Abstract:
:Agrobacterium-mediated transformation frequency is very low with cells from some species such as soybeans. Studies were conducted to investigate the Agrobacterium-mediated transformation frequency in near-isogenic lipoxygenase mutant lines of soybeans, since the nigh level of lipoxygenase activity in soybean embryos might be expected to affect interactions with Agrobacterium. The mutant line lacking lipoxygenase 3 showed significantly greater frequency of Agrobacterium-induced transformation than the other soybean lines. Stages of soybean embryo development which showed maximum differences in lipoxygenase 3 activity between mutant and wild-type, also showed maximum differences in transformation frequency. The increased transformation frequency with the absence of lipoxygenase 3 was only seen when both lipoxygenase 1 and 2 were present.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Bhatt D,Parrott WA,Collins GB,Hildebrand DFdoi
10.1007/BF00231809subject
Has Abstractpub_date
1991-03-01 00:00:00pages
651-4issue
11eissn
0721-7714issn
1432-203Xjournal_volume
9pub_type
杂志文章abstract::The sugarcane (Saccharum officinarum) stress-related MYB transcription factor gene, ScMYBAS1, demonstrated induced response to water deficit and salt stress in our previous study. To elucidate its stress tolerance mechanism at the transcriptional level, we isolated and characterized the promoter (PScMYBAS1, 1,033 bp) ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1183-y
更新日期:2012-04-01 00:00:00
abstract::Two clones of Populus nigra L. were tested in vivo and in vitro for their susceptibility to three different Agrobacterium tumefaciens wild-type strains evaluating number and size of resulting calluses. Strain C58 proved to be the most virulent.Various parameters affecting Agrobacterium-mediated transformation of P. ni...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233315
更新日期:1994-02-01 00:00:00
abstract::The present protocol was aimed at establishing a routine transformation procedure via Agrobacterium tumefaciens for an important Oncidium orchid cultivar. An expression vector containing hptII and gusA genes driven by the cauliflower mosaic virus (CaMV) 35S promoter was successfully introduced into the Oncidium orchid...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0614-9
更新日期:2003-06-01 00:00:00
abstract::Time-course changes in anthocyanin accumulation, phenylalanine ammonia-lyase activity and chalcone synthase activity were examined in roselle callus tissues incubated under different culture conditions. Phenylalanine ammonia-lyase activity was not affected by either the kind of auxin supplemented to the medium or ligh...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269050
更新日期:1989-12-01 00:00:00
abstract::To develop an Agrobacterium mediated transformation protocol for chrysanthemum we studied the transformation efficiency of commonly used A.tumefaciens strains on 14 genotypes by comparing tumour size and frequency. One genotype was analyzed in detail using 14 strains of both A.tumefaciens and A.rhizogenes. Only a few ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232106
更新日期:1991-01-01 00:00:00
abstract::Mitogen-activated protein kinase (MAPK) signaling cascades are evolutionarily conserved fundamental signal transduction pathways. A MAPK cascade consists of many distinct MAPKKK-MAPKK-MAPK modules linked to various upstream receptors and downstream targets through sequential phosphorylation and activation of the casca...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-013-1437-y
更新日期:2013-06-01 00:00:00
abstract::The variegated Saintpaulia cultivar Thamires (Saintpaulia sp.), which has pink petals with blue splotches, is generally maintained by leaf cuttings. In contrast, tissue culture-derived progeny of the cultivar showed not only a high percentage of mutants with solid-blue petals but also other solid-color variants, which...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1016-z
更新日期:2011-05-01 00:00:00
abstract::Conditions favourable to embryogenesis from isolated microspores of Brassica rapa L. ssp. oleifera (canola quality) were identified. A population with enhanced responsiveness for microspore embryogenesis (C200) was synthesized by crossing individual plants showing microspore embryogenic potential. For optimal microspo...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232537
更新日期:1992-05-01 00:00:00
