The utility of green fluorescent protein in transgenic plants.

abstract：:We have assessed the capacity of cultured protoplasts from two tissue sources of several commercially-grown broccoli cultivars to regenerate plants. A procedure that employs hypocotyl protoplasts and a culture medium with a high NAA:2,4-D auxin ratio was developed. The procedure permits highly efficient formation of c...

journal_title：Plant cell reports

authors： Kao HM,Keller WA,Gleddie S,Brown GG

更新日期：1990-10-01 00:00:00

abstract：:Protoplasts of sugarbeet (Beta vulgaris L.) were isolated from cell suspension cultures and cultured in modified PGo medium. Conditions required for the efficient division of the protoplasts were investigated.The optimal combination of phytohormones was found to be 1 mg/l NAA, 0.2 mg/l 2,4-D, 0.5 mg/l zeatin. Protopla...

journal_title：Plant cell reports

authors： Szabados L,Gaggero C

更新日期：1985-08-01 00:00:00

abstract：:KEY MESSAGE : Rooting of Artemisia annua increases trichome size on leaves and helps drive the final steps of the biosynthesis of the sesquiterpene antimalarial drug, artemisinin. ...

journal_title：Plant cell reports

authors： Nguyen KT,Towler MJ,Weathers PJ

更新日期：2013-02-01 00:00:00

abstract：KEY MESSAGE:We developed a method for detection of mutants in a large number of plants, and found this method to be applicable to detection of a mutant allele at a concentration of 1/1000. Many techniques for SNP analysis have been developed, but most of these techniques are not so sensitive to be used for detection of...

journal_title：Plant cell reports

authors： Kohata R,Koitabashi K,Kitashiba H,Nishio T

更新日期：2018-06-01 00:00:00

abstract：:Plants were regenerated from leaf explants of Centrosema brasilianum cultured in vitro. Callus and buds were produced on Murashige and Skoog medium (MS), 0.8% agar, 0.1 mg/l NAA and 1 mg/l BAP. Regeneration of multiple shoots was achieved by transferring callus onto fresh medium containing 0.01 and 1 mg/l of NAA and B...

journal_title：Plant cell reports

authors： Angeloni PN,Rey HY,Mroginski LA

更新日期：1992-09-01 00:00:00

abstract：:The expression and functionality of a resveratrol synthase (RS) gene from peanut (Arachis hypogaea) was studied using an Agrobacterium tumefaciens-mediated transient expression system in Nicotiana benthamiana leaves. Functional analysis of RS was demonstrated by tracking its expression during 96 h. To measure the tran...

journal_title：Plant cell reports

authors： Condori J,Medrano G,Sivakumar G,Nair V,Cramer C,Medina-Bolivar F

更新日期：2009-04-01 00:00:00

abstract：:The use of antibiotic or herbicide resistant genes as selection markers for production of transgenic plants and their continuous presence in the final transgenics has been a serious problem for their public acceptance and commercialization. MAT (multi-auto-transformation) vector system has been one of the different st...

journal_title：Plant cell reports

authors： Khan RS,Ntui VO,Chin DP,Nakamura I,Mii M

更新日期：2011-04-01 00:00:00

abstract：:A transformed root clone of Datura candida×D. aurea was established following infection with Agrobacterium rhizogenes strain A4. This clone was examined for its growth and hyoscyamine and scopolamine content under various culture conditions. Among the three basal culture media tested, half-strength Gamborg's B5 medium...

journal_title：Plant cell reports

authors： Nussbaumer P,Kapétanidis I,Christen P

更新日期：1998-03-01 00:00:00

abstract：:Mitogen-activated protein kinase (MAPK) signaling cascades are evolutionarily conserved fundamental signal transduction pathways. A MAPK cascade consists of many distinct MAPKKK-MAPKK-MAPK modules linked to various upstream receptors and downstream targets through sequential phosphorylation and activation of the casca...

journal_title：Plant cell reports

authors： Singh R,Jwa NS

更新日期：2013-06-01 00:00:00

abstract：: A high frequency of embryogenesis and transformation from all parts of flowers of two lines of Medicago truncatula R-108-1 and Jemalong J5 were obtained. Using this flower system, we obtained transgenic plants expressing promoter-uidA gene fusions as well as the gfp living cell color reporter gene. Moreover, this met...

journal_title：Plant cell reports

authors： Kamaté K,Rodriguez-Llorente ID,Scholte M,Durand P,Ratet P,Kondorosi E,Kondorosi A,Trinh TH

