High plating efficiency with plant cell cultures.

abstract：:The ample supply of O2 proved to be of great importance for berberine production in cell suspension culture ofThalictrum minus, as the specific O2 consumption rate of berberine-producing cells was twice as high as that of non-producing cells. Furthermore, berberine yield increased with increases in the volumetric O2 t...

journal_title：Plant cell reports

authors： Kobayashi Y,Fukui H,Tabata M

更新日期：1989-04-01 00:00:00

abstract：:Plum hypocotyl slices were transformed with the coat protein (CP) gene of plum pox virus (PPV-CP) following cocultivation with Agrobacterium tumefaciens containing the plasmid pGA482GG/PPVCP-33. This binary vector carries the PPV-CP gene construct, as well as the chimeric neomycin phosphotransferase and β-glucuronidas...

journal_title：Plant cell reports

authors： Scorza R,Ravelonandro M,Callahan AM,Cordts JM,Fuchs M,Dunez J,Gonsalves D

更新日期：1994-11-01 00:00:00

abstract：:A cDNA clone encoding a putative RTE1-like protein (Rh-RTH1) was obtained from total RNA isolated from senescing rose (Rosa hybrida cv. Tineke) petals using RT-PCR and RACE techniques. The cDNA (1,061 bp) contained an open reading frame of 684 bp corresponding to 227 amino acids. The amino acid sequence had 60.0, 49.6...

journal_title：Plant cell reports

authors： Yu Y,Wang J,Wang H,Zhang Z,Liu J

更新日期：2010-08-01 00:00:00

abstract：:Hairy root culture of Ginseng (Panax ginseng) was established after roots were induced on callus following infection with Agrobacterium rhizogenes. The transformed cultures of ginseng could be subcultured as an axenic root culture in the absence of phytohormones, and grew with extensive lateral branches more rapidly t...

journal_title：Plant cell reports

authors： Yoshikawa T,Furuya T

更新日期：1987-12-01 00:00:00

abstract：:In a study of the 5'-flanking sequence of the Zea mays L. (maize) Glb1 gene in vitro, serial promoter deletions were generated and linked with the β-glucuronidase (GUS) reporter gene. The promoter deletion-GUS fusions were introduced into the maize P3377 cell line by particle bombardment. GUS assays indicated that tre...

journal_title：Plant cell reports

authors： Liu S,Kriz A,Duncan D,Widholm J

更新日期：1998-05-01 00:00:00

abstract：KEY MESSAGE:We describe a steroid-inducible BABY BOOM system that improves plant regeneration in Arabidopsis leaf cultures and yields fertile plants. Regeneration of Arabidopsis thaliana plants for extended periods of time in tissue culture may result in sterile plants. We report here a novel approach for A. thaliana r...

journal_title：Plant cell reports

authors： Lutz KA,Martin C,Khairzada S,Maliga P

更新日期：2015-10-01 00:00:00

abstract：KEY MESSAGE:Transcription factors from mammals and plants, which play a role in innate immunity, interact with the same microbe-associated molecular pattern (MAMP)-responsive sequences from Arabidopsis thaliana. The interaction of mouse NF-κB p65 with MAMP-responsive sequences containing the core motif GACTTT of the WT...

journal_title：Plant cell reports

authors： Kanofsky K,Riggers J,Staar M,Strauch CJ,Arndt LC,Hehl R

更新日期：2019-09-01 00:00:00

abstract：KEY MESSAGE:This study established a rapid method for the gene expression analysis in potato tubers. The use of microtubers would be useful for primary evaluation of tuber-expressed genes. In the development of transgenic potato or of potato with other genome modifications (e.g., genome editing or RNA-directed DNA meth...

journal_title：Plant cell reports

authors： Wakasa Y,Kasai A,Yamazaki M,Tabei Y,Tsuyama M,Igarashi T,Okazaki T,Yamamoto K,Fujihara H,Kanno A,Noro O,Harada T,Akada S

更新日期：2020-11-01 00:00:00

abstract：KEY MESSAGE:Induced mutations in the waxy locus in rice endosperm did not abolish GBSS activity completely. Compensatory mechanisms in endosperm and leaves caused a major reprogramming of the starch biosynthetic machinery. The mutation of genes in the starch biosynthesis pathway has a profound effect on starch quality ...

journal_title：Plant cell reports

authors： Pérez L,Soto E,Farré G,Juanos J,Villorbina G,Bassie L,Medina V,Serrato AJ,Sahrawy M,Rojas JA,Romagosa I,Muñoz P,Zhu C,Christou P

更新日期：2019-03-01 00:00:00

abstract：:The sequences of the 3' untranslated region (UTR) of the manganese superoxide dismutase (MnSOD) genes in wheat (Triticum aestivum) were found to be quite variable with different predicted thermostabilities. The degradation rates of the 3' UTR variants and the coding region were measured following exposure to endogenou...

