Genetic transformation ofRhododendron byAgrobacterium tumefaciens.

Abstract:

:TransgenicRhododendron plants were obtained byAgrobacterium tumefaciens-mediated gene transfer.A. tumefaciens harboring a binary vector that contained the chimeric neomycin phosphotransferase II (NPTII) and (3-glucuronidase (GUS) genes was co-cultivated with stem and leaf segments fromRhododendron tissues culturedin vitro. Adventitious buds were fonned and shoots were regenerated on kanamycin selection medium 3-4 months after inoculation. Integration of the NPTII and the GUS genes was confirmed by polymerase chain reaction (PCR) and by Southern hybridization analyses. Histochemical GUS assay showed that the inserted gene was expressed in all tissues with the cauliflower mosaic virus (CaMV) 35S promoter. This transformation procedure has the potential to expand the range of genetic variation inRhododendron.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Ueno K,Fukunaga Y,Arisumi K

doi

10.1007/BF01275445

subject

Has Abstract

pub_date

1996-11-01 00:00:00

pages

38-41

issue

1-2

eissn

0721-7714

issn

1432-203X

journal_volume

16

pub_type

杂志文章
  • Lanthanide ions are agonists of transient gene expression in rice protoplasts and act in synergy with ABA to increase Em gene expression.

    abstract::Previous work has shown that in rice suspension cells, NaCl at 0.4 M can induce Em gene expression and act synergistically with ABA, possibly by potentiating the ABA response pathway through a rate-limiting intermediate (R.M. Bostock and R.S. Quatrano (1992) Plant Physiol., 98, 1356-1363). Since calcium is an intermed...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232374

    authors: Rock CD,Quatrano RS

    更新日期:1996-01-01 00:00:00

  • Efficient plant regeneration from hypocotyl protoplasts of broccoli (Brassica oleracea L. ssp. italica Plenck).

    abstract::We have assessed the capacity of cultured protoplasts from two tissue sources of several commercially-grown broccoli cultivars to regenerate plants. A procedure that employs hypocotyl protoplasts and a culture medium with a high NAA:2,4-D auxin ratio was developed. The procedure permits highly efficient formation of c...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232858

    authors: Kao HM,Keller WA,Gleddie S,Brown GG

    更新日期:1990-10-01 00:00:00

  • Microcallus formation from leaf mesophyll protoplasts in the genus Actinidia Lindl.

    abstract::Microcallus (more than 60 cells) formation was obtained from leaf mesophyll protoplasts of 6 species and varieties in the genus Actinidia Lindl. (kiwifruit). The best results were achieved by using liquid over agarose culture for A. arguta var. arguta, liquid and agarose disc type culture for A. arguta var. purpurea, ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00231583

    authors: Xiao XG,Hirsch AM

    更新日期:1996-09-01 00:00:00

  • Polyamines and crown gall tumor growth.

    abstract::The effect of the polyamine spermidine on the growth of crown gall tumors was determined using the potato disc bioassay. Addition of lmM spermidine resulted in a 30-50% increase in tumor growth. The spermidine effect was found to be biphasic, with lmM being optimal. Closely related polyamines including spermine, as we...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269212

    authors: Kulpa JM,Galsky AG,Lipetz P,Stephens R

    更新日期:1985-04-01 00:00:00

  • Arabidopsis GIGANTEA negatively regulates chloroplast biogenesis and resistance to herbicide butafenacil.

    abstract:KEY MESSAGE:Arabidopsis GI negatively regulates chloroplast biogenesis and resistance to the herbicide butafenacil by enhanced activity and transcriptional levels of antioxidant enzymes Chloroplast biogenesis is blocked by retrograde signaling triggered by diverse internal and external cues, including sugar, reactive o...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-019-02409-x

    authors: Cha JY,Lee DY,Ali I,Jeong SY,Shin B,Ji H,Kim JS,Kim MG,Kim WY

    更新日期:2019-07-01 00:00:00

  • MsZEP, a novel zeaxanthin epoxidase gene from alfalfa (Medicago sativa), confers drought and salt tolerance in transgenic tobacco.

    abstract:KEY MESSAGE:The zeaxanthin epoxidase gene ( MsZEP ) was cloned and characterized from alfalfa and validated for its function of tolerance toward drought and salt stresses by heterologous expression in Nicotiana tabacum. Zeaxanthin epoxidase (ZEP) plays important roles in plant response to various environment stresses d...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-015-1895-5

    authors: Zhang Z,Wang Y,Chang L,Zhang T,An J,Liu Y,Cao Y,Zhao X,Sha X,Hu T,Yang P

