-01 00:00:00

abstract：:In the present paper, attempts were made to explore the possibility of employing ultraviolet (UV) irradiation in citrus asymmetric fusion for transfer of limited amount of favorable traits from a desirable cultivar to a target one. Exposure of Satsuma mandarin (Citrus unshiu Marc.) embryogenic protoplasts to UV at an ...

journal_title：Plant cell reports

authors： Xu XY,Hu ZY,Li JF,Liu JH,Deng XX

更新日期：2007-08-01 00:00:00

abstract：:Protoplasts isolated from mesophyll cells of Eruca sativa Lam., cultured on suitable medium, underwent sustained cell divisions to form calli. The plating efficiency was found to be 0.4%. The protoplast-derived calli subsequently produced plantlets through organogenesis (15.71%) and somatic embryogenesis (11.25%). Reg...

journal_title：Plant cell reports

authors： Sikdar SR,Chatterjee G,Das S,Sen SK

更新日期：1987-12-01 00:00:00

abstract：:A new method of estimating the amount of berberine released from minute cell colonies of Thalictrum minus has been devised to facilitate the selection of high berberine-producing cell lines. In this system, cell aggregates obtained from a cell suspension culture are grown on small pieces of an agar culture medium and ...

journal_title：Plant cell reports

authors： Suzuki T,Yoshioka T,Hara Y,Tabata M,Fujita Y

更新日期：1987-06-01 00:00:00

abstract：KEY MESSAGE:A multi-parallel approach gauging the mass spectrometry-based metabolite fingerprinting coupled with bioactivity and pathway evaluations could serve as an efficacious tool for inferring plant taxonomic orders. Thirty-four species from three plant families, namely Cornaceae (7), Fabaceae (9), and Rosaceae (1...

journal_title：Plant cell reports

authors： Son SY,Kim NK,Lee S,Singh D,Kim GR,Lee JS,Yang HS,Yeo J,Lee S,Lee CH

更新日期：2016-09-01 00:00:00