abstract::Plant regeneration via somatic embryogenesis was achieved in callus cultures derived from nodal explants of in vitro grown seedlings and excised mature zygotic embryos of three bamboo species on Murashige and Skoog's (MS) basal medium supplemented with 0.5 mg/l kinetin (Kn), 2.0 mg/l 2,4-dichlorophenoxyacetic acid (2,...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231624
更新日期:1994-09-01 00:00:00
abstract:: Mature ovules of Dianthus (Caryophyllaceae) were histologically observed by clearing and serial sectioning to characterize the cells of the embryo sac. The results show that the mature embryo sac was located deep inside the hemitropous ovule due to thick nucellar tissue at the micropylar region. For the isolation of ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050753
更新日期:2000-04-01 00:00:00
abstract::Transgenic calli of Pharbitis nil which grow in the presence of kanamycin were obtained by introduction of plasmid pBI121, which carries kanamycin resistance and the gene for beta-glucuronidase. The calli were shown to have fragments of vector DNA in their genome and high levels of beta-glucuronidase activity. This is...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00274124
更新日期:1989-05-01 00:00:00
abstract::Genetic transformation of arctic bramble (Rubus arcticus L.) was achieved utilizing a Ti-plasmid vector system of Agrobacterium tumefaciens. Internodal stem segments were inoculated with Agrobacterium strain EHA101 carrying a T-DNA with the CaMV 35 S promoter-gus-int marker gene from which β-glucuronidase (GUS) is exp...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050491
更新日期:1998-07-01 00:00:00
abstract::We have established a high-frequency plant regeneration system via somatic embryogenesis from mature seeds of creeping bentgrass (Agrostis palustris Huds). The effects of 2,4-dichlorophenoxyacetic acid (2,4-D), 3.6-dichloroo-anisic acid (dicamba) and 6-benzyladenine (BA) on callus formation and embryogenesis were eval...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233813
更新日期:1991-11-01 00:00:00
abstract::Fully fertile plants with the expected chromosome number 2n=40 were regenerated from excised leaf sections of Glycine clandestina. Shoots formed directly on the explants through organogenesis. Utilizing the same media and procedures fully fertile plants were also regenerated from cotyledon and hypocotyl tissue of the ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269116
更新日期:1986-06-01 00:00:00
abstract::Cell suspension cultures of bean (Phaseolus vulgaris) cv. Imuna accumulated isoflavonoid phytoalexins on exposure to elicitor from the phytopathogenic fungus Colletotrichum lindemuthianum (CL). This was preceeded by rapid increases in the activities of phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS). How...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269060
更新日期:1989-12-01 00:00:00
abstract:KEY MESSAGE:A R2R3-MYB transcription factor EsAN2 was isolated from Epimedium sagittatum and functionally characterized to regulate the anthocyanin biosynthetic pathway. Epimedium plants are used widely both as traditional Chinese medicinal herbs and ornamental perennials. Anthocyanins, acting as major contributors to ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1929-z
更新日期:2016-04-01 00:00:00
abstract::Shoot and leaf segments of a non-regenerable Medicago sativa L. genotype were cocultivated with the "shooty" mutant of Agrobacterium tumefaciens carrying the pGV 2206 plasmid. Transformed callus lines were selected and regenerated on the hormone free B5 medium. Southern blot analysis demonstrated integration of T-DNA ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269987
更新日期:1990-04-01 00:00:00
abstract::Hairy root cultures of Lithospermum canescens were established using three strains of Agrobacterium rhizogenes: ATCC 15834, LBA 9402 and NCIB 8196. Eight lines resulting from infection with A. rhizogenes ATCC 15834 demonstrated sufficient biomass increase and were submitted to further investigations. The contents of a...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0161-2
更新日期:2006-10-01 00:00:00
abstract:KEY MESSAGE:Phytophthora infestans resistant somatic hybrids of S. × michoacanum (+) S. tuberosum and autofused 4 x S. × michoacanum were obtained. Our material is promising to introgress resistance from S. × michoacanum into cultivated potato background. Solanum × michoacanum (Bitter.) Rydb. (mch) is a wild diploid (2...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-013-1422-5
更新日期:2013-08-01 00:00:00
