Abstract:
:Time-course changes in anthocyanin accumulation, phenylalanine ammonia-lyase activity and chalcone synthase activity were examined in roselle callus tissues incubated under different culture conditions. Phenylalanine ammonia-lyase activity was not affected by either the kind of auxin supplemented to the medium or light regime. In contrast, chalcone synthase activity was markedly suppressed when the callus was cultured with a medium containing indole-3-acetic acid instead of 2,4-dichlorophenoxyacetic acid (2,4-D) or in the dark. The results imply that in roselle callus cultures chalcone synthase plays a more important role in anthocyanin biosynthesis regulated by 2,4-D and light irradiation than phenylalanine ammonialyase.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Mizukami H,Tomita K,Ohashi Hdoi
10.1007/BF00269050subject
Has Abstractpub_date
1989-12-01 00:00:00pages
467-70issue
8eissn
0721-7714issn
1432-203Xjournal_volume
8pub_type
杂志文章abstract:KEY MESSAGE:We provide evidence that nucleotide sequence and methylation status changes occur in the Arabidopsis genome during in vitro tissue culture at a frequency high enough to represent an important source of variation. Somaclonal variation is a general consequence of the tissue culture process that has to be anal...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-017-2217-x
更新日期:2018-01-01 00:00:00
abstract::Most infections of plant roots are initiated in the region of elongation; the mechanism for this tissue-specific localization pattern is unknown. In alfalfa expressing PsUGT1 antisense mRNA under the control of the cauliflower mosaic virus (CaMV) 35S promoter, the cell cycle in roots is completed in 48 h instead of 24...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-004-0787-x
更新日期:2004-07-01 00:00:00
abstract::Ma bamboo (Dendrocalamus latiflorus Munro) belongs to Dendrocalamus genus, Bambusease tribe, Bambusoideae subfamily, Poaceae family. It is a representative species of clumping bamboo, and a principal commercial species for various construction purposes using mature culms and for human consumption using young shoots. A...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1112-0
更新日期:2011-11-01 00:00:00
abstract::Various inhibitors of polyamine biosynthesis were used to study the role of polyamines in DNA synthesis and cell division in suspension cultures of Catharanthus roseus (L.) G. Don. Arginine decarboxylase (ADC; EC 4.1.1.19) was the major enzyme responsible for putrescine production. DL α-difluoromethylarginine inhibite...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232042
更新日期:1991-06-01 00:00:00
abstract::TransgenicRhododendron plants were obtained byAgrobacterium tumefaciens-mediated gene transfer.A. tumefaciens harboring a binary vector that contained the chimeric neomycin phosphotransferase II (NPTII) and (3-glucuronidase (GUS) genes was co-cultivated with stem and leaf segments fromRhododendron tissues culturedin v...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01275445
更新日期:1996-11-01 00:00:00
abstract::A procedure for inducing somatic embryos in shoot apex explants (2 mm) excised from shoot proliferation cultures established from adult oak trees (Quercus robur) was investigated. Embryogenesis was induced in shoot tip as well as leaf explants in three out of the five genotypes evaluated. Somatic embryos were formed b...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0852-6
更新日期:2010-06-01 00:00:00
abstract::The present protocol was aimed at establishing a routine transformation procedure via Agrobacterium tumefaciens for an important Oncidium orchid cultivar. An expression vector containing hptII and gusA genes driven by the cauliflower mosaic virus (CaMV) 35S promoter was successfully introduced into the Oncidium orchid...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0614-9
更新日期:2003-06-01 00:00:00
abstract::This report describes the successful plant regeneration via somatic embryogenesis from immature zygotic embryos of Cryptomeria japonica D. Don. For the induction of embryogenic tissue, we determined that the optimal medium contained N6-benzyladenine and 2,4-dichlorophenoxyacetic acid. Immature zygotic embryos that wer...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0687-5
更新日期:2003-11-01 00:00:00