更新日期：2000-06-01 00:00:00

abstract：:Most infections of plant roots are initiated in the region of elongation; the mechanism for this tissue-specific localization pattern is unknown. In alfalfa expressing PsUGT1 antisense mRNA under the control of the cauliflower mosaic virus (CaMV) 35S promoter, the cell cycle in roots is completed in 48 h instead of 24...

journal_title：Plant cell reports

authors： Woo HH,Hirsch AM,Hawes MC

更新日期：2004-07-01 00:00:00

abstract：:The sequences of the 3' untranslated region (UTR) of the manganese superoxide dismutase (MnSOD) genes in wheat (Triticum aestivum) were found to be quite variable with different predicted thermostabilities. The degradation rates of the 3' UTR variants and the coding region were measured following exposure to endogenou...

journal_title：Plant cell reports

authors： Baek KH,Skinner DZ

更新日期：2006-03-01 00:00:00

abstract：:Tillering is an important trait of cereal crops that optimizes plant architecture for maximum yield. Teosinte Branched 1 (TB1) is a negative regulator of lateral branching and an inducer of female inflorescence formation in Zea mays (maize). Recent studies indicate that TB1 homologs in Oryza sativa (rice), Sorghum bic...

journal_title：Plant cell reports

authors： Choi MS,Woo MO,Koh EB,Lee J,Ham TH,Seo HS,Koh HJ

更新日期：2012-01-01 00:00:00

abstract：KEY MESSAGE:Several hypomethylated sites within the Karma region of EgDEF1 and hotspot regions in chromosomes 1, 2, 3, and 5 may be associated with mantling. One of the main challenges faced by the oil palm industry is fruit abnormalities, such as the "mantled" phenotype that can lead to reduced yields. This clonal abn...

journal_title：Plant cell reports

authors： Sarpan N,Taranenko E,Ooi SE,Low EL,Espinoza A,Tatarinova TV,Ong-Abdullah M

更新日期：2020-09-01 00:00:00

abstract：:Plant regeneration from immature embryos of peanut (Arachis hypogaea L.) can be accomplished through somatic embryogenesis. The highest frequency of somatic embryo formation occurred on B5 medium plus 0.5-1.0 mg/l picloram. Shoots and plants developed from the somatic embryos only after extended culture on basal mediu...

journal_title：Plant cell reports

authors： Ozias-Akins P

更新日期：1989-04-01 00:00:00

abstract：:Tomato has a relatively short growth cycle (fruit ready to pick within 65-85 days from planting) and a relatively high yield (the average for globe tomatoes is 3-9 kg fruit per plant rising to as much as 40 kg fruit per plant). Tomatoes also produce large amounts of important primary and secondary metabolites which ca...

journal_title：Plant cell reports

authors： Li Y,Wang H,Zhang Y,Martin C

更新日期：2018-10-01 00:00:00

abstract：:Microspore-derived embryos from Brassica napus L. were dried to less than 15% moisture and stored dry for a minimum of 7 days. Successful plant regeneration was observed when embryos at the cotyledonary stage of development were treated with 50 uM ABA for 7 days prior to desiccation. Solid agar or liquid medium gave s...

journal_title：Plant cell reports

authors： Senaratna T,Kott L,Beversdorf WD,McKersie BD

更新日期：1991-09-01 00:00:00

abstract：KEY MESSAGE:A novel rice constitutive promoter (P OsCon1 ) was isolated. The molecular mechanism of the promoter activity was investigated. P OsCon1 could be used as an alternative constitutive promoter for crop transgenic engineering. Monocot constitutive promoter is an important resource for crop transgenic engineeri...

journal_title：Plant cell reports

authors： Li J,Xu RF,Qin RY,Ma H,Li H,Zhang YP,Li L,Wei PC,Yang JB

更新日期：2014-10-01 00:00:00

abstract：:In this study, we present a method for transient expression of the type III effector AvrGf1 from Xanthomonas citri subsp. citri strain A(w) in grapefruit leaves (Citrus paradisi) via Agrobacterium tumefaciens. The coding sequence of avrGf1 was placed under the control of the constitutive CaMV 35S promoter in the binar...

journal_title：Plant cell reports

authors： Figueiredo JF,Römer P,Lahaye T,Graham JH,White FF,Jones JB

更新日期：2011-07-01 00:00:00

abstract：:Since plant root systems capture both water and nutrients essential for the formation of crop yield, there has been renewed biotechnological focus on root system improvement. Although water and nutrient uptake can be facilitated by membrane proteins known as aquaporins and nutrient transporters, respectively, there is...

journal_title：Plant cell reports

authors： Ghanem ME,Hichri I,Smigocki AC,Albacete A,Fauconnier ML,Diatloff E,Martinez-Andujar C,Lutts S,Dodd IC,Pérez-Alfocea F