abstract:KEY MESSAGE:The Arabidopsis receptor-like cytoplasmic kinase AtRLCK VI_A3 is activated by AtROPs and is involved in trichome branching and pathogen interaction. Receptor-like cytoplasmic kinases (RLCKs) belong to the large superfamily of receptor-like kinases, which are involved in a variety of cellular processes like ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1725-1
更新日期:2015-03-01 00:00:00
abstract::Tef [Eragrostis tef (Zucc.) Trotter] is the most important cereal in Ethiopia. In its wild relative E. mexicana, regeneration of six green plants resulted from culture of 121 non-pollinated immature pistils. In the allotetraploid crop species tef, however, only callus and root formation was obtained by this method. By...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0200-z
更新日期:2006-12-01 00:00:00
abstract::The introgression of new desirable characters is very necessary in common bean (Phaseolus vulgaris L.)Interspecific hybridization of P. vulgaris L. with P. angustissimus A. Gray, a wild species with narrow leaves, could be successfully achieved for the first time using embryo culture. Sixteen to twenty-three-day old e...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00268593
更新日期:1986-10-01 00:00:00
abstract::Transgenic plants offer advantages for biomolecule production because plants can be grown on a large scale and the recombinant macromolecules can be easily harvested and extracted. We introduced an Aspergillus phytase gene into canola (Brassica napus) (line 9412 with low erucic acid and low glucosinolates) by Agrobact...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0036-y
更新日期:2006-03-01 00:00:00
abstract::DNA transfer into zygotic embryos of Eucalyptus globulus by microprojectile bombardment was studied with two devices: a gunpowder apparatus and a compressed-helium system. Using, as a test, the transient expression of a reporter gene, we optimized the physical and biological conditions of bombardment. Six-day-old cult...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232737
更新日期:1995-07-01 00:00:00
abstract:KEY MESSAGE:Candidate genes were identified and the role of phytohormones such as JA-Me and ABA in the synthesis of S-RNase was emphasized in pear self-incompatibility. Self-incompatibility (SI) occurs widely in flowering plants as an intraspecific reproductive barrier. This phenomenon promotes variation within species...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-017-2194-0
更新日期:2017-11-01 00:00:00
abstract::A protocol for whole plant regeneration of Cicer arietinum L. cv. C-235 via organogenesis from callus has been developed. Callus initiation was best when immature leaflets were cultured on MS medium containing 5 or 25 μM 2,4-D or NAA in combination with 10 μM BA, or 25 μM 2,4-D alone. The callus grew most vigorously o...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232946
更新日期:1994-06-01 00:00:00
abstract::Callus cultures of Vitis labruscana cv. Concord were initiated from both immature fruit and petiole tissue. Analysis revealed that the acid hydrolyzable precursors to β-damascenone, a compound important to the flavor of most grape species, were present in both callus types. Light and increased sucrose concentration we...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231971
更新日期:1994-05-01 00:00:00
abstract::Embryogenic callus and suspension cultures of carrot (Daucus carota L., cv. Nantaise), growing on/in medium including 1 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D), were transferred to medium with or without this plant growth regulator, to impair or induce, respectively, further development of somatic embryos. The en...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-004-0869-9
更新日期:2005-01-01 00:00:00
abstract::Protoplasts were isolated from embryogenic callus ofAbies alba L. which originated from immature seeds. The protoplasts were immobilized in alginate beads in order to follow the development of single protoplasts.Surrounding culture medium was modified from Kao and Michayluk (1975). After cell wall regeneration subsequ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00716854
更新日期:1989-02-01 00:00:00
abstract::Chrysosplenium americanum (Saxifragaceae) accumulates a variety of partially O-methylated flavonol glucosides. Because of the semi-aquatic nature of this plant and its extensive contamination with endogenous organisms, the initiation of shoot and callus cultures could only be achieved after (a) using a special surface...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00270122
更新日期:1988-03-01 00:00:00