journal_title：Plant cell reports

authors： Baek KH,Skinner DZ

更新日期：2006-03-01 00:00:00

abstract：:Molecular analysis of gene expression differences between green and red lettuce leaves was performed using the SSH method. BlastX comparisons of subtractive expressed sequence tags (ESTs) indicated that 7.6% of clones encoded enzymes involved in secondary metabolism. Such clones had a particularly high abundance of fl...

journal_title：Plant cell reports

authors： Park JS,Choung MG,Kim JB,Hahn BS,Kim JB,Bae SC,Roh KH,Kim YH,Cheon CI,Sung MK,Cho KJ

更新日期：2007-04-01 00:00:00

abstract：:Time-course changes in anthocyanin accumulation, phenylalanine ammonia-lyase activity and chalcone synthase activity were examined in roselle callus tissues incubated under different culture conditions. Phenylalanine ammonia-lyase activity was not affected by either the kind of auxin supplemented to the medium or ligh...

journal_title：Plant cell reports

authors： Mizukami H,Tomita K,Ohashi H

更新日期：1989-12-01 00:00:00

abstract：:Two modes of endocytosis are known to occur in eucaryotic cells: fluid phase and receptor-mediated endocytosis. Fluid-phase endocytosis in plant cells resembles the retrieval of excess plasma membrane material previously incorporated by exocytosis. Pollen tubes need to carry out strong membrane retrieval due to their ...

journal_title：Plant cell reports

authors： Lisboa S,Scherer GE,Quader H

更新日期：2008-01-01 00:00:00

abstract：:Innovations in plant breeding like genome editing methods raised questions about the adequacy of established regulatory policies for plant breeding and biotechnology in view of these new breeding methods and the resulting products. Most countries follow the principle approach that only those plants will be regulated u...

journal_title：Plant cell reports

authors： Jorasch P

更新日期：2020-01-01 00:00:00

abstract：KEY MESSAGE:Functional characterization of two tobacco genes, one involved in xylan synthesis and the other, a positive regulator of secondary cell wall formation, is reported. Lignocellulosic secondary cell walls (SCW) provide essential plant materials for the production of second-generation bioethanol. Therefore, tho...

journal_title：Plant cell reports

authors： Pandey SK,Nookaraju A,Fujino T,Pattathil S,Joshi CP

更新日期：2016-11-01 00:00:00

abstract：:Embryonal-suspensor tissue (EST) of Mediterranean cypress (Cupressus sempervirens L.) was tested for microprojectile-DNA delivery (by the PDS-1000/He device) for different subculture periods (9, 15, and 21 days) using the plasmid vectors pRT99GUS [containing the β-glucuronidase (GUS) and neomycin phosphotransferase (N...

journal_title：Plant cell reports

authors： Lambardi M,Lachance D,Séguin A,Charest PJ

更新日期：1998-12-01 00:00:00

abstract：:Expiants of five plant species (Allium cepa, Antirrhinum majus, Brassica campestris. Glycine max, and Nicotiana tabacum) were co-cultivated with three Agrobacterium tumefaciens strains under different conditions to assess the effects of acetosyringone and medium pH on strain virulence. Tumours were incited on all dico...

journal_title：Plant cell reports

authors： Godwin I,Todd G,Ford-Lloyd B,Newbury HJ

更新日期：1991-04-01 00:00:00

abstract：KEY MESSAGE:Tetraploid clementine displays mainly tetrasomic inheritance. Genetic structures of 2n SDR and 2 × gametes from DD clementine are complementary and will guides triploids citrus breeding strategies. Triploid breeding is developed worldwide to create new seedless cultivars. Citrus triploid hybrids can be reco...

journal_title：Plant cell reports

authors： Aleza P,Cuenca J,Juárez J,Navarro L,Ollitrault P

更新日期：2016-08-01 00:00:00

abstract：:Callus cultures and cell suspension cultures derived from Ginkgo biloba L. leaves produced ginkgolidc B. In cell suspension cultures, the production reached a maximum by the 13th day of subculture and followed by a sharp decrease. The medium of Murashige and Skoog induced the highest ginkgolide B content in cultures w...

journal_title：Plant cell reports

authors： Jeon MH,Sung SH,Huh H,Kim YC

更新日期：1995-05-01 00:00:00

abstract：:Taro (Colocasia esculenta) is one of the most important crops in the Pacific Islands, however, taro yields have been declining in Hawaii over the past 30 years partly due to diseases caused by oomycete and fungal pathogens. In this study, an efficient Agrobacterium tumefaciens-mediated transformation method for taro i...

