Abstract:
:Sugarcane white leaf (SCWL)-diseased sugarcane plants collected from Udornthani Province, in north-eastern Thailand, were the source for tissue culture experiments. Explants from axillary buds, meristem tips, and leaves grew optimally in Murashige-Skoog medium containing 0.5 mg/l alpha-naphthaleneacetic acid, 0.5 mg/l 6-benzylaminopurine, and 15% coconut water. Callus development and shoot/root proliferation were more rapid in cultures from diseased than from healthy plants. Disease symptoms continued for 6 years after culture initiation, and SCWL phytoplasma persisted, as confirmed by polymerase chain reaction using both 16S rDNA and 16S-23S rDNA primers. Phytoplasmas in the cultured plantlets were transmissible by grafting to sugarcane and periwinkle, and by feeding of the leafhopper vector Matsumuratettix hiroglyphicus to sugarcane. Although 50% of the plantlets were killed by oxytetracycline at 500 mg/ml, 70-100% of plantlets grown with 200-500 mg/ml oxytetracycline showed symptom remission through 5-8 subcultures. Typical phytoplasma-like bodies, visible by electron microscopy in sieve tubes of untreated diseased plantlets, were absent in antibiotic-treated plantlets. Thus, tissue culture provides a convenient and reliable in vivo system for investigation of SCWL phytoplasma.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Wongkaew P,Fletcher Jdoi
10.1007/s00299-004-0847-2keywords:
subject
Has Abstractpub_date
2004-11-01 00:00:00pages
426-34issue
6eissn
0721-7714issn
1432-203Xjournal_volume
23pub_type
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journal_title:Plant cell reports
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