Agrobacterium induced gall formation in lipoxygenase mutant isolines of soybeans.

Abstract:

:Agrobacterium-mediated transformation frequency is very low with cells from some species such as soybeans. Studies were conducted to investigate the Agrobacterium-mediated transformation frequency in near-isogenic lipoxygenase mutant lines of soybeans, since the nigh level of lipoxygenase activity in soybean embryos might be expected to affect interactions with Agrobacterium. The mutant line lacking lipoxygenase 3 showed significantly greater frequency of Agrobacterium-induced transformation than the other soybean lines. Stages of soybean embryo development which showed maximum differences in lipoxygenase 3 activity between mutant and wild-type, also showed maximum differences in transformation frequency. The increased transformation frequency with the absence of lipoxygenase 3 was only seen when both lipoxygenase 1 and 2 were present.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Bhatt D,Parrott WA,Collins GB,Hildebrand DF

doi

10.1007/BF00231809

subject

Has Abstract

pub_date

1991-03-01 00:00:00

pages

651-4

issue

11

eissn

0721-7714

issn

1432-203X

journal_volume

9

pub_type

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