abstract::Embryogenic callus and suspension cultures of carrot (Daucus carota L., cv. Nantaise), growing on/in medium including 1 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D), were transferred to medium with or without this plant growth regulator, to impair or induce, respectively, further development of somatic embryos. The en...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-004-0869-9
更新日期:2005-01-01 00:00:00
abstract::Conditions favourable to embryogenesis from isolated microspores of Brassica rapa L. ssp. oleifera (canola quality) were identified. A population with enhanced responsiveness for microspore embryogenesis (C200) was synthesized by crossing individual plants showing microspore embryogenic potential. For optimal microspo...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232537
更新日期:1992-05-01 00:00:00
abstract::Methods of shoot and plant development from cotyledon explants of the tomato cultivar 'Bonner Beste' and its mutant chloronerva are described. Cotyledon mesophyll protoplasts of the mutant chloronerva have been isolated and cultured, and induced to regenerate shoots and plants. By grafting regenerated shoots of the ch...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00270102
更新日期:1983-08-01 00:00:00
abstract::Arabidopsis thaliana protoplasts, isolated from a cell suspension, regenerated cell walls and then exhibited sustained cell divisions. Within 10 days after isolation as many as 40% of the protoplast-derived cells divided. Protoplast-derived calli exhibited a wide range of developmental responses, including apparent em...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00820203
更新日期:1990-01-01 00:00:00
abstract::The direct induction of adventitious shoots from leaf explants obtained from adult plants of Zhanhua winter jujube, an elite variety of Zizyphus jujuba Mill., is reported. The percentage of leaf explants producing shoots and the average number of shoots per explant were significantly improved when 10-day-old leaves we...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0920-5
更新日期:2005-03-01 00:00:00
abstract::A genetic transformation protocol for green ash (Fraxinus pennsylvanica) hypocotyl explants was developed. Green ash hypocotyls were transformed using Agrobacterium tumefaciens strain EHA105 harboring binary vector pq35GR containing the neomycin phosphotransferase (nptII) and beta-glucuronidase (GUS) fusion gene, and ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-009-0697-z
更新日期:2009-06-01 00:00:00
abstract::Cell suspension cultures of Nicotiana were initiated using conditions designed to selectively favor stable chromosome number. These conditions included use of leaf explants to initiate cultures, growth of cells in culture medium containing 2,4-D, and transfer of cells with short subculture intervals. Four cell lines d...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272364
更新日期:1982-04-01 00:00:00
abstract:KEY MESSAGE:Marker-free transgenic plants can be generated with high efficiency by using the Cre/ lox P self-excision system controlled by the pollen- and embryo-specific Arabidopsis DLL promoter. In this work, we aimed to study the feasibility of using the pollen- and embryo-specific DLL promoter of the At4g16160 gene...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1726-0
更新日期:2015-03-01 00:00:00
abstract::Leaf mesophyll protoplasts isolated from pea (Pisum sativum L.) genotypes Century and PI244253 showed transient expression of β-glucuronidase (GUS) when electroporated with plasmid DNA containing various promoter-leader sequence constructs driving the GUS gene. The optimum conditions for transient expression were: usi...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232127
更新日期:1990-06-01 00:00:00
abstract::Surface-sterilized leaf disks of horse-radish (Armoracia lapathifolia) were immersed in a suspension of Agrobacterium rhizogenes harboring the root-inducing plasmid (pRi) and cultured on a solid medium. Within about 10 days after inoculation, adventitious roots (hairy roots) emerged from the leaf disks. No roots emerg...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00271999
更新日期:1987-07-01 00:00:00
abstract:KEY MESSAGE:78 HD-Zip family genes in Phyllostachys edulis were analyzed. Overexpression of Phehdz1 can improve the drought tolerance of transgenic rice and affect its secondary metabolism. Many studies suggested homeodomain-leucine zipper (HD-Zip) transcription factors are important regulators of plant growth and deve...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-020-02625-w
更新日期:2021-01-01 00:00:00