    更新日期:2016-02-01 00:00:00

  • Generation and analysis of expressed sequence tags from a normalized cDNA library of young leaf from Ma bamboo (Dendrocalamus latiflorus Munro).

    abstract::Ma bamboo (Dendrocalamus latiflorus Munro) belongs to Dendrocalamus genus, Bambusease tribe, Bambusoideae subfamily, Poaceae family. It is a representative species of clumping bamboo, and a principal commercial species for various construction purposes using mature culms and for human consumption using young shoots. A...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-011-1112-0

    authors: Gao ZM,Li CL,Peng ZH

    更新日期:2011-11-01 00:00:00

  • Genome-wide transcriptional response of papain-like cysteine protease-mediated resistance against Xanthomonas oryzae pv. oryzae in rice.

    abstract:KEY MESSAGE:Transgenic rice overexpressing PLCP attenuated the virulence of Xanthomonas oryzae pv. oryzae through extensive activation of transduction signal and transcription activities that orchestrate downstream responses including the biosynthesis of secondary metabolites and up-regulation of several pathogenesis-r...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-019-02502-1

    authors: Niño MC,Kang KK,Cho YG

    更新日期:2020-04-01 00:00:00

  • The utility of green fluorescent protein in transgenic plants.

    abstract::The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has proven to be a powerful tool in plant genetic transformation studies. This paper reviews the history and the progression of the expression of GFP variants in transgenic plants. The distinguishing features of the most useful GFPs, such as thos...

    journal_title:Plant cell reports

    pub_type: 杂志文章,评审

    doi:10.1007/s002990100346

    authors: Stewart CN Jr

    更新日期:2001-07-01 00:00:00

  • Agrobacterium induced gall formation in lipoxygenase mutant isolines of soybeans.

    abstract::Agrobacterium-mediated transformation frequency is very low with cells from some species such as soybeans. Studies were conducted to investigate the Agrobacterium-mediated transformation frequency in near-isogenic lipoxygenase mutant lines of soybeans, since the nigh level of lipoxygenase activity in soybean embryos m...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00231809

    authors: Bhatt D,Parrott WA,Collins GB,Hildebrand DF

    更新日期:1991-03-01 00:00:00

  • Transient gene expression after electroporation of protoplasts derived from embryogenic maize callus.

    abstract::Protoplasts isolated from embryogenic callus cultures derived from immature embryos ofZea mays L. are suitable for analysis of transient gene expression using electroporation-mediated DNA transfer. Expression of introduced genes is comparable to the levels obtained with protoplasts from Black Mexican Sweet suspension ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00716827

    authors: Planckaert F,Walbot V

    更新日期:1989-03-01 00:00:00

  • Heterologous expression of a novel Zoysia japonica salt-induced glycine-rich RNA-binding protein gene, ZjGRP, caused salt sensitivity in Arabidopsis.

    abstract:KEY MESSAGE:A novel Zoysia japonica salt-induced glycine-rich RNA-binding protein gene was cloned in this study and its overexpression caused salt sensitivity in transgenic Arabidopsis. Glycine-rich RNA-binding proteins (GRPs) play crucial roles in diverse plant developmental processes. However, the mechanisms and func...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-016-2068-x

    authors: Teng K,Tan P,Xiao G,Han L,Chang Z,Chao Y

    更新日期:2017-01-01 00:00:00

  • Heading date gene, dth3 controlled late flowering in O. Glaberrima Steud. by down-regulating Ehd1.

    abstract::Heading date in rice is an important agronomic trait controlled by several genes. In this study, flowering time of variety Dianjingyou 1 (DJY1) was earlier than a near-isogenic line (named NIL) carried chromosome segment from African rice on chromosome 3S, when grown in both long-day (LD) and short-day (SD) conditions...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-011-1129-4

    authors: Bian XF,Liu X,Zhao ZG,Jiang L,Gao H,Zhang YH,Zheng M,Chen LM,Liu SJ,Zhai HQ,Wan JM

    更新日期:2011-12-01 00:00:00

  • Sugarcane protoplasts: factors affecting division and plant regeneration.

    abstract::Sugarcane cell suspensions were initiated from leaf callus and sub-cultured every 7 to 10 days by alternate transfer to MS based medium with 3.0 and 1.0 mg 1(-1)2,4-D. Suspensions older than 3 months gave the most reproducible yields of protoplasts. Isolated protoplasts required 50 mM Ca(2+) in the washing solution an...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269934

    authors: Chen WH,Davey MR,Power JB,Cocking EC

    更新日期:1988-08-01 00:00:00

  • Effect of cytokinin on morphological changes of suspension cultured cells of the moss, Barbula unguiculata.

