Ginkgolide B production in cultured cells derived from Ginkgo biloba L. leaves.

Abstract:

:Callus cultures and cell suspension cultures derived from Ginkgo biloba L. leaves produced ginkgolidc B. In cell suspension cultures, the production reached a maximum by the 13th day of subculture and followed by a sharp decrease. The medium of Murashige and Skoog induced the highest ginkgolide B content in cultures while the medium of Schenk and Hildebrandt promoted cell growth. For the maximal production of ginkgolide B, cells were cultured in Murashige and Skoog medium modified to contain 1.0 mg/l of α-naphthaleneacetic acid, 0.1 mg/1 of kinetin, 30 g/1 sucrose and 1.25 mM potassium phosphate with a molar ratio of ammonium to nitrate ions of 1 ∶ 3.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Jeon MH,Sung SH,Huh H,Kim YC

doi

10.1007/BF00232783

subject

Has Abstract

pub_date

1995-05-01 00:00:00

pages

501-4

issue

8

eissn

0721-7714

issn

1432-203X

journal_volume

14

pub_type

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