journal_title：Plant cell reports

authors： He X,Miyasaka SC,Fitch MM,Moore PH,Zhu YJ

更新日期：2008-05-01 00:00:00

abstract：:Somatic embroys and subsequent plant regeneration were obtained from isolated leaf epidermis of Gaillardia picta. Abaxial and adaxial epidermal peels (monolayer) from 45 days old aseptic seedlings were isolated and segments measuring 5 mm x 3 mm were cultured on B5 basal medium supplemented with various growth regulat...

journal_title：Plant cell reports

authors： Pillai KG,Rao IU,Rao IV,Ram HY

更新日期：1992-01-01 00:00:00

abstract：KEY MESSAGE:Reciprocal effects were found for tomato fruit quality and DNA methylation. The epigenetic identity of reciprocal hybrids indicates that DNA methylation might be one of the mechanisms involved in POEs. Crosses between different genotypes and even between different species are commonly used in plant breeding...

journal_title：Plant cell reports

authors： Gimenez MD,Vazquez DV,Trepat F,Cambiaso V,Rodríguez GR

更新日期：2021-01-01 00:00:00

abstract：:Independent carrot (Daucus carota) hairy root lines were established by inoculation of discs taken from the same carrot with Agrobacterium rhizogenes 8196 and A. tumefaciens C58C1(pRi8196) carrying pRi8196. Several lines were compared with respect to T-DNA length. One of them was found to have integrated sequences cov...

journal_title：Plant cell reports

authors： David C,Petit A,Tempé J

更新日期：1988-03-01 00:00:00

abstract：KEY MESSAGE:We provide evidence that nucleotide sequence and methylation status changes occur in the Arabidopsis genome during in vitro tissue culture at a frequency high enough to represent an important source of variation. Somaclonal variation is a general consequence of the tissue culture process that has to be anal...

journal_title：Plant cell reports

authors： Coronel CJ,González AI,Ruiz ML,Polanco C

更新日期：2018-01-01 00:00:00

abstract：:A biolistic particle delivery system was used to genetically transform embryogenic tissue of Pinus radiata. The introduced DNA contained a uidA reporter gene under the control of either the tandem CaMV 35S or the artificial Emu promoter, and the npt II selectable marker controlled by the CaMV 35S promoter. The average...

journal_title：Plant cell reports

authors： Walter C,Grace LJ,Wagner A,White DWR,Walden AR,Donaldson SS,Hinton H,Gardner RC,Smith DR

更新日期：1998-04-01 00:00:00

abstract：:While searching for genes expressed in acid lemon but not in acidless lime pulp, we isolated clone Cl111 which showed the following expression phenotypes: (1) while it was expressed in the ovaries in both varieties, its mRNA was detected only in the pulp of the acid fruit, (2) no or very low expression of the gene was...

journal_title：Plant cell reports

authors： Sorkina A,Bardosh G,Liu YZ,Fridman I,Schlizerman L,Zur N,Or E,Goldschmidt EE,Blumwald E,Sadka A

更新日期：2011-09-01 00:00:00

abstract：:A method has been devised which allows the rapid production of fine suspension cultures of small aggregate size from suspension cultures of large average aggregate size, such as those of Capsicum frutescens. The method, which uses a Waring blender for aseptic homogenisation of cultures, has also been shown to be effec...

journal_title：Plant cell reports

authors： Williams PD,Wilkinson AK,Lewis JA,Black GM,Mavituna F

更新日期：1988-10-01 00:00:00

abstract：:Six sugarbeet (Beta vulgaris L.) lines (GWI-248, SPB-11, MonoHy 55, SMS-1, EL45 and FC607) were tested for regeneration. Shoot cultures were initiated in vitro from naked, sterilized embryos obtained from mature seed. Excised petioles from cultured shoots were plated on Gamborg's B5 medium and four modified Murashige ...

journal_title：Plant cell reports

authors： Freytag AH,Anand SC,Rao-Arelli AP,Owens LD

更新日期：1988-01-01 00:00:00

abstract：:Rice plants (Oryza sativa L., Chinsurah Boro II var. Indica) were regenerated from protoplasts isolated from microspore derived cell suspensions. A simple procedure for the establishment of such cell suspension cultures from embryogenic microcallus derived from cultured isolated microspores of Indica-type rice is desc...

journal_title：Plant cell reports

authors： Datta SK,Datta K,Potrykus I

更新日期：1990-09-01 00:00:00

abstract：:Three different regeneration systems, viz. direct regeneration of adventitious shoot buds from explant, regeneration through callus cultures and somatic embryos were compared to see their effect on transfer of neomycin phosphotransferase (nptII) and beta-glucuronidase (GUS) reporter gene (gus) to Morus alba clone M5, ...

journal_title：Plant cell reports

authors： Agarwal S,Kanwar K

更新日期：2007-02-01 00:00:00