    abstract::Suspension cultured cells of the moss, Barbula unguiculata, grow actively in both light and dark culture. Light-grown cells contain chlorophyll and exhibit an undifferentiated callus form. When cells are transferred to a dark condition, they develop into protonemata. Protonemata formation in the dark can be inhibited ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00272039

    authors: Takio S

    更新日期:1989-03-01 00:00:00

  • Gene silencing studies in the gymnosperm species Pinus radiata.

    abstract::A biolistic transformation procedure was used to transform embryogenic Pinus radiata tissue with constructs containing the Zea mays UBI1 (ubiquitin)-promoter followed by the P. radiata CAD (cinnamyl alcohol dehydrogenase) cDNA in sense or anti-sense orientation or in the form of an inverted-repeat. The effect of the d...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-004-0911-y

    authors: Wagner A,Phillips L,Narayan RD,Moody JM,Geddes B

    更新日期:2005-05-01 00:00:00

  • Selection for NaCl tolerance in cell cultures of Medicago sativa and recovery of plants from a NaCl-tolerant cell line.

    abstract::Medicago sativa lines with a high incidence of regeneration were established as suspension cultures and used to select for NaCl tolerant lines. Attempts were then made to regenerate plants from these lines. Regeneration was severely depressed in NaCl tolerant calli and the only plants that were successfully regenerate...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269335

    authors: Smith MK,McComb JA

    更新日期:1983-06-01 00:00:00

  • Selection of large quantities of embryogenic calli from indica rice seeds for production of fertile transgenic plants using the biolistic method.

    abstract::The microprojectile bombardment of immature embryos has proven to be effective in transforming many indica rice varieties. One of the drawbacks of using immature embryos is the requirement of a large number of high quality immature embryos, which itself is a tedious and laborious process. To circumvent these problems,...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232364

    authors: Sivamani E,Shen P,Opalka N,Beachy RN,Fauquet CM

    更新日期:1996-01-01 00:00:00

  • Genotypic and developmental regulation of transient expression of a reporter gene in soybean zygotic cotyledons.

    abstract::Processes involved in transformation of regenerable soybean (Glycine max (L.) Merrill) immature zygotic cotyledons were studied by assaying the transient expression of the β-glucuronidase gene driven by the 35S promoter and terminated at the 3' end by the soybean 7S storage protein gene. The plasmid containing the chi...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00234510

    authors: Moore PJ,Moore AJ,Collins GB

    更新日期:1994-07-01 00:00:00

  • Chromosome stability of cell suspension cultures of Nicotiana spp.

    abstract::Cell suspension cultures of Nicotiana were initiated using conditions designed to selectively favor stable chromosome number. These conditions included use of leaf explants to initiate cultures, growth of cells in culture medium containing 2,4-D, and transfer of cells with short subculture intervals. Four cell lines d...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00272364

    authors: Evans DA,Gamborg OL

    更新日期:1982-04-01 00:00:00

  • Rapid micropropagation for Mucuna pruriens f. pruriens L.

    abstract::A rapid micropropagation system was developed for Mucuna pruriens f. pruriens using explants from 1-week-old aseptically grown seedlings. Multiple shoot regeneration occurred following an initial callus growth on Revised Tobacco (RT) medium supplemented with 2.7 μM NAA and 9.8 μM 2iP. Maximum number of shoot regenerat...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00193734

    authors: Chattopadhyay S,Datta SK,Mahato SB

    更新日期:1995-12-01 00:00:00

  • Leaf nitrogen dioxide uptake coupling apoplastic chemistry, carbon/sulfur assimilation, and plant nitrogen status.

    abstract::Emission and plant uptake of atmospheric nitrogen oxides (NO + NO(2)) significantly influence regional climate change by regulating the oxidative chemistry of the lower atmosphere, species composition and the recycling of carbon and nutrients, etc. Plant uptake of nitrogen dioxide (NO(2)) is concentration-dependent an...

    journal_title:Plant cell reports

    pub_type: 杂志文章,评审

    doi:10.1007/s00299-010-0898-5

    authors: Hu Y,Sun G

    更新日期:2010-10-01 00:00:00

  • Interspecific somatic hybrids between Solanum khasianum and S. aculeatissimum produced by electrofusion.