abstract::Maize is one of the more important agricultural crops in the world and, under certain conditions, prone to attack from pathogenic fungi. One of these, Aspergillus flavus, produces toxic and carcinogenic metabolites, called aflatoxins, as byproducts of its infection of maize kernels. The alpha-amylase of A. flavus is k...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0250-2
更新日期:2007-04-01 00:00:00
abstract:KEY MESSAGE:Transcriptome analysis of barley embryogenic callus from isolated microspore culture under salt stress uncovered a role of translation inhibition and selective activation of stress-specific proteins in cellular defense. Soil salinity is one of the major abiotic stresses which constrains the plant growth and...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-016-1986-y
更新日期:2016-08-01 00:00:00
abstract::The aim of this study was to optimize the in vitro chromosome-doubling procedure in wheat anther culture. Colchicine, at concentrations of 100-5000 mg/l, was added to the induction medium for 1-5 days. Beneficial effects were obtained with concentrations of 100 and 1000 mg/l colchicine. With time, significant reductio...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050520
更新日期:1998-09-01 00:00:00
abstract::A procedure has been developed for the clonal propagation of Picrorhiza kurroa Royle ex Benth. through shoot tip culture. Murashige and Skoog's medium (1962) supplemented with kinetin (3.0 to 5.0 mg/l) supported rapid proliferation of multiple shoots from the explants. Addition of indole-3-acetic acid (1.0 mg/l) to th...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269324
更新日期:1988-05-01 00:00:00
abstract::Shoot cultures ofAtractylodes lancea DC. (Compositae) have been established by inoculating the flower bud on Linsmaier-Skoog's medium supplemented with 10(-5) M naphthaleneacetic acid and 10(-5) M 6-benzyl-aminopurine. Shoots were multiplied on a medium containing 10(-6) M 6-benzylaminopurine. Propagated shoots rooted...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF02441005
更新日期:1984-06-01 00:00:00
abstract::Novel and efficient protocols for plant regeneration and genetic transformation from longitudinally-halved cotyledons ofin vitro raised seedlings in eggplant (Solanum melongena L.) are described. After co-cultivation withAgrobacterium vectors harboring neomycin phosphotransferase (nptll) as selectable marker, transgen...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01690259
更新日期:1995-01-01 00:00:00
abstract:KEY MESSAGE:A novel Zoysia japonica salt-induced glycine-rich RNA-binding protein gene was cloned in this study and its overexpression caused salt sensitivity in transgenic Arabidopsis. Glycine-rich RNA-binding proteins (GRPs) play crucial roles in diverse plant developmental processes. However, the mechanisms and func...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-016-2068-x
更新日期:2017-01-01 00:00:00
abstract::Protoclones and aggregate clones have been prepared from 5 callus strains of C. ternata chosen for their dihydrofuroquinoline-accumulating capacities in a population of well-established strains. The results show that it is possible to obtain cell lines which accumulate higher alkaloid contents than the highest alkaloi...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269565
更新日期:1987-10-01 00:00:00
abstract::In order to construct transgenic rice plant with an introduced oryzacystatin (OC)-β-glucuronidase (GUS) fusion gene, we first introduced it into rice protoplasts by electroporation, together with a marker gene conferring hygromycinresistance (pUC-HPH). In a transient assay using the transfected protoplasts, both OC an...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00193714
更新日期:1995-12-01 00:00:00
abstract::The influence of light on the expression of NADPH: protochlorophyllide oxidoreductase has been studied in different plant species. The presumptive precursors to this enzyme have been characterized by in vitro translation of poly (A) RNA and immunoprecipitation. Two bands of apparent molecular weights of about 42 000 a...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269229
更新日期:1983-02-01 00:00:00
abstract::Recent developments in modern biotechnology such as the use of RNA interference (RNAi) have broadened the scope of crop genetic modification. RNAi strategies have led to significant achievements in crop protection against biotic and abiotic stresses, modification of plant traits, and yield improvement. As RNAi-derived...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-019-02446-6
更新日期:2019-12-01 00:00:00