更新日期：2011-05-01 00:00:00

abstract：:Sugarcane white leaf (SCWL)-diseased sugarcane plants collected from Udornthani Province, in north-eastern Thailand, were the source for tissue culture experiments. Explants from axillary buds, meristem tips, and leaves grew optimally in Murashige-Skoog medium containing 0.5 mg/l alpha-naphthaleneacetic acid, 0.5 mg/l...

journal_title：Plant cell reports

authors： Wongkaew P,Fletcher J

更新日期：2004-11-01 00:00:00

abstract：:A selectable marker gene (SMG), usually conferring resistance to an antibiotic or herbicide, is generally introduced into the plant cells with the gene(s) for the trait of interest to allow only the cells that have integrated and express the foreign sequences to regenerate into a plant. The availability of several SMG...

journal_title：Plant cell reports

authors： Rosellini D,Capomaccio S,Ferradini N,Savo Sardaro ML,Nicolia A,Veronesi F

更新日期：2007-07-01 00:00:00

abstract：:We have isolated by affinity chromatography a lectin from the seeds of the winged bean (Psophocrapus tetragonolobus) which agglutinated human (group A, B and O), sheep and rabbit, but not mouse erythrocytes. A molecular weight of 41,000 was obtained from gel filtration, and on sodium dodecyl sulphate polyacrylamide ge...

journal_title：Plant cell reports

authors： Vijayakumar T,Forrester JA

更新日期：1986-12-01 00:00:00

abstract：:We report here an efficient Arabidopsis leafdisc transformation protocol yielding an average transformation frequency of 1.6 transgenic shoots per leaf explant 4 weeks after the bacterial infection period. Subsequent cultivation in vitro is such that a high percentage (85-90%) of the primary transformants produces see...

journal_title：Plant cell reports

authors： van der Graaff E,Hooykaas PJ

更新日期：1996-04-01 00:00:00

abstract：:Hairy root cultures of Lithospermum canescens were established using three strains of Agrobacterium rhizogenes: ATCC 15834, LBA 9402 and NCIB 8196. Eight lines resulting from infection with A. rhizogenes ATCC 15834 demonstrated sufficient biomass increase and were submitted to further investigations. The contents of a...

journal_title：Plant cell reports

authors： Pietrosiuk A,Sykłowska-Baranek K,Wiedenfeld H,Wolinowska R,Furmanowa M,Jaroszyk E

更新日期：2006-10-01 00:00:00

abstract：:This article describes conditions to optimize the yield of viable protoplasts from callus tissue of Asparagus densiflorus cv. Sprengeri and their subsequent regeneration into plantlets. Callus tissue was initiated by culturing spear sections (5-7 mm) on Murashige and Skoog (MS) medium supplemented with 0.8% (wt/vol) B...

journal_title：Plant cell reports

authors： Benmoussa M,Mukhopadhyay S,Desjardins Y

更新日期：1997-12-01 00:00:00

abstract：:In Materials and method section, a sweetpotato variety "Taizhong-6" (China national number 2013003) should be renamed as Ayamurasaki". ...

journal_title：Plant cell reports

authors： Zhong Y,Ahmed S,Deng G,Fan W,Zhang P,Wang H

更新日期：2020-01-01 00:00:00

abstract：:We developed procedures for slow-growth storage of Cedrus atlantica and Cedrus libani microcuttings of juvenile and adult origin, noting factors favouring the extension of subculture intervals. Microcuttings could be stored effectively up to 6 months at 4 degrees C and reduced light intensity, provided that they were ...

journal_title：Plant cell reports

authors： Renau-Morata B,Arrillaga I,Segura J

更新日期：2006-07-01 00:00:00

abstract：:Significantly higher than normal mitotic index (MI) values were induced in Petunia cell suspensions following treatments with colchicine, aphidicolin, drastic medium replacement, or a sequential application of aphidicolin and colchicine. This last treatment yielded the highest MI values: cells incubated with 30 μg/ml ...

journal_title：Plant cell reports

authors： Guri A,Zelcer A,Izhar S

更新日期：1984-12-01 00:00:00

abstract：:Leaf blade tissue of maize inbred lines B73 and Mo17 was analyzed for intraspecific genetic variability in the heat shock response. The maize inbreds were characterized for acquired thermal tolerance and patterns of heat shock protein synthesis. The leakage conductivity assay of membrane stability during stress indica...

journal_title：Plant cell reports

authors： Jorgensen JA,Weng J,Ho TH,Nguyen HT

更新日期：1992-10-01 00:00:00