    abstract::Mesophyll derived protoplasts of Solanum khasianum Clarke and S. aculeatissimum Jacq. were fused by electrofusion and resulted in interspecific hybrids. In total, 249 calluses were obtained from which more than 65% produced shoots. From the regenerated plantlets 45% could be identified as hybrids by chromosome counts ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00239891

    authors: Stattmann M,Gerick E,Wenzel G

    更新日期:1994-01-01 00:00:00

  • Level of tissue differentiation influences the activation of a heat-inducible flower-specific system for genetic containment in poplar (Populus tremula L.).

    abstract:KEY MESSAGE:Differentiation level but not transgene copy number influenced activation of a gene containment system in poplar. Heat treatments promoted CRE gene body methylation. The flower-specific transgene deletion was confirmed. Gene flow between genetic modified trees and their wild relatives is still motive of con...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-015-1890-x

    authors: Hoenicka H,Lehnhardt D,Nunna S,Reinhardt R,Jeltsch A,Briones V,Fladung M

    更新日期:2016-02-01 00:00:00

  • The ornamental variety, Japanese striped corn, contains high anthocyanin levels and PAL specific activity: establishing the potential for development of an oral therapeutic.

    abstract::Phenylalanine ammonia-lyase [PAL, EC 4.3.1.24 (formerly EC 4.3.1.5)], functions in the plant phenylpropanoid biosynthetic pathway to deaminate the amino acid L-phenylalanine forming trans-cinnamic acid and ammonia. The human inherited metabolic disorder phenylketonuria (PKU) is characterized by an inability of individ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-008-0650-6

    authors: McInnis S,Clemens S,Kermode AR

    更新日期:2009-03-01 00:00:00

  • Control of foreign polypeptide localization in specific layers of protein body type I in rice seed.

    abstract:KEY MESSAGE:Prolamin-GFP fusion proteins, expressed under the control of native prolamin promoters, were localized in specific layers of PB-Is. Prolamin-GFP fusion proteins were gradually digested from outside by pepsin digestion. In rice seed endosperm, protein body type I (PB-I) has a layered structure consisting of ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-016-1960-8

    authors: Sasou A,Shigemitsu T,Saito Y,Tanaka M,Morita S,Masumura T

    更新日期:2016-06-01 00:00:00

  • Recovery of the non-functional EGFP-assisted identification of mutants generated by CRISPR/Cas9.

    abstract:KEY MESSAGE:The recovery of non-functional-enhanced green fluorescence protein can be used as indicator to facilitate the identification of mutants generated by CRISPR/Cas9. The CRISPR/Cas9 system is a powerful tool for genome editing and it has been employed to knock out genes of interest in multiple plant species. Id...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-019-02465-3

    authors: Ren C,Guo Y,Gathunga EK,Duan W,Li S,Liang Z

    更新日期:2019-12-01 00:00:00

  • Elicitation of benzophenanthridine alkaloid synthesis in Eschscholtzia cell cultures.

    abstract::Quaternary benzophenanthridine alkaloids (sanguinarine, chelerythrine, chelirubine, chelilutine and macarpine) are specifically induced by cell wall components of Penicillium and Saccharomyces in a colorless strain of Eschscholtzia californica cell suspension cultures. Classical elicitors such as the Phytophthora mega...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00272770

    authors: Schumacher HM,Gundlach H,Fiedler F,Zenk MH

    更新日期:1987-12-01 00:00:00

  • Development of a CAPS marker system for genotyping European pear cultivars harboring 17 S alleles.

    abstract::The full-length cDNAs of eight S ribonucleases (S-RNases) were cloned from stylar RNA of European pear cultivars that could not be characterized by the cleaved amplified polymorphic sequences (CAPS) marker system for genotyping European pear cultivars harboring nine S alleles Sa, Sb, Sd, Se, Sh, Sk, Sl, Sq, and Sr. Co...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-006-0254-y

    authors: Moriya Y,Yamamoto K,Okada K,Iwanami H,Bessho H,Nakanishi T,Takasaki T

    更新日期:2007-03-01 00:00:00

  • Asymbiotic in vitro seed propagation of Dendrobium.

    abstract::The ability to germinate orchids from seeds in vitro presents a useful and viable method for the propagation of valuable germplasm, maintaining the genetic heterogeneity inherent in seeds. Given the ornamental and medicinal importance of many species within the genus Dendrobium, this review explores in vitro technique...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-015-1829-2

    authors: Teixeira da Silva JA,Tsavkelova EA,Ng TB,Parthibhan S,Dobránszki J,Cardoso JC,Rao MV,Zeng S

    更新日期:2015-10-